##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211530_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	10219603
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.134886159472146	33.0	31.0	34.0	30.0	34.0
2	32.35150915353561	34.0	31.0	34.0	31.0	34.0
3	32.36524598851834	34.0	31.0	34.0	30.0	34.0
4	35.99696710332094	37.0	35.0	37.0	35.0	37.0
5	35.92098215556906	37.0	35.0	37.0	35.0	37.0
6	35.94540443498637	37.0	35.0	37.0	35.0	37.0
7	35.86968084768068	37.0	35.0	37.0	35.0	37.0
8	35.867024873666814	37.0	35.0	37.0	35.0	37.0
9	37.52256433053221	39.0	37.0	39.0	35.0	39.0
10-11	37.487120390097346	39.0	37.0	39.0	35.0	39.0
12-13	37.39928874927921	39.0	37.0	39.0	34.0	39.0
14-15	38.63812195052978	40.0	38.0	41.0	34.0	41.0
16-17	38.569967884271044	40.0	38.0	41.0	34.0	41.0
18-19	38.561822949482476	40.0	38.0	41.0	34.0	41.0
20-21	38.503822359831396	40.0	38.0	41.0	34.0	41.0
22-23	38.399876149787815	40.0	38.0	41.0	34.0	41.0
24-25	38.289151447468164	40.0	38.0	41.0	34.0	41.0
26-27	38.11128074153174	40.0	38.0	41.0	33.0	41.0
28-29	38.14918994407121	40.0	38.0	41.0	33.5	41.0
30-31	38.069487826484064	40.0	37.5	41.0	33.0	41.0
32-33	37.887444062161705	40.0	37.0	41.0	33.0	41.0
34-35	37.77703429379791	40.0	37.0	41.0	33.0	41.0
36-37	37.72177094354839	40.0	37.0	41.0	33.0	41.0
38-39	37.62763015353923	40.0	37.0	41.0	32.5	41.0
40-41	37.46879453145098	40.0	36.5	41.0	32.0	41.0
42-43	37.315557316658975	40.0	36.0	41.0	31.0	41.0
44-45	37.12339099669527	39.5	36.0	41.0	31.0	41.0
46-47	36.857833273953986	39.0	36.0	41.0	30.0	41.0
48-49	36.625458542763354	39.0	35.0	41.0	30.0	41.0
50-51	36.42069393497967	39.0	35.0	40.5	29.5	41.0
52-53	36.21881813804313	39.0	35.0	40.0	28.5	41.0
54-55	36.02212742510643	39.0	35.0	40.0	28.0	41.0
56-57	35.81886571327673	38.0	34.5	40.0	28.0	41.0
58-59	35.556776275947314	38.0	34.0	40.0	27.5	41.0
60-61	35.24284837679116	38.0	34.0	40.0	26.5	41.0
62-63	34.91673570881373	37.0	34.0	40.0	26.0	41.0
64-65	34.596516224749635	37.0	33.0	39.5	26.0	41.0
66-67	34.24784920705824	36.0	33.0	39.0	26.0	41.0
68-69	33.84743311457402	36.0	32.5	39.0	25.0	40.0
70-71	33.45919885537628	35.0	32.0	38.5	24.5	40.0
72-73	33.05265468727112	35.0	31.5	37.5	24.0	40.0
74-75	32.64368229372511	35.0	31.0	37.0	23.0	39.0
76-77	31.103321772871215	33.5	29.5	35.5	21.0	38.0
78-79	31.935755185402016	34.0	31.0	36.0	22.5	38.5
80-81	31.90610031524708	34.0	31.0	36.0	23.0	37.5
82-83	31.629411093562048	34.0	31.0	35.0	22.0	37.0
84-85	31.33707410160649	34.0	30.5	35.0	20.5	37.0
86-87	31.044450943935885	34.0	30.0	35.0	20.0	36.0
88-89	30.77124605525283	34.0	30.0	35.0	20.0	36.0
90-91	30.49060007516926	34.0	29.5	35.0	19.0	35.5
92-93	30.28385598736076	34.0	29.0	35.0	19.0	35.0
94-95	30.063985557951714	34.0	29.0	35.0	18.0	35.0
96-97	29.860308174397773	34.0	29.0	35.0	18.0	35.0
98-99	29.70213236267593	34.0	29.0	35.0	18.0	35.0
100	27.799432228433922	31.0	25.0	34.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.30984161688581224
1101	2	0.2094336806685817
1101	3	0.2742642360473653
1101	4	0.16763993973471258
1101	5	0.17694810031220953
1101	6	0.17909168065413894
1101	7	0.2285566106081589
1101	8	0.1760718362294469
1101	9	0.2020256361500472
1101	10-11	0.23516297107032358
1101	12-13	0.2299823113548669
1101	14-15	0.2863204218504336
1101	16-17	0.28338826537977013
1101	18-19	0.3184371780411226
1101	20-21	0.3071010729769412
1101	22-23	0.2740780963100278
1101	24-25	0.2885924090893468
1101	26-27	0.2314918927931089
1101	28-29	0.30546649953713256
1101	30-31	0.3545205520911807
1101	32-33	0.398565242224052
1101	34-35	0.37174853545221964
1101	36-37	0.3737337174136499
1101	38-39	0.34205957307137425
1101	40-41	0.3582549264624717
1101	42-43	0.3310872430727727
1101	44-45	0.33802834899427836
1101	46-47	0.42467289767512995
1101	48-49	0.41168809697239794
1101	50-51	0.4062913051999999
1101	52-53	0.40716256902521764
1101	54-55	0.3948120600221898
1101	56-57	0.35136660985428136
1101	58-59	0.4426930304054153
1101	60-61	0.3926628879081733
1101	62-63	0.36528488594328223
1101	64-65	0.34384411623398137
1101	66-67	0.26489589597382235
1101	68-69	0.30519958197282193
1101	70-71	0.3305333997322535
1101	72-73	0.22971368019769756
1101	74-75	0.2105407732989235
1101	76-77	0.23018139253363756
1101	78-79	0.3672310394600906
1101	80-81	0.32459785419357345
1101	82-83	0.22256956760205782
1101	84-85	0.30107333642521894
1101	86-87	0.2863740000947992
1101	88-89	0.1938627071172334
1101	90-91	0.16762976294613807
1101	92-93	0.13123073927265594
1101	94-95	0.2251731476915957
1101	96-97	0.20640002926228362
1101	98-99	0.05251306140059597
1101	100	0.15223169864268016
1102	1	0.2869819474868933
1102	2	0.22894431987918296
1102	3	0.3110957105945218
1102	4	0.1727771968095766
1102	5	0.16398742439278635
1102	6	0.1448187923450206
1102	7	0.16279741875353437
1102	8	0.15848230422421494
1102	9	0.19737013347439358
1102	10-11	0.22121374959689888
1102	12-13	0.18780781991760165
1102	14-15	0.2861275637068488
1102	16-17	0.30193257091613646
1102	18-19	0.27675066952846095
1102	20-21	0.2987882491875311
1102	22-23	0.29750791917336983
1102	24-25	0.27372895124448604
1102	26-27	0.2943169547418307
1102	28-29	0.32168445659122824
1102	30-31	0.3283748928467318
1102	32-33	0.39596006278318896
1102	34-35	0.3437384849963365
1102	36-37	0.4471421440465946
1102	38-39	0.4082143050350382
1102	40-41	0.3946895577014189
1102	42-43	0.36234502298010085
1102	44-45	0.3767984204460717
1102	46-47	0.40530946630821063
1102	48-49	0.3973079228692882
1102	50-51	0.47239218054200904
1102	52-53	0.4096690962898464
1102	54-55	0.3839818885821984
1102	56-57	0.3818945752356697
1102	58-59	0.4314140870037235
1102	60-61	0.4095450664082563
1102	62-63	0.36985217353477395
1102	64-65	0.47725727485971703
1102	66-67	0.43796634020497294
1102	68-69	0.5088801046311175
1102	70-71	0.46229322025015307
1102	72-73	0.49192348342596404
1102	74-75	0.35376819062833675
1102	76-77	0.41495157934156524
1102	78-79	0.4269405891778
1102	80-81	0.3840283583081039
1102	82-83	0.39721604340043726
1102	84-85	0.3499488089384002
1102	86-87	0.31373280360220335
1102	88-89	0.31004837012961417
1102	90-91	0.33165101453676726
1102	92-93	0.24520913170934477
1102	94-95	0.29685669441587237
1102	96-97	0.31923718336601326
1102	98-99	0.2682536113745577
1102	100	0.40239059694938817
1103	1	0.3469271640283793
1103	2	0.23980547616464065
1103	3	0.33302603574845335
1103	4	0.19168912250832904
1103	5	0.18601892193186131
1103	6	0.19308715795719422
1103	7	0.19470521493020243
1103	8	0.20398436081126903
1103	9	0.2302718480879946
1103	10-11	0.24220041477579457
1103	12-13	0.26994809087992877
1103	14-15	0.36645232243635206
1103	16-17	0.3332510186711062
1103	18-19	0.3418928385140205
1103	20-21	0.3332438621362215
1103	22-23	0.3298787795060889
1103	24-25	0.3148549590211118
1103	26-27	0.3346815593515515
1103	28-29	0.3377612074123135
1103	30-31	0.4206001266353354
1103	32-33	0.45693507848631043
1103	34-35	0.41639072948294853
1103	36-37	0.4675457339543527
1103	38-39	0.468321142919514
1103	40-41	0.5153708974227484
1103	42-43	0.4797325769491465
1103	44-45	0.4334107356160999
1103	46-47	0.5886138558826559
1103	48-49	0.5353202463906541
1103	50-51	0.5036654978561188
1103	52-53	0.4517102938855615
1103	54-55	0.47258546329096873
1103	56-57	0.565095243837078
1103	58-59	0.5517513365129005
1103	60-61	0.4932249736381138
1103	62-63	0.5890803631841024
1103	64-65	0.5474711723288337
1103	66-67	0.5979468822568563
1103	68-69	0.5547078522450306
1103	70-71	0.6264492746443366
1103	72-73	0.5199448912558964
1103	74-75	0.5257727622012638
1103	76-77	0.5573215062202266
1103	78-79	0.5405469563446452
1103	80-81	0.558364775998303
1103	82-83	0.5556167385595963
1103	84-85	0.4517319915047011
1103	86-87	0.4772426155306846
1103	88-89	0.4431871728199823
1103	90-91	0.5657563797924077
1103	92-93	0.5266990076656022
1103	94-95	0.5505973216561308
1103	96-97	0.5093174970435896
1103	98-99	0.5074061677258221
1103	100	0.6814269037625102
1104	1	0.26049522988127904
1104	2	0.18736410776996593
1104	3	0.2407285997611197
1104	4	0.09779489862194879
1104	5	0.12004829101606163
1104	6	0.16524181916201997
1104	7	0.19490283238667416
1104	8	0.174417927635794
1104	9	0.21323896214585858
1104	10-11	0.21270617522278457
1104	12-13	0.2545398042602258
1104	14-15	0.3168746580889561
1104	16-17	0.2557915574530796
1104	18-19	0.2773154714693291
1104	20-21	0.22685889786663438
1104	22-23	0.25463866118830936
1104	24-25	0.3276175319836483
1104	26-27	0.335200133426234
1104	28-29	0.36803139910188065
1104	30-31	0.29338145691051665
1104	32-33	0.25532132498727833
1104	34-35	0.43629604508930697
1104	36-37	0.29917896571518554
1104	38-39	0.38025544162984204
1104	40-41	0.4841743768671378
1104	42-43	0.494579549764147
1104	44-45	0.45531769195429916
1104	46-47	0.4274907987044472
1104	48-49	0.4376402142108944
1104	50-51	0.3911881814989755
1104	52-53	0.39821899687906637
1104	54-55	0.417762139661626
1104	56-57	0.473947832317009
1104	58-59	0.4611914669754853
1104	60-61	0.4514200454254649
1104	62-63	0.43832466553391924
1104	64-65	0.5347763828013754
1104	66-67	0.5605447779539077
1104	68-69	0.5110842843615941
1104	70-71	0.4633673875326636
1104	72-73	0.4530964356387628
1104	74-75	0.5330359538929628
1104	76-77	0.40475673154368863
1104	78-79	0.5252887621809457
1104	80-81	0.6287745586072511
1104	82-83	0.48052417792584023
1104	84-85	0.5520164466167756
1104	86-87	0.5544276106913237
1104	88-89	0.5387274626257543
1104	90-91	0.4726574346918575
1104	92-93	0.5270904759547754
1104	94-95	0.5137589395860793
1104	96-97	0.5498953745171349
1104	98-99	0.5551702579981317
1104	100	0.7038933221794537
1105	1	0.2681656316105858
1105	2	0.17985821713795502
1105	3	0.23795462953427204
1105	4	0.129383997779577
1105	5	0.15925558021742603
1105	6	0.14196768827831363
1105	7	0.17665914539230698
1105	8	0.1610583433873174
1105	9	0.1537044021515257
1105	10-11	0.2294597703959269
1105	12-13	0.2428056049741869
1105	14-15	0.3559559665497076
1105	16-17	0.3683132099814159
1105	18-19	0.3417668353386176
1105	20-21	0.2804385726833516
1105	22-23	0.2976121341568927
1105	24-25	0.38532469495290655
1105	26-27	0.3598811190698612
1105	28-29	0.4222617928718364
1105	30-31	0.4210169440800158
1105	32-33	0.45837631665183665
1105	34-35	0.4702314502570104
1105	36-37	0.49791276411315266
1105	38-39	0.497488654160513
1105	40-41	0.5281759963151416
1105	42-43	0.4891772801525818
1105	44-45	0.5264308096603116
1105	46-47	0.5486270322305273
1105	48-49	0.5911452868837728
1105	50-51	0.6426795181655223
1105	52-53	0.6443144677056623
1105	54-55	0.5492486994736367
1105	56-57	0.6195395669079389
1105	58-59	0.6142291121992614
1105	60-61	0.6764358441883616
1105	62-63	0.6494292699342807
1105	64-65	0.681888695954278
1105	66-67	0.6640547797354728
1105	68-69	0.6961335340334642
1105	70-71	0.7540705259648703
1105	72-73	0.6953325029349173
1105	74-75	0.7368367036632932
1105	76-77	0.647394752212815
1105	78-79	0.7874859481697989
1105	80-81	0.7134587047216421
1105	82-83	0.7326508046454201
1105	84-85	0.7560667759046993
1105	86-87	0.7132632786123629
1105	88-89	0.7205033316726244
1105	90-91	0.8325192597337292
1105	92-93	0.8481712755610609
1105	94-95	0.8259027432559698
1105	96-97	0.8558247547292339
1105	98-99	0.8697887303823642
1105	100	0.923995141908712
1106	1	0.22052822679060569
1106	2	0.14656718919330558
1106	3	0.20163234574255995
1106	4	0.14377155516791618
1106	5	0.12401321764026108
1106	6	0.11425744767812063
1106	7	0.13283443601643086
1106	8	0.13436411872783793
1106	9	0.10516761622579907
1106	10-11	0.13794934579334495
1106	12-13	0.1852591818182603
1106	14-15	0.22204134519673602
1106	16-17	0.21133957903097667
1106	18-19	0.23440507034280955
1106	20-21	0.29082847942240164
1106	22-23	0.29128545929211214
1106	24-25	0.32640795654498334
1106	26-27	0.3047659166252785
1106	28-29	0.32315202158015666
1106	30-31	0.32653526511494846
1106	32-33	0.4456265368344816
1106	34-35	0.45298360026692563
1106	36-37	0.3751227978287517
1106	38-39	0.3663957136058329
1106	40-41	0.4067775482961835
1106	42-43	0.438397625260869
1106	44-45	0.5178349740928567
1106	46-47	0.5085718250212139
1106	48-49	0.48649085655935664
1106	50-51	0.5020986206871285
1106	52-53	0.5012857969624704
1106	54-55	0.4635417364657073
1106	56-57	0.5337876129315546
1106	58-59	0.6222049757113624
1106	60-61	0.5820708974500945
1106	62-63	0.576808506537823
1106	64-65	0.5575997232856906
1106	66-67	0.577158065932295
1106	68-69	0.5637441702214261
1106	70-71	0.6021819254694307
1106	72-73	0.5242043489024582
1106	74-75	0.5816393658170895
1106	76-77	0.5426848775299682
1106	78-79	0.585663950701953
1106	80-81	0.5614993936980888
1106	82-83	0.5653079546042825
1106	84-85	0.6156698231912401
1106	86-87	0.4768880428815194
1106	88-89	0.5703448564949838
1106	90-91	0.6228666018594993
1106	92-93	0.6305981944751657
1106	94-95	0.7253933052488826
1106	96-97	0.7144448059641562
1106	98-99	0.7502476014139745
1106	100	0.7720094336406511
1107	1	0.22022685585479707
1107	2	0.20110143570219918
1107	3	0.26795526473240017
1107	4	0.15558560422520884
1107	5	0.13612446174104775
1107	6	0.15698803667712724
1107	7	0.171614791501149
1107	8	0.17063339773850572
1107	9	0.1900498870606171
1107	10-11	0.18950942543210658
1107	12-13	0.2190756300220542
1107	14-15	0.26854002653025333
1107	16-17	0.25042808324595
1107	18-19	0.22072716796674996
1107	20-21	0.22689919796593472
1107	22-23	0.281787071009866
1107	24-25	0.3064884362689284
1107	26-27	0.347511924303582
1107	28-29	0.3115378371532884
1107	30-31	0.20527335928459678
1107	32-33	0.2582051981982332
1107	34-35	0.23182597449812192
1107	36-37	0.35475747695687687
1107	38-39	0.3119362798137928
1107	40-41	0.27510193071142197
1107	42-43	0.31682885559020235
1107	44-45	0.374809321361532
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2316	76-77	-0.3993689128492868
2316	78-79	-0.2591566917108885
2316	80-81	-0.16644267677105518
2316	82-83	-0.07389941732742145
2316	84-85	-0.13965981271476835
2316	86-87	-0.148046026514276
2316	88-89	-0.08530921654396195
2316	90-91	-0.04414962523233257
2316	92-93	0.06402908233862448
2316	94-95	0.13283451421137116
2316	96-97	0.16302388461665984
2316	98-99	-0.006497312914749642
2316	100	-0.21863460378460786
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	4.0
10	16.0
11	51.0
12	115.0
13	325.0
14	956.0
15	2454.0
16	5438.0
17	11159.0
18	20371.0
19	31889.0
20	46085.0
21	64022.0
22	85655.0
23	98457.0
24	104680.0
25	113495.0
26	130883.0
27	154894.0
28	186245.0
29	225596.0
30	271641.0
31	330524.0
32	405485.0
33	509939.0
34	674393.0
35	927430.0
36	1362689.0
37	2100374.0
38	2039674.0
39	314636.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.60761270162046	19.97530545989471	13.746245693060253	23.670836145424577
2	15.110858658282847	21.296222767578644	39.87193774333087	23.720980830807637
3	17.30748399607927	26.867535063021997	30.915364282515228	24.909616658383506
4	11.95481124677505	17.532394334223067	38.186956469835124	32.32583794916676
5	12.417292386251528	38.62052438520568	35.51817057578727	13.444012652755525
6	30.7081795552266	38.20223432735384	17.78054415555454	13.30904196186502
7	26.810033618722763	33.17811856292265	22.58168932785354	17.43015849050105
8	25.876992256864156	36.365124605374646	20.170315523171723	17.587567614589478
9	26.70321473411731	14.94860709336891	20.109004255645598	38.23917391686818
10-11	24.54963270099631	26.506230232231136	28.9595006772768	19.984636389495755
12-13	25.151270553269043	24.354439208646365	28.999057008378898	21.495233229705693
14-15	22.478143231199883	25.705915386341328	26.471321831190508	25.344619551268284
16-17	21.9204482084354	28.733740325265416	27.167552992402488	22.178258473896697
18-19	21.835851957735116	27.67586341426374	29.250431564634688	21.23785306336645
20-21	22.592548829943045	26.857898572475964	29.280851098182918	21.268701499398073
22-23	22.38904595359751	26.719610669788775	29.07778947370996	21.813553902903756
24-25	22.145650409720027	26.906521443768767	29.154197368086944	21.793630778424262
26-27	22.114998337750098	27.021549070130803	29.096663195389834	21.76678939672927
28-29	22.034838680511175	27.016866671500562	29.27521035024893	21.67308429773933
30-31	22.325004131836355	26.918157935860787	29.178035128584018	21.578802803718837
32-33	22.12872853764372	27.056669701450826	29.089614658085765	21.724987102819686
34-35	22.280181225598312	27.155618350229393	29.110591033257847	21.45360939091444
36-37	22.355551532735294	27.101930382920848	28.944877305341027	21.597640779002838
38-39	22.252745733880154	27.221034372269454	28.932252628799933	21.593967265050463
40-41	22.272443266469615	27.069667468926887	29.01692379278103	21.640965471822465
42-43	22.137946644469935	27.138640455421815	28.864781431935775	21.858631468172472
44-45	22.136660687335105	27.288752982041164	28.76160413876342	21.812982191860314
46-47	22.135221346390935	27.271426210962566	28.56343569943477	22.029916743211725
48-49	22.152258585002958	27.39124592989671	28.500279046663472	21.956216438436865
50-51	22.204766250192662	27.521432468336315	28.31149189355815	21.962309387912875
52-53	22.249939109721005	27.59279810623159	28.1471565144868	22.010106269560605
54-55	22.127474985209435	27.53912331444825	28.274575868494235	22.05882583184808
56-57	22.233793983593042	27.566339528199617	28.053637022103818	22.146229466103524
58-59	22.170071189177285	27.537759620324387	28.2303740489762	22.06179514152213
60-61	22.355535805556144	27.621066251407818	28.197427752857052	21.82597019017899
62-63	22.13655099150315	27.642076794855452	28.23976537936363	21.981606834277766
64-65	22.08620657852318	27.427217088290156	28.270271616436833	22.216304716749836
66-67	22.085680160217876	27.48766096284368	28.14881903903525	22.277839837903194
68-69	22.04595168061131	27.554043739379676	28.175975689938475	22.224028890070535
70-71	22.002004098224297	27.434607488359987	28.15240834284171	22.410980070574006
72-73	21.97946125148757	27.689497947465814	27.876642641413678	22.454398159632937
74-75	21.952244594850733	27.758948450688763	27.84700746617938	22.441799488281124
76-77	21.81800505769833	27.58496617127265	28.01890604155501	22.578122729474003
78-79	21.834008552190472	27.530551453497093	27.983222663789153	22.652217330523282
80-81	21.83538731209917	27.762182897278226	27.739005454307485	22.663424336315117
82-83	21.905912542058378	27.71144183661286	27.780751306036244	22.601894315292515
84-85	21.780233744163883	27.758621735831007	27.746383334713272	22.71476118529184
86-87	21.66551168438991	27.73109918116071	27.852686737046774	22.75070239740261
88-89	21.615282301633286	27.554558676145884	27.910450171471524	22.919708850749306
90-91	21.80336463387295	27.58625600272562	27.80649398639804	22.803885377003393
92-93	21.704401831146722	27.526758712120618	27.756033802291906	23.012805654440747
94-95	21.521297568844876	27.419912644434262	27.829913114298353	23.2288766724225
96-97	21.490435598785005	27.35622323477352	27.88316695726813	23.27017420917335
98-99	21.316883789636478	27.327698009459127	27.824930741849684	23.53048745905471
100	20.476655538675388	27.843032784919853	27.973834230283163	23.7064774461216
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	4286.0
1	3158.0
2	3063.5
3	4216.5
4	3404.5
5	2987.0
6	4734.0
7	9594.0
8	15488.5
9	18145.5
10	15214.5
11	11486.0
12	11421.0
13	12144.5
14	12924.0
15	13599.0
16	14211.5
17	15007.0
18	16279.5
19	17726.5
20	19260.0
21	21823.5
22	25721.0
23	31343.0
24	38835.5
25	48129.5
26	60191.5
27	73744.0
28	88290.5
29	105232.0
30	124608.5
31	142862.5
32	162734.0
33	185626.0
34	209086.5
35	230288.5
36	255769.5
37	281608.5
38	299559.5
39	314759.5
40	321905.0
41	327574.5
42	335390.5
43	343835.5
44	352938.5
45	364992.5
46	374835.0
47	379016.5
48	387425.5
49	392399.5
50	391778.5
51	383240.5
52	365193.5
53	348654.0
54	331405.0
55	306867.5
56	277052.0
57	245909.5
58	215638.0
59	183750.5
60	151711.0
61	123947.0
62	99525.5
63	76877.0
64	56191.5
65	40710.0
66	29916.5
67	22086.0
68	16355.0
69	12049.0
70	9100.5
71	6881.5
72	5054.5
73	3700.0
74	2748.5
75	1994.5
76	1402.5
77	898.0
78	608.5
79	434.5
80	321.0
81	232.0
82	162.5
83	117.5
84	92.0
85	69.0
86	43.5
87	32.0
88	25.5
89	19.5
90	13.0
91	11.0
92	11.0
93	7.0
94	5.0
95	3.0
96	2.5
97	5.5
98	6.0
99	5.0
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0033269394124214023
2	0.06765429146318111
3	7.436688098353723E-4
4	6.751729984031669E-4
5	0.0016536845902918144
6	6.066771869709616E-4
7	0.0
8	9.785115918886477E-4
9	1.9570231837772955E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	7.045283461598263E-4
18-19	0.001002974381685864
20-21	0.0032829063907864127
22-23	4.892557959443239E-6
24-25	2.4462789797216194E-5
26-27	2.0059487633717276E-4
28-29	9.785115918886477E-4
30-31	0.00172218040172402
32-33	0.0030823115144492405
34-35	0.003977649621027353
36-37	0.0012524948376174691
38-39	0.0015509408731435067
40-41	0.005460094682738654
42-43	0.00666366394076169
44-45	0.0050099793504698766
46-47	0.0066196309191267014
48-49	0.008630472240457874
50-51	0.008591331776782327
52-53	0.003933616599392364
54-55	0.002436493863802733
56-57	0.0027496175732071
58-59	0.003454145919366927
60-61	0.007397547634678177
62-63	0.013752980423994943
64-65	0.003860228230000716
66-67	0.009961248005426433
68-69	0.012549411165971906
70-71	0.005313317943955357
72-73	0.007554109489380361
74-75	0.003131237094043673
76-77	0.0032290882532325376
78-79	0.007999332263689695
80-81	0.006022738848074627
82-83	0.016272647773108214
84-85	0.017270729596834632
86-87	0.022256246157507292
88-89	0.02116520573255145
90-91	0.0242034842253657
92-93	0.03415983967283269
94-95	0.03799560511303619
96-97	0.02238834522241226
98-99	0.018126927239737196
100	0.017867621667886708
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.0219603E7
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.7712265631044
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.78444793258734	36.298396858686246
2	17.042779483694392	17.987367547981282
3	6.357450344441346	10.06471357470601
4	2.9145499755092095	6.152175083483474
5	1.5858397978730814	4.184335563317402
6	0.9067304606910425	2.8709567143677015
7	0.5654750343789973	2.088856780849532
8	0.3814692534194221	1.6104480319243688
9	0.27503075240205277	1.3062339132146816
>10	1.1110382793386073	9.632311461625363
>50	0.045079342920568095	1.6295490506717702
>100	0.02680547494048989	2.6690214816065008
>500	0.002193130853877925	0.7922276214798055
>1k	9.398543431137568E-4	0.876819550988563
>5k	8.544130333615507E-5	0.2580861376136403
>10k+	8.544130333615507E-5	1.5785006274835662
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	41566	0.4067281282844353	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	37285	0.3648380470356823	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	26176	0.25613519429277243	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	3.914046367554591E-5	9.785115918886478E-6
10-11	0.0	0.0	0.0	4.892557959443239E-5	9.785115918886478E-6
12-13	0.0	0.0	0.0	5.381813755387563E-5	2.4462789797216194E-5
14-15	0.0	0.0	0.0	6.849581143220534E-5	5.8710695513318866E-5
16-17	0.0	0.0	0.0	7.338836939164859E-5	5.8710695513318866E-5
18-19	0.0	0.0	0.0	9.295860122942153E-5	6.849581143220534E-5
20-21	0.0	0.0	9.785115918886478E-6	1.516692967427404E-4	6.849581143220534E-5
22-23	0.0	0.0	9.785115918886478E-6	2.397353400127187E-4	7.828092735109182E-5
24-25	0.0	0.0	9.785115918886478E-6	4.011897526743456E-4	8.806604326997829E-5
26-27	0.0	0.0	9.785115918886478E-6	6.409250926870643E-4	9.785115918886478E-5
28-29	0.0	0.0	9.785115918886478E-6	0.001619436684575712	9.785115918886478E-5
30-31	0.0	0.0	9.785115918886478E-6	0.005367136081509233	9.785115918886478E-5
32-33	0.0	0.0	9.785115918886478E-6	0.013224584164375074	1.1742139102663773E-4
34-35	0.0	9.785115918886478E-6	9.785115918886478E-6	0.026439383212831263	1.272065069455242E-4
36-37	0.0	1.4677673878329716E-5	9.785115918886478E-6	0.04785900195927376	1.272065069455242E-4
38-39	0.0	1.9570231837772955E-5	9.785115918886478E-6	0.08014499193363969	1.3699162286441067E-4
40-41	0.0	1.9570231837772955E-5	9.785115918886478E-6	0.11777365519971764	1.3699162286441067E-4
42-43	0.0	2.9355347756659433E-5	9.785115918886478E-6	0.154247674787367	1.4677673878329717E-4
44-45	0.0	2.9355347756659433E-5	9.785115918886478E-6	0.19988545543305353	1.5656185470218364E-4
46-47	0.0	2.9355347756659433E-5	9.785115918886478E-6	0.25614987196665073	1.5656185470218364E-4
48-49	0.0	2.9355347756659433E-5	9.785115918886478E-6	0.31934704312877904	1.5656185470218364E-4
50-51	0.0	2.9355347756659433E-5	9.785115918886478E-6	0.3867664918099069	1.5656185470218364E-4
52-53	0.0	2.9355347756659433E-5	9.785115918886478E-6	0.45471433675065465	1.8591720245884305E-4
54-55	0.0	3.914046367554591E-5	9.785115918886478E-6	0.53155685206167	2.152725502155025E-4
56-57	0.0	4.892557959443239E-5	9.785115918886478E-6	0.6224850417379226	2.2016510817494573E-4
58-59	0.0	4.892557959443239E-5	9.785115918886478E-6	0.7296663089554457	2.3484278205327546E-4
60-61	0.0	5.381813755387563E-5	9.785115918886478E-6	0.8472687246265829	2.4462789797216193E-4
62-63	0.0	5.8710695513318866E-5	9.785115918886478E-6	0.9664563290765795	2.544130138910484E-4
64-65	0.0	5.8710695513318866E-5	9.785115918886478E-6	1.089802607792103	2.544130138910484E-4
66-67	0.0	5.8710695513318866E-5	9.785115918886478E-6	1.234093927132003	2.544130138910484E-4
68-69	0.0	5.8710695513318866E-5	9.785115918886478E-6	1.4016787149168124	2.544130138910484E-4
70-71	0.0	5.8710695513318866E-5	9.785115918886478E-6	1.5903455349488627	2.7398324572882135E-4
72-73	0.0	5.8710695513318866E-5	9.785115918886478E-6	1.779545643798492	3.326939412421402E-4
74-75	0.0	5.8710695513318866E-5	9.785115918886478E-6	1.9700716358551307	3.326939412421402E-4
76-77	0.0	6.36032534727621E-5	9.785115918886478E-6	2.179933016967489	3.3758649920158346E-4
78-79	0.0	6.849581143220534E-5	9.785115918886478E-6	2.4244581712225024	3.424790571610267E-4
80-81	0.0	6.849581143220534E-5	9.785115918886478E-6	2.689820729826785	3.914046367554591E-4
82-83	0.0	6.849581143220534E-5	9.785115918886478E-6	2.9745773881823	4.892557959443239E-4
84-85	0.0	6.849581143220534E-5	9.785115918886478E-6	3.2530030765382962	4.892557959443239E-4
86-87	0.0	6.849581143220534E-5	9.785115918886478E-6	3.541879268695663	4.941483539037671E-4
88	0.0	6.849581143220534E-5	9.785115918886478E-6	3.778776925091904	4.990409118632103E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	20315	0.0	48.75055	1
GTATCAA	33180	0.0	35.786808	1
TCAACGC	42060	0.0	27.830801	4
ATCAACG	42290	0.0	27.601929	3
CAACGCA	42535	0.0	27.486866	5
TACCTGG	8395	0.0	27.27945	2
TATCAAC	43815	0.0	26.831474	2
AACGCAG	44045	0.0	26.597614	6
GTACATG	40115	0.0	26.505243	1
TACATGG	40830	0.0	25.579763	2
ACATGGG	39970	0.0	25.171436	3
GTACCTG	9710	0.0	24.11838	1
CATGGGG	22210	0.0	23.950752	4
TATAACG	885	0.0	23.379583	2
ACGCAGA	50345	0.0	23.091711	7
CGCAGAG	51145	0.0	22.601887	8
ATGGGGG	13245	0.0	21.074427	5
ACCTGGG	10900	0.0	20.564465	3
GAGTACT	33590	0.0	20.361942	12-13
GCAGAGT	56845	0.0	20.029478	9
>>END_MODULE