FastQCFastQC Report
Mon 6 Jun 2016
ERR1211529_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211529_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6773526
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT238540.3521651795534556No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT209430.30918903979995055No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133140.1965593695218709No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA115500.047.5424041
GTATCAA196850.037.2253461
TACCTGG62650.037.152422
GTACCTG71450.033.3513071
TCAACGC234100.030.310274
ATCAACG236950.029.9873563
CAACGCA238600.029.8147535
AACGCAG247300.028.894396
ACCTGGG78750.028.763363
TATCAAC249700.028.7180082
GTACATG234650.027.5759661
TACATGG239000.026.3442382
ACATGGG237550.025.44073
ACGCAGA278600.025.3628257
CGCAGAG283350.024.7887888
CATGGGG133350.021.9890174
GCAGAGT314550.021.8833649
GAGTACT194150.020.33005312-13
ATGGGGG82800.019.4643365
CAGAGTA307150.019.39722810-11
Produced by FastQC (version 0.11.5)