FastQCFastQC Report
Mon 6 Jun 2016
ERR1211529_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211529_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6773526
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT267390.39475747195773664No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246190.3634591496363932No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT165710.24464363169197254No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130900.048.2086521
TACCTGG63200.035.862892
GTATCAA218900.035.7883261
GTACCTG71400.032.3366781
ACCTGGG76850.028.433653
TCAACGC270550.028.398184
ATCAACG273100.028.06443
CAACGCA273750.028.0314755
TATAACG6900.027.9415252
AACGCAG284500.027.071196
TATCAAC286600.026.9080982
GTACATG244750.026.667291
TAACGCA6950.026.369394
TACATGG250900.025.7514342
ACATGGG243400.025.4459293
ACGCAGA323250.023.6515547
CATGGGG129300.023.1505434
CGCAGAG330500.023.1185058
GCAGAGT365100.020.5538969
GAGTACT231900.020.06080412-13
Produced by FastQC (version 0.11.5)