FastQCFastQC Report
Mon 6 Jun 2016
ERR1211528_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211528_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6665085
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT221860.33286897316388314No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT201480.302291718710264No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT128630.19299078706423098No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA109450.050.332541
GTATCAA182200.036.6184731
TCAACGC215400.030.2583314
ATCAACG217050.030.008463
CAACGCA217800.029.9224345
AACGCAG226850.028.701966
TACCTGG58750.028.492692
TATCAAC232050.028.3685912
GTACATG209250.027.0919191
GTACCTG66250.026.2962971
TACATGG209350.026.0541152
ACGCAGA258000.025.0918627
ACATGGG209650.024.6792933
CGCAGAG265300.024.2778438
CATGGGG124450.023.259484
ACCTGGG72700.021.9778273
GCAGAGT295950.021.1306069
ATGGGGG81800.021.0235525
GAGTACT190550.020.68791212-13
CAGAGTA289600.019.54936410-11
Produced by FastQC (version 0.11.5)