##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211526_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 3566064 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12808575505095 33.0 31.0 34.0 30.0 34.0 2 32.372140825290856 34.0 31.0 34.0 31.0 34.0 3 32.394091637166355 34.0 31.0 34.0 30.0 34.0 4 36.018981992471254 37.0 35.0 37.0 35.0 37.0 5 35.942245007380684 37.0 35.0 37.0 35.0 37.0 6 35.96504970185617 37.0 35.0 37.0 35.0 37.0 7 35.89280366252541 37.0 35.0 37.0 35.0 37.0 8 35.892354147317604 37.0 35.0 37.0 35.0 37.0 9 37.56557678157206 39.0 37.0 39.0 35.0 39.0 10-11 37.52885674513974 39.0 37.0 39.0 35.0 39.0 12-13 37.44113986737198 39.0 37.0 39.0 34.0 39.0 14-15 38.69314880495695 40.0 38.0 41.0 34.0 41.0 16-17 38.624463694426126 40.0 38.0 41.0 34.0 41.0 18-19 38.6095496323117 40.0 38.0 41.0 34.0 41.0 20-21 38.54738123039856 40.0 38.0 41.0 34.0 41.0 22-23 38.43558388183723 40.0 38.0 41.0 34.0 41.0 24-25 38.32896591311878 40.0 38.0 41.0 34.0 41.0 26-27 38.160485762454066 40.0 38.0 41.0 34.0 41.0 28-29 38.1924086331597 40.0 38.0 41.0 34.0 41.0 30-31 38.11326367109508 40.0 37.5 41.0 33.5 41.0 32-33 37.933517598113774 40.0 37.0 41.0 33.0 41.0 34-35 37.825054037168144 40.0 37.0 41.0 33.0 41.0 36-37 37.76051986167383 40.0 37.0 41.0 33.0 41.0 38-39 37.66784149134732 40.0 37.0 41.0 33.0 41.0 40-41 37.511500915294846 40.0 37.0 41.0 32.0 41.0 42-43 37.3673537547279 40.0 36.5 41.0 31.5 41.0 44-45 37.19542428290687 40.0 36.0 41.0 31.0 41.0 46-47 36.94891328366513 39.0 36.0 41.0 30.5 41.0 48-49 36.738225954441646 39.0 36.0 41.0 30.0 41.0 50-51 36.55115149363556 39.0 35.0 41.0 30.0 41.0 52-53 36.3602850369483 39.0 35.0 40.0 29.0 41.0 54-55 36.16693587103316 39.0 35.0 40.0 28.5 41.0 56-57 35.97107272892466 38.5 35.0 40.0 28.0 41.0 58-59 35.716322533751494 38.0 34.0 40.0 28.0 41.0 60-61 35.40389109673859 38.0 34.0 40.0 27.0 41.0 62-63 35.08171291934188 37.5 34.0 40.0 26.0 41.0 64-65 34.7715513518546 37.0 33.5 40.0 26.0 41.0 66-67 34.43056364103393 36.5 33.0 39.0 26.0 41.0 68-69 34.03598995979881 36.0 32.5 39.0 25.5 40.0 70-71 33.64567167050283 35.5 32.0 39.0 25.0 40.0 72-73 33.229721339830135 35.0 32.0 38.0 24.5 40.0 74-75 32.81480758617905 35.0 31.0 37.0 23.5 39.0 76-77 31.308150666953818 33.5 29.5 35.5 21.5 38.5 78-79 32.07574443419972 34.0 31.0 36.0 23.0 39.0 80-81 32.016311401029256 34.0 31.0 36.0 23.0 37.5 82-83 31.713370399409545 34.0 31.0 35.5 22.5 37.0 84-85 31.40878122209809 34.0 30.5 35.0 21.5 37.0 86-87 31.09544079971644 34.0 30.0 35.0 20.0 36.0 88-89 30.8106594553547 34.0 30.0 35.0 20.0 36.0 90-91 30.524128002189528 34.0 29.5 35.0 19.5 35.5 92-93 30.302773169522474 34.0 29.0 35.0 19.0 35.0 94-95 30.08520963168356 34.0 29.0 35.0 18.5 35.0 96-97 29.874446588732003 34.0 29.0 35.0 18.0 35.0 98-99 29.696954962109487 34.0 29.0 35.0 18.0 35.0 100 27.692073950439475 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.29073906952908857 1101 2 0.1299846480751441 1101 3 0.23267151904897077 1101 4 0.10633762351570653 1101 5 0.12346110933406607 1101 6 0.14353643940366823 1101 7 0.10867281752616265 1101 8 0.08914334303889149 1101 9 0.12685180301451027 1101 10-11 0.11895095595748018 1101 12-13 0.09868441038254616 1101 14-15 0.2917933038459779 1101 16-17 0.2909737514809976 1101 18-19 0.2780888143930653 1101 20-21 0.2627987296062315 1101 22-23 0.2903079396883683 1101 24-25 0.2614205935615459 1101 26-27 0.16281639187997854 1101 28-29 0.21616252082052512 1101 30-31 0.30415368216366545 1101 32-33 0.2814644700066893 1101 34-35 0.2832637423805906 1101 36-37 0.33589748915527906 1101 38-39 0.2791539239981944 1101 40-41 0.32666948491393555 1101 42-43 0.2594711489043746 1101 44-45 0.30878417929253743 1101 46-47 0.32038901702735245 1101 48-49 0.3297291797502524 1101 50-51 0.29256889641898454 1101 52-53 0.31600078535424103 1101 54-55 0.2719365131128626 1101 56-57 0.2518278352193519 1101 58-59 0.2504063650390407 1101 60-61 0.3266577609287893 1101 62-63 0.3108031438979708 1101 64-65 0.2736068620899843 1101 66-67 0.24213931146221057 1101 68-69 0.20996930011364867 1101 70-71 0.2654404569861555 1101 72-73 0.12887524259003413 1101 74-75 0.20976219614555447 1101 76-77 0.26928739858740514 1101 78-79 0.26968390378142004 1101 80-81 0.2839022512730338 1101 82-83 0.312691482331914 1101 84-85 0.3588266148516084 1101 86-87 0.36219726552345577 1101 88-89 0.324871614845204 1101 90-91 0.19075450023425944 1101 92-93 0.15702040206197765 1101 94-95 0.2826650398090287 1101 96-97 0.12348288809756625 1101 98-99 0.1411855342245545 1101 100 0.24165712616388646 1102 1 0.24688026854717293 1102 2 0.22532424434180598 1102 3 0.2606492862750116 1102 4 0.1387972542925766 1102 5 0.15194823083650988 1102 6 0.13363096274913744 1102 7 0.10081517053779976 1102 8 0.15302706370512453 1102 9 0.15925647426139733 1102 10-11 0.1256642782023576 1102 12-13 0.1449194672817029 1102 14-15 0.1912705228467928 1102 16-17 0.2560885421317707 1102 18-19 0.23811151064341374 1102 20-21 0.2565441080538733 1102 22-23 0.2633429701255352 1102 24-25 0.2123752022352079 1102 26-27 0.1791558523136203 1102 28-29 0.22401496152863132 1102 30-31 0.24329398828511017 1102 32-33 0.2869154845854567 1102 34-35 0.2216634649210789 1102 36-37 0.2542865016335156 1102 38-39 0.29858697182120864 1102 40-41 0.3261661981069963 1102 42-43 0.15764425341712496 1102 44-45 0.3185504955987213 1102 46-47 0.21631073805919243 1102 48-49 0.2606658365432608 1102 50-51 0.25959141690843524 1102 52-53 0.2491820926942765 1102 54-55 0.21396168567365237 1102 56-57 0.25443242371378005 1102 58-59 0.3398219972673999 1102 60-61 0.3654105319029597 1102 62-63 0.32283857635392366 1102 64-65 0.321953124352639 1102 66-67 0.2885459015952492 1102 68-69 0.3222967931487801 1102 70-71 0.25638274282502493 1102 72-73 0.3483918649119815 1102 74-75 0.21856018346807105 1102 76-77 0.10230864216811852 1102 78-79 0.3451970061881724 1102 80-81 0.29272536966267637 1102 82-83 0.18017583511103297 1102 84-85 0.13127732840559148 1102 86-87 0.14513260547985496 1102 88-89 0.14675639007677788 1102 90-91 0.1933860596091428 1102 92-93 0.18924175744071192 1102 94-95 0.23824368654411998 1102 96-97 0.12444166570993431 1102 98-99 0.25170918815644683 1102 100 0.30574933291036643 1103 1 0.2461889340620047 1103 2 0.2147790838190673 1103 3 0.262291564736465 1103 4 0.12481622736779485 1103 5 0.10843204386644345 1103 6 0.16745689939867958 1103 7 0.08573747983565028 1103 8 0.08648435332458604 1103 9 0.18655731577966606 1103 10-11 0.1522898167426021 1103 12-13 0.19405840455192447 1103 14-15 0.25954191879512933 1103 16-17 0.26196527342779774 1103 18-19 0.28629853350714285 1103 20-21 0.2278349393903767 1103 22-23 0.2644239905678418 1103 24-25 0.19870017136582163 1103 26-27 0.2870619084580497 1103 28-29 0.31584545298942146 1103 30-31 0.4218741479558119 1103 32-33 0.4728636162549762 1103 34-35 0.3541434924968243 1103 36-37 0.3126943672421234 1103 38-39 0.4351209522115198 1103 40-41 0.38172922813172505 1103 42-43 0.418841251915282 1103 44-45 0.3675956116757604 1103 46-47 0.4771488326466482 1103 48-49 0.40781962294845897 1103 50-51 0.5037737848125019 1103 52-53 0.5186216537182275 1103 54-55 0.46757873139682715 1103 56-57 0.6031422755601454 1103 58-59 0.4758657163131019 1103 60-61 0.5458109469365482 1103 62-63 0.5474897552131424 1103 64-65 0.5521652953331184 1103 66-67 0.5426320532843647 1103 68-69 0.5540977912944811 1103 70-71 0.622710405869924 1103 72-73 0.5190634841369004 1103 74-75 0.49751562791152537 1103 76-77 0.500879314913039 1103 78-79 0.5273629443205294 1103 80-81 0.6048661503621346 1103 82-83 0.4442265583338525 1103 84-85 0.28511749544380294 1103 86-87 0.42954955353893354 1103 88-89 0.5259552572353634 1103 90-91 0.4295728200685396 1103 92-93 0.46080329999000114 1103 94-95 0.5670446967231619 1103 96-97 0.6505445693535883 1103 98-99 0.6101725235561517 1103 100 0.6630142258117466 1104 1 0.15183403853611566 1104 2 0.142122815593261 1104 3 0.196516505974067 1104 4 0.06882974797092345 1104 5 0.1320813185779386 1104 6 0.05376449079477652 1104 7 0.15719164454305457 1104 8 0.0726913974031902 1104 9 0.11082285649411716 1104 10-11 0.1044341609308006 1104 12-13 0.18309430590441877 1104 14-15 0.27380087390443464 1104 16-17 0.19422290366556183 1104 18-19 0.20934809729701698 1104 20-21 0.16773057727370855 1104 22-23 0.28370213476802775 1104 24-25 0.2933940352822759 1104 26-27 0.33006846003848267 1104 28-29 0.3374516942550869 1104 30-31 0.2701819760523776 1104 32-33 0.3261056878939925 1104 34-35 0.39665986330987124 1104 36-37 0.3661616220895496 1104 38-39 0.431091657778083 1104 40-41 0.5012450183543535 1104 42-43 0.6366266408624242 1104 44-45 0.4198794804773627 1104 46-47 0.47650472682526157 1104 48-49 0.4200310105657721 1104 50-51 0.5121732796965119 1104 52-53 0.34687690703074026 1104 54-55 0.39908944534789015 1104 56-57 0.5504713852292653 1104 58-59 0.5299157704775155 1104 60-61 0.42367564061367347 1104 62-63 0.4226823456051605 1104 64-65 0.49671704984235276 1104 66-67 0.5157202029695824 1104 68-69 0.43746716577359734 1104 70-71 0.3966143428403228 1104 72-73 0.4839074978357658 1104 74-75 0.4770304198689459 1104 76-77 0.5362049597958496 1104 78-79 0.6382821830379086 1104 80-81 0.741781129556287 1104 82-83 0.5699156655321147 1104 84-85 0.6234032214217073 1104 86-87 0.6141598305707063 1104 88-89 0.7066467620527277 1104 90-91 0.4916702636183068 1104 92-93 0.47555042073506115 1104 94-95 0.6372864497388626 1104 96-97 0.5954182411396438 1104 98-99 0.5686525190732574 1104 100 0.7500592313738963 1105 1 0.1400112271887224 1105 2 0.08520102939146312 1105 3 0.152650561068306 1105 4 0.05038807879451923 1105 5 0.010360435098782261 1105 6 0.04650027232651155 1105 7 0.08244812650620048 1105 8 0.05019177875266223 1105 9 0.10798718095798421 1105 10-11 0.08209637134965675 1105 12-13 0.13337904684101431 1105 14-15 0.19619576812395678 1105 16-17 0.25667621280113195 1105 18-19 0.20531654893995466 1105 20-21 0.1360448366148148 1105 22-23 0.19693339681965227 1105 24-25 0.21479644306415224 1105 26-27 0.2707422165458695 1105 28-29 0.33096288637307936 1105 30-31 0.3442732887159181 1105 32-33 0.34399928943660285 1105 34-35 0.2843764013607242 1105 36-37 0.3056879078239447 1105 38-39 0.29450107847890905 1105 40-41 0.30503973891319447 1105 42-43 0.25705338069604267 1105 44-45 0.21404121567611156 1105 46-47 0.32835388062056836 1105 48-49 0.40867853022971445 1105 50-51 0.44402564073863715 1105 52-53 0.38492629472037976 1105 54-55 0.4471492565896824 1105 56-57 0.4645724174151553 1105 58-59 0.48090111883536935 1105 60-61 0.4593220377412379 1105 62-63 0.5000293071299282 1105 64-65 0.5048041031256716 1105 66-67 0.556323165370479 1105 68-69 0.45779993718686285 1105 70-71 0.5582957615385737 1105 72-73 0.4102239357579336 1105 74-75 0.5243509998330396 1105 76-77 0.464428633970865 1105 78-79 0.6188866512802917 1105 80-81 0.5133298190830686 1105 82-83 0.5650148359503504 1105 84-85 0.6068370093757629 1105 86-87 0.6548067948333696 1105 88-89 0.63854165822789 1105 90-91 0.6975065727812542 1105 92-93 0.7509859413846449 1105 94-95 0.7211544871776034 1105 96-97 0.7659054101967548 1105 98-99 0.802646108490908 1105 100 1.0271340658291166 1106 1 0.13331677567684608 1106 2 0.12445182707544689 1106 3 0.1316194214852615 1106 4 0.1168240194774981 1106 5 0.10951616221981197 1106 6 0.06902147041667916 1106 7 0.1082055541923097 1106 8 0.07717009399242158 1106 9 0.12185854167402255 1106 10-11 0.13418516322280283 1106 12-13 0.1400368506643872 1106 14-15 0.1483269365196591 1106 16-17 0.15283309186713012 1106 18-19 0.14478748549760923 1106 20-21 0.17381519405474677 1106 22-23 0.22782852152568722 1106 24-25 0.19085883287536376 1106 26-27 0.2547238067691069 1106 28-29 0.18274713735892334 1106 30-31 0.20858767926626598 1106 32-33 0.270762534697532 1106 34-35 0.21315793732853905 1106 36-37 0.2404100200563022 1106 38-39 0.19880666235100364 1106 40-41 0.2630580565792897 1106 42-43 0.23810463017159833 1106 44-45 0.3951416657265767 1106 46-47 0.355592731438918 1106 48-49 0.37911493820582365 1106 50-51 0.34989886107914003 1106 52-53 0.40308929633737733 1106 54-55 0.33741301884980146 1106 56-57 0.3159516482574034 1106 58-59 0.4507075656833024 1106 60-61 0.44188489905796047 1106 62-63 0.47357901347101716 1106 64-65 0.555599554543754 1106 66-67 0.4442343145240457 1106 68-69 0.457323517236361 1106 70-71 0.5742356806721531 1106 72-73 0.5348158265371481 1106 74-75 0.47273322202530466 1106 76-77 0.4704246030059558 1106 78-79 0.5276804536928097 1106 80-81 0.7071929319630073 1106 82-83 0.6679576312956144 1106 84-85 0.6542047786183254 1106 86-87 0.5900017464949272 1106 88-89 0.5221432638740495 1106 90-91 0.6518029034630395 1106 92-93 0.7757866678232723 1106 94-95 0.7849706797832496 1106 96-97 0.8745491677053145 1106 98-99 0.8680173706373004 1106 100 0.9159733422661418 1107 1 0.24569966246324526 1107 2 0.18087860736596184 1107 3 0.1741418743633929 1107 4 0.1268521854140161 1107 5 0.1578847919493498 1107 6 0.15032140191240018 1107 7 0.1799310261145166 1107 8 0.1943139317385345 1107 9 0.22583703193678417 1107 10-11 0.24453099258672495 1107 12-13 0.21491316084669876 1107 14-15 0.29060360994294854 1107 16-17 0.2809980351669381 1107 18-19 0.23094637901138526 1107 20-21 0.23728490691338777 1107 22-23 0.2893575326610218 1107 24-25 0.35398675544649194 1107 26-27 0.4219541922112171 1107 28-29 0.38292772325123536 1107 30-31 0.2617547814303478 1107 32-33 0.26153404519347845 1107 34-35 0.23651671077359282 1107 36-37 0.4116200269232806 1107 38-39 0.3476976682603592 1107 40-41 0.3502609989250587 1107 42-43 0.443831014196725 1107 44-45 0.42935256014197165 1107 46-47 0.4916453233037714 1107 48-49 0.5307767394959555 1107 50-51 0.5066513746730905 1107 52-53 0.35388057182197485 1107 54-55 0.41713470331020375 1107 56-57 0.4994393852186221 1107 58-59 0.589733803402261 1107 60-61 0.4830689940358468 1107 62-63 0.5361434517759207 1107 64-65 0.6314687353699426 1107 66-67 0.7964583309716957 1107 68-69 0.6659080858125179 1107 70-71 0.7534228634467013 1107 72-73 0.6939729562657178 1107 74-75 0.6607486422478956 1107 76-77 0.6120249932292943 1107 78-79 0.6313489779483703 1107 80-81 0.693741690153928 1107 82-83 0.7332120282744725 1107 84-85 0.5728327010514995 1107 86-87 0.5769725523044684 1107 88-89 0.5204894221026599 1107 90-91 0.5458470866550158 1107 92-93 0.6679357406427968 1107 94-95 0.7597683190203384 1107 96-97 0.8390870286667322 1107 98-99 0.9821512825738452 1107 100 1.0694299667514962 1108 1 0.1833222540715269 1108 2 0.15179882859467853 1108 3 0.20062879689920976 1108 4 0.1747604962646463 1108 5 0.09888930304072119 1108 6 0.13471641704730075 1108 7 0.1361482289244833 1108 8 0.18744936129392897 1108 9 0.15909976929232528 1108 10-11 0.181637055558447 1108 12-13 0.17763621811421615 1108 14-15 0.18958742519247096 1108 16-17 0.1758161840693262 1108 18-19 0.16914446914255876 1108 20-21 0.27591570869677895 1108 22-23 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0.6089686649824806 1108 90-91 0.7222975779728031 1108 92-93 0.7101178866597913 1108 94-95 0.8694728735941375 1108 96-97 0.9096769205986455 1108 98-99 0.9094117529115024 1108 100 1.0921859072505313 1109 1 0.10715597717716463 1109 2 0.04679363304126838 1109 3 0.1402502733956439 1109 4 0.023432138699973848 1109 5 0.016746587974012073 1109 6 0.03806304185611964 1109 7 0.0389039882582054 1109 8 0.12744290599868435 1109 9 0.08010217676760334 1109 10-11 0.06585951552813896 1109 12-13 0.04733080760060915 1109 14-15 0.019609527807396887 1109 16-17 0.09547152829484418 1109 18-19 0.14401568769520878 1109 20-21 0.15552840226747122 1109 22-23 0.17799114326945187 1109 24-25 0.14703417504311744 1109 26-27 0.08433390682915132 1109 28-29 0.19039136079228314 1109 30-31 0.17356502850471855 1109 32-33 0.1874323309790853 1109 34-35 0.1601031411102838 1109 36-37 0.19873801565758953 1109 38-39 0.23198816067986172 1109 40-41 0.07505117198293476 1109 42-43 0.143944950544018 1109 44-45 0.16708412587927768 1109 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-0.27770817385806623 2316 82-83 -0.21017684029875028 2316 84-85 -0.4104501333326205 2316 86-87 -0.3278969231485789 2316 88-89 -0.24582294926922188 2316 90-91 -0.1013059262494238 2316 92-93 -0.05337896521714569 2316 94-95 -0.051672046509342096 2316 96-97 0.02290653682438304 2316 98-99 -0.31436101130827154 2316 100 -0.38574255681377423 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 5.0 11 8.0 12 29.0 13 89.0 14 268.0 15 814.0 16 1770.0 17 3837.0 18 7027.0 19 10803.0 20 14586.0 21 20321.0 22 26535.0 23 31794.0 24 34388.0 25 37593.0 26 43993.0 27 52238.0 28 63697.0 29 76929.0 30 93563.0 31 114115.0 32 140993.0 33 178039.0 34 238571.0 35 326678.0 36 479191.0 37 736527.0 38 718239.0 39 113410.0 40 12.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.184387648337754 20.900336601949615 13.627625645237867 23.28765010447476 2 15.5291579098003 21.84512903108138 40.03956373268385 22.586149326434466 3 17.406091184084076 27.416862238466827 31.057315974390633 24.119730603058464 4 11.779672257659984 17.537779375569436 38.709877334281536 31.972671032489043 5 12.05656097938526 38.54231865915571 35.74517273173916 13.655947629719863 6 30.14314754092844 38.331668087944024 18.53290525824016 12.992279112887372 7 26.70599854629642 32.97119737615478 23.17689755427833 17.145906523270472 8 25.717050693053427 36.73720166762431 20.60955162756577 16.936196011756486 9 26.802998266994948 15.185134293870547 20.361883984004756 37.64998345512975 10-11 24.438190116610357 26.572237626694307 29.707571148470695 19.28200110822464 12-13 24.857714275458882 24.405002265803418 29.5617941798016 21.175489278936105 14-15 22.14339114497104 25.984657033637088 26.963481306000116 24.90847051539176 16-17 21.834321169340594 28.745691303865012 27.670241870262956 21.749745656531438 18-19 21.610851064545763 27.94219506969659 29.488789768336087 20.95816409742156 20-21 22.312258891693652 27.34504885017462 29.406460458202705 20.936231799929022 22-23 22.043547171335117 27.12993933928275 29.274951318877058 21.551562170505072 24-25 21.807480686684446 27.204132400352993 29.3667414176362 21.62164549532636 26-27 21.75885141407682 27.398080988386464 29.271608159662172 21.571459437874545 28-29 21.780848001224896 27.55430510691364 29.34780871276545 21.317038179096016 30-31 22.269486561398274 27.342963929292125 29.35738009801469 21.030169411294906 32-33 22.03289826619065 27.42904230918829 29.249644485935494 21.28841493868557 34-35 22.09892879231875 27.593430434738103 29.23419524536176 21.073445527581384 36-37 22.07408790835611 27.592830719845836 29.128562216622143 21.204519155175916 38-39 22.046808552403736 27.78500651287221 28.987721588383675 21.18046334634038 40-41 22.132484549778983 27.458451787949002 29.219359836786772 21.18970382548524 42-43 21.832147962144028 27.577218325826436 29.04083227724477 21.549801434784765 44-45 21.778196312105585 27.784492648805358 29.002381738267914 21.434929300821143 46-47 21.774005383294735 27.624860972683624 28.79702320290893 21.804110441112712 48-49 21.75026098759529 27.70966821448027 28.847987783887547 21.692083014036893 50-51 21.899648759018177 27.87405467883584 28.7006888103617 21.525607751784285 52-53 21.915259163870637 27.88959481558684 28.5175114020381 21.677634618504428 54-55 21.812213034464328 27.83059172843796 28.76722143749104 21.58997379960667 56-57 21.949237337377536 27.887999982052357 28.42090474064535 21.741857939924756 58-59 21.88244815683226 27.930698541938515 28.614719598059285 21.572133703169943 60-61 22.168062933087743 28.01742040187682 28.525634643822357 21.28888202121308 62-63 21.995442562261612 28.001977226834196 28.561448283598835 21.441131927305364 64-65 21.89929937048786 27.66197991214649 28.594582179826 21.844138537539653 66-67 21.809653683144088 27.861978062041864 28.42954648231958 21.898821772494472 68-69 21.671463816366064 27.90052273647031 28.581757257213887 21.846256189949738 70-71 21.749846707252146 27.75287905023417 28.568986784363343 21.928287458150344 72-73 21.664599582844023 28.08059874152104 28.23328484041155 22.02151683522339 74-75 21.740253982346673 28.105600839494254 28.102544156105104 22.051601022053973 76-77 21.571514783726514 27.8813235638882 28.393094907721594 22.15406674466369 78-79 21.608108691738128 27.850042375329316 28.36739438559138 22.17445454734118 80-81 21.670927993255916 28.060828579128728 28.084568052362012 22.183675375253344 82-83 21.707346146025426 28.08550052938248 28.17745181217089 22.029701512421205 84-85 21.60803547951689 28.16707627044367 28.06958472838186 22.15530352165758 86-87 21.558846071050173 28.173946213464657 28.113418305204547 22.153789410280627 88-89 21.411572563138012 28.023660417281377 28.20428713589973 22.36047988368088 90-91 21.779663869317417 27.98027083426828 28.159849997756137 22.08021529865817 92-93 21.602389027997333 27.99372896981162 28.09364581330333 22.310236188887718 94-95 21.405183265716882 27.923797368042724 28.03910416673972 22.63191519950068 96-97 21.33743580538127 27.9564023526574 28.078018023577062 22.628143818384274 98-99 21.324648596588013 27.798094454926098 28.01624125049888 22.861015697987007 100 20.119860876569607 28.61249311801231 28.321507393000694 22.946138612417393 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1730.0 1 1255.0 2 1828.0 3 2615.0 4 1692.5 5 1180.5 6 1745.0 7 3315.0 8 5013.5 9 5771.0 10 4877.5 11 3709.0 12 3738.5 13 3907.5 14 4166.5 15 4407.0 16 4591.5 17 4896.5 18 5249.0 19 5678.0 20 6373.5 21 7355.5 22 8652.0 23 10600.0 24 13283.0 25 16587.0 26 20916.5 27 25912.5 28 31557.5 29 38289.5 30 45859.0 31 53485.0 32 61404.0 33 70197.0 34 79132.0 35 87069.5 36 96262.5 37 105014.0 38 111883.0 39 118164.5 40 121218.5 41 123101.5 42 125264.5 43 128224.0 44 130985.5 45 134017.5 46 136097.0 47 136714.5 48 138746.0 49 139936.5 50 138353.0 51 132622.5 52 123626.0 53 115091.5 54 107116.0 55 96961.5 56 85779.0 57 75645.0 58 65839.5 59 54807.0 60 43218.5 61 33739.0 62 26605.0 63 20077.0 64 14609.0 65 10676.0 66 7646.0 67 5563.5 68 4120.0 69 3047.5 70 2271.5 71 1696.0 72 1245.0 73 876.5 74 646.5 75 476.0 76 330.0 77 191.5 78 106.0 79 76.0 80 58.5 81 36.0 82 29.0 83 25.0 84 17.5 85 11.5 86 6.0 87 4.0 88 5.5 89 3.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.5 95 1.0 96 1.0 97 0.5 98 1.0 99 1.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00367351791779396 2 0.061524414592671356 3 7.010530377469389E-4 4 8.693057668062044E-4 5 0.0020190327487111842 6 6.449687947271838E-4 7 0.0 8 9.253900098259594E-4 9 1.1216848603951022E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 5.608424301975512E-4 18-19 0.0010095163743555921 20-21 0.0030706123053315924 22-23 0.0 24-25 2.8042121509877555E-5 26-27 2.664001543438368E-4 28-29 0.0015423166830432656 30-31 0.0019068642626716738 32-33 0.002832254272497633 34-35 0.003799707464588409 36-37 0.0012198322856796736 38-39 0.0013740639539840003 40-41 0.004949434446493389 42-43 0.006533814311801471 44-45 0.005075623993287838 46-47 0.006113182489153307 48-49 0.007893857205030533 50-51 0.007809730840500899 52-53 0.004052086558177307 54-55 0.0024396645713593472 56-57 0.0038137285253433476 58-59 0.004626950049129797 60-61 0.006870319769920001 62-63 0.013011544380583186 64-65 0.003589391553264327 66-67 0.009155752672975023 68-69 0.01135705921150041 70-71 0.004893350203473633 72-73 0.007052593559734205 74-75 0.0030145280623118374 76-77 0.0032528860951457966 78-79 0.008693057668062042 80-81 0.007655499172196573 82-83 0.01651680956931788 84-85 0.017568389125938288 86-87 0.02149428613732115 88-89 0.01962948505691429 90-91 0.021985023263744005 92-93 0.030832312600110375 94-95 0.03439366203186482 96-97 0.020582917188250126 98-99 0.016124219868179596 100 0.0167411465413969 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 3566064.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 69.49069080820549 #Duplication Level Percentage of deduplicated Percentage of total 1 79.65370269206194 55.351908255028015 2 13.956389811932402 19.396783384395672 3 3.4688715109869457 7.231628328701592 4 1.19261180260896 3.315016721172633 5 0.5436076842660746 1.888783675414919 6 0.302784653455196 1.2624428840834803 7 0.18910774567295507 0.9198859518797255 8 0.1317376454373741 0.7323631989511651 9 0.0922481477997541 0.5769348764744118 >10 0.43349412050268343 5.13125719167606 >50 0.02258354230164167 1.086551909756691 >100 0.012031828540787541 1.5580050728280213 >500 4.604524631396547E-4 0.21825262658103692 >1k 2.3022623122649624E-4 0.35404857208268803 >5k 4.604524624529925E-5 0.25712917535078905 >10k+ 9.20904924905985E-5 0.7190081756231436 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11768 0.3299996859282391 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10703 0.3001348265202195 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8036 0.22534648845337607 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 3732 0.10465319747486304 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.8042121509877555E-5 2 0.0 0.0 0.0 0.0 2.8042121509877555E-5 3 0.0 0.0 0.0 0.0 2.8042121509877555E-5 4 0.0 0.0 0.0 0.0 2.8042121509877555E-5 5 0.0 0.0 0.0 2.8042121509877555E-5 2.8042121509877555E-5 6 2.8042121509877555E-5 0.0 0.0 2.8042121509877555E-5 2.8042121509877555E-5 7 2.8042121509877555E-5 0.0 0.0 5.608424301975511E-5 2.8042121509877555E-5 8 2.8042121509877555E-5 0.0 0.0 5.608424301975511E-5 2.8042121509877555E-5 9 2.8042121509877555E-5 0.0 0.0 5.608424301975511E-5 2.8042121509877555E-5 10-11 2.8042121509877555E-5 0.0 0.0 7.01053037746939E-5 2.8042121509877555E-5 12-13 2.8042121509877555E-5 0.0 0.0 8.412636452963268E-5 2.8042121509877555E-5 14-15 2.8042121509877555E-5 0.0 0.0 8.412636452963268E-5 2.8042121509877555E-5 16-17 5.608424301975511E-5 0.0 0.0 1.1216848603951022E-4 2.8042121509877555E-5 18-19 5.608424301975511E-5 0.0 0.0 1.402106075493878E-4 2.8042121509877555E-5 20-21 5.608424301975511E-5 0.0 0.0 2.5237909358889803E-4 2.8042121509877555E-5 22-23 5.608424301975511E-5 0.0 0.0 4.3465288340310213E-4 5.608424301975512E-5 24-25 5.608424301975511E-5 0.0 0.0 7.010530377469389E-4 8.412636452963268E-5 26-27 5.608424301975511E-5 0.0 0.0 8.693057668062043E-4 8.412636452963268E-5 28-29 5.608424301975511E-5 0.0 0.0 0.001892843201916735 8.412636452963268E-5 30-31 5.608424301975511E-5 0.0 0.0 0.005103666114797715 8.412636452963268E-5 32-33 5.608424301975511E-5 0.0 0.0 0.012422659828875759 8.412636452963268E-5 34-35 5.608424301975511E-5 2.8042121509877555E-5 0.0 0.022770202666020575 8.412636452963268E-5 36-37 5.608424301975511E-5 2.8042121509877555E-5 0.0 0.039034633141749556 8.412636452963268E-5 38-39 5.608424301975511E-5 2.8042121509877555E-5 0.0 0.06664210176822402 8.412636452963268E-5 40-41 7.01053037746939E-5 2.8042121509877555E-5 0.0 0.10012439485101782 8.412636452963268E-5 42-43 8.412636452963268E-5 2.8042121509877555E-5 0.0 0.13130723397000166 8.412636452963268E-5 44-45 8.412636452963268E-5 2.8042121509877555E-5 0.0 0.16784611829737212 9.814742528457146E-5 46-47 8.412636452963268E-5 2.8042121509877555E-5 0.0 0.2140455134848954 1.402106075493878E-4 48-49 8.412636452963268E-5 2.8042121509877555E-5 0.0 0.26171712005168724 1.962948505691429E-4 50-51 8.412636452963268E-5 2.8042121509877555E-5 0.0 0.31630110957066393 1.962948505691429E-4 52-53 8.412636452963268E-5 2.8042121509877555E-5 0.0 0.3712075834870041 2.1031591132408166E-4 54-55 8.412636452963268E-5 4.206318226481633E-5 0.0 0.43458277809932744 2.2433697207902044E-4 56-57 8.412636452963268E-5 5.608424301975511E-5 0.0 0.5094131793484357 2.2433697207902044E-4 58-59 1.1216848603951022E-4 5.608424301975511E-5 0.0 0.5987693995396606 2.2433697207902044E-4 60-61 1.1216848603951022E-4 5.608424301975511E-5 0.0 0.6927806119015251 2.5237909358889803E-4 62-63 1.1216848603951022E-4 5.608424301975511E-5 0.0 0.7932555332714163 2.5237909358889803E-4 64-65 1.1216848603951022E-4 5.608424301975511E-5 0.0 0.8925807276594027 2.5237909358889803E-4 66-67 1.2618954679444901E-4 5.608424301975511E-5 0.0 1.0068523728121537 2.5237909358889803E-4 68-69 1.402106075493878E-4 5.608424301975511E-5 0.0 1.1438942206309253 2.5237909358889803E-4 70-71 1.402106075493878E-4 7.01053037746939E-5 0.0 1.2962751089156 2.5237909358889803E-4 72-73 1.402106075493878E-4 8.412636452963268E-5 0.0 1.4450946477685203 3.0846333660865315E-4 74-75 1.402106075493878E-4 8.412636452963268E-5 0.0 1.5997749900170048 3.0846333660865315E-4 76-77 1.402106075493878E-4 8.412636452963268E-5 0.0 1.7743091542944827 3.0846333660865315E-4 78-79 1.402106075493878E-4 8.412636452963268E-5 0.0 1.9802645157237784 3.0846333660865315E-4 80-81 1.402106075493878E-4 8.412636452963268E-5 0.0 2.193749186778476 3.0846333660865315E-4 82-83 1.402106075493878E-4 8.412636452963268E-5 0.0 2.4162914630808645 3.365054581185307E-4 84-85 1.402106075493878E-4 1.1216848603951022E-4 0.0 2.642605404726331 3.365054581185307E-4 86-87 1.402106075493878E-4 1.1216848603951022E-4 0.0 2.878061077984018 3.365054581185307E-4 88 1.402106075493878E-4 1.1216848603951022E-4 0.0 3.077342414493963 3.365054581185307E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TACCTGG 4265 0.0 46.4216 2 GGTATCA 6270 0.0 45.00482 1 GTACCTG 4490 0.0 44.09721 1 ACCTGGG 5080 0.0 38.575592 3 GTATCAA 9960 0.0 31.77833 1 GTACATG 11440 0.0 25.81719 1 CCTGGGG 4370 0.0 25.593502 4 TCAACGC 12030 0.0 25.547375 4 ATCAACG 12100 0.0 25.3611 3 CAACGCA 12215 0.0 25.160452 5 TACATGG 11740 0.0 24.755827 2 AACGCAG 12590 0.0 24.596285 6 ACATGGG 11240 0.0 24.542118 3 CATGGGG 6540 0.0 24.430666 4 TATCAAC 12910 0.0 24.005802 2 ACGCAGA 14335 0.0 21.372717 7 ATGGGGG 4530 0.0 21.369934 5 CTGGGGG 2990 0.0 21.217625 5 CGCAGAG 14770 0.0 20.64781 8 TGGGGGG 4575 0.0 20.234148 6 >>END_MODULE