##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211529_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6773526 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.125802425501874 33.0 31.0 34.0 30.0 34.0 2 32.34794832115504 34.0 31.0 34.0 31.0 34.0 3 32.361614024955394 34.0 31.0 34.0 30.0 34.0 4 35.99694058899309 37.0 35.0 37.0 35.0 37.0 5 35.921866395729495 37.0 35.0 37.0 35.0 37.0 6 35.94468863041199 37.0 35.0 37.0 35.0 37.0 7 35.86906051589674 37.0 35.0 37.0 35.0 37.0 8 35.86794086270577 37.0 35.0 37.0 35.0 37.0 9 37.52170051462119 39.0 37.0 39.0 35.0 39.0 10-11 37.489986456093916 39.0 37.0 39.0 35.0 39.0 12-13 37.40586594042748 39.0 37.0 39.0 34.0 39.0 14-15 38.64731448583795 40.0 38.0 41.0 34.0 41.0 16-17 38.577736248447266 40.0 38.0 41.0 34.0 41.0 18-19 38.570736053866185 40.0 38.0 41.0 34.0 41.0 20-21 38.512590709772134 40.0 38.0 41.0 34.0 41.0 22-23 38.40221008969331 40.0 38.0 41.0 34.0 41.0 24-25 38.29518067842361 40.0 38.0 41.0 34.0 41.0 26-27 38.121434537934896 40.0 38.0 41.0 33.0 41.0 28-29 38.154261384690926 40.0 38.0 41.0 33.5 41.0 30-31 38.073308790724354 40.0 37.5 41.0 33.0 41.0 32-33 37.88886792491827 40.0 37.0 41.0 33.0 41.0 34-35 37.77383795382198 40.0 37.0 41.0 33.0 41.0 36-37 37.71079722732296 40.0 37.0 41.0 33.0 41.0 38-39 37.61547700562454 40.0 37.0 41.0 32.5 41.0 40-41 37.4519086514173 40.0 37.0 41.0 32.0 41.0 42-43 37.29896622527174 40.0 36.0 41.0 31.0 41.0 44-45 37.11551199478676 40.0 36.0 41.0 31.0 41.0 46-47 36.856753926979835 39.0 36.0 41.0 30.0 41.0 48-49 36.63205766095827 39.0 35.0 41.0 30.0 41.0 50-51 36.43552516075084 39.0 35.0 41.0 29.5 41.0 52-53 36.2374081091591 39.0 35.0 40.0 28.5 41.0 54-55 36.043758007277155 39.0 35.0 40.0 28.0 41.0 56-57 35.847894582526145 38.0 35.0 40.0 28.0 41.0 58-59 35.59337802792814 38.0 34.0 40.0 27.5 41.0 60-61 35.28280913072453 38.0 34.0 40.0 26.5 41.0 62-63 34.9639197516921 37.5 34.0 40.0 26.0 41.0 64-65 34.649071473262225 37.0 33.0 40.0 26.0 41.0 66-67 34.30956572691977 36.5 33.0 39.0 26.0 41.0 68-69 33.91729292838029 36.0 32.5 39.0 25.0 40.0 70-71 33.530006897441595 35.5 32.0 38.5 24.5 40.0 72-73 33.123363739948736 35.0 32.0 38.0 24.0 40.0 74-75 32.712671878723135 35.0 31.0 37.0 23.0 39.0 76-77 31.170993954994785 33.5 29.5 35.5 21.5 38.5 78-79 31.99260776440513 34.0 31.0 36.0 22.5 39.0 80-81 31.955840946059702 34.0 31.0 36.0 23.0 37.5 82-83 31.668154296595304 34.0 31.0 35.5 22.0 37.0 84-85 31.36573255937897 34.0 30.5 35.0 20.5 37.0 86-87 31.066695912882004 34.0 30.0 35.0 20.0 36.0 88-89 30.78624736953841 34.0 30.0 35.0 20.0 36.0 90-91 30.499869418084465 34.0 29.5 35.0 19.0 35.5 92-93 30.290285296018645 34.0 29.0 35.0 19.0 35.0 94-95 30.06916323935274 34.0 29.0 35.0 18.0 35.0 96-97 29.86171205366304 34.0 29.0 35.0 18.0 35.0 98-99 29.695366342433765 34.0 29.0 35.0 18.0 35.0 100 27.7823756784871 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31492643019349487 1101 2 0.19910500656452257 1101 3 0.27414858191831115 1101 4 0.17108339056962762 1101 5 0.19956326525900892 1101 6 0.19807530785181626 1101 7 0.1953452364289987 1101 8 0.1460301260467176 1101 9 0.14531686310689196 1101 10-11 0.15999370963632487 1101 12-13 0.19986004699666182 1101 14-15 0.25467819007573667 1101 16-17 0.263020526811637 1101 18-19 0.2820383944671363 1101 20-21 0.26517569186565737 1101 22-23 0.2534474635470758 1101 24-25 0.26912062439897255 1101 26-27 0.22137847446727932 1101 28-29 0.33219940894581157 1101 30-31 0.28894551018922954 1101 32-33 0.3237211771285047 1101 34-35 0.30021759804569115 1101 36-37 0.3141331424479006 1101 38-39 0.30893439632404096 1101 40-41 0.31365837784687756 1101 42-43 0.3544901060055068 1101 44-45 0.2740905455235989 1101 46-47 0.3775041762920992 1101 48-49 0.35011748122030895 1101 50-51 0.2581017915494854 1101 52-53 0.35038288292656716 1101 54-55 0.22318168561268692 1101 56-57 0.28818738696932655 1101 58-59 0.347307361278439 1101 60-61 0.2736917296652379 1101 62-63 0.22397860629926214 1101 64-65 0.2084888766387536 1101 66-67 0.1295446587833311 1101 68-69 0.1892241025451682 1101 70-71 0.23809549085100912 1101 72-73 0.12092950897638133 1101 74-75 0.11766444481588678 1101 76-77 0.16473081236712517 1101 78-79 0.29338121216759205 1101 80-81 0.2539092473291227 1101 82-83 0.2084661203520355 1101 84-85 0.16527901313610371 1101 86-87 0.20641528499836426 1101 88-89 0.09029417717970745 1101 90-91 0.07536750322559982 1101 92-93 0.04941167555164938 1101 94-95 0.10404569838772915 1101 96-97 0.09208517251870418 1101 98-99 -0.05688125327462501 1101 100 -0.10731183761187069 1102 1 0.3238196310649357 1102 2 0.2633180426475974 1102 3 0.3316608133841754 1102 4 0.20079224544958407 1102 5 0.19598913440091792 1102 6 0.15649271058152436 1102 7 0.15367155095936624 1102 8 0.15899391268345653 1102 9 0.18970202377170864 1102 10-11 0.19361620838208893 1102 12-13 0.24386815239315496 1102 14-15 0.33939928480913295 1102 16-17 0.3176544824147456 1102 18-19 0.23156077527907115 1102 20-21 0.2711325407464571 1102 22-23 0.23958661872188713 1102 24-25 0.24052431220977155 1102 26-27 0.2557856737398154 1102 28-29 0.29320802604735263 1102 30-31 0.3392840391142755 1102 32-33 0.40304758805771 1102 34-35 0.3615196422267246 1102 36-37 0.448925153031432 1102 38-39 0.40345873067912663 1102 40-41 0.4099747473830746 1102 42-43 0.2652972750145963 1102 44-45 0.3758537992019768 1102 46-47 0.4290107943063859 1102 48-49 0.44221920497612643 1102 50-51 0.44592525490438817 1102 52-53 0.39323317065278474 1102 54-55 0.4458480986126858 1102 56-57 0.43251880972304946 1102 58-59 0.43825519651812783 1102 60-61 0.4695930226386409 1102 62-63 0.511792449505954 1102 64-65 0.45983145874645004 1102 66-67 0.37824761180462474 1102 68-69 0.3703437471371984 1102 70-71 0.28490740656538094 1102 72-73 0.3545125475319466 1102 74-75 0.26592443863293624 1102 76-77 0.27230445077122667 1102 78-79 0.28319453015939544 1102 80-81 0.38142464382734076 1102 82-83 0.32435941076871444 1102 84-85 0.3098852882967087 1102 86-87 0.3221236360495183 1102 88-89 0.2566194170953864 1102 90-91 0.27849053668618495 1102 92-93 0.23397161755231366 1102 94-95 0.3533093094128077 1102 96-97 0.36042256636673287 1102 98-99 0.2997608750767746 1102 100 0.3511081397005853 1103 1 0.2513886569896897 1103 2 0.22437118767858522 1103 3 0.2786941062034387 1103 4 0.12073202960358032 1103 5 0.15046770113465158 1103 6 0.1610536294368785 1103 7 0.14698660601451508 1103 8 0.1787713803257347 1103 9 0.19291338200306996 1103 10-11 0.21003364721967444 1103 12-13 0.21552387153938213 1103 14-15 0.3348765643832792 1103 16-17 0.330385443130659 1103 18-19 0.2829404234301265 1103 20-21 0.23116366459823467 1103 22-23 0.25972228125742447 1103 24-25 0.3158176893352973 1103 26-27 0.25521876333112914 1103 28-29 0.35588444872478675 1103 30-31 0.4444353131589338 1103 32-33 0.42176713063446414 1103 34-35 0.42690072470463036 1103 36-37 0.4107735287040981 1103 38-39 0.38834804736698203 1103 40-41 0.3756055412144903 1103 42-43 0.4432363641593682 1103 44-45 0.4269613110158872 1103 46-47 0.5238366064788451 1103 48-49 0.44965637326298236 1103 50-51 0.4266859235719025 1103 52-53 0.46398620068935514 1103 54-55 0.4840896760655795 1103 56-57 0.5788321663239273 1103 58-59 0.5597620094827604 1103 60-61 0.5478827039144605 1103 62-63 0.5963740934489081 1103 64-65 0.6441653430163115 1103 66-67 0.5968027965174585 1103 68-69 0.5266744814300495 1103 70-71 0.5529470610539491 1103 72-73 0.5629103698364091 1103 74-75 0.5413290580248429 1103 76-77 0.60797565106839 1103 78-79 0.4812181777037843 1103 80-81 0.4560559881029498 1103 82-83 0.44878203425408714 1103 84-85 0.3009149607664092 1103 86-87 0.4365786321104679 1103 88-89 0.4930595284811261 1103 90-91 0.4569122447455314 1103 92-93 0.43233854106363623 1103 94-95 0.5185785904942719 1103 96-97 0.556390479052979 1103 98-99 0.49988562965296524 1103 100 0.5824772842945372 1104 1 0.24658792966270937 1104 2 0.18875145550028094 1104 3 0.22365055712307935 1104 4 0.0713480792309582 1104 5 0.14780266450515 1104 6 0.11128353664624768 1104 7 0.15290061389549692 1104 8 0.16414386700352424 1104 9 0.15248031569271348 1104 10-11 0.15656286264241714 1104 12-13 0.22582967241473284 1104 14-15 0.31583085546520806 1104 16-17 0.2823863616614304 1104 18-19 0.25468912402871524 1104 20-21 0.21134733193496658 1104 22-23 0.33314044982121516 1104 24-25 0.2961665215552287 1104 26-27 0.295332034687938 1104 28-29 0.3582521326119945 1104 30-31 0.3191262497263452 1104 32-33 0.3340454051716506 1104 34-35 0.47191100545800424 1104 36-37 0.28768996852695494 1104 38-39 0.3814344999403758 1104 40-41 0.4431288918675804 1104 42-43 0.5019549368018588 1104 44-45 0.39321625096884816 1104 46-47 0.4126678846581555 1104 48-49 0.45343872951995934 1104 50-51 0.48539415373837613 1104 52-53 0.4693666924260995 1104 54-55 0.515737565237032 1104 56-57 0.5471221436131373 1104 58-59 0.5450348744241467 1104 60-61 0.48455173667385765 1104 62-63 0.4830065507369312 1104 64-65 0.5282807668670628 1104 66-67 0.509258396871175 1104 68-69 0.49966742648008733 1104 70-71 0.388990909934968 1104 72-73 0.49019322531238174 1104 74-75 0.5732184435740635 1104 76-77 0.5184792249140386 1104 78-79 0.627912884982333 1104 80-81 0.6805483386398414 1104 82-83 0.4744918472128177 1104 84-85 0.5096396252409257 1104 86-87 0.681877474064386 1104 88-89 0.616536898321808 1104 90-91 0.48417537870215455 1104 92-93 0.6046094571433329 1104 94-95 0.6001414545463675 1104 96-97 0.5148055948815404 1104 98-99 0.5773209365475367 1104 100 0.677427124072711 1105 1 0.2054034249591794 1105 2 0.14721058989331226 1105 3 0.16627593271439167 1105 4 0.10626131920169968 1105 5 0.11781326782573842 1105 6 0.10759380369663774 1105 7 0.1482452670888179 1105 8 0.11070207903311058 1105 9 0.08497760804596055 1105 10-11 0.18271489267465313 1105 12-13 0.20408860620847236 1105 14-15 0.29916281416477375 1105 16-17 0.2823199086025241 1105 18-19 0.2848297870894143 1105 20-21 0.22107094357917134 1105 22-23 0.2946739530698963 1105 24-25 0.2822319763240131 1105 26-27 0.2948660222109396 1105 28-29 0.3759028416984336 1105 30-31 0.37109662184433745 1105 32-33 0.39186862128102007 1105 34-35 0.45201129830778086 1105 36-37 0.4452519964670856 1105 38-39 0.44103696158286 1105 40-41 0.4267827546603229 1105 42-43 0.3982035037036624 1105 44-45 0.4680023462939431 1105 46-47 0.5284661558979806 1105 48-49 0.5297103598474777 1105 50-51 0.6329943160889187 1105 52-53 0.516109086888271 1105 54-55 0.5672772943329605 1105 56-57 0.5680632499185094 1105 58-59 0.6106267188595353 1105 60-61 0.5796201877982767 1105 62-63 0.582446283538907 1105 64-65 0.6479560600599825 1105 66-67 0.6324600596167258 1105 68-69 0.6373671299117234 1105 70-71 0.6930841338327838 1105 72-73 0.6278679294378904 1105 74-75 0.7225879734212199 1105 76-77 0.6982862062914883 1105 78-79 0.7641924775083204 1105 80-81 0.7182015258249983 1105 82-83 0.774799743104964 1105 84-85 0.6789721568308202 1105 86-87 0.6865985312877285 1105 88-89 0.6802088873115224 1105 90-91 0.7314343533143699 1105 92-93 0.7787724112102303 1105 94-95 0.8623854015692416 1105 96-97 0.9106918597420126 1105 98-99 0.8488822699113889 1105 100 0.8717917722550759 1106 1 0.21446464464942494 1106 2 0.15500157432153117 1106 3 0.19804919698913181 1106 4 0.15831494563600046 1106 5 0.1324457966724779 1106 6 0.14329919719805417 1106 7 0.17970545973760466 1106 8 0.1825276594645473 1106 9 0.2028223072828368 1106 10-11 0.1582448375210248 1106 12-13 0.18049232508892032 1106 14-15 0.24567122378793727 1106 16-17 0.28367337826365 1106 18-19 0.2788207053464262 1106 20-21 0.29326091301698654 1106 22-23 0.27850886868152 1106 24-25 0.2921010635958794 1106 26-27 0.2787602618464007 1106 28-29 0.3076479777310368 1106 30-31 0.34041834137553906 1106 32-33 0.4116626323482677 1106 34-35 0.3997359873633499 1106 36-37 0.38903573711976236 1106 38-39 0.34040441849396785 1106 40-41 0.37595923897515604 1106 42-43 0.38583336744282093 1106 44-45 0.5088769273830707 1106 46-47 0.5380606801207719 1106 48-49 0.5381671281726454 1106 50-51 0.5699408671336883 1106 52-53 0.6238555189563257 1106 54-55 0.5203571040432564 1106 56-57 0.5633038801372123 1106 58-59 0.6292632358728767 1106 60-61 0.6780289929514467 1106 62-63 0.6348984253571004 1106 64-65 0.6912441830946108 1106 66-67 0.6662218472765247 1106 68-69 0.6198529763207432 1106 70-71 0.6184243933539335 1106 72-73 0.6299554083622567 1106 74-75 0.6071033715568177 1106 76-77 0.5453181061465457 1106 78-79 0.6112139422511511 1106 80-81 0.674875295915097 1106 82-83 0.702096088606325 1106 84-85 0.6503275637892436 1106 86-87 0.600764383291363 1106 88-89 0.6439591607425008 1106 90-91 0.6245447872945249 1106 92-93 0.7049066704779463 1106 94-95 0.7204283807198308 1106 96-97 0.7412164436978337 1106 98-99 0.7622869064974189 1106 100 0.9156789510718184 1107 1 0.17616654541539845 1107 2 0.13699173402411446 1107 3 0.1885775430028218 1107 4 0.11429947180509004 1107 5 0.11247433206408175 1107 6 0.13006333527111025 1107 7 0.13347573512619704 1107 8 0.11530824216018232 1107 9 0.18181956038367275 1107 10-11 0.12372289726148722 1107 12-13 0.15140663433754753 1107 14-15 0.1875344083523558 1107 16-17 0.16680173604450488 1107 18-19 0.17893184996128753 1107 20-21 0.17496912025158906 1107 22-23 0.21148645957372025 1107 24-25 0.23886555100684603 1107 26-27 0.2997676972280843 1107 28-29 0.24155682995374406 1107 30-31 0.19175207675390737 1107 32-33 0.20113174903816855 1107 34-35 0.23624896986336097 1107 36-37 0.36460469826236164 1107 38-39 0.28683703488784573 1107 40-41 0.28352929961286577 1107 42-43 0.24520601171018797 1107 44-45 0.32585291132087946 1107 46-47 0.3559847487675043 1107 48-49 0.37700559799904454 1107 50-51 0.41342838106124447 1107 52-53 0.3951610866672084 1107 54-55 0.39073214587187977 1107 56-57 0.46808787898991255 1107 58-59 0.560639204366268 1107 60-61 0.5475676462721779 1107 62-63 0.463579527646921 1107 64-65 0.5793721179927331 1107 66-67 0.559706235549065 1107 68-69 0.46329571983685014 1107 70-71 0.586205968904693 1107 72-73 0.5082140875707708 1107 74-75 0.6083916010754962 1107 76-77 0.4543350692601358 1107 78-79 0.549329558391296 1107 80-81 0.5614449998604094 1107 82-83 0.5673208783600643 1107 84-85 0.47481357740239005 1107 86-87 0.47845097570227324 1107 88-89 0.4213416453072796 1107 90-91 0.33031198850948584 1107 92-93 0.4790287291446518 1107 94-95 0.5280057039088817 1107 96-97 0.5694349490463786 1107 98-99 0.6720760902601945 1107 100 0.8907766737563492 1108 1 0.22205001442147676 1108 2 0.16732177567459416 1108 3 0.2138630882084982 1108 4 0.12542656449321754 1108 5 0.11090173283927385 1108 6 0.10839478635212174 1108 7 0.1570598447003917 1108 8 0.10568201229651919 1108 9 0.1502447024560709 1108 10-11 0.15669363350824028 1108 12-13 0.16571786551126166 1108 14-15 0.20970149814934302 1108 16-17 0.2468919034595558 1108 18-19 0.2292829427216887 1108 20-21 0.27056076485825287 1108 22-23 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2316 78-79 -0.38693938604831146 2316 80-81 -0.25774639738892446 2316 82-83 -0.160100676892327 2316 84-85 -0.20471644218172358 2316 86-87 -0.26467737288806603 2316 88-89 -0.2112350093089006 2316 90-91 -0.13304532440740502 2316 92-93 -0.05637453150117011 2316 94-95 -0.07763503089598345 2316 96-97 -0.027073374681446438 2316 98-99 -0.17796952550860112 2316 100 -0.2599870837103566 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 1.0 10 10.0 11 23.0 12 77.0 13 207.0 14 681.0 15 1706.0 16 3812.0 17 8234.0 18 14628.0 19 22561.0 20 31528.0 21 43263.0 22 56382.0 23 64685.0 24 68584.0 25 73974.0 26 85410.0 27 101146.0 28 122678.0 29 147760.0 30 179074.0 31 216508.0 32 266715.0 33 336521.0 34 442979.0 35 608474.0 36 892561.0 37 1380081.0 38 1379585.0 39 223650.0 40 27.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.528427457663504 20.199805323643904 13.617881287580502 23.65388593111209 2 15.195784198248221 21.397149863443822 39.970887909884596 23.436178028423363 3 17.241223020796763 27.131252603913687 30.985177733441322 24.642346641848224 4 11.813798808262058 17.529274881157647 38.52035050282211 32.136575807758184 5 12.166519818065211 38.5212424572771 35.80197938529096 13.510258339366723 6 30.368106311115206 38.320593940070374 18.018881880180317 13.292417868634104 7 26.596221820068305 33.17896764550694 23.00327776109518 17.221532773329578 8 25.62755602418203 36.49941632480539 20.437548968702696 17.435478682309878 9 26.472641645229032 15.216419810955154 20.648023001317632 37.66291554249818 10-11 24.2766691971065 26.68116280944371 29.31947851089669 19.722689482553104 12-13 24.82548232633934 24.54136885279543 29.287309150359796 21.345839670505434 14-15 22.23226868842018 25.971731709599993 26.79740212114045 24.998597480839376 16-17 21.645219258436004 28.862718477447597 27.55149208647623 21.94057017764017 18-19 21.477673808219347 27.824491983930805 29.646390314428807 21.05144389342104 20-21 22.20483697504475 27.15922887554057 29.663922486272764 20.97201166314192 22-23 22.004381469857798 27.01642394227172 29.432669188839018 21.546525399031466 24-25 21.75416948739168 27.177077457976274 29.5529675872583 21.515785467373743 26-27 21.70910524299422 27.258373237528815 29.571219669551184 21.461301849925775 28-29 21.68611082301764 27.22319435827271 29.698407349569994 21.392287469139653 30-31 22.040916026116538 27.14691175839878 29.60159134037297 21.210580875111713 32-33 21.792893160747376 27.327970084982688 29.538318761086234 21.3408179931837 34-35 21.993679249210793 27.401293264706467 29.47154879654452 21.133478689538222 36-37 22.002912704278874 27.27907963328183 29.32979086446668 21.38821679797261 38-39 21.851824648599248 27.502575320987788 29.323750789389862 21.3218492410231 40-41 21.943269012288063 27.328289502726864 29.4384053290348 21.290036155950272 42-43 21.818117391721604 27.399627937192257 29.237920877595762 21.544333793490377 44-45 21.750244642231774 27.59797648245515 29.13708173855733 21.514697136755746 46-47 21.808237574960835 27.511498903667587 28.89299881279255 21.78726470857903 48-49 21.779800944383933 27.642512839070992 28.90093595325239 21.676750263292682 50-51 21.84912765812313 27.789861031832956 28.684372083051947 21.67663922699197 52-53 21.89680314425175 27.87340314817896 28.502170819351246 21.727622888218047 54-55 21.79026542969602 27.833707137600744 28.640225246629246 21.73580218607399 56-57 21.879983928152374 27.889830333936704 28.425319907056117 21.8048658308548 58-59 21.822739561642734 27.764920080173766 28.668927114866516 21.743413243316983 60-61 22.023296282363003 27.95333154584696 28.51336303552984 21.510009136260205 62-63 21.790617635259473 27.951363126216584 28.647363174647328 21.61065606387661 64-65 21.763952140047145 27.671482366989498 28.625195659048767 21.93936983391459 66-67 21.70757751169122 27.797510253119196 28.433538068428543 22.061374166761038 68-69 21.642825771894174 27.888335740198265 28.51259733739044 21.956241150517123 70-71 21.730042404367968 27.668255840031254 28.52390987141382 22.077791884186954 72-73 21.647269174394353 27.925575050042468 28.27137467568828 22.155781099874908 74-75 21.615763961349696 28.013231369211226 28.19635487736955 22.17464979206952 76-77 21.548768259958607 27.881884294780207 28.356562571304533 22.21278487395665 78-79 21.55345032203045 27.811960261994678 28.331097974564155 22.303491441410713 80-81 21.53599510060524 28.031701670797847 28.061658493257465 22.370644735339447 82-83 21.694870480892092 28.002734062175406 28.084205157729443 22.218190299203062 84-85 21.564556952305914 28.045664498080196 28.01745402268385 22.37232452693004 86-87 21.426094296268126 27.9752407857508 28.221351817244194 22.377313100736888 88-89 21.404068917719332 27.858971435001223 28.18326040732296 22.55369923995649 90-91 21.616021910779907 27.917098880191332 28.08634420861196 22.380535000416803 92-93 21.50437216083503 27.91353209955728 28.031880022513207 22.550215717094485 94-95 21.322933814983422 27.743189762114746 28.085921637633927 22.847954785267905 96-97 21.341282701286765 27.70666409921052 28.17401150600352 22.778041693499194 98-99 21.25779549416395 27.644635399858675 28.099933825650414 22.997635280326964 100 20.39780344432617 28.08698866061874 28.299207987195963 23.21599990785913 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3069.0 1 2260.5 2 2686.0 3 3849.5 4 2822.0 5 2233.5 6 3407.0 7 6444.5 8 10131.0 9 11833.0 10 9996.5 11 7692.0 12 7720.5 13 8176.5 14 8706.0 15 9195.5 16 9590.0 17 10188.5 18 10915.5 19 11835.0 20 13096.5 21 14953.5 22 17898.5 23 22193.5 24 27525.5 25 33790.0 26 42028.5 27 52094.0 28 62498.0 29 74248.5 30 88422.5 31 102284.0 32 117711.5 33 135774.0 34 153305.0 35 168693.0 36 188268.5 37 206713.0 38 216754.0 39 225412.0 40 228199.0 41 227374.5 42 228552.5 43 231554.5 44 236502.0 45 243164.0 46 245957.5 47 245839.5 48 248006.0 49 248780.5 50 247681.0 51 239729.5 52 225782.0 53 214113.0 54 202777.5 55 186621.0 56 168232.5 57 150056.0 58 132050.0 59 113201.0 60 93350.5 61 75719.0 62 60544.0 63 46195.5 64 33720.0 65 24640.0 66 18213.0 67 13597.5 68 10070.5 69 7362.0 70 5471.5 71 4132.0 72 3188.0 73 2378.5 74 1747.5 75 1283.0 76 907.0 77 610.0 78 405.0 79 268.5 80 185.0 81 127.0 82 96.5 83 76.0 84 46.0 85 31.0 86 24.0 87 17.5 88 14.0 89 7.5 90 8.5 91 7.0 92 3.0 93 3.0 94 3.0 95 3.0 96 3.0 97 4.5 98 3.5 99 2.5 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0034398627834306686 2 0.06668609524788123 3 0.0010039084518166756 4 8.415114963757428E-4 5 0.0016239695544093284 6 7.086412601058887E-4 7 0.0 8 9.891450922311364E-4 9 1.4763359585539348E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 8.562748559612822E-4 18-19 0.001018671811402215 20-21 0.003284847507782505 22-23 0.0 24-25 2.2145039378309023E-5 26-27 1.6239695544093284E-4 28-29 0.0010112901316094453 30-31 0.0017199313917153343 32-33 0.0030338703948283364 34-35 0.004133740683951018 36-37 0.0014099008404190078 38-39 0.0014541909191756258 40-41 0.005418152967892941 42-43 0.007027359162716729 44-45 0.005462443046649559 46-47 0.007219282837328741 48-49 0.00952236693267288 50-51 0.008976122628007923 52-53 0.0040894506051943995 54-55 0.002221885617623672 56-57 0.0028271833606307853 58-59 0.0035136795813583647 60-61 0.007588366826967226 62-63 0.014305695438387628 64-65 0.003897526930582388 66-67 0.010467221946147399 68-69 0.013058191553409553 70-71 0.005949633912972357 72-73 0.007802435540957545 74-75 0.0033734276652957413 76-77 0.0029748169564861784 78-79 0.008326534806244193 80-81 0.00656231333577224 82-83 0.017501962788656896 84-85 0.017996535334772464 86-87 0.02336301654411602 88-89 0.021842390506805465 90-91 0.02509032961562412 92-93 0.0352696660498535 94-95 0.03890883418768895 96-97 0.023370398223908788 98-99 0.018668268195914505 100 0.01900044378658914 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6773526.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.058965060636545 #Duplication Level Percentage of deduplicated Percentage of total 1 73.87538962382463 43.63004054634371 2 16.264128927879877 19.21085244186692 3 4.97267807328254 8.810436617633611 4 1.928016705774208 4.554666850505701 5 0.9422468103507735 2.7824060725501285 6 0.5412468272846965 1.9179286477072368 7 0.3229580491582282 1.3351497698900996 8 0.21100951673410248 0.9969602941008929 9 0.15345496478216114 0.8156602263105781 >10 0.7111369045204182 7.327918481342562 >50 0.043917399487643165 1.7835549765163723 >100 0.030556393425544233 3.4951268566511864 >500 0.0020680489204732604 0.8156252607835212 >1k 0.0010515481541097651 1.0066538314516573 >5k 3.505160513662464E-5 0.11081306983064065 >10k+ 1.0515481540987393E-4 1.4062060565151484 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26739 0.39475747195773664 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24619 0.3634591496363932 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16571 0.24464363169197254 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.4290078756618046E-5 2 0.0 0.0 0.0 0.0 4.4290078756618046E-5 3 0.0 0.0 0.0 0.0 4.4290078756618046E-5 4 0.0 0.0 0.0 0.0 4.4290078756618046E-5 5 0.0 0.0 0.0 0.0 4.4290078756618046E-5 6 1.4763359585539348E-5 0.0 0.0 0.0 4.4290078756618046E-5 7 1.4763359585539348E-5 0.0 0.0 0.0 4.4290078756618046E-5 8 1.4763359585539348E-5 0.0 0.0 0.0 4.4290078756618046E-5 9 1.4763359585539348E-5 0.0 0.0 0.0 4.4290078756618046E-5 10-11 1.4763359585539348E-5 0.0 0.0 1.4763359585539348E-5 4.4290078756618046E-5 12-13 1.4763359585539348E-5 0.0 0.0 1.4763359585539348E-5 4.4290078756618046E-5 14-15 1.4763359585539348E-5 0.0 0.0 1.4763359585539348E-5 4.4290078756618046E-5 16-17 2.2145039378309023E-5 0.0 0.0 1.4763359585539348E-5 4.4290078756618046E-5 18-19 2.9526719171078695E-5 0.0 0.0 2.9526719171078695E-5 4.4290078756618046E-5 20-21 2.9526719171078695E-5 0.0 0.0 5.90534383421574E-5 4.4290078756618046E-5 22-23 4.4290078756618046E-5 0.0 0.0 1.697786352337025E-4 6.643511813492708E-5 24-25 5.167175854938772E-5 0.0 0.0 3.247939108818657E-4 8.858015751323609E-5 26-27 7.381679792769675E-5 0.0 0.0 6.12679422799883E-4 8.858015751323609E-5 28-29 7.381679792769675E-5 0.0 0.0 0.0016682596331659464 8.858015751323609E-5 30-31 7.381679792769675E-5 0.0 0.0 0.005816763676702504 9.596183730600576E-5 32-33 7.381679792769675E-5 0.0 0.0 0.014556672551341798 1.1810687668431478E-4 34-35 7.381679792769675E-5 0.0 0.0 0.028279215286100622 1.3287023626985413E-4 36-37 8.858015751323609E-5 0.0 0.0 0.05059403329964335 1.3287023626985413E-4 38-39 1.0334351709877544E-4 0.0 0.0 0.08663877572773766 1.3287023626985413E-4 40-41 1.0334351709877544E-4 0.0 0.0 0.12856671695066943 1.3287023626985413E-4 42-43 1.476335958553935E-4 0.0 0.0 0.17018462762230485 1.3287023626985413E-4 44-45 1.6239695544093284E-4 0.0 0.0 0.21961235551469058 1.6239695544093284E-4 46-47 1.6239695544093284E-4 0.0 0.0 0.28054812220400427 1.6239695544093284E-4 48-49 1.7716031502647218E-4 0.0 0.0 0.34943395803013083 1.9192367461201153E-4 50-51 1.993053544047812E-4 0.0 0.0 0.4208812367443485 1.9192367461201153E-4 52-53 2.2145039378309022E-4 0.0 0.0 0.4940558285300743 2.2145039378309022E-4 54-55 2.288320735758599E-4 7.381679792769674E-6 0.0 0.5796094973282748 2.583587927469386E-4 56-57 2.3621375336862956E-4 2.9526719171078695E-5 0.0 0.6819786326944046 2.6574047253970825E-4 58-59 2.3621375336862956E-4 2.9526719171078695E-5 0.0 0.7960477305320743 2.6574047253970825E-4 60-61 2.3621375336862956E-4 2.9526719171078695E-5 0.0 0.9204511800796218 2.6574047253970825E-4 62-63 2.509771129541689E-4 2.9526719171078695E-5 0.0 1.0487078664789948 2.805038321252476E-4 64-65 2.6574047253970825E-4 2.9526719171078695E-5 0.0 1.1792454919343336 2.878855119180173E-4 66-67 2.6574047253970825E-4 2.9526719171078695E-5 0.0 1.3321274621223864 3.026488715035567E-4 68-69 2.6574047253970825E-4 2.9526719171078695E-5 0.0 1.512225685706381 3.1003055129632634E-4 70-71 2.6574047253970825E-4 3.6908398963848374E-5 0.0 1.7114646049930273 3.39557270467405E-4 72-73 2.878855119180173E-4 4.4290078756618046E-5 0.0 1.9102532418123146 4.1337406839510175E-4 74-75 2.95267191710787E-4 4.4290078756618046E-5 0.0 2.1115590314409363 4.281374279806411E-4 76-77 2.95267191710787E-4 4.4290078756618046E-5 0.0 2.333991188636465 4.281374279806411E-4 78-79 2.95267191710787E-4 4.4290078756618046E-5 0.0 2.5918701721968738 4.281374279806411E-4 80-81 2.95267191710787E-4 4.4290078756618046E-5 0.0 2.8705581110931 4.650458269444895E-4 82-83 2.95267191710787E-4 4.4290078756618046E-5 0.0 3.1588200886805486 5.019542259083378E-4 84-85 3.247939108818657E-4 4.4290078756618046E-5 0.0 3.451400348946767 5.019542259083378E-4 86-87 3.247939108818657E-4 4.4290078756618046E-5 0.0 3.756344922865875 5.019542259083378E-4 88 3.247939108818657E-4 4.4290078756618046E-5 0.0 4.006775791515379 5.019542259083378E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13090 0.0 48.208652 1 TACCTGG 6320 0.0 35.86289 2 GTATCAA 21890 0.0 35.788326 1 GTACCTG 7140 0.0 32.336678 1 ACCTGGG 7685 0.0 28.43365 3 TCAACGC 27055 0.0 28.39818 4 ATCAACG 27310 0.0 28.0644 3 CAACGCA 27375 0.0 28.031475 5 TATAACG 690 0.0 27.941525 2 AACGCAG 28450 0.0 27.07119 6 TATCAAC 28660 0.0 26.908098 2 GTACATG 24475 0.0 26.66729 1 TAACGCA 695 0.0 26.36939 4 TACATGG 25090 0.0 25.751434 2 ACATGGG 24340 0.0 25.445929 3 ACGCAGA 32325 0.0 23.651554 7 CATGGGG 12930 0.0 23.150543 4 CGCAGAG 33050 0.0 23.118505 8 GCAGAGT 36510 0.0 20.553896 9 GAGTACT 23190 0.0 20.060804 12-13 >>END_MODULE