##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211525_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9938103 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.12534716132445 33.0 31.0 34.0 30.0 34.0 2 32.34233676185485 34.0 31.0 34.0 30.0 34.0 3 32.35818032878105 34.0 31.0 34.0 30.0 34.0 4 35.99180095034233 37.0 35.0 37.0 35.0 37.0 5 35.915891996691926 37.0 35.0 37.0 35.0 37.0 6 35.933087632519005 37.0 35.0 37.0 35.0 37.0 7 35.85658128115597 37.0 35.0 37.0 35.0 37.0 8 35.85533808615185 37.0 35.0 37.0 35.0 37.0 9 37.509168198397624 39.0 37.0 39.0 35.0 39.0 10-11 37.47358580405134 39.0 37.0 39.0 34.5 39.0 12-13 37.389248833504745 39.0 37.0 39.0 34.0 39.0 14-15 38.63878000660689 40.0 38.0 41.0 34.0 41.0 16-17 38.56941259312768 40.0 38.0 41.0 34.0 41.0 18-19 38.55245930737486 40.0 38.0 41.0 34.0 41.0 20-21 38.49218024808155 40.0 38.0 41.0 34.0 41.0 22-23 38.386858186114594 40.0 38.0 41.0 34.0 41.0 24-25 38.28059907408889 40.0 38.0 41.0 34.0 41.0 26-27 38.10491982222362 40.0 38.0 41.0 33.0 41.0 28-29 38.1456025863286 40.0 38.0 41.0 33.0 41.0 30-31 38.07663459515362 40.0 37.5 41.0 33.0 41.0 32-33 37.90695699169147 40.0 37.0 41.0 33.0 41.0 34-35 37.79940955532459 40.0 37.0 41.0 33.0 41.0 36-37 37.74928550247466 40.0 37.0 41.0 33.0 41.0 38-39 37.65923567103299 40.0 37.0 41.0 32.5 41.0 40-41 37.503502831475984 40.0 37.0 41.0 32.0 41.0 42-43 37.359404908562524 40.0 36.5 41.0 31.0 41.0 44-45 37.179988374038786 40.0 36.0 41.0 31.0 41.0 46-47 36.93329773297781 39.0 36.0 41.0 30.5 41.0 48-49 36.72068024450944 39.0 36.0 41.0 30.0 41.0 50-51 36.52800594841893 39.0 35.0 41.0 30.0 41.0 52-53 36.333352602604336 39.0 35.0 40.0 29.0 41.0 54-55 36.13936145560174 39.0 35.0 40.0 28.5 41.0 56-57 35.94102833307322 38.0 35.0 40.0 28.0 41.0 58-59 35.6842409461846 38.0 34.0 40.0 28.0 41.0 60-61 35.37431615470277 38.0 34.0 40.0 27.0 41.0 62-63 35.05453057791814 37.5 34.0 40.0 26.0 41.0 64-65 34.7371261396667 37.0 33.5 40.0 26.0 41.0 66-67 34.39446431577535 36.5 33.0 39.0 26.0 41.0 68-69 33.996251950699246 36.0 32.5 39.0 25.5 40.0 70-71 33.60877241863965 35.5 32.0 38.5 25.0 40.0 72-73 33.204998277840346 35.0 32.0 38.0 24.5 40.0 74-75 32.79548305144352 35.0 31.0 37.0 23.5 39.0 76-77 31.23890218284113 33.5 29.5 35.5 21.5 38.5 78-79 32.07796709291502 34.0 31.0 36.0 23.0 39.0 80-81 32.04775458656446 34.5 31.0 36.0 23.0 37.5 82-83 31.764321470606614 34.0 31.0 35.5 22.5 37.0 84-85 31.464564263421302 34.0 31.0 35.0 21.5 37.0 86-87 31.169468257674527 34.0 30.0 35.0 20.0 36.0 88-89 30.893963012860702 34.0 30.0 35.0 20.0 36.0 90-91 30.608469845804578 34.0 30.0 35.0 19.5 35.5 92-93 30.40400959821004 34.0 29.0 35.0 19.0 35.0 94-95 30.183581766057365 34.0 29.0 35.0 18.5 35.0 96-97 29.976246070301343 34.0 29.0 35.0 18.0 35.0 98-99 29.813835799447844 34.0 29.0 35.0 18.0 35.0 100 27.91966484951907 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.31766361475531824 1101 2 0.24889600137172607 1101 3 0.31730117805319935 1101 4 0.1625606028001556 1101 5 0.17834159869556743 1101 6 0.16279976864418444 1101 7 0.23768055020364187 1101 8 0.17548430150702643 1101 9 0.2837886494318269 1101 10-11 0.24549396571838855 1101 12-13 0.2468674199390577 1101 14-15 0.2915699092926971 1101 16-17 0.33817372215909103 1101 18-19 0.31925575171316467 1101 20-21 0.27810682025159394 1101 22-23 0.2959540464667114 1101 24-25 0.2726053451774817 1101 26-27 0.23296482523985418 1101 28-29 0.31458346257843317 1101 30-31 0.34357628471507695 1101 32-33 0.4441123711553203 1101 34-35 0.3685001569807511 1101 36-37 0.3845162445043968 1101 38-39 0.40721066081965773 1101 40-41 0.3767105089996292 1101 42-43 0.38368471508995583 1101 44-45 0.3785149167262958 1101 46-47 0.45772401063366885 1101 48-49 0.40471476186683475 1101 50-51 0.411989342431589 1101 52-53 0.36191079201590526 1101 54-55 0.39358962273114884 1101 56-57 0.31899983414041344 1101 58-59 0.3231151665182779 1101 60-61 0.28844845995281077 1101 62-63 0.32524315803522796 1101 64-65 0.28849457048269045 1101 66-67 0.2713633433154641 1101 68-69 0.25267822458450695 1101 70-71 0.24002644747121593 1101 72-73 0.1969270576885478 1101 74-75 0.2174636479914298 1101 76-77 0.21248503817729514 1101 78-79 0.30800343310019684 1101 80-81 0.3014651297813984 1101 82-83 0.2769588453407863 1101 84-85 0.2630933557467472 1101 86-87 0.23679688591176884 1101 88-89 0.2247570652699622 1101 90-91 0.10701922649580453 1101 92-93 0.06087344104917136 1101 94-95 0.15290161394690216 1101 96-97 0.13907631660250885 1101 98-99 -0.014957887329423158 1101 100 -0.007321206149640602 1102 1 0.3144060157948658 1102 2 0.2532131745930357 1102 3 0.33374250404440886 1102 4 0.18759815068010965 1102 5 0.18819048449746845 1102 6 0.19988384257634806 1102 7 0.1981607337653628 1102 8 0.1982112451224154 1102 9 0.25251722439367086 1102 10-11 0.21026567653051131 1102 12-13 0.2398060156996067 1102 14-15 0.3215174191506165 1102 16-17 0.3076863333770703 1102 18-19 0.282079650233932 1102 20-21 0.28194306343883113 1102 22-23 0.22472070433375535 1102 24-25 0.2623318351427173 1102 26-27 0.24527679750311648 1102 28-29 0.3055489685072885 1102 30-31 0.31751461200187947 1102 32-33 0.32924235345784325 1102 34-35 0.3481127954946288 1102 36-37 0.3680018250933017 1102 38-39 0.3386525410585861 1102 40-41 0.34725913094117544 1102 42-43 0.25551935191994346 1102 44-45 0.2475277278578929 1102 46-47 0.2879690842364866 1102 48-49 0.37215328522277247 1102 50-51 0.37242425709025184 1102 52-53 0.2639053629512844 1102 54-55 0.3698144781461892 1102 56-57 0.3432357251721072 1102 58-59 0.39727214933468247 1102 60-61 0.37419593187839695 1102 62-63 0.3844307430145406 1102 64-65 0.3250626173649991 1102 66-67 0.27597627016629644 1102 68-69 0.2894929597566218 1102 70-71 0.2605408374078735 1102 72-73 0.29041035400796034 1102 74-75 0.3108197076169503 1102 76-77 0.3301979782275559 1102 78-79 0.3102524774233544 1102 80-81 0.30596832839943033 1102 82-83 0.2195788447769189 1102 84-85 0.18358331713522347 1102 86-87 0.19346943572037745 1102 88-89 0.19197621225389128 1102 90-91 0.16086343098693945 1102 92-93 0.11983428104522531 1102 94-95 0.18375759347882337 1102 96-97 0.17409562556774105 1102 98-99 0.11610109744254515 1102 100 0.11878414961331885 1103 1 0.28384697877316967 1103 2 0.23067125953312484 1103 3 0.2760080183016953 1103 4 0.12721049069022428 1103 5 0.17642538029719645 1103 6 0.17720152331886396 1103 7 0.1726778760218366 1103 8 0.1811440530138242 1103 9 0.23891639303950285 1103 10-11 0.19152965190315285 1103 12-13 0.23331863926311058 1103 14-15 0.3059526039936955 1103 16-17 0.33965448604941884 1103 18-19 0.3378544290217107 1103 20-21 0.3047348569907342 1103 22-23 0.32934589895472755 1103 24-25 0.24747517166422028 1103 26-27 0.2636701214054895 1103 28-29 0.3234964269603964 1103 30-31 0.4246497308611694 1103 32-33 0.42429443408087764 1103 34-35 0.4147923653406522 1103 36-37 0.42635240458503887 1103 38-39 0.40586730230445056 1103 40-41 0.4890820711332182 1103 42-43 0.45018126693823035 1103 44-45 0.3699135330943406 1103 46-47 0.45855798894194066 1103 48-49 0.39980310629116644 1103 50-51 0.46843109926617643 1103 52-53 0.46493052824900616 1103 54-55 0.5357350815076103 1103 56-57 0.4896224685180499 1103 58-59 0.5617498938545893 1103 60-61 0.4755688032766088 1103 62-63 0.5374482266382685 1103 64-65 0.5502832766929586 1103 66-67 0.5229222755969474 1103 68-69 0.5643212971679716 1103 70-71 0.5557757069285572 1103 72-73 0.5292253562403815 1103 74-75 0.4694176970626671 1103 76-77 0.5406451368134633 1103 78-79 0.5676202665603824 1103 80-81 0.4877934717372483 1103 82-83 0.5297235700543297 1103 84-85 0.4351057611218536 1103 86-87 0.4452922022366188 1103 88-89 0.42996088806427224 1103 90-91 0.5044707826639048 1103 92-93 0.4081796528649413 1103 94-95 0.4245571831876376 1103 96-97 0.49488723009272917 1103 98-99 0.5066383163609487 1103 100 0.5334934293388116 1104 1 0.25072525497454023 1104 2 0.20987906403475165 1104 3 0.27942557085303577 1104 4 0.09036123755163317 1104 5 0.15943441885865184 1104 6 0.1758263886473017 1104 7 0.2007081366371466 1104 8 0.18612137403473383 1104 9 0.23427221898759143 1104 10-11 0.251779810219098 1104 12-13 0.26360578500298715 1104 14-15 0.37449471907503096 1104 16-17 0.30970431354685246 1104 18-19 0.3349807379979097 1104 20-21 0.192705399695555 1104 22-23 0.2765108319839129 1104 24-25 0.27749917152347336 1104 26-27 0.36222585309656097 1104 28-29 0.4056643086689178 1104 30-31 0.35209967329709 1104 32-33 0.38348889308185363 1104 34-35 0.49249047479783314 1104 36-37 0.3651390935927523 1104 38-39 0.47869008777153255 1104 40-41 0.5639496331946319 1104 42-43 0.5822083476956479 1104 44-45 0.4985923551831988 1104 46-47 0.5129758269590639 1104 48-49 0.496514519421666 1104 50-51 0.5373288896975978 1104 52-53 0.5425337699616435 1104 54-55 0.5667569566106252 1104 56-57 0.6289327856156177 1104 58-59 0.5927914276374651 1104 60-61 0.5029398501854843 1104 62-63 0.5559046079643011 1104 64-65 0.6398344295835798 1104 66-67 0.5891504262967189 1104 68-69 0.6301532206140834 1104 70-71 0.5520007334965982 1104 72-73 0.6297263120552898 1104 74-75 0.6411741358873755 1104 76-77 0.5319277774984776 1104 78-79 0.6755556412809014 1104 80-81 0.7196207207261978 1104 82-83 0.5689202101917701 1104 84-85 0.6292454035966486 1104 86-87 0.7069562092107624 1104 88-89 0.6609885287322896 1104 90-91 0.5718451868779546 1104 92-93 0.5711424727213519 1104 94-95 0.5996085949890784 1104 96-97 0.5722255436031567 1104 98-99 0.6669261413452148 1104 100 0.8813550080976711 1105 1 0.24523482902849025 1105 2 0.18603961083823606 1105 3 0.22415586103996787 1105 4 0.14836474559574242 1105 5 0.10295644318114938 1105 6 0.13193123378310645 1105 7 0.1507021897676566 1105 8 0.1602407766199363 1105 9 0.1774517361017942 1105 10-11 0.16581301364140444 1105 12-13 0.20631270663136547 1105 14-15 0.28690359902986273 1105 16-17 0.29762415542912635 1105 18-19 0.33028191547806784 1105 20-21 0.21808723133947439 1105 22-23 0.3157913906730201 1105 24-25 0.30055979995940874 1105 26-27 0.3359261820213604 1105 28-29 0.37071339571281925 1105 30-31 0.4007821717616551 1105 32-33 0.41594016427087155 1105 34-35 0.4458118480903366 1105 36-37 0.44071627482532705 1105 38-39 0.46106042064602804 1105 40-41 0.49871137075218286 1105 42-43 0.48014450795017893 1105 44-45 0.4848761665866519 1105 46-47 0.5474709905101562 1105 48-49 0.5866679080615569 1105 50-51 0.5583149253114357 1105 52-53 0.5739688246667569 1105 54-55 0.5860848751129382 1105 56-57 0.6060225987702523 1105 58-59 0.6000945243530467 1105 60-61 0.5752003709688793 1105 62-63 0.5608213700582709 1105 64-65 0.6400027970278614 1105 66-67 0.6291929636655809 1105 68-69 0.5912822652464556 1105 70-71 0.6644439266854221 1105 72-73 0.610311431242323 1105 74-75 0.5877632266901855 1105 76-77 0.5476046063435156 1105 78-79 0.6732265711561496 1105 80-81 0.673394697468872 1105 82-83 0.64830146379186 1105 84-85 0.6725818896503313 1105 86-87 0.6280169440002759 1105 88-89 0.6253952808038505 1105 90-91 0.7067461786046998 1105 92-93 0.7028773348940227 1105 94-95 0.7345433722412693 1105 96-97 0.7185256327859015 1105 98-99 0.6973986069335432 1105 100 0.8926163167905337 1106 1 0.21630615654967045 1106 2 0.16681503122909902 1106 3 0.20446180340555742 1106 4 0.11991788654296442 1106 5 0.13977044935698757 1106 6 0.1509104895472575 1106 7 0.16700222091149186 1106 8 0.18979086017904478 1106 9 0.13747530770920235 1106 10-11 0.17081339021716957 1106 12-13 0.18425165657529163 1106 14-15 0.2635105903116184 1106 16-17 0.24384152310345542 1106 18-19 0.25862064639738236 1106 20-21 0.2590604252515689 1106 22-23 0.3093417877060105 1106 24-25 0.3062168228938873 1106 26-27 0.300899261942952 1106 28-29 0.35819416490444667 1106 30-31 0.3473996257430869 1106 32-33 0.395492283374665 1106 34-35 0.3889326027074631 1106 36-37 0.4337287588208554 1106 38-39 0.40131525048467864 1106 40-41 0.4620735613239404 1106 42-43 0.4077488754148533 1106 44-45 0.4094336493506816 1106 46-47 0.48683858748859166 1106 48-49 0.4895485294726427 1106 50-51 0.4892771883485878 1106 52-53 0.5146904252562479 1106 54-55 0.4844026195665876 1106 56-57 0.5059503165786268 1106 58-59 0.6083216667129392 1106 60-61 0.6032200913257526 1106 62-63 0.5846841621334846 1106 64-65 0.6306227425159747 1106 66-67 0.6391346249550267 1106 68-69 0.6359816865411076 1106 70-71 0.6239245167491134 1106 72-73 0.6375388972538119 1106 74-75 0.68989676249236 1106 76-77 0.5943695558925448 1106 78-79 0.6525649377129668 1106 80-81 0.6234385023904068 1106 82-83 0.6453814953020895 1106 84-85 0.5732833587029234 1106 86-87 0.49980270699366613 1106 88-89 0.5801624450254863 1106 90-91 0.6074857530537301 1106 92-93 0.6715505668708026 1106 94-95 0.7333840481235256 1106 96-97 0.769366801269971 1106 98-99 0.7929801315157974 1106 100 0.9988302647497989 1107 1 0.17142058265819315 1107 2 0.16911011713744273 1107 3 0.20241645347096693 1107 4 0.10943956684993594 1107 5 0.09731343368163436 1107 6 0.05902202616537977 1107 7 0.10307727173915282 1107 8 0.09826739679902374 1107 9 0.17194670395998912 1107 10-11 0.17069583776189745 1107 12-13 0.13942770911856428 1107 14-15 0.17533410862628784 1107 16-17 0.15675943420566085 1107 18-19 0.134140205076271 1107 20-21 0.21119614947081544 1107 22-23 0.27726645317947174 1107 24-25 0.28385167724232474 1107 26-27 0.2766097844986888 1107 28-29 0.2524592549026252 1107 30-31 0.17277341698160598 1107 32-33 0.2169320122133982 1107 34-35 0.23940779626028075 1107 36-37 0.29091680148324883 1107 38-39 0.3265508397238932 1107 40-41 0.28096902179906635 1107 42-43 0.31840499020169233 1107 44-45 0.33127213042656223 1107 46-47 0.3031344566668892 1107 48-49 0.37855746578071603 1107 50-51 0.2905457700983405 1107 52-53 0.3060152126772664 1107 54-55 0.33803188123035 1107 56-57 0.3939113721747276 1107 58-59 0.4545190752798405 1107 60-61 0.4270003813339187 1107 62-63 0.405795390144398 1107 64-65 0.4721580301247954 1107 66-67 0.4772954036514321 1107 68-69 0.47838392867098634 1107 70-71 0.51460807779128 1107 72-73 0.43677263765868446 1107 74-75 0.48254227806116745 1107 76-77 0.47464657289220824 1107 78-79 0.5737320256526317 1107 80-81 0.5954598159913829 1107 82-83 0.5804340570455295 1107 84-85 0.457953453631756 1107 86-87 0.4174133767150927 1107 88-89 0.4287268349425766 1107 90-91 0.37102700651781717 1107 92-93 0.39039558694142684 1107 94-95 0.5565175600450516 1107 96-97 0.609247324718357 1107 98-99 0.7586232722837671 1107 100 0.9247888317433173 1108 1 0.2005152415517557 1108 2 0.1361145405404045 1108 3 0.15737726437327382 1108 4 0.12039612753227402 1108 5 0.12835851399545106 1108 6 0.13760654387237992 1108 7 0.1095547979607403 1108 8 0.128694142308035 1108 9 0.16338749658908114 1108 10-11 0.1926321084184579 1108 12-13 0.20400177296136945 1108 14-15 0.23306004962462623 1108 16-17 0.25830545005697303 1108 18-19 0.24699546103024517 1108 20-21 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-0.26203053437129853 2316 62-63 -0.30937982045153234 2316 64-65 -0.3920169113254701 2316 66-67 -0.16393250532117776 2316 68-69 -0.14309254598236265 2316 70-71 -0.38129453763050947 2316 72-73 -0.41369357242400895 2316 74-75 -0.41902117330193533 2316 76-77 -0.27440883887069845 2316 78-79 -0.2820892168314586 2316 80-81 -0.2417241182160197 2316 82-83 -0.20102202306538786 2316 84-85 -0.21524809521375587 2316 86-87 -0.17735481865413405 2316 88-89 -0.09562283866296184 2316 90-91 -0.0903654105128382 2316 92-93 0.004497759572505089 2316 94-95 0.04620510625807128 2316 96-97 0.008435202297764732 2316 98-99 -0.11225973540519263 2316 100 -0.23692119487798635 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 6.0 10 11.0 11 40.0 12 120.0 13 262.0 14 889.0 15 2355.0 16 5163.0 17 10946.0 18 19057.0 19 29905.0 20 42266.0 21 59299.0 22 77036.0 23 89561.0 24 96766.0 25 107304.0 26 124963.0 27 149818.0 28 180825.0 29 218723.0 30 264282.0 31 319126.0 32 390261.0 33 492526.0 34 646438.0 35 881870.0 36 1302263.0 37 2038315.0 38 2052165.0 39 335493.0 40 48.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.28101394318798 20.178911617471794 13.286470305771243 24.253604133568988 2 15.072137868965156 21.530143883030327 39.511812090822595 23.88590615718192 3 17.161097021584546 26.953575132953834 30.95807863847545 24.92724920698617 4 11.874758881172417 17.38634536406855 38.46828642169698 32.27060933306205 5 12.306046401549455 38.65808662234362 35.84277593031315 13.193091045793778 6 30.745869857156862 38.39739328724374 17.810826567910716 13.045910287688692 7 26.66413298393063 33.05662056430689 23.043341370078373 17.2359050816841 8 25.71202110522496 36.43486882136298 20.47274440071672 17.380365672695337 9 26.47853951172362 14.823848137586848 20.420209052011042 38.27740329867849 10-11 24.34646732882523 26.53257367125296 29.39417110086301 19.726787899058802 12-13 24.859462615752726 24.32851118568604 29.334396111612044 21.47763008694919 14-15 22.218541103870628 25.75829612552818 26.543853489946724 25.479309280654466 16-17 21.852248849180583 28.68214039456019 27.32547278622443 22.140137970034797 18-19 21.766860862493104 27.863182629691057 29.142409797552173 21.227546710263667 20-21 22.441503774015796 27.110137987381762 29.137406415623087 21.310951822979362 22-23 22.2053214990146 26.997242531823478 28.931968096121373 21.865467873040547 24-25 22.012186029082816 27.15804788479732 29.065962494649384 21.763803591470484 26-27 22.01717784373611 27.289864730077728 28.994890115985537 21.698067310200624 28-29 22.020310785231327 27.161017629214996 29.166487430771546 21.65218415478213 30-31 22.20050580846566 27.158112011313396 29.103150684524877 21.53823149569607 32-33 22.020777395925606 27.351766016265742 28.975688730092592 21.651767857716063 34-35 22.19169981027278 27.363853274330342 29.00528628966384 21.439160625733038 36-37 22.284483996905404 27.237649223782505 28.877742971312497 21.600123807999594 38-39 22.11945680558731 27.41131673809654 28.88697288938405 21.5822535669321 40-41 22.135366627698378 27.278813452084727 29.007043222599492 21.5787766976174 42-43 22.001429745056974 27.327381026655168 28.85354934612912 21.817639882158737 44-45 22.03149704946291 27.49801448488933 28.71466166747043 21.755826798177328 46-47 22.106597986135423 27.460084925540468 28.488773383731434 21.944543704592675 48-49 22.047279038141106 27.539357436427636 28.49448509840441 21.918878427026854 50-51 22.090573060278473 27.684246896569835 28.293443355496866 21.931736687654823 52-53 22.1296657713908 27.731710868459853 28.196067930679064 21.942555429470286 54-55 22.032724401983543 27.72254268254243 28.277450072447586 21.967282843026446 56-57 22.098373826093667 27.775165435399767 28.100194941228334 22.02626579727823 58-59 22.061747823950622 27.675495103715953 28.259457848355947 22.00329922397748 60-61 22.188872189833212 27.830745706478233 28.158227214384514 21.822154889304045 62-63 22.008041557624967 27.793827895450598 28.300602918064865 21.897527628859578 64-65 21.93880313126843 27.611485074309964 28.265555061141928 22.18415673327968 66-67 21.926422144454012 27.713122351491705 28.132631926714485 22.2278235773398 68-69 21.86500676193437 27.748762676960176 28.209974050696317 22.176256510409132 70-71 21.90164798181121 27.55061132277184 28.190341501712346 22.357399193704605 72-73 21.871569288010924 27.791170292322885 27.94389208672875 22.393368332937435 74-75 21.81339888773197 27.865576922060985 27.912357887973727 22.408666302233314 76-77 21.751994140756384 27.73628553558853 28.04501579147058 22.466704532184508 78-79 21.727314228199653 27.681303052077332 28.0332330164798 22.558149703243217 80-81 21.74302323486914 27.90253223112686 27.796610442382963 22.55783409162104 82-83 21.83871260730935 27.810633856378757 27.861119350919473 22.48953418539242 84-85 21.71792220046822 27.828164001337313 27.79552125637985 22.658392541814617 86-87 21.61415970459317 27.809156271069526 27.959344624223153 22.617339400114155 88-89 21.57515649798578 27.715726982629462 27.94178899426283 22.76732752512193 90-91 21.724851658590566 27.780120062059073 27.808180504735702 22.68684777461466 92-93 21.654639160729914 27.691492912538724 27.814687025150565 22.8391809015808 94-95 21.47588465106316 27.559256281741895 27.903109486083434 23.061749581111513 96-97 21.531624125689724 27.511878220163144 27.893230280061097 23.063267374086035 98-99 21.420024115643173 27.498596306931088 27.821845761531293 23.259533815894446 100 20.6960917427444 27.87989073107185 27.979818077681255 23.44419944850249 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2987.0 1 2263.5 2 2312.0 3 3259.5 4 2739.0 5 2498.0 6 3928.0 7 7475.0 8 11621.0 9 13456.0 10 11771.5 11 9518.0 12 9426.0 13 10015.5 14 10688.0 15 11459.0 16 12312.0 17 13080.5 18 14112.5 19 15403.0 20 17195.0 21 20176.5 22 24214.5 23 30139.0 24 38038.0 25 47495.5 26 59710.5 27 73605.0 28 87702.0 29 104795.5 30 124950.5 31 142548.5 32 162087.0 33 186571.0 34 211659.5 35 234863.5 36 264199.0 37 292384.5 38 309893.0 39 323525.5 40 328465.0 41 330279.5 42 334931.0 43 342318.5 44 350989.5 45 361859.5 46 369115.0 47 370419.5 48 374986.5 49 375847.0 50 370445.0 51 357357.0 52 340164.0 53 327182.5 54 311540.0 55 288432.0 56 260904.5 57 232844.5 58 204921.5 59 174613.5 60 143439.0 61 115414.5 62 91010.0 63 69269.0 64 50635.0 65 36472.5 66 26278.0 67 19532.5 68 14648.5 69 10736.0 70 7925.0 71 5838.5 72 4271.5 73 3095.5 74 2318.5 75 1650.5 76 1081.0 77 691.0 78 453.5 79 314.5 80 209.0 81 147.5 82 113.5 83 82.0 84 64.5 85 56.5 86 34.0 87 20.5 88 18.5 89 12.0 90 11.0 91 9.5 92 5.0 93 4.0 94 7.5 95 7.0 96 4.0 97 5.0 98 5.5 99 4.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0034916120309882075 2 0.07093909169586993 3 6.741729281735156E-4 4 5.735501030729908E-4 5 0.0015999029190983428 6 6.942974931936206E-4 7 0.0 8 9.056054259047225E-4 9 2.0124565020104945E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.597023295089616E-4 18-19 0.001111882217360798 20-21 0.003486580889733181 22-23 1.0062282510052472E-5 24-25 1.0062282510052472E-5 26-27 1.0565396635555096E-4 28-29 8.301383070793289E-4 30-31 0.0015244358002729495 32-33 0.002857688232854902 34-35 0.0041708161004167495 36-37 0.0013533769976020575 38-39 0.0015194046590179232 40-41 0.005388352284133099 42-43 0.006711542434204999 44-45 0.0050864838088315245 46-47 0.00696309949695631 48-49 0.008502628720994339 50-51 0.008376850189618684 52-53 0.003863916483860149 54-55 0.0022992315535469896 56-57 0.002374698672372383 58-59 0.003134401001881345 60-61 0.007526587317519249 62-63 0.013548863399785654 64-65 0.004075224416571251 66-67 0.010298746149038706 68-69 0.01235145178108941 70-71 0.00557450451056907 72-73 0.0075718675888144845 74-75 0.003023715894270768 76-77 0.00303377817678082 78-79 0.007612116718854696 80-81 0.005856248420850538 82-83 0.015953748919688194 84-85 0.017558682980041564 86-87 0.02259988651757785 88-89 0.021256571802485847 90-91 0.024229976284206353 92-93 0.03396020347142709 94-95 0.03800020989921316 96-97 0.022851443580329162 98-99 0.018252980473235186 100 0.018957340248938855 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9938103.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.99646402773578 #Duplication Level Percentage of deduplicated Percentage of total 1 70.73396358254244 38.901178837064684 2 16.678732982159055 18.345426769630418 3 5.806618481135726 9.580304532617003 4 2.6045573587844024 5.729657803622436 5 1.3215913348219221 3.6341425152450575 6 0.7812603965146473 2.5779935575927437 7 0.47128064419225296 1.8143138296701171 8 0.33335168635972134 1.466653122197398 9 0.22862044200131268 1.1315984323077222 >10 0.9638682741397487 8.81501382759639 >50 0.0439429310364674 1.6585524333414563 >100 0.028567236462006384 3.0601112193028697 >500 0.0023348921815729133 0.8421647640335672 >1k 0.0011958656977228857 1.0679532958836797 >5k 2.8472992754717352E-5 0.08665767643223461 >10k+ 8.541897826415206E-5 1.2882773834622228 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32730 0.3293385065540174 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28965 0.29145401290366985 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20575 0.2070314626443296 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.0124565020104945E-5 2 0.0 0.0 0.0 0.0 2.0124565020104945E-5 3 0.0 0.0 0.0 0.0 2.0124565020104945E-5 4 0.0 0.0 0.0 1.0062282510052472E-5 3.0186847530157416E-5 5 1.0062282510052472E-5 3.0186847530157416E-5 0.0 1.0062282510052472E-5 3.0186847530157416E-5 6 1.0062282510052472E-5 3.0186847530157416E-5 0.0 1.0062282510052472E-5 4.024913004020989E-5 7 2.0124565020104945E-5 3.0186847530157416E-5 0.0 1.0062282510052472E-5 4.024913004020989E-5 8 2.0124565020104945E-5 3.0186847530157416E-5 0.0 1.0062282510052472E-5 6.037369506031483E-5 9 2.0124565020104945E-5 3.0186847530157416E-5 0.0 4.024913004020989E-5 8.049826008041978E-5 10-11 2.515570627513118E-5 3.0186847530157416E-5 0.0 4.024913004020989E-5 8.049826008041978E-5 12-13 3.0186847530157416E-5 5.031141255026236E-5 0.0 4.024913004020989E-5 8.049826008041978E-5 14-15 3.521798878518365E-5 5.031141255026236E-5 0.0 4.528027129523613E-5 1.1571624886560342E-4 16-17 4.528027129523613E-5 5.031141255026236E-5 0.0 5.031141255026236E-5 1.5596537890581332E-4 18-19 5.031141255026236E-5 5.031141255026236E-5 0.0 6.037369506031483E-5 1.7105880267089202E-4 20-21 5.031141255026236E-5 1.0062282510052471E-4 0.0 7.546711882539354E-5 1.7105880267089202E-4 22-23 8.049826008041978E-5 1.0062282510052471E-4 0.0 1.4590309639576086E-4 2.1633907396612816E-4 24-25 1.1068510761057719E-4 1.0062282510052471E-4 0.0 3.1193075781162663E-4 2.6161934526136425E-4 26-27 1.1068510761057719E-4 1.0062282510052471E-4 0.0 4.779584192274924E-4 2.666504865163905E-4 28-29 1.3080967263068212E-4 1.1571624886560342E-4 0.0 0.0018413976993396023 2.7168162777141674E-4 30-31 1.6602766141586578E-4 1.408719551407346E-4 0.0 0.006515327925258976 2.867750515364954E-4 32-33 1.7105880267089202E-4 1.408719551407346E-4 5.031141255026236E-6 0.016331084513815163 3.1193075781162663E-4 34-35 1.7105880267089202E-4 1.4590309639576086E-4 1.0062282510052472E-5 0.03118301349865261 3.219930403216791E-4 36-37 1.8112108518094448E-4 1.6602766141586578E-4 1.0062282510052472E-5 0.05575510738820075 3.219930403216791E-4 38-39 1.8112108518094448E-4 1.9118336769099694E-4 1.0062282510052472E-5 0.09308617550049542 3.219930403216791E-4 40-41 1.8112108518094448E-4 2.0124565020104943E-4 1.0062282510052472E-5 0.13533769976020577 3.3205532283173155E-4 42-43 1.8112108518094448E-4 2.0124565020104943E-4 1.0062282510052472E-5 0.17715151473072877 3.370864640867578E-4 44-45 1.8112108518094448E-4 2.0627679145607567E-4 1.0062282510052472E-5 0.22926407585029054 3.5217988785183653E-4 46-47 1.8112108518094448E-4 2.1130793271110192E-4 1.0062282510052472E-5 0.2948500332508126 3.7230445287194145E-4 48-49 1.9621450894602319E-4 2.1633907396612816E-4 1.0062282510052472E-5 0.36607086885696394 4.2261586542220383E-4 50-51 2.1633907396612816E-4 2.3143249773120684E-4 1.0062282510052472E-5 0.44221719175178603 4.2261586542220383E-4 52-53 2.2137021522115438E-4 2.3143249773120684E-4 1.0062282510052472E-5 0.5192842134962778 4.678961367174399E-4 54-55 2.3646363898623308E-4 2.3646363898623308E-4 1.0062282510052472E-5 0.6040840993497452 5.232386905227285E-4 56-57 2.515570627513118E-4 2.6161934526136425E-4 1.0062282510052472E-5 0.707438834151749 5.383321142878072E-4 58-59 2.918061927915217E-4 2.76712769026443E-4 1.0062282510052472E-5 0.8261938923353884 5.483943967978598E-4 60-61 3.0186847530157414E-4 3.1193075781162663E-4 1.0062282510052472E-5 0.9562086446477763 5.836123855830433E-4 62-63 3.1193075781162663E-4 3.1193075781162663E-4 1.0062282510052472E-5 1.0869981927134384 5.936746680930958E-4 64-65 3.219930403216791E-4 3.1193075781162663E-4 1.0062282510052472E-5 1.221923338890732 5.936746680930958E-4 66-67 3.219930403216791E-4 3.3205532283173155E-4 1.0062282510052472E-5 1.3790660048502215 5.987058093481221E-4 68-69 3.219930403216791E-4 3.3205532283173155E-4 1.0062282510052472E-5 1.5617568061027343 6.188303743682271E-4 70-71 3.219930403216791E-4 3.4714874659681023E-4 1.5093423765078708E-5 1.7670223381665495 6.339237981333058E-4 72-73 3.219930403216791E-4 3.6224217036188896E-4 2.0124565020104945E-5 1.9729318563110083 7.546711882539354E-4 74-75 3.219930403216791E-4 3.6224217036188896E-4 2.0124565020104945E-5 2.175712004594841 7.647334707639879E-4 76-77 3.219930403216791E-4 3.6224217036188896E-4 2.0124565020104945E-5 2.4023548558512626 7.697646120190141E-4 78-79 3.219930403216791E-4 3.6224217036188896E-4 2.0124565020104945E-5 2.665916221637067 7.747957532740404E-4 80-81 3.3205532283173155E-4 3.7230445287194145E-4 2.0124565020104945E-5 2.9531038267564744 7.999514595491715E-4 82-83 3.4211760534178404E-4 3.7230445287194145E-4 2.0124565020104945E-5 3.252461762571791 8.452317308444077E-4 84-85 3.4211760534178404E-4 3.7230445287194145E-4 2.0124565020104945E-5 3.5521265980036634 8.452317308444077E-4 86-87 3.4211760534178404E-4 3.9746015914707267E-4 2.0124565020104945E-5 3.862482608602467 8.703874371195387E-4 88 3.4211760534178404E-4 4.0249130040209886E-4 2.0124565020104945E-5 4.114326446405315 8.9554314339467E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15875 0.0 47.897987 1 GTATCAA 27075 0.0 36.507767 1 TCAACGC 33360 0.0 29.060966 4 GTACATG 33535 0.0 29.012371 1 ATCAACG 33625 0.0 28.734396 3 CAACGCA 33770 0.0 28.721909 5 AACGCAG 35015 0.0 27.821175 6 TACATGG 34535 0.0 27.449924 2 TATCAAC 35610 0.0 27.294716 2 ACATGGG 33965 0.0 26.827946 3 TACCTGG 7970 0.0 26.313984 2 CATGGGG 18575 0.0 24.616192 4 GTACCTG 8975 0.0 24.206306 1 ACGCAGA 40180 0.0 24.081001 7 CGCAGAG 41060 0.0 23.370333 8 ATGGGGG 10865 0.0 21.279922 5 GCAGAGT 46030 0.0 20.438398 9 ACCTGGG 10380 0.0 19.693964 3 GAGTACT 28130 0.0 19.33725 12-13 AGAGTAC 42400 0.0 19.318083 10-11 >>END_MODULE