FastQCFastQC Report
Mon 6 Jun 2016
ERR1211525_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211525_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9938103
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT296560.2984070501181161No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT256130.2577252419299739No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT171520.17258826961242No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151050.050.059611
GTATCAA256800.036.7422141
TCAACGC305150.029.9822434
ATCAACG306250.029.8598063
CAACGCA306700.029.7679355
GTACATG317000.029.2745341
AACGCAG320050.028.5643986
TATCAAC329950.027.9187012
TACCTGG84700.027.4253652
TACATGG330550.027.1851942
ACATGGG325250.026.3528613
GTACCTG92900.025.644691
ACGCAGA364100.024.760717
CGCAGAG370450.024.0578888
CATGGGG183100.023.4568334
GCAGAGT419750.020.618139
ACCTGGG110150.020.6054883
GAGTACT257500.020.08088312-13
ATGGGGG113350.019.7736365
CAGAGTA412250.019.02701210-11
Produced by FastQC (version 0.11.5)