FastQCFastQC Report
Mon 6 Jun 2016
ERR1211527_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211527_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8791062
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT308860.3513341164014086No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279540.31798205950543856No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198160.22541076379622849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145350.053.284731
GTATCAA247700.037.2475051
TCAACGC307500.029.5093694
ATCAACG307000.029.4502813
CAACGCA310050.029.2360235
AACGCAG322450.028.2135876
TATCAAC323400.028.1933842
GTACATG277800.027.4224971
TACATGG279100.026.636762
ACATGGG277700.025.8226853
TACCTGG70900.025.0037142
ACGCAGA368000.024.670037
CGCAGAG373500.024.1935238
GTACCTG77450.023.375641
CATGGGG164750.023.3034064
GCAGAGT420950.021.0197589
GAGTACT278450.020.4800112-13
AGAGTAC387750.019.88769310-11
ATGGGGG100600.019.8054945
CAGAGTA412750.019.36052910-11
Produced by FastQC (version 0.11.5)