FastQCFastQC Report
Mon 6 Jun 2016
ERR1211524_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211524_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6119544
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT187930.3070980452138264No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169740.2773736082296328No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT109190.17842832733942268No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA94400.049.1780781
GTATCAA157750.036.263821
TACCTGG56700.036.1547852
GTACCTG63750.032.865971
TCAACGC187200.029.5443044
ATCAACG189700.029.204733
CAACGCA189600.029.1190935
AACGCAG197300.028.1907796
TATCAAC202700.027.672542
ACCTGGG74500.027.1218243
GTACATG176950.026.1825031
TACATGG181200.025.0917342
ACGCAGA224550.024.6661247
ACATGGG179200.024.2555083
CGCAGAG230950.023.9023938
CATGGGG113400.021.2987044
GCAGAGT259200.020.5914139
GAGTACT164150.019.4217812-13
AGAGTAC235800.019.23777410-11
CAGAGTA250900.018.87583510-11
Produced by FastQC (version 0.11.5)