FastQCFastQC Report
Mon 6 Jun 2016
ERR1211527_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211527_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8791062
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT280500.31907407773941304No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245530.2792950385289058No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT157580.179250243030933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA130800.051.6199531
GTATCAA226800.037.8875121
TCAACGC271650.030.789214
ATCAACG272750.030.75313
CAACGCA273800.030.6145175
AACGCAG285200.029.4679726
TATCAAC289700.029.166512
GTACATG264550.027.5155331
TACATGG265500.026.848472
ACGCAGA324000.025.7369827
ACATGGG269400.025.379373
CGCAGAG331100.025.0156758
TACCTGG72050.023.6895892
GTACCTG80200.022.6028161
CATGGGG163600.022.4601024
GCAGAGT377550.021.392799
ATGGGGG103850.019.997785
GAGTACT249550.019.9214112-13
CAGAGTA365100.019.54826510-11
AGAGTAC344300.019.35773810-11
Produced by FastQC (version 0.11.5)