##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211528_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	6665085
Sequences flagged as poor quality	0
Sequence length	100
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.13068475495811	33.0	31.0	34.0	30.0	34.0
2	32.35183452274052	34.0	31.0	34.0	31.0	34.0
3	32.36663478410253	34.0	31.0	34.0	30.0	34.0
4	35.99718443200649	37.0	35.0	37.0	35.0	37.0
5	35.92309145344733	37.0	35.0	37.0	35.0	37.0
6	35.94210606466384	37.0	35.0	37.0	35.0	37.0
7	35.868365819790746	37.0	35.0	37.0	35.0	37.0
8	35.86574814874829	37.0	35.0	37.0	35.0	37.0
9	37.520520743546406	39.0	37.0	39.0	35.0	39.0
10-11	37.48776467216847	39.0	37.0	39.0	35.0	39.0
12-13	37.40186561761778	39.0	37.0	39.0	34.0	39.0
14-15	38.648774921850205	40.0	38.0	41.0	34.0	41.0
16-17	38.57660667493363	40.0	38.0	41.0	34.0	41.0
18-19	38.56459467508667	40.0	38.0	41.0	34.0	41.0
20-21	38.504599341193696	40.0	38.0	41.0	34.0	41.0
22-23	38.396589690904165	40.0	38.0	41.0	34.0	41.0
24-25	38.289861119550615	40.0	38.0	41.0	34.0	41.0
26-27	38.11473552100236	40.0	38.0	41.0	33.0	41.0
28-29	38.15249565459405	40.0	38.0	41.0	33.5	41.0
30-31	38.07680667238303	40.0	37.5	41.0	33.0	41.0
32-33	37.89342776573742	40.0	37.0	41.0	33.0	41.0
34-35	37.77854791049177	40.0	37.0	41.0	33.0	41.0
36-37	37.72012584985788	40.0	37.0	41.0	33.0	41.0
38-39	37.62505819505678	40.0	37.0	41.0	32.5	41.0
40-41	37.46412243804843	40.0	37.0	41.0	32.0	41.0
42-43	37.312013050096134	40.0	36.0	41.0	31.0	41.0
44-45	37.131526979775956	40.0	36.0	41.0	31.0	41.0
46-47	36.88042448070805	39.0	36.0	41.0	30.0	41.0
48-49	36.658070902321576	39.0	35.5	41.0	30.0	41.0
50-51	36.460881369104825	39.0	35.0	41.0	29.5	41.0
52-53	36.26379378507551	39.0	35.0	40.0	29.0	41.0
54-55	36.07002813917602	39.0	35.0	40.0	28.0	41.0
56-57	35.87293867970176	38.0	35.0	40.0	28.0	41.0
58-59	35.62032179634618	38.0	34.0	40.0	27.5	41.0
60-61	35.30848541316428	38.0	34.0	40.0	26.5	41.0
62-63	34.98758763016526	37.5	34.0	40.0	26.0	41.0
64-65	34.673366581221394	37.0	33.0	40.0	26.0	41.0
66-67	34.335951379464774	36.5	33.0	39.0	26.0	41.0
68-69	33.94098214801461	36.0	32.5	39.0	25.5	40.0
70-71	33.554789848891645	35.5	32.0	39.0	25.0	40.0
72-73	33.147048012140885	35.0	32.0	38.0	24.0	40.0
74-75	32.739797692002426	35.0	31.0	37.0	23.0	39.0
76-77	31.18355467334625	33.5	29.5	35.5	21.5	38.5
78-79	32.01375818913037	34.0	31.0	36.0	22.5	39.0
80-81	31.982297300034432	34.5	31.0	36.0	23.0	37.5
82-83	31.694781461901837	34.0	31.0	35.5	22.0	37.0
84-85	31.393524913785797	34.0	31.0	35.0	20.5	37.0
86-87	31.09489203813605	34.0	30.0	35.0	20.0	36.0
88-89	30.81450851714569	34.0	30.0	35.0	20.0	36.0
90-91	30.529274345338433	34.0	29.5	35.0	19.5	35.5
92-93	30.3179826663876	34.0	29.0	35.0	19.0	35.0
94-95	30.092856280152468	34.0	29.0	35.0	18.0	35.0
96-97	29.888425578968608	34.0	29.0	35.0	18.0	35.0
98-99	29.721755986607825	34.0	29.0	35.0	18.0	35.0
100	27.83255187293185	31.0	25.0	34.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.26013181653299
1101	2	0.20062486689586478
1101	3	0.2513678704398643
1101	4	0.14034026760005247
1101	5	0.12812474322185352
1101	6	0.14840919228716132
1101	7	0.1996179134611893
1101	8	0.17379433507683473
1101	9	0.16244970633238864
1101	10-11	0.17334398764570125
1101	12-13	0.15051844676420956
1101	14-15	0.23930429267977615
1101	16-17	0.2585752250105102
1101	18-19	0.23088001359352717
1101	20-21	0.2004897804387724
1101	22-23	0.26107626771427306
1101	24-25	0.19660621257163058
1101	26-27	0.11431630866907483
1101	28-29	0.21357901927531486
1101	30-31	0.31801751990271754
1101	32-33	0.387657965332302
1101	34-35	0.32267444882555907
1101	36-37	0.33207611368963086
1101	38-39	0.3020540966516947
1101	40-41	0.31689662389488404
1101	42-43	0.34917700616504277
1101	44-45	0.27262152150592556
1101	46-47	0.403532127695712
1101	48-49	0.4425664550574737
1101	50-51	0.485627220466192
1101	52-53	0.36448951275024655
1101	54-55	0.348670758679944
1101	56-57	0.3599630904083426
1101	58-59	0.4060988838665054
1101	60-61	0.29229715242492915
1101	62-63	0.3356432011475263
1101	64-65	0.3541685811509012
1101	66-67	0.32191854757955696
1101	68-69	0.2841628985307736
1101	70-71	0.26124314890343214
1101	72-73	0.1524608665206415
1101	74-75	0.2093356661581396
1101	76-77	0.23080752935386073
1101	78-79	0.30327685797283976
1101	80-81	0.31081703976514774
1101	82-83	0.2747186535178088
1101	84-85	0.22747561830156826
1101	86-87	0.2583498910900275
1101	88-89	0.17888534575421033
1101	90-91	0.14886857967081824
1101	92-93	0.051599404613625666
1101	94-95	0.0774641442759112
1101	96-97	-0.07855677965147834
1101	98-99	-0.17010246863573641
1101	100	-0.13168513746197164
1102	1	0.31124673770884215
1102	2	0.19396906480555032
1102	3	0.300974995810833
1102	4	0.18223476926420545
1102	5	0.1940056134956336
1102	6	0.14440125949670346
1102	7	0.15380191248549835
1102	8	0.1740260969941403
1102	9	0.21337461525907742
1102	10-11	0.1967151407814356
1102	12-13	0.19288486815305106
1102	14-15	0.277182801498995
1102	16-17	0.24865455291509164
1102	18-19	0.22931148457114858
1102	20-21	0.24533196421861447
1102	22-23	0.2518656051106234
1102	24-25	0.2887079778296098
1102	26-27	0.21126904245926426
1102	28-29	0.33373230171482504
1102	30-31	0.3431399319800761
1102	32-33	0.37865912661646917
1102	34-35	0.3995110718748407
1102	36-37	0.46426537296002124
1102	38-39	0.4363954611694254
1102	40-41	0.412053432608225
1102	42-43	0.36570413019267534
1102	44-45	0.457664428923124
1102	46-47	0.3979567087408
1102	48-49	0.45614529600042886
1102	50-51	0.44954246263016984
1102	52-53	0.3766988868057126
1102	54-55	0.4465293824963439
1102	56-57	0.42743237470732254
1102	58-59	0.47214786637300676
1102	60-61	0.4399244738885315
1102	62-63	0.43091824166874204
1102	64-65	0.4172340001588424
1102	66-67	0.3516725038113435
1102	68-69	0.36037348866462793
1102	70-71	0.28633332076288553
1102	72-73	0.37147948418594723
1102	74-75	0.25411160194637006
1102	76-77	0.31733270907009725
1102	78-79	0.28597798372913275
1102	80-81	0.2999355950357341
1102	82-83	0.25739289475028926
1102	84-85	0.1928676179904798
1102	86-87	0.15138358247125083
1102	88-89	0.11814990113438739
1102	90-91	0.11664388090651912
1102	92-93	0.08225932157385074
1102	94-95	0.12122424806679177
1102	96-97	0.2285444641775456
1102	98-99	0.12136313561205725
1102	100	0.22823118644616613
1103	1	0.24928352869387993
1103	2	0.23163719236551827
1103	3	0.249860468086176
1103	4	0.14097374478865277
1103	5	0.18095066779436308
1103	6	0.19093357544298328
1103	7	0.20886658665176583
1103	8	0.19235828924389864
1103	9	0.205282804134292
1103	10-11	0.16926508118471162
1103	12-13	0.22294186412878503
1103	14-15	0.3223606405225823
1103	16-17	0.3184402528251269
1103	18-19	0.3469296464555285
1103	20-21	0.28206928004083665
1103	22-23	0.29089253805258863
1103	24-25	0.2964648342102265
1103	26-27	0.3036617063180955
1103	28-29	0.399994332488248
1103	30-31	0.4631653789095793
1103	32-33	0.4558861740650215
1103	34-35	0.4243692022561234
1103	36-37	0.4852696306568163
1103	38-39	0.46787147498006476
1103	40-41	0.44549836238333995
1103	42-43	0.4650535471372521
1103	44-45	0.5223598055588212
1103	46-47	0.5146178216235242
1103	48-49	0.5242601922414707
1103	50-51	0.46654930086067026
1103	52-53	0.37630715664425196
1103	54-55	0.48284231631870256
1103	56-57	0.47279109002794684
1103	58-59	0.4963637213999519
1103	60-61	0.5011750218383995
1103	62-63	0.5530296291926717
1103	64-65	0.6208047952811881
1103	66-67	0.5181113121708734
1103	68-69	0.4933494284962805
1103	70-71	0.5236393465112386
1103	72-73	0.45654766890731224
1103	74-75	0.468199974197546
1103	76-77	0.5037017198747016
1103	78-79	0.5554605168795277
1103	80-81	0.515359191296163
1103	82-83	0.5024006005106401
1103	84-85	0.47308680712576745
1103	86-87	0.44180274458813074
1103	88-89	0.49937159268893083
1103	90-91	0.4815909239071381
1103	92-93	0.44122064757116775
1103	94-95	0.4605223024656908
1103	96-97	0.48376981516413053
1103	98-99	0.5053694233654582
1103	100	0.6392039134766136
1104	1	0.2652110711513913
1104	2	0.22305347099491968
1104	3	0.28945857807622133
1104	4	0.09125721548641508
1104	5	0.18220928986064422
1104	6	0.20718036466666234
1104	7	0.1882742219392739
1104	8	0.22234205969299126
1104	9	0.2126759365562947
1104	10-11	0.2536343719329821
1104	12-13	0.27962607944648
1104	14-15	0.3686725282198253
1104	16-17	0.2813270228830973
1104	18-19	0.34843936950158394
1104	20-21	0.2209330642565348
1104	22-23	0.24729873583768835
1104	24-25	0.2974137581828131
1104	26-27	0.3861666962271144
1104	28-29	0.3981580767666486
1104	30-31	0.29461946089288205
1104	32-33	0.32120149169799816
1104	34-35	0.4360613779931555
1104	36-37	0.4027468015367006
1104	38-39	0.4698613169201451
1104	40-41	0.6112967883865608
1104	42-43	0.6507473880425891
1104	44-45	0.5975305136643385
1104	46-47	0.47550299464020895
1104	48-49	0.5434513995910137
1104	50-51	0.5503660736850335
1104	52-53	0.5635041013094266
1104	54-55	0.5593783813072122
1104	56-57	0.6041368260321107
1104	58-59	0.6567813343272277
1104	60-61	0.5741116128695651
1104	62-63	0.5374599882550584
1104	64-65	0.6736967229367679
1104	66-67	0.681009204507518
1104	68-69	0.7295932090900621
1104	70-71	0.6359292372218519
1104	72-73	0.6918781006710546
1104	74-75	0.6718072654849792
1104	76-77	0.6218420361988457
1104	78-79	0.7353506896825195
1104	80-81	0.7367069395633337
1104	82-83	0.6308554923565737
1104	84-85	0.6672054783651831
1104	86-87	0.7435558076182325
1104	88-89	0.71089276174839
1104	90-91	0.5821893900053929
1104	92-93	0.6062938063636558
1104	94-95	0.7096113009002707
1104	96-97	0.5991494666257537
1104	98-99	0.6610085500958895
1104	100	0.7981444866703704
1105	1	0.25118589544443637
1105	2	0.14420399172310283
1105	3	0.21876536220707266
1105	4	0.13202154545185607
1105	5	0.11347127568863868
1105	6	0.13374926511008312
1105	7	0.1834324998855621
1105	8	0.1637602707403758
1105	9	0.18124824433093778
1105	10-11	0.18513000483355313
1105	12-13	0.23042469243146968
1105	14-15	0.3046662712737458
1105	16-17	0.31343703446285787
1105	18-19	0.307199514940784
1105	20-21	0.2378725333917444
1105	22-23	0.27327961759175423
1105	24-25	0.28007924622897207
1105	26-27	0.3153913449747989
1105	28-29	0.36652999750718607
1105	30-31	0.39810790150744424
1105	32-33	0.47873672441378545
1105	34-35	0.5013117858702145
1105	36-37	0.558237537807976
1105	38-39	0.5269231409051613
1105	40-41	0.5468338542002371
1105	42-43	0.4985421422618046
1105	44-45	0.5535666619519404
1105	46-47	0.5771614002900165
1105	48-49	0.636192619805243
1105	50-51	0.6831336644120327
1105	52-53	0.6451753472131756
1105	54-55	0.6270288298028603
1105	56-57	0.625328093126079
1105	58-59	0.7091720395858587
1105	60-61	0.6955466816828917
1105	62-63	0.62999755180428
1105	64-65	0.6713596133175486
1105	66-67	0.6894939718608697
1105	68-69	0.6859544789515652
1105	70-71	0.6988067574495886
1105	72-73	0.7110650652314163
1105	74-75	0.7064161958316149
1105	76-77	0.7160749221614857
1105	78-79	0.8702515642449029
1105	80-81	0.8091425304653157
1105	82-83	0.7940554169821752
1105	84-85	0.7624570798454329
1105	86-87	0.7482119311408297
1105	88-89	0.7093676292606901
1105	90-91	0.8039434996307797
1105	92-93	0.7509950237336795
1105	94-95	0.8372230783062058
1105	96-97	0.8681558498472413
1105	98-99	0.7739498882573521
1105	100	0.8639600521278545
1106	1	0.23387934216867734
1106	2	0.18623362423399925
1106	3	0.20442217052397638
1106	4	0.14187809032937082
1106	5	0.11674772939372957
1106	6	0.12005616315550327
1106	7	0.11794350157404665
1106	8	0.12071964538123581
1106	9	0.09768543289636966
1106	10-11	0.11853488892317188
1106	12-13	0.14966158750067393
1106	14-15	0.23380661978578843
1106	16-17	0.20205175288622712
1106	18-19	0.19845433655931544
1106	20-21	0.1582401514164289
1106	22-23	0.2198805975332334
1106	24-25	0.20648464305295988
1106	26-27	0.1763606928886503
1106	28-29	0.27861025148750684
1106	30-31	0.26480939506732426
1106	32-33	0.3231825624250817
1106	34-35	0.2949150193382195
1106	36-37	0.3052370840085814
1106	38-39	0.2876190304845707
1106	40-41	0.34394300105739006
1106	42-43	0.3055302108739184
1106	44-45	0.33943167652361694
1106	46-47	0.4176098623815605
1106	48-49	0.3415733287636584
1106	50-51	0.41091279892230403
1106	52-53	0.4725865248225958
1106	54-55	0.36275024559802915
1106	56-57	0.4330071744475248
1106	58-59	0.44095271229925714
1106	60-61	0.4264078396964237
1106	62-63	0.4906229035724792
1106	64-65	0.5368391636141325
1106	66-67	0.4812876802564574
1106	68-69	0.4277704721386826
1106	70-71	0.43685354519818276
1106	72-73	0.556168359844456
1106	74-75	0.5673996513077029
1106	76-77	0.5756573825203404
1106	78-79	0.606273051204056
1106	80-81	0.5779473400021757
1106	82-83	0.5658665001482888
1106	84-85	0.5827663153997307
1106	86-87	0.5480158802268953
1106	88-89	0.5968851071674379
1106	90-91	0.5015106449968663
1106	92-93	0.5732374373276237
1106	94-95	0.5817661222098245
1106	96-97	0.5510506884685142
1106	98-99	0.7084411467255727
1106	100	0.8351439381240162
1107	1	0.2007400467951399
1107	2	0.1730124746934223
1107	3	0.2344169314990623
1107	4	0.11448896063439662
1107	5	0.08400464460810042
1107	6	0.1579302832679872
1107	7	0.12815646569791994
1107	8	0.1864969465221833
1107	9	0.1742162431391847
1107	10-11	0.19938554387591267
1107	12-13	0.23023943254494839
1107	14-15	0.2521713798056098
1107	16-17	0.26476631409177287
1107	18-19	0.21902571864692533
1107	20-21	0.2756495730041948
1107	22-23	0.2859683220948597
1107	24-25	0.331691927835017
1107	26-27	0.3437279858501441
1107	28-29	0.3761867546846531
1107	30-31	0.28939643004789417
1107	32-33	0.28249828755986783
1107	34-35	0.32266655223810403
1107	36-37	0.3811180163460506
1107	38-39	0.4324174698223331
1107	40-41	0.3234160611255703
1107	42-43	0.36041937042087824
1107	44-45	0.36686330799477673
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2316	80-81	-0.16003891981224427
2316	82-83	-0.12009585968685599
2316	84-85	-0.10682392544206465
2316	86-87	-0.12523723966442546
2316	88-89	-0.11962283071103386
2316	90-91	0.045507300611522083
2316	92-93	0.11809580462287528
2316	94-95	0.021645902781305182
2316	96-97	0.03248603994557442
2316	98-99	-0.03159362106701735
2316	100	-0.12891744660117865
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	4.0
11	27.0
12	71.0
13	211.0
14	612.0
15	1665.0
16	3770.0
17	7864.0
18	14365.0
19	21918.0
20	30832.0
21	41816.0
22	54419.0
23	61877.0
24	66706.0
25	73226.0
26	84144.0
27	101168.0
28	121653.0
29	146408.0
30	176979.0
31	213568.0
32	262567.0
33	329168.0
34	433581.0
35	593455.0
36	868207.0
37	1351487.0
38	1374953.0
39	228328.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.34445805146937	20.052385212108046	13.651378619019338	23.95177811740325
2	15.13101395208056	21.284760090515565	39.863617091298686	23.720608866105188
3	17.320102085061883	26.504411833105028	31.030753049873745	25.144733031959348
4	11.909777053268208	17.552319601743125	38.324572452249015	32.21333089273966
5	12.362368077030816	38.31356154844831	35.77425491401385	13.549815460507016
6	30.758823994525464	38.08443974129476	17.938235001546126	13.21850126263365
7	26.624881753195943	33.04392967231476	22.76202028931364	17.569168285175657
8	25.49650263615113	36.51609447533541	20.407830734191347	17.57957215432212
9	26.29842007921584	15.087168483023689	20.508828024745686	38.105583413014784
10-11	24.355023229261143	26.538543769509314	29.29270219359543	19.813730807634112
12-13	24.90952478475518	24.384295174030036	29.143469288088596	21.56271075312618
14-15	22.231569439849604	25.8559868328761	26.686741429404126	25.225702297870168
16-17	21.820369079638475	28.637501493802326	27.475857791369094	22.066271635190105
18-19	21.59835919533655	27.78718301401083	29.363966888333838	21.250490902318784
20-21	22.316826948720873	27.053666724407265	29.44504281444239	21.18446351242947
22-23	22.112163610816665	26.94609296055489	29.197797177380338	21.743946251248108
24-25	21.88334502986272	27.09148011296184	29.3127129334108	21.712461923764632
26-27	21.920787867807988	27.180509153869554	29.276598809241154	21.622104169081304
28-29	21.876254683401182	27.205362077998775	29.37422768516501	21.544155553435036
30-31	22.154132587465636	27.113788184796704	29.34632750331621	21.38575172442145
32-33	21.918316094481423	27.19053601358883	29.277053009300836	21.61409488262892
34-35	22.151308396470213	27.26752423940727	29.26969429099982	21.3114730731227
36-37	22.14820211714738	27.236907213586903	29.126005287917224	21.488885381348492
38-39	22.03228183825142	27.489390075237967	29.046835207155723	21.431492879354884
40-41	22.150048782903138	27.250463540734753	29.141844777716138	21.45764289864597
42-43	21.981780866849263	27.29837551664441	29.008549896603597	21.711293719902734
44-45	21.999116847815895	27.50663451948704	28.877598498251178	21.61665013444589
46-47	21.99708221430615	27.42512239590328	28.632666445899652	21.945128943890918
48-49	21.919230909180257	27.527482400197766	28.693722740328692	21.85956395029329
50-51	22.041122119079773	27.695966796085926	28.48681198205631	21.776099102777987
52-53	22.07305089057551	27.743046904721126	28.324518015829238	21.85938418887413
54-55	21.94854003310013	27.702877832822963	28.442615700978635	21.90596643309827
56-57	22.06172535260136	27.75958178089709	28.200441790252036	21.978251076249517
58-59	21.996068042106728	27.71182586674945	28.4054849957381	21.88662109540572
60-61	22.193758298070403	27.8036046251401	28.30250354242314	21.700133534366362
62-63	22.011012607702675	27.816708132133282	28.381428772932303	21.79085048723174
64-65	21.922630203382745	27.594701314648567	28.417371324039664	22.065297157929027
66-67	21.93623330105745	27.64874927581834	28.267707957737954	22.147309465386257
68-69	21.807306971036887	27.724812347671403	28.337161594642463	22.130719086649254
70-71	21.87447719210957	27.52198025037162	28.341450437699457	22.262092119819354
72-73	21.820667820776602	27.782094540734892	28.08812998553924	22.309107652949265
74-75	21.842263634239888	27.774999538627256	28.03147027266604	22.351266554466818
76-77	21.716886035062533	27.72251973475339	28.163554638244705	22.397039591939375
78-79	21.70969153103727	27.62222025660595	28.120999136254195	22.547089076102587
80-81	21.67855839083388	27.85990677176337	27.922024919580256	22.539509917822492
82-83	21.810835444922265	27.832682924926072	27.96838183810428	22.38809979204738
84-85	21.74924684286252	27.82028011022895	27.86117178585427	22.56930126105426
86-87	21.593435700114952	27.802223115963997	28.006271366014268	22.598069817906783
88-89	21.578876441994282	27.680595189930862	27.975679533615622	22.764848834459237
90-91	21.758468218868625	27.68833181747887	27.928139719036295	22.625060244616215
92-93	21.66687591210311	27.650872651026116	27.903963323690036	22.778288113180743
94-95	21.45376486818453	27.57640590397984	27.969023460169133	23.000805767666495
96-97	21.450607001405853	27.5006392963778	27.989093243627742	23.059660458588603
98-99	21.353927035322652	27.436222610184274	27.946158110242646	23.26369224425042
100	20.548778163642424	27.927471652153972	28.116192179202866	23.407558005000734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3108.0
1	2242.5
2	2321.5
3	3226.0
4	2449.5
5	1927.0
6	2861.0
7	5651.5
8	9007.0
9	10492.0
10	8885.0
11	6812.0
12	6771.5
13	7264.0
14	7719.0
15	8162.0
16	8657.0
17	9261.0
18	10089.5
19	10997.5
20	12440.0
21	14598.0
22	17378.5
23	21392.0
24	26921.0
25	33694.0
26	42089.0
27	51718.5
28	61473.0
29	72698.5
30	86158.0
31	98703.5
32	112001.5
33	127894.0
34	143417.0
35	156674.5
36	172637.5
37	188793.0
38	200245.5
39	210502.5
40	216249.5
41	219712.0
42	224526.5
43	229640.5
44	234990.5
45	241079.0
46	245107.5
47	246581.5
48	249470.0
49	250700.5
50	249419.5
51	242000.5
52	229757.5
53	219648.5
54	207527.0
55	191209.5
56	172522.0
57	153775.5
58	135746.0
59	115114.0
60	94051.0
61	75857.0
62	60121.5
63	46745.5
64	34840.5
65	25632.5
66	19096.5
67	14369.0
68	10811.0
69	8001.0
70	6038.5
71	4548.5
72	3404.5
73	2560.0
74	1994.0
75	1438.5
76	953.5
77	646.0
78	412.5
79	257.5
80	185.0
81	144.0
82	105.5
83	79.5
84	64.5
85	46.0
86	33.0
87	20.5
88	12.0
89	8.5
90	7.0
91	5.5
92	4.0
93	3.5
94	4.0
95	4.5
96	5.0
97	3.5
98	2.5
99	4.0
100	4.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0034058080279546323
2	0.0686112780257116
3	8.25195777698259E-4
4	6.00142383780552E-4
5	0.0017404129129636005
6	7.201708605366623E-4
7	0.0
8	9.752313736433968E-4
9	6.0014238378055195E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	6.976655211448917E-4
18-19	9.377224746571124E-4
20-21	0.003293281330995779
22-23	0.0
24-25	3.75088989862845E-5
26-27	1.1252669695885348E-4
28-29	8.552028968872866E-4
30-31	0.0014403417210733247
32-33	0.002970704799713732
34-35	0.003998448631937927
36-37	0.00118528120796659
38-39	0.001485352399856866
40-41	0.005288754757066114
42-43	0.006646576900369613
44-45	0.0048311461894334435
46-47	0.006631573340775098
48-49	0.008889609059749426
50-51	0.008544527189075607
52-53	0.003983445072343413
54-55	0.0023480570765414092
56-57	0.002363060636135923
58-59	0.0031807546340369254
60-61	0.007629310053810266
62-63	0.013578221433034986
64-65	0.003930932613762615
66-67	0.00999237068994619
68-69	0.012490463362432735
70-71	0.005281252977268857
72-73	0.007464270898270614
74-75	0.0028806834421466494
76-77	0.0028956870017411633
78-79	0.007629310053810266
80-81	0.0054913028115920505
82-83	0.01600879808734622
84-85	0.01705904725896219
86-87	0.021980214805962715
88-89	0.020959972753535776
90-91	0.024403289680476694
92-93	0.03384052866542587
94-95	0.03742637940851467
96-97	0.022640371428121323
98-99	0.0180492821922001
100	0.018949495767870927
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	6665085.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.28716719525894
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.63360658576453	46.48715931792694
2	16.08917916673102	20.042987855853042
3	4.839148079890816	9.042504766043265
4	1.8058235319571327	4.499185290405878
5	0.8686605621486012	2.7053202835238777
6	0.48555585296313	1.814633915769063
7	0.2952347896616267	1.2872537093857592
8	0.18688319773909243	0.9312339986847589
9	0.13364787221787872	0.7492092625913053
>10	0.6084345467928952	6.531531621675358
>50	0.033140427767292306	1.4340444088557556
>100	0.018982440048197265	2.189673560219938
>500	0.001220981162416618	0.5393446460733763
>1k	3.8557212429579847E-4	0.512985933133243
>5k	0.0	0.0
>10k+	9.639303107055737E-5	1.2329314298586294
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	24011	0.3602504694238708	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	22353	0.3353745676161669	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15241	0.22866925177998482	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.5003559594513799E-5
2	0.0	0.0	0.0	0.0	1.5003559594513799E-5
3	0.0	0.0	0.0	0.0	1.5003559594513799E-5
4	0.0	0.0	0.0	0.0	1.5003559594513799E-5
5	0.0	0.0	0.0	0.0	1.5003559594513799E-5
6	0.0	0.0	0.0	0.0	3.0007119189027597E-5
7	0.0	0.0	0.0	0.0	3.0007119189027597E-5
8	0.0	0.0	0.0	0.0	4.5010678783541396E-5
9	0.0	0.0	0.0	0.0	6.0014238378055195E-5
10-11	0.0	0.0	0.0	0.0	6.0014238378055195E-5
12-13	0.0	0.0	0.0	1.5003559594513799E-5	6.0014238378055195E-5
14-15	0.0	0.0	0.0	3.0007119189027597E-5	9.002135756708279E-5
16-17	0.0	0.0	0.0	5.2512458580798296E-5	9.002135756708279E-5
18-19	0.0	0.0	0.0	6.0014238378055195E-5	9.002135756708279E-5
20-21	0.0	0.0	0.0	1.1252669695885349E-4	9.002135756708279E-5
22-23	0.0	0.0	0.0	2.1004983432319318E-4	9.002135756708279E-5
24-25	0.0	0.0	0.0	3.6008543026833117E-4	9.002135756708279E-5
26-27	0.0	0.0	0.0	5.851388241860382E-4	1.2002847675611039E-4
28-29	0.0	0.0	0.0	0.0019954734260703355	1.2002847675611039E-4
30-31	0.0	0.0	0.0	0.006031430956994547	1.575373757423949E-4
32-33	0.0	0.0	0.0	0.014898534677352202	1.6503915553965179E-4
34-35	0.0	0.0	0.0	0.028379232973022847	1.8004271513416558E-4
36-37	0.0	0.0	0.0	0.05085456524560452	1.8004271513416558E-4
38-39	0.0	0.0	0.0	0.08471009747062491	1.9504627472867938E-4
40-41	0.0	0.0	0.0	0.12405693250723734	1.9504627472867938E-4
42-43	0.0	0.0	0.0	0.1614307994571712	1.9504627472867938E-4
44-45	0.0	0.0	0.0	0.21005733610299043	1.9504627472867938E-4
46-47	0.0	0.0	0.0	0.2692988911619282	2.1004983432319318E-4
48-49	0.0	0.0	0.0	0.33387421165671555	2.4005695351222078E-4
50-51	0.0	0.0	0.0	0.40184033661986307	2.5506051310673455E-4
52-53	0.0	0.0	0.0	0.47174192077070287	2.700640727012484E-4
54-55	0.0	0.0	0.0	0.5526711212235103	2.700640727012484E-4
56-57	0.0	0.0	0.0	0.6499992123131213	2.700640727012484E-4
58-59	0.0	0.0	0.0	0.7596827347288144	2.8506763229576215E-4
60-61	0.0	0.0	0.0	0.8772131188124382	3.00071191890276E-4
62-63	0.0	0.0	0.0	0.9996196597642791	3.3007831107930357E-4
64-65	0.0	0.0	0.0	1.1270748985196737	3.3007831107930357E-4
66-67	0.0	0.0	0.0	1.2737121882166544	3.7508898986284494E-4
68-69	7.501779797256899E-6	0.0	0.0	1.4462981342323467	3.8259076966010185E-4
70-71	1.5003559594513799E-5	0.0	0.0	1.6391988999390106	3.9009254945735877E-4
72-73	1.5003559594513799E-5	0.0	0.0	1.8304117651912917	4.2009966864638636E-4
74-75	1.5003559594513799E-5	0.0	0.0	2.0252404883058506	4.2009966864638636E-4
76-77	1.5003559594513799E-5	0.0	0.0	2.242079433345561	4.351032282409002E-4
78-79	1.5003559594513799E-5	0.0	0.0	2.4952044872646035	4.5010678783541396E-4
80-81	1.5003559594513799E-5	0.0	0.0	2.767144004915166	4.5010678783541396E-4
82-83	1.5003559594513799E-5	0.0	0.0	3.0436521064622584	5.026192464162122E-4
84-85	1.5003559594513799E-5	0.0	0.0	3.3297474825902444	5.101210262134691E-4
86-87	1.5003559594513799E-5	0.0	0.0	3.6303212937269365	5.101210262134691E-4
88	1.5003559594513799E-5	0.0	0.0	3.87477429020035	5.101210262134691E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	11730	0.0	49.90764	1
GTATCAA	18880	0.0	36.28718	1
TACCTGG	5790	0.0	29.235893	2
TCAACGC	23580	0.0	28.477629	4
ATCAACG	23715	0.0	28.256071	3
CAACGCA	23860	0.0	28.123745	5
AACGCAG	24675	0.0	27.289646	6
TATCAAC	24835	0.0	27.264074	2
GTACCTG	6530	0.0	26.787086	1
GTACATG	21140	0.0	25.935179	1
TACATGG	21430	0.0	24.991594	2
ACATGGG	21255	0.0	24.341177	3
ACGCAGA	28210	0.0	23.753197	7
CGCAGAG	28645	0.0	23.228441	8
CATGGGG	12185	0.0	22.94599	4
ACCTGGG	7250	0.0	22.750162	3
GCAGAGT	32185	0.0	20.264614	9
GAGTACT	20670	0.0	19.91524	12-13
ATGGGGG	7775	0.0	19.884327	5
AGAGTAC	29630	0.0	19.340405	10-11
>>END_MODULE