FastQCFastQC Report
Mon 6 Jun 2016
ERR1211528_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211528_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6665085
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT240110.3602504694238708No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT223530.3353745676161669No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152410.22866925177998482No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA117300.049.907641
GTATCAA188800.036.287181
TACCTGG57900.029.2358932
TCAACGC235800.028.4776294
ATCAACG237150.028.2560713
CAACGCA238600.028.1237455
AACGCAG246750.027.2896466
TATCAAC248350.027.2640742
GTACCTG65300.026.7870861
GTACATG211400.025.9351791
TACATGG214300.024.9915942
ACATGGG212550.024.3411773
ACGCAGA282100.023.7531977
CGCAGAG286450.023.2284418
CATGGGG121850.022.945994
ACCTGGG72500.022.7501623
GCAGAGT321850.020.2646149
GAGTACT206700.019.9152412-13
ATGGGGG77750.019.8843275
AGAGTAC296300.019.34040510-11
Produced by FastQC (version 0.11.5)