FastQCFastQC Report
Mon 6 Jun 2016
ERR1211526_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211526_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3566064
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97630.2737752323009346No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84850.23793740101131106No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62360.17487066973559645No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG41200.045.0773622
GGTATCA52650.044.4372631
GTACCTG45300.042.6064071
ACCTGGG48600.037.4156233
GTATCAA86250.033.9484981
TCAACGC100800.028.3868224
GTACATG110600.028.2193951
CAACGCA101800.028.152945
ATCAACG102450.027.8390883
TACATGG112100.027.1367152
AACGCAG107350.026.9113566
ACATGGG110150.026.7034953
CATGGGG66450.026.585994
TATCAAC111650.025.7721882
CCTGGGG43950.025.4435714
ATGGGGG45200.024.1152215
ACGCAGA123100.023.3168587
CGCAGAG124900.022.8686338
TATAACG4000.021.157832
GTATAAC11700.019.7151241
Produced by FastQC (version 0.11.5)