FastQCFastQC Report
Mon 6 Jun 2016
ERR1211525_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211525_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9938103
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327300.3293385065540174No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT289650.29145401290366985No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT205750.2070314626443296No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA158750.047.8979871
GTATCAA270750.036.5077671
TCAACGC333600.029.0609664
GTACATG335350.029.0123711
ATCAACG336250.028.7343963
CAACGCA337700.028.7219095
AACGCAG350150.027.8211756
TACATGG345350.027.4499242
TATCAAC356100.027.2947162
ACATGGG339650.026.8279463
TACCTGG79700.026.3139842
CATGGGG185750.024.6161924
GTACCTG89750.024.2063061
ACGCAGA401800.024.0810017
CGCAGAG410600.023.3703338
ATGGGGG108650.021.2799225
GCAGAGT460300.020.4383989
ACCTGGG103800.019.6939643
GAGTACT281300.019.3372512-13
AGAGTAC424000.019.31808310-11
Produced by FastQC (version 0.11.5)