##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211526_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3566064 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.121189916950453 31.0 31.0 34.0 28.0 34.0 2 31.153609133206807 31.0 31.0 34.0 27.0 34.0 3 31.25911957833623 33.0 31.0 34.0 27.0 34.0 4 34.762965835722525 37.0 35.0 37.0 32.0 37.0 5 34.85210641199934 37.0 35.0 37.0 32.0 37.0 6 34.97715184023618 37.0 35.0 37.0 32.0 37.0 7 34.89029781854728 37.0 35.0 37.0 32.0 37.0 8 34.84786868659676 37.0 35.0 37.0 32.0 37.0 9 36.40246557549163 39.0 37.0 39.0 32.0 39.0 10-11 36.39132780567034 39.0 37.0 39.0 32.0 39.0 12-13 36.334690011171986 39.0 37.0 39.0 31.5 39.0 14-15 37.50579686735853 40.0 37.0 41.0 31.0 41.0 16-17 37.49324717671921 40.0 37.0 41.0 31.0 41.0 18-19 37.494026747697184 40.0 37.0 41.0 31.0 41.0 20-21 37.430799615486436 40.0 37.0 41.0 31.0 41.0 22-23 37.25887210661391 40.0 37.0 41.0 31.0 41.0 24-25 37.19390131528767 40.0 37.0 41.0 30.5 41.0 26-27 37.05971121101584 40.0 36.5 41.0 30.0 41.0 28-29 36.904520221734664 40.0 36.0 41.0 30.0 41.0 30-31 36.73534644919441 39.5 36.0 41.0 30.0 41.0 32-33 36.58051986728225 39.0 35.5 41.0 29.5 41.0 34-35 36.40476951619489 39.0 35.0 41.0 29.0 41.0 36-37 36.236928445479386 39.0 35.0 41.0 28.0 41.0 38-39 36.106402885646474 39.0 35.0 40.0 27.5 41.0 40-41 36.06799948065991 39.0 35.0 40.0 27.0 41.0 42-43 36.02255413811979 39.0 35.0 40.0 27.0 41.0 44-45 35.84766594205824 39.0 35.0 40.0 27.0 41.0 46-47 35.82252589970342 39.0 35.0 40.0 26.5 41.0 48-49 35.759080599787325 39.0 35.0 41.0 26.0 41.0 50-51 34.82079485954262 38.0 34.0 40.0 25.0 40.5 52-53 35.03170961037155 38.0 34.0 39.5 25.0 40.5 54-55 35.623348038621856 39.0 35.0 40.5 26.0 41.0 56-57 35.600270354093475 39.0 34.5 41.0 26.0 41.0 58-59 35.44954184781877 38.5 34.0 40.5 26.0 41.0 60-61 35.27188252930962 38.0 34.0 40.0 26.0 41.0 62-63 35.02460892457343 38.0 34.0 40.0 25.5 41.0 64-65 34.75603087886252 37.5 34.0 40.0 25.0 41.0 66-67 34.47002367315898 37.0 33.0 40.0 24.5 41.0 68-69 34.199200575199995 36.5 33.0 39.5 24.0 41.0 70-71 33.85352534334774 36.0 33.0 39.0 24.0 41.0 72-73 33.519024336074736 35.5 32.5 39.0 23.5 40.5 74-75 33.15768155030308 35.0 32.0 38.0 23.0 40.0 76-77 32.80908166538795 35.0 32.0 37.0 23.0 39.0 78-79 32.49181660789038 35.0 32.0 37.0 22.0 39.0 80-81 32.13466541823142 35.0 31.0 36.0 21.5 39.0 82-83 31.827158177755642 35.0 31.0 36.0 21.0 37.0 84-85 31.568291679566045 35.0 31.0 35.5 20.0 37.0 86-87 31.267046104612817 34.5 31.0 35.0 20.0 36.5 88-89 31.04440596130636 34.0 30.5 35.0 20.0 36.0 90-91 30.815722740814522 34.0 30.0 35.0 19.0 36.0 92-93 30.662973659474424 34.0 30.0 35.0 19.0 36.0 94-95 30.513290423279 34.0 30.0 35.0 18.0 35.0 96-97 30.425337150426913 34.0 30.0 35.0 18.0 35.0 98-99 30.24689139062002 34.0 29.0 35.0 18.0 35.0 100 28.905477299341797 33.0 26.0 35.0 13.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3765617796516203 1101 2 0.3666169859235566 1101 3 0.3213817340834346 1101 4 0.3414613766267607 1101 5 0.25872312820080623 1101 6 0.35051983178814794 1101 7 0.2663001304869823 1101 8 0.37268796514072733 1101 9 0.35143921513306964 1101 10-11 0.4052169762072495 1101 12-13 0.21268998564721642 1101 14-15 0.3213541896525598 1101 16-17 0.3507622613976622 1101 18-19 0.36749910251784 1101 20-21 0.458097038207363 1101 22-23 0.5300986676656834 1101 24-25 0.446223907702489 1101 26-27 0.39851180642802575 1101 28-29 0.51563406971691 1101 30-31 0.4174437276211336 1101 32-33 0.31946478579466486 1101 34-35 0.46625571452651116 1101 36-37 0.4311221718156162 1101 38-39 0.4564212512163621 1101 40-41 0.22908955288531274 1101 42-43 0.2339407846995627 1101 44-45 0.2602303175947256 1101 46-47 0.28670250123139596 1101 48-49 0.3859554462867507 1101 50-51 0.38387660891629594 1101 52-53 0.5332736138482161 1101 54-55 0.5115024661816818 1101 56-57 0.5163119664614868 1101 58-59 0.5509126775928834 1101 60-61 0.5508044543540151 1101 62-63 0.5531491978408596 1101 64-65 0.5376071772873487 1101 66-67 0.3640739220276643 1101 68-69 0.38731410711375247 1101 70-71 0.2834692233164944 1101 72-73 0.43905934161752924 1101 74-75 0.2774845280631766 1101 76-77 0.303843411166703 1101 78-79 0.17633652997767513 1101 80-81 0.24041856961254382 1101 82-83 0.1486794563438245 1101 84-85 0.19713178451295477 1101 86-87 0.16034318850353202 1101 88-89 0.08374338809620596 1101 90-91 0.08101334938681148 1101 92-93 0.1149094834346549 1101 94-95 -0.0010799210173821905 1101 96-97 -0.04197520496846252 1101 98-99 -0.05128213827489603 1101 100 0.02902973617826987 1102 1 0.4147212304007972 1102 2 0.3942147338354651 1102 3 0.3396461481400763 1102 4 0.2670218248679177 1102 5 0.3121371366960446 1102 6 0.33700268414077783 1102 7 0.46301531994664913 1102 8 0.35853760854967476 1102 9 0.38347756780336084 1102 10-11 0.35911649969560244 1102 12-13 0.40122176372502594 1102 14-15 0.3225724983787117 1102 16-17 0.36000720592336677 1102 18-19 0.3872074177303162 1102 20-21 0.450432141023569 1102 22-23 0.41409088251516835 1102 24-25 0.40587883701255123 1102 26-27 0.4239296495639806 1102 28-29 0.36824725053102725 1102 30-31 0.5010069528060654 1102 32-33 0.3932049736200369 1102 34-35 0.43035124859495966 1102 36-37 0.5214224802387974 1102 38-39 0.4586047573290486 1102 40-41 0.3978757888895217 1102 42-43 0.4207833792747735 1102 44-45 0.22219080701638205 1102 46-47 0.2930975749159117 1102 48-49 0.30873718923194104 1102 50-51 0.3402273900736361 1102 52-53 0.44287121535796103 1102 54-55 0.5354165946562404 1102 56-57 0.5077391018290811 1102 58-59 0.48866572532195107 1102 60-61 0.40295310187593003 1102 62-63 0.4917567409971966 1102 64-65 0.4979975230430753 1102 66-67 0.3455261375739269 1102 68-69 0.2852735192659921 1102 70-71 0.4270662619216452 1102 72-73 0.27318905315437547 1102 74-75 0.1670724764247069 1102 76-77 0.199361469325936 1102 78-79 0.11951925827991516 1102 80-81 0.2503970516256331 1102 82-83 0.21886528140419514 1102 84-85 0.11889121756860632 1102 86-87 0.12013109325756943 1102 88-89 0.2139382478143581 1102 90-91 0.07825413828310346 1102 92-93 0.16488217982200482 1102 94-95 0.12794383880163807 1102 96-97 -0.1538786406045496 1102 98-99 -0.1794120228324836 1102 100 -0.14205035559318446 1103 1 0.3537124565471359 1103 2 0.43372234530781384 1103 3 0.3237860577813798 1103 4 0.3751501871660068 1103 5 0.3358778191103369 1103 6 0.3413905814009439 1103 7 0.4162126351932329 1103 8 0.4625427293158282 1103 9 0.4411042759061061 1103 10-11 0.4513811616345862 1103 12-13 0.3693766692303484 1103 14-15 0.4541797986534988 1103 16-17 0.41061545217353057 1103 18-19 0.36894469742810543 1103 20-21 0.4656814037877339 1103 22-23 0.4935474959388557 1103 24-25 0.4406937196855978 1103 26-27 0.5021913140381216 1103 28-29 0.41662374018992665 1103 30-31 0.3543102390321238 1103 32-33 0.2661367636329075 1103 34-35 0.46095756951157796 1103 36-37 0.5563623155063198 1103 38-39 0.4151847982190944 1103 40-41 0.3090278564687239 1103 42-43 0.4040295226880559 1103 44-45 0.2704468080597877 1103 46-47 0.27477919073656665 1103 48-49 0.3799948318551074 1103 50-51 0.3807031027284751 1103 52-53 0.3606137074305167 1103 54-55 0.4233622986975405 1103 56-57 0.48929560978641007 1103 58-59 0.3739106056232657 1103 60-61 0.37427129934449965 1103 62-63 0.4399348359029389 1103 64-65 0.396325588234248 1103 66-67 0.4498601177313475 1103 68-69 0.4024578586960885 1103 70-71 0.37358352374022985 1103 72-73 0.31352533439591923 1103 74-75 0.32422681791599217 1103 76-77 0.2350039348863433 1103 78-79 0.2893188489136307 1103 80-81 0.3970854973341389 1103 82-83 0.25751045213586465 1103 84-85 0.2048916748353271 1103 86-87 0.3244788923412507 1103 88-89 0.3399832749999874 1103 90-91 0.1928670705466864 1103 92-93 0.18632845467035608 1103 94-95 0.1959298506323499 1103 96-97 0.16684963233632288 1103 98-99 0.2052612740721962 1103 100 0.23698936101013857 1104 1 0.462707128306608 1104 2 0.4763369537875022 1104 3 0.44556487956957014 1104 4 0.4899565760323128 1104 5 0.3804427649811899 1104 6 0.3589796577754356 1104 7 0.33411973809042905 1104 8 0.40038010400855484 1104 9 0.40187849877641213 1104 10-11 0.47948802375455557 1104 12-13 0.4406692746351055 1104 14-15 0.45058569514564084 1104 16-17 0.5796226813032206 1104 18-19 0.388656366195022 1104 20-21 0.4640897210730017 1104 22-23 0.5302817953314616 1104 24-25 0.5222018410923184 1104 26-27 0.6201374329837464 1104 28-29 0.6685342593552619 1104 30-31 0.5646162731965134 1104 32-33 0.4635028625930815 1104 34-35 0.4872573235957631 1104 36-37 0.5723542450591808 1104 38-39 0.5267904992744761 1104 40-41 0.4172234897870055 1104 42-43 0.2958915531362365 1104 44-45 0.3234264010543555 1104 46-47 0.49229707096070996 1104 48-49 0.46413622651064657 1104 50-51 0.5154530942610478 1104 52-53 0.677976554278267 1104 54-55 0.6489948427012351 1104 56-57 0.7334518249599995 1104 58-59 0.6287195428560182 1104 60-61 0.6671528933880211 1104 62-63 0.6126506435177106 1104 64-65 0.5310148157978674 1104 66-67 0.5173077744936734 1104 68-69 0.4389986667003143 1104 70-71 0.4397889293618107 1104 72-73 0.5840020362578926 1104 74-75 0.5747253498538569 1104 76-77 0.6403213479564656 1104 78-79 0.5329610892656689 1104 80-81 0.45178767909388995 1104 82-83 0.5323246473120626 1104 84-85 0.39782212031969877 1104 86-87 0.4656353748297981 1104 88-89 0.5593812730287233 1104 90-91 0.4655551468501109 1104 92-93 0.5186947315046417 1104 94-95 0.4931765685448468 1104 96-97 0.5281306065563562 1104 98-99 0.48446128696903656 1104 100 0.6320263863410425 1105 1 0.38688312010857473 1105 2 0.40938469874097905 1105 3 0.3315247655167468 1105 4 0.3533092832224156 1105 5 0.33578967565936324 1105 6 0.33291686614087723 1105 7 0.3988748335060208 1105 8 0.37304908093593525 1105 9 0.5325562056731599 1105 10-11 0.45948722194285097 1105 12-13 0.43983666162854007 1105 14-15 0.4782525990964288 1105 16-17 0.4046996356703545 1105 18-19 0.40118418514985876 1105 20-21 0.500949102856616 1105 22-23 0.5153294737932299 1105 24-25 0.5821959350646466 1105 26-27 0.5688957982265705 1105 28-29 0.5305057969954348 1105 30-31 0.5240768690403925 1105 32-33 0.5608124909826913 1105 34-35 0.5586972796330159 1105 36-37 0.5522238822100505 1105 38-39 0.5911921130672013 1105 40-41 0.7022543497593858 1105 42-43 0.6172288381626956 1105 44-45 0.6290745778127658 1105 46-47 0.6372103579584518 1105 48-49 0.6413219456575163 1105 50-51 0.6732814165971703 1105 52-53 0.7020392168406957 1105 54-55 0.6111903987087572 1105 56-57 0.6450755523283789 1105 58-59 0.7299702713312897 1105 60-61 0.7091419144346034 1105 62-63 0.6974436978727212 1105 64-65 0.6662144473556069 1105 66-67 0.5447546691356138 1105 68-69 0.5509936720000965 1105 70-71 0.6029189871313676 1105 72-73 0.5705907577006855 1105 74-75 0.5113269785094374 1105 76-77 0.5167577642883927 1105 78-79 0.47007411733689963 1105 80-81 0.5544166436616322 1105 82-83 0.6130245507035248 1105 84-85 0.4636107705772581 1105 86-87 0.4935560228501039 1105 88-89 0.6490101319920001 1105 90-91 0.3791335347001201 1105 92-93 0.32981820659567873 1105 94-95 0.44162643184573014 1105 96-97 0.4648593517014845 1105 98-99 0.4937080337647224 1105 100 0.5392733951922608 1106 1 0.42991340373640696 1106 2 0.4355105690884784 1106 3 0.4168325808258757 1106 4 0.40898891671182724 1106 5 0.24417157771090814 1106 6 0.2593535819014079 1106 7 0.3281338425188238 1106 8 0.36499515463457755 1106 9 0.4014420092291928 1106 10-11 0.4170228712977675 1106 12-13 0.41650507221969946 1106 14-15 0.5121163971898426 1106 16-17 0.49832114059013577 1106 18-19 0.4635313109320194 1106 20-21 0.4409319135461729 1106 22-23 0.5191804999565903 1106 24-25 0.5238896030742239 1106 26-27 0.5285016207439241 1106 28-29 0.4781364607482601 1106 30-31 0.6022914610547332 1106 32-33 0.5868946930117076 1106 34-35 0.6106967690928045 1106 36-37 0.603226091181071 1106 38-39 0.5672618043335973 1106 40-41 0.5716701406726727 1106 42-43 0.5366712513048597 1106 44-45 0.5016743648349333 1106 46-47 0.5406555636264017 1106 48-49 0.6142356531286524 1106 50-51 0.5671921855134201 1106 52-53 0.6020239539911216 1106 54-55 0.6210722162319158 1106 56-57 0.6364184134990722 1106 58-59 0.5452057544938498 1106 60-61 0.612143837525565 1106 62-63 0.7243747642727172 1106 64-65 0.890858950735911 1106 66-67 0.7080426576240271 1106 68-69 0.6683260207602828 1106 70-71 0.6203299388648134 1106 72-73 0.5605778650157944 1106 74-75 0.5990300221491083 1106 76-77 0.6252205299560529 1106 78-79 0.5311297982341046 1106 80-81 0.5049379113964463 1106 82-83 0.5329644456861296 1106 84-85 0.5392142498891452 1106 86-87 0.6148097444860561 1106 88-89 0.5504168977450306 1106 90-91 0.7086090318590159 1106 92-93 0.6829734462041621 1106 94-95 0.7497861028758592 1106 96-97 0.6855555931931825 1106 98-99 0.7102479218951956 1106 100 0.5799749358554678 1107 1 0.41498496077553426 1107 2 0.3973948203046831 1107 3 0.4262780603296328 1107 4 0.36501057543139126 1107 5 0.37785810951545784 1107 6 0.4297235629558216 1107 7 0.43068706852418615 1107 8 0.4184097329966576 1107 9 0.43969832559702837 1107 10-11 0.41675491002986575 1107 12-13 0.5003759834480306 1107 14-15 0.581753357608271 1107 16-17 0.5885045571466492 1107 18-19 0.5273076101061918 1107 20-21 0.5727043673209593 1107 22-23 0.6814434973540955 1107 24-25 0.5925827328645923 1107 26-27 0.6294116958392948 1107 28-29 0.6659394432088988 1107 30-31 0.6458454096243784 1107 32-33 0.7150188730916156 1107 34-35 0.7523204033180235 1107 36-37 0.7831779119520164 1107 38-39 0.8150225171974697 1107 40-41 0.6968772330039172 1107 42-43 0.796963929844317 1107 44-45 0.828965154333666 1107 46-47 0.7723300477268182 1107 48-49 0.7162449556954797 1107 50-51 0.7033196823813341 1107 52-53 0.7521064046731425 1107 54-55 0.772964457315112 1107 56-57 0.7663215529153504 1107 58-59 0.6334417099437246 1107 60-61 0.7643599772224334 1107 62-63 0.779309859018575 1107 64-65 0.7862904628706602 1107 66-67 0.8690743436818451 1107 68-69 0.7134921512285146 1107 70-71 0.6330816534720611 1107 72-73 0.6989794115626964 1107 74-75 0.6379414469139988 1107 76-77 0.6094702701336345 1107 78-79 0.5914705373685365 1107 80-81 0.7034903135847301 1107 82-83 0.7622859746531212 1107 84-85 0.7061548095274581 1107 86-87 0.6720084049694073 1107 88-89 0.7073201179980195 1107 90-91 0.7754938886607867 1107 92-93 0.8034699380842092 1107 94-95 0.723433405426686 1107 96-97 0.8329358769272623 1107 98-99 0.8589980153540182 1107 100 0.9948312603345606 1108 1 0.3530588853690695 1108 2 0.37418852615421727 1108 3 0.24108281754158511 1108 4 0.28190528697281536 1108 5 0.2503789259552889 1108 6 0.3039264551804095 1108 7 0.16464628011009808 1108 8 0.255971829755957 1108 9 0.1739483993268749 1108 10-11 0.22485836573515172 1108 12-13 0.1927592566502625 1108 14-15 0.37159475589550794 1108 16-17 0.3659475892984574 1108 18-19 0.30729117408662887 1108 20-21 0.34836053496947983 1108 22-23 0.401946942090845 1108 24-25 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80-81 -0.19889040169370986 2316 82-83 -0.08847133272984209 2316 84-85 -0.2052453146520712 2316 86-87 0.02131169088378826 2316 88-89 -0.0915071418571678 2316 90-91 -0.03193308920565485 2316 92-93 -0.08307101721660004 2316 94-95 -0.08731817729092128 2316 96-97 -0.170423894891492 2316 98-99 -0.10303723650404706 2316 100 -0.29960999164757496 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 13.0 1 29.0 2 54.0 3 65.0 4 72.0 5 72.0 6 169.0 7 1146.0 8 2217.0 9 2540.0 10 3819.0 11 6581.0 12 10122.0 13 12359.0 14 13313.0 15 14381.0 16 15632.0 17 17632.0 18 20521.0 19 23174.0 20 26246.0 21 30309.0 22 34026.0 23 36129.0 24 37230.0 25 38403.0 26 42016.0 27 46841.0 28 53598.0 29 62627.0 30 75096.0 31 91710.0 32 114480.0 33 148912.0 34 202611.0 35 284640.0 36 432683.0 37 700919.0 38 768890.0 39 194469.0 40 318.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.648529235822735 19.11414886990243 12.48406899158753 23.753252902687304 2 15.295072658380843 20.44489030719151 40.37709771233675 23.882939322090895 3 17.96946113075444 26.710220156439124 30.48742414020895 24.832894572597485 4 11.99034242211986 16.444222775086544 38.76204146632036 32.803393336473235 5 12.24677217890161 37.941150977792276 35.70380090327659 14.108275940029527 6 30.276448317336342 37.43582064188192 18.9242068736727 13.363524167109041 7 26.60839458921635 32.63590617389753 23.330471048673047 17.425228188213072 8 25.500941855661775 35.88059227675553 21.03149530558498 17.586970561997717 9 26.647314485194766 14.975851371656674 20.503786771902448 37.87304737124611 10-11 24.242707227802196 25.982630077065206 30.018888764094886 19.755773931037712 12-13 24.653959733802235 23.974877796380586 29.597001489159425 21.774160980657754 14-15 22.050936852718984 25.539624641869974 27.01207019138132 25.397368314029723 16-17 21.820697434211873 28.197096973492364 27.718653166661383 22.26355242563438 18-19 21.503145254716056 27.604351820337993 29.3537520714423 21.538750853503647 20-21 22.27020116421939 27.019793861335124 29.241002277137618 21.469002697307868 22-23 21.957951831531272 26.88348060375965 29.082283195180516 22.076284369528558 24-25 21.684041027556923 26.964029224939985 29.226973467391765 22.124956280111327 26-27 21.63100985639586 27.125533379794458 29.054745134552633 22.188711629257046 28-29 21.65082454975313 27.327592070690322 29.13892981387061 21.88265356568594 30-31 22.185180365313453 27.132205819907863 29.10130624548799 21.581307569290693 32-33 21.87711280686493 27.205288291160056 29.070245074026513 21.847353827948503 34-35 21.986896544180198 27.41101146909996 28.95998927445202 21.64210271226782 36-37 21.98410753951553 27.36673505315368 28.891007160596704 21.758150246734086 38-39 21.852647480698757 27.571063867025458 28.819355041379247 21.756933610896546 40-41 21.99227684213892 27.20058387903696 29.037253143090197 21.769886135733923 42-43 21.75939419356015 27.326580332610064 28.80973453189278 22.104290941937005 44-45 21.664369403796815 27.59926048099593 28.81504015920333 21.92132995600392 46-47 21.65885407719002 27.364290587658658 28.614328277413424 22.3625270577379 48-49 21.650913438555538 27.46185703221551 28.686669758275368 22.200559770953586 50-51 21.71502316099927 27.698235731056158 28.56604576332436 22.02069534462022 52-53 21.817571884804803 27.685558078075452 28.408248808347764 22.088621228771984 54-55 21.69894967441169 27.60684527858382 28.66789992384473 22.026305123159755 56-57 21.83732278579358 27.599277259419775 28.348425021274398 22.21497493351224 58-59 21.769241093649132 27.695663290099986 28.52576378768624 22.00933182856464 60-61 22.044127693431196 27.791914106968825 28.430019420291604 21.733938779308378 62-63 21.848919029587826 27.805355887608872 28.50273986303981 21.842985219763484 64-65 21.80325213172627 27.474531139438284 28.483257209018227 22.238959519817218 66-67 21.733445061128894 27.596629934400823 28.389135289972195 22.280789714498084 68-69 21.642291668273746 27.646837304512044 28.521896824856956 22.18897420235725 70-71 21.674573152846904 27.476487794778393 28.56719433151301 22.281744720861692 72-73 21.58145745898512 27.80186053818704 28.23310590928474 22.383576093543102 74-75 21.6532031257671 27.81148611401391 28.17486846221068 22.360442298008312 76-77 21.581808915582425 27.584124046593207 28.39816438776509 22.43590265005928 78-79 21.603895073983434 27.62051047749827 28.377769111212924 22.39782533730537 80-81 21.68595757141599 27.745202424073796 28.151767147102102 22.417072857408115 82-83 21.80961560494999 27.75304690395684 28.230807839217203 22.206529651875965 84-85 21.732294065345986 27.84544488167996 28.111170634976286 22.31109041799777 86-87 21.610719882376138 27.841690781297203 28.230624220429178 22.31696511589748 88-89 21.602294280775933 27.62605747622103 28.344787207561588 22.42686103544145 90-91 21.90277166122956 27.641030459942613 28.275530783924868 22.180667094902958 92-93 21.759669750391367 27.628643661356705 28.28202659084936 22.32965999740257 94-95 21.658202490616294 27.534133919290298 28.240597640203053 22.56706594989036 96-97 21.63090247924427 27.523870332073418 28.27691450321368 22.568312685468626 98-99 21.65151728698135 27.385618852686044 28.210662274363298 22.75220158596931 100 21.721947757195238 27.425648967179217 28.287282122928538 22.565121152697003 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1729.0 1 1249.0 2 1721.5 3 2464.0 4 1642.5 5 1249.5 6 1830.0 7 3192.5 8 4586.0 9 4961.5 10 4137.5 11 3188.5 12 3091.5 13 3267.5 14 3453.0 15 3653.0 16 3896.0 17 4145.5 18 4506.0 19 5008.5 20 5675.5 21 6707.5 22 8019.0 23 9818.5 24 12372.5 25 15383.5 26 19257.0 27 23978.0 28 29743.5 29 36715.0 30 44187.0 31 51711.5 32 59415.5 33 68362.5 34 77512.0 35 85501.5 36 95056.5 37 103852.0 38 110633.5 39 116530.0 40 119361.0 41 121854.5 42 124627.5 43 127300.0 44 130347.0 45 133628.5 46 135663.0 47 136056.0 48 138376.5 49 140389.5 50 138170.5 51 131973.5 52 124013.5 53 116530.0 54 109436.0 55 100005.5 56 88665.0 57 78815.5 58 69257.0 59 58166.5 60 46520.0 61 36586.5 62 29145.0 63 22508.5 64 16490.0 65 12069.5 66 8845.5 67 6618.0 68 5066.5 69 3824.5 70 2960.0 71 2235.0 72 1695.5 73 1332.0 74 1047.0 75 815.5 76 617.0 77 450.5 78 349.5 79 299.5 80 262.0 81 221.5 82 188.0 83 151.0 84 122.0 85 104.0 86 81.5 87 58.0 88 47.5 89 41.5 90 28.5 91 18.0 92 16.5 93 12.0 94 10.0 95 11.0 96 10.0 97 7.0 98 4.5 99 5.0 100 8.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15137137191031905 2 0.09626860314340965 3 0.030818291539355436 4 0.03351033520430368 5 0.029247932734802293 6 0.0022714118423000818 7 0.0041502339834618785 8 0.006477730068781716 9 0.006449687947271838 10-11 0.005257897783102042 12-13 0.008244383723904002 14-15 0.018760179290108085 16-17 0.009450194948828738 18-19 0.01720384154630988 20-21 0.023625487372071843 22-23 0.0429885722746423 24-25 0.0211157174969378 26-27 0.010067121622046043 28-29 0.02626144679400033 30-31 0.01620834623270923 32-33 0.021129738557692738 34-35 0.02089138052485878 36-37 0.022111212810538454 38-39 0.021031591132408167 40-41 0.026415678462304658 42-43 0.034463767335639515 44-45 0.03686136872473405 46-47 0.021634496744870536 48-49 0.01397899757267396 50-51 0.013446197263986288 52-53 0.023723634797356414 54-55 0.027453236958170125 56-57 0.020919422646368657 58-59 0.02673816285966825 60-61 0.02743921589741519 62-63 0.048891438852471526 64-65 0.03285134534882156 66-67 0.02387786646566074 68-69 0.031028607450679514 70-71 0.04446078365391087 72-73 0.04217535075085585 74-75 0.04203514014330646 76-77 0.02307866600262923 78-79 0.01787685246254694 80-81 0.02316279236715886 82-83 0.019138747930491435 84-85 0.020582917188250126 86-87 0.022461739329411923 88-89 0.018970495201432166 90-91 0.029556396071410945 92-93 0.02812624787440719 94-95 0.023611466311316904 96-97 0.016895378209701228 98-99 0.02065302249202482 100 0.043577456826349725 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3566064.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.24159848091362 #Duplication Level Percentage of deduplicated Percentage of total 1 81.00025631285507 57.705877370915104 2 13.063074671341079 18.612686413237476 3 3.2344688911255104 6.9128620212170855 4 1.107424183001265 3.155786759737198 5 0.5161310504761789 1.8385000530778053 6 0.28898473169550315 1.2352640533539354 7 0.17431258484111822 0.8692815025594794 8 0.1196431103460197 0.6818853142623024 9 0.0788029821154265 0.505264536986925 >10 0.3851046875743177 4.751227107616966 >50 0.020196270140800734 0.9993849152445368 >100 0.010928843470380346 1.4459195641525826 >500 2.686724078928615E-4 0.1270939278331124 >1k 2.6867240625897765E-4 0.3779002665250617 >5k 1.343362031294888E-4 0.7810661932803993 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9763 0.2737752323009346 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8485 0.23793740101131106 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6236 0.17487066973559645 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 2.8042121509877555E-5 7 0.0 0.0 0.0 0.0 2.8042121509877555E-5 8 0.0 0.0 0.0 0.0 2.8042121509877555E-5 9 0.0 0.0 0.0 0.0 8.412636452963268E-5 10-11 2.8042121509877555E-5 0.0 0.0 0.0 8.412636452963268E-5 12-13 2.8042121509877555E-5 0.0 0.0 1.4021060754938778E-5 8.412636452963268E-5 14-15 2.8042121509877555E-5 0.0 0.0 5.608424301975511E-5 1.1216848603951022E-4 16-17 2.8042121509877555E-5 0.0 0.0 5.608424301975511E-5 1.1216848603951022E-4 18-19 2.8042121509877555E-5 0.0 0.0 5.608424301975511E-5 1.1216848603951022E-4 20-21 2.8042121509877555E-5 0.0 0.0 1.402106075493878E-4 1.1216848603951022E-4 22-23 2.8042121509877555E-5 0.0 0.0 2.9444227585371434E-4 1.402106075493878E-4 24-25 2.8042121509877555E-5 0.0 0.0 5.328003086876737E-4 1.6825272905926535E-4 26-27 2.8042121509877555E-5 0.0 0.0 7.010530377469389E-4 1.6825272905926535E-4 28-29 2.8042121509877555E-5 0.0 0.0 0.0016685062298377146 1.6825272905926535E-4 30-31 2.8042121509877555E-5 0.0 0.0 0.004851287021208818 1.6825272905926535E-4 32-33 2.8042121509877555E-5 0.0 0.0 0.012100175431512166 1.6825272905926535E-4 34-35 5.608424301975511E-5 0.0 0.0 0.02250380251167674 2.2433697207902044E-4 36-37 5.608424301975511E-5 0.0 0.0 0.03886638041269029 2.2433697207902044E-4 38-39 5.608424301975511E-5 0.0 0.0 0.06608125933802647 2.2433697207902044E-4 40-41 5.608424301975511E-5 2.8042121509877555E-5 0.0 0.09959159454233014 2.2433697207902044E-4 42-43 5.608424301975511E-5 5.608424301975511E-5 0.0 0.13080247578282386 2.2433697207902044E-4 44-45 7.01053037746939E-5 5.608424301975511E-5 0.0 0.16735538117094925 2.5237909358889803E-4 46-47 8.412636452963268E-5 5.608424301975511E-5 0.0 0.2134285868116781 2.804212150987756E-4 48-49 8.412636452963268E-5 5.608424301975511E-5 0.0 0.2610581301962051 3.0846333660865315E-4 50-51 8.412636452963268E-5 5.608424301975511E-5 0.0 0.31554397228989717 3.0846333660865315E-4 52-53 8.412636452963268E-5 5.608424301975511E-5 0.0 0.37060467787454177 3.5052651887346946E-4 54-55 8.412636452963268E-5 5.608424301975511E-5 0.0 0.434456588552533 3.925897011382858E-4 56-57 8.412636452963268E-5 5.608424301975511E-5 0.0 0.509118737072582 4.066107618932246E-4 58-59 8.412636452963268E-5 5.608424301975511E-5 0.0 0.5986432099928661 4.206318226481633E-4 60-61 8.412636452963268E-5 5.608424301975511E-5 0.0 0.693032990995114 4.206318226481633E-4 62-63 8.412636452963268E-5 5.608424301975511E-5 0.0 0.7937883335801039 4.206318226481633E-4 64-65 8.412636452963268E-5 5.608424301975511E-5 0.0 0.8942492338892403 4.206318226481633E-4 66-67 8.412636452963268E-5 8.412636452963268E-5 0.0 1.009979069360505 4.206318226481633E-4 68-69 8.412636452963268E-5 8.412636452963268E-5 0.0 1.1489558235634583 4.206318226481633E-4 70-71 8.412636452963268E-5 8.412636452963268E-5 0.0 1.3026406704983422 4.206318226481633E-4 72-73 8.412636452963268E-5 8.412636452963268E-5 0.0 1.4530866523988353 4.206318226481633E-4 74-75 8.412636452963268E-5 8.412636452963268E-5 0.0 1.6087765110216754 4.206318226481633E-4 76-77 8.412636452963268E-5 8.412636452963268E-5 0.0 1.785904571538817 4.206318226481633E-4 78-79 8.412636452963268E-5 8.412636452963268E-5 0.0 1.994061239506638 4.206318226481633E-4 80-81 8.412636452963268E-5 8.412636452963268E-5 0.0 2.210616522866667 4.206318226481633E-4 82-83 8.412636452963268E-5 8.412636452963268E-5 0.0 2.4371127383019484 4.206318226481633E-4 84-85 8.412636452963268E-5 8.412636452963268E-5 0.0 2.666833797710866 4.206318226481633E-4 86-87 8.412636452963268E-5 8.412636452963268E-5 0.0 2.9064957891950343 4.206318226481633E-4 88 8.412636452963268E-5 8.412636452963268E-5 0.0 3.108525253612947 4.206318226481633E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 4120 0.0 45.077362 2 GGTATCA 5265 0.0 44.437263 1 GTACCTG 4530 0.0 42.606407 1 ACCTGGG 4860 0.0 37.415623 3 GTATCAA 8625 0.0 33.948498 1 TCAACGC 10080 0.0 28.386822 4 GTACATG 11060 0.0 28.219395 1 CAACGCA 10180 0.0 28.15294 5 ATCAACG 10245 0.0 27.839088 3 TACATGG 11210 0.0 27.136715 2 AACGCAG 10735 0.0 26.911356 6 ACATGGG 11015 0.0 26.703495 3 CATGGGG 6645 0.0 26.58599 4 TATCAAC 11165 0.0 25.772188 2 CCTGGGG 4395 0.0 25.443571 4 ATGGGGG 4520 0.0 24.115221 5 ACGCAGA 12310 0.0 23.316858 7 CGCAGAG 12490 0.0 22.868633 8 TATAACG 400 0.0 21.15783 2 GTATAAC 1170 0.0 19.715124 1 >>END_MODULE