FastQCFastQC Report
Mon 6 Jun 2016
ERR1211526_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211526_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3566064
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT117680.3299996859282391No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT107030.3001348265202195No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT80360.22534648845337607No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT37320.10465319747486304No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG42650.046.42162
GGTATCA62700.045.004821
GTACCTG44900.044.097211
ACCTGGG50800.038.5755923
GTATCAA99600.031.778331
GTACATG114400.025.817191
CCTGGGG43700.025.5935024
TCAACGC120300.025.5473754
ATCAACG121000.025.36113
CAACGCA122150.025.1604525
TACATGG117400.024.7558272
AACGCAG125900.024.5962856
ACATGGG112400.024.5421183
CATGGGG65400.024.4306664
TATCAAC129100.024.0058022
ACGCAGA143350.021.3727177
ATGGGGG45300.021.3699345
CTGGGGG29900.021.2176255
CGCAGAG147700.020.647818
TGGGGGG45750.020.2341486
Produced by FastQC (version 0.11.5)