##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211524_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6119544 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.311369605316997 31.0 31.0 34.0 28.0 34.0 2 31.343053175203902 31.0 31.0 34.0 28.0 34.0 3 31.44662984692977 33.0 31.0 34.0 28.0 34.0 4 34.983969720619704 37.0 35.0 37.0 32.0 37.0 5 35.072822746269985 37.0 35.0 37.0 32.0 37.0 6 35.18863072150474 37.0 35.0 37.0 32.0 37.0 7 35.11468305481585 37.0 35.0 37.0 32.0 37.0 8 35.081940909322654 37.0 35.0 37.0 32.0 37.0 9 36.652665460040815 39.0 37.0 39.0 32.0 39.0 10-11 36.63917335997584 39.0 37.0 39.0 32.0 39.0 12-13 36.58452623267355 39.0 37.0 39.0 32.0 39.0 14-15 37.777352201405854 40.0 38.0 41.0 32.0 41.0 16-17 37.760650875293976 40.0 38.0 41.0 32.0 41.0 18-19 37.76870041297195 40.0 38.0 41.0 32.0 41.0 20-21 37.72110528170073 40.0 37.5 41.0 32.0 41.0 22-23 37.57555955149599 40.0 37.0 41.0 32.0 41.0 24-25 37.491733452688635 40.0 37.0 41.0 31.0 41.0 26-27 37.349972645020614 40.0 37.0 41.0 31.0 41.0 28-29 37.19184999732006 40.0 36.5 41.0 30.5 41.0 30-31 37.02235648603883 40.0 36.0 41.0 30.0 41.0 32-33 36.867708361930234 39.0 36.0 41.0 30.0 41.0 34-35 36.70256149804626 39.0 36.0 41.0 30.0 41.0 36-37 36.534160225010226 39.0 35.0 41.0 30.0 41.0 38-39 36.408702674578365 39.0 35.0 40.5 29.0 41.0 40-41 36.381958933541455 39.0 35.0 41.0 29.0 41.0 42-43 36.32618941868871 39.0 35.0 41.0 29.0 41.0 44-45 36.1499987580774 39.0 35.0 40.0 28.0 41.0 46-47 36.10883645905643 39.0 35.0 40.5 28.0 41.0 48-49 36.07922387681174 39.0 35.0 41.0 27.0 41.0 50-51 35.11010689685375 38.0 34.0 40.0 25.5 40.5 52-53 35.32142811621258 38.5 34.5 39.5 26.5 40.5 54-55 35.90878969086586 39.0 35.0 40.5 27.0 41.0 56-57 35.87514412838604 39.0 35.0 41.0 27.0 41.0 58-59 35.71413923978649 39.0 35.0 41.0 26.5 41.0 60-61 35.526820544145124 38.0 34.0 40.0 26.0 41.0 62-63 35.278845858449586 38.0 34.0 40.0 26.0 41.0 64-65 35.00003292729001 37.5 34.0 40.0 26.0 41.0 66-67 34.69234268108866 37.0 34.0 40.0 26.0 41.0 68-69 34.408071336687826 36.5 33.5 39.5 26.0 41.0 70-71 34.06712223982702 36.0 33.0 39.0 25.0 41.0 72-73 33.71801362977372 35.5 33.0 39.0 25.0 40.5 74-75 33.351945259319976 35.0 32.5 37.5 24.5 40.0 76-77 33.01142805738467 35.0 32.0 37.0 24.0 39.0 78-79 32.684876356800444 35.0 32.0 37.0 24.0 39.0 80-81 32.346851497431835 35.0 32.0 36.0 23.0 39.0 82-83 32.04849193011767 35.0 31.5 36.0 23.0 37.0 84-85 31.783710926827226 35.0 31.0 35.5 22.0 37.0 86-87 31.490631900023924 35.0 31.0 35.0 20.5 36.5 88-89 31.26323644376117 34.0 31.0 35.0 20.0 36.0 90-91 31.031535846461765 34.0 31.0 35.0 20.0 36.0 92-93 30.885665337155842 34.0 30.5 35.0 20.0 36.0 94-95 30.728819336865623 34.0 30.0 35.0 19.0 35.0 96-97 30.627703469408832 34.0 30.0 35.0 19.0 35.0 98-99 30.455143471474344 34.0 30.0 35.0 18.5 35.0 100 29.05921192820903 33.0 27.0 35.0 15.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33134857409370255 1101 2 0.33467111779205183 1101 3 0.3297573761754684 1101 4 0.3050618087014101 1101 5 0.2672529456397754 1101 6 0.3254906850099246 1101 7 0.37541468970771774 1101 8 0.37885444665361945 1101 9 0.36522006204442903 1101 10-11 0.3457084358478042 1101 12-13 0.3237359206470174 1101 14-15 0.44506270353601707 1101 16-17 0.4200106016193601 1101 18-19 0.37899560076267846 1101 20-21 0.3905344640573958 1101 22-23 0.37167364762746047 1101 24-25 0.32661234259976624 1101 26-27 0.3245863276161245 1101 28-29 0.3683666704300137 1101 30-31 0.415661725041538 1101 32-33 0.32210555423831266 1101 34-35 0.28233318226817516 1101 36-37 0.25791801681666016 1101 38-39 0.30584209557951425 1101 40-41 0.235748162237293 1101 42-43 0.10270755095065454 1101 44-45 0.10408172926263148 1101 46-47 0.08622149907426291 1101 48-49 0.14646607829855185 1101 50-51 0.23832708059077845 1101 52-53 0.3450091586032684 1101 54-55 0.3401532274564403 1101 56-57 0.30036024249318416 1101 58-59 0.25636958699325874 1101 60-61 0.15377573797913158 1101 62-63 0.23206990413203243 1101 64-65 0.24843351415119486 1101 66-67 0.2571967789386207 1101 68-69 0.2267314169233643 1101 70-71 0.18273463193414585 1101 72-73 0.18816038394730583 1101 74-75 0.09158737620185775 1101 76-77 0.1383729025579541 1101 78-79 0.008784384361867126 1101 80-81 0.026738470501136646 1101 82-83 0.02679597416192081 1101 84-85 -0.04560922242805532 1101 86-87 -0.037922507619168044 1101 88-89 -0.1514181993730972 1101 90-91 -0.1636459179693155 1101 92-93 -0.24998143590757493 1101 94-95 -0.3333096321733855 1101 96-97 -0.35380624081361844 1101 98-99 -0.3919756752600634 1101 100 -0.3255708907092405 1102 1 0.42199210186120695 1102 2 0.39430079871756973 1102 3 0.37984722625257916 1102 4 0.2987207146384421 1102 5 0.39227656231661 1102 6 0.3849954991535398 1102 7 0.3818470057709362 1102 8 0.41201707355106976 1102 9 0.4160934326679353 1102 10-11 0.40708640415595454 1102 12-13 0.4176458014116591 1102 14-15 0.5405645377754738 1102 16-17 0.5234445252280651 1102 18-19 0.49204764194475814 1102 20-21 0.4882191804416891 1102 22-23 0.5023388844453436 1102 24-25 0.557190331639184 1102 26-27 0.44520794381126194 1102 28-29 0.42362442430003 1102 30-31 0.41462366920034555 1102 32-33 0.34788331488519475 1102 34-35 0.49342653096704936 1102 36-37 0.417071803738267 1102 38-39 0.4183919040111306 1102 40-41 0.4344611350118015 1102 42-43 0.4143668179430122 1102 44-45 0.3075168396937329 1102 46-47 0.32843836135975124 1102 48-49 0.4568622287257824 1102 50-51 0.47182961777751586 1102 52-53 0.5030364659711068 1102 54-55 0.5597470881221795 1102 56-57 0.6032173050881582 1102 58-59 0.5292786563633527 1102 60-61 0.5949045454469299 1102 62-63 0.5230000222872775 1102 64-65 0.5591043758539413 1102 66-67 0.4802004783402367 1102 68-69 0.4787540562682935 1102 70-71 0.475191602544939 1102 72-73 0.40258827893377713 1102 74-75 0.38148576460592665 1102 76-77 0.3510694741002567 1102 78-79 0.2935553842586529 1102 80-81 0.30298932686427094 1102 82-83 0.28565555978887147 1102 84-85 0.17760819211093448 1102 86-87 0.21936629134705754 1102 88-89 0.19111608890584364 1102 90-91 0.10892966519601188 1102 92-93 0.17283423380650476 1102 94-95 0.05021406093916525 1102 96-97 0.013114079197251272 1102 98-99 -0.03626439191090114 1102 100 -0.01700978547053822 1103 1 0.365516074522656 1103 2 0.357577376730422 1103 3 0.28948725721482305 1103 4 0.3161851733332526 1103 5 0.29015931458042843 1103 6 0.28503591622074964 1103 7 0.2675344849195511 1103 8 0.2806523085304917 1103 9 0.27979251078957645 1103 10-11 0.32406289104908836 1103 12-13 0.3065582064842971 1103 14-15 0.45478765317312053 1103 16-17 0.39241232739674103 1103 18-19 0.30805543788934386 1103 20-21 0.3471249790051303 1103 22-23 0.3978047554298456 1103 24-25 0.3829108828936967 1103 26-27 0.38717009022785476 1103 28-29 0.4124914673135507 1103 30-31 0.4315920133401363 1103 32-33 0.31836032858480223 1103 34-35 0.3713086370852565 1103 36-37 0.40672730922852907 1103 38-39 0.3867553622351849 1103 40-41 0.3261118458379073 1103 42-43 0.2883922589373853 1103 44-45 0.2453639946091215 1103 46-47 0.30723858764257983 1103 48-49 0.3669117050181754 1103 50-51 0.35765938082669635 1103 52-53 0.35797595245677627 1103 54-55 0.4020883176185919 1103 56-57 0.3928204925640131 1103 58-59 0.4664924896815208 1103 60-61 0.3621882482041201 1103 62-63 0.31498527829940315 1103 64-65 0.32325516250325137 1103 66-67 0.4229516146993788 1103 68-69 0.40695287263503843 1103 70-71 0.4528942384234256 1103 72-73 0.39531686612981076 1103 74-75 0.43348476110223544 1103 76-77 0.2779384173093362 1103 78-79 0.20489444850794314 1103 80-81 0.22995898307524953 1103 82-83 0.237692467889822 1103 84-85 0.14684299087613795 1103 86-87 0.1789300046008222 1103 88-89 0.22095097232930883 1103 90-91 0.11315183115521776 1103 92-93 -0.07233694862677709 1103 94-95 -0.052204402737562106 1103 96-97 -0.020731027832152193 1103 98-99 0.06979721213898316 1103 100 0.0178791763844508 1104 1 0.47068616861559676 1104 2 0.45150084687842806 1104 3 0.42990312724761637 1104 4 0.37681612473674164 1104 5 0.3267163053440356 1104 6 0.32720892055243667 1104 7 0.3475547553688756 1104 8 0.38231773490284127 1104 9 0.4637658012361001 1104 10-11 0.5292758741285155 1104 12-13 0.5023527941961277 1104 14-15 0.5106439601891921 1104 16-17 0.5575269318949054 1104 18-19 0.46594639234252355 1104 20-21 0.4063259427420576 1104 22-23 0.5190642471568054 1104 24-25 0.5243368868478697 1104 26-27 0.5951062470010626 1104 28-29 0.5345648940403933 1104 30-31 0.5444141694712457 1104 32-33 0.49481005018107993 1104 34-35 0.4551678899632421 1104 36-37 0.4550640550003493 1104 38-39 0.5134874517118817 1104 40-41 0.4029458740401566 1104 42-43 0.21040924249816584 1104 44-45 0.279992479760125 1104 46-47 0.3423030981520654 1104 48-49 0.43133224429206507 1104 50-51 0.486979862451868 1104 52-53 0.6798693735968584 1104 54-55 0.6438628203822105 1104 56-57 0.6173378964346838 1104 58-59 0.5854542112882299 1104 60-61 0.6428061080947387 1104 62-63 0.6559149581731418 1104 64-65 0.5334879598817324 1104 66-67 0.53857874747343 1104 68-69 0.47246390430358787 1104 70-71 0.3968156124523219 1104 72-73 0.5454789623879392 1104 74-75 0.5322363429662147 1104 76-77 0.5068277065967948 1104 78-79 0.41241874502647136 1104 80-81 0.40555625755563796 1104 82-83 0.45108616945372404 1104 84-85 0.3669693727399519 1104 86-87 0.34672703336826416 1104 88-89 0.4104367628138412 1104 90-91 0.37559676187033375 1104 92-93 0.40525702593220103 1104 94-95 0.5149886939276342 1104 96-97 0.4840034210275377 1104 98-99 0.41342535756664134 1104 100 0.4787159384473476 1105 1 0.40517321018547037 1105 2 0.419726318885413 1105 3 0.38762647632750813 1105 4 0.34081211274233425 1105 5 0.2984034217490432 1105 6 0.2948661999144022 1105 7 0.35400192298051536 1105 8 0.33911785754930435 1105 9 0.4128225667994698 1105 10-11 0.4147317014811023 1105 12-13 0.4385379609035027 1105 14-15 0.48335317374473874 1105 16-17 0.45085343998731986 1105 18-19 0.45191584505867155 1105 20-21 0.48561558067515165 1105 22-23 0.6121811909039963 1105 24-25 0.5115166505282289 1105 26-27 0.5358821244516037 1105 28-29 0.5494516712621049 1105 30-31 0.5742021452676269 1105 32-33 0.5462121261629918 1105 34-35 0.5305139591268286 1105 36-37 0.5690955550462462 1105 38-39 0.6521391894632629 1105 40-41 0.6269770016427145 1105 42-43 0.49141515135521985 1105 44-45 0.5688790300512423 1105 46-47 0.4494770932912431 1105 48-49 0.49886778664753706 1105 50-51 0.5885618805404889 1105 52-53 0.602406007297958 1105 54-55 0.6130566017908876 1105 56-57 0.6077151728863868 1105 58-59 0.6505474347247926 1105 60-61 0.6393248021524514 1105 62-63 0.6527418707563584 1105 64-65 0.603925702078179 1105 66-67 0.5830696174144805 1105 68-69 0.5377989560732175 1105 70-71 0.5883519672539776 1105 72-73 0.5365249911890757 1105 74-75 0.49830255300001625 1105 76-77 0.5313278096683263 1105 78-79 0.47125271151350034 1105 80-81 0.4534697952651001 1105 82-83 0.43901081983335644 1105 84-85 0.37429407751363897 1105 86-87 0.49781002067573965 1105 88-89 0.5083585289228836 1105 90-91 0.44498109569044075 1105 92-93 0.4511835783713636 1105 94-95 0.4459341457014183 1105 96-97 0.4353986111382113 1105 98-99 0.49774882721424163 1105 100 0.5496558295553058 1106 1 0.34337960716718285 1106 2 0.3371754810308154 1106 3 0.24786174447020315 1106 4 0.23321330239521387 1106 5 0.2708715943136468 1106 6 0.2855720368111747 1106 7 0.2586210814569512 1106 8 0.27828451609879323 1106 9 0.30618482973742545 1106 10-11 0.3044562533319777 1106 12-13 0.31891842903710454 1106 14-15 0.40120697696498553 1106 16-17 0.3734395550758478 1106 18-19 0.421818585091593 1106 20-21 0.3300361606309252 1106 22-23 0.36471496646091595 1106 24-25 0.41436991801505485 1106 26-27 0.44386828629505004 1106 28-29 0.43968029955727417 1106 30-31 0.5082225161250307 1106 32-33 0.5085691421603187 1106 34-35 0.5342872270032828 1106 36-37 0.4849521278459292 1106 38-39 0.4955544575410116 1106 40-41 0.5113535540520502 1106 42-43 0.4934890890607804 1106 44-45 0.5080270191027907 1106 46-47 0.5551886609121723 1106 48-49 0.6015382553980402 1106 50-51 0.548084379720656 1106 52-53 0.5227424478851219 1106 54-55 0.5418385550424603 1106 56-57 0.5773549165135989 1106 58-59 0.5259431469093201 1106 60-61 0.475315697931201 1106 62-63 0.5580923865215297 1106 64-65 0.5557571798257683 1106 66-67 0.5948119198993069 1106 68-69 0.6670450492778386 1106 70-71 0.6035586791820009 1106 72-73 0.5036277123901556 1106 74-75 0.6168595469186329 1106 76-77 0.5681302604052121 1106 78-79 0.5057644960252787 1106 80-81 0.5889847811899145 1106 82-83 0.45195040533803166 1106 84-85 0.5029508639573095 1106 86-87 0.6354914800785139 1106 88-89 0.643332911124844 1106 90-91 0.6409109827968003 1106 92-93 0.6492141898542378 1106 94-95 0.6452277893055722 1106 96-97 0.5935296645199912 1106 98-99 0.5708861795222795 1106 100 0.6256999415203417 1107 1 0.3737329377584828 1107 2 0.34853288550032957 1107 3 0.4068629587992838 1107 4 0.2920568498030107 1107 5 0.30499766909281334 1107 6 0.31684835543545375 1107 7 0.27509776437995725 1107 8 0.29279281074987296 1107 9 0.22944706606153886 1107 10-11 0.35854163829387886 1107 12-13 0.3463167289763902 1107 14-15 0.440205057541597 1107 16-17 0.39468174517710963 1107 18-19 0.41440162953212223 1107 20-21 0.3731619807927089 1107 22-23 0.5050348540375467 1107 24-25 0.4727434025519486 1107 26-27 0.5249357696959507 1107 28-29 0.520080636435658 1107 30-31 0.5396787806920784 1107 32-33 0.5023616191957885 1107 34-35 0.537061087590466 1107 36-37 0.6698520925912135 1107 38-39 0.5890014873312523 1107 40-41 0.6179146034674119 1107 42-43 0.6408574714569326 1107 44-45 0.5843400124239864 1107 46-47 0.5792720383138956 1107 48-49 0.6029557887431309 1107 50-51 0.506230422378863 1107 52-53 0.563770122836651 1107 54-55 0.5258147972815266 1107 56-57 0.5366087896357925 1107 58-59 0.4764176166196421 1107 60-61 0.4811523119116501 1107 62-63 0.49691933483420314 1107 64-65 0.5168276500844939 1107 66-67 0.48933299438468225 1107 68-69 0.4811596662745288 1107 70-71 0.4989023636914709 1107 72-73 0.5312708367413705 1107 74-75 0.4721695435890183 1107 76-77 0.4788134184442612 1107 78-79 0.43266115074451506 1107 80-81 0.4738279466716335 1107 82-83 0.5003999323372668 1107 84-85 0.43593875004540195 1107 86-87 0.4475765027002865 1107 88-89 0.39327904975422 1107 90-91 0.41465650251005925 1107 92-93 0.4103989931133398 1107 94-95 0.41606357088302204 1107 96-97 0.38022639562444027 1107 98-99 0.3939491287750734 1107 100 0.6586753415634305 1108 1 0.3158312147399407 1108 2 0.31240621171012606 1108 3 0.2913626106445051 1108 4 0.24839377485293568 1108 5 0.23349183816875296 1108 6 0.24672043663092325 1108 7 0.2538648686199636 1108 8 0.237258647193066 1108 9 0.1739035435052756 1108 10-11 0.2295570442765822 1108 12-13 0.2213498865744512 1108 14-15 0.25835568716160395 1108 16-17 0.2913704910604835 1108 18-19 0.25525872937606664 1108 20-21 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50-51 -0.07212605737117173 2316 52-53 -0.5390255511552766 2316 54-55 -0.4403670140332778 2316 56-57 -0.2651775720454026 2316 58-59 -0.45843157591757944 2316 60-61 -0.3791816113134061 2316 62-63 -0.5262805835154793 2316 64-65 -0.30038009079457595 2316 66-67 -0.19305795044492413 2316 68-69 -0.08936739293053364 2316 70-71 -0.21921336765329613 2316 72-73 -0.2855079444433315 2316 74-75 -0.1056912119525748 2316 76-77 -0.09144088047939647 2316 78-79 -0.426924453551905 2316 80-81 -0.01563206738677536 2316 82-83 0.00921389860558719 2316 84-85 -0.13845592990324462 2316 86-87 0.027364921984389667 2316 88-89 -0.08288855817205132 2316 90-91 0.03783176739342409 2316 92-93 0.055037375543061984 2316 94-95 0.016615188440376016 2316 96-97 0.022884570731417142 2316 98-99 0.17125687698623793 2316 100 -0.1270519151464029 >>END_MODULE >>Per sequence quality scores pass #Quality Count 0 18.0 1 61.0 2 105.0 3 95.0 4 101.0 5 133.0 6 178.0 7 758.0 8 1924.0 9 2989.0 10 5061.0 11 8448.0 12 12805.0 13 15876.0 14 17503.0 15 19328.0 16 21733.0 17 25482.0 18 30115.0 19 35515.0 20 41250.0 21 48761.0 22 55804.0 23 59570.0 24 60486.0 25 62897.0 26 68386.0 27 76807.0 28 89148.0 29 105144.0 30 126403.0 31 154885.0 32 194217.0 33 253534.0 34 346899.0 35 498071.0 36 765803.0 37 1236380.0 38 1332829.0 39 343490.0 40 552.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.16009595199454 18.405907054894133 12.216201351872268 24.21779564123906 2 15.78140801422809 20.444503480954783 40.136247832071376 23.637840672745757 3 18.176392683509714 25.949141964352556 30.491315353886783 25.383149998250943 4 12.127817614325334 16.61785586198802 38.59523942631448 32.659087097372165 5 12.62870879859583 37.68026750867104 35.8097042670523 13.881319425680838 6 30.532304709095104 37.39792864331971 18.470574835224078 13.599191812361108 7 26.664117334640686 32.52625730277403 23.268766597213713 17.540858765371574 8 25.458422976262362 35.580146702542905 21.04945348114169 17.911976840053047 9 26.416518566636842 14.883444255148772 20.60026065605249 38.099776522161896 10-11 24.23303875301632 25.913197348548362 29.822849797375937 20.030914101059384 12-13 24.806392719361188 23.983810488716053 29.385072634547 21.824724157375762 14-15 22.2703018186656 25.42540241453625 26.792645905607998 25.511649861190154 16-17 21.860774066466618 28.17751244015256 27.578721445583437 22.38299204779738 18-19 21.64544782659433 27.50651225749216 29.248012000401406 21.6000279155121 20-21 22.39680411299491 26.885062738993625 29.144956069955956 21.573177078055505 22-23 22.20857728216391 26.71587618975762 28.96984444632093 22.105702081757542 24-25 21.93948629642196 26.809301814744284 29.116728347967523 22.13448354086623 26-27 21.858287546535838 26.96954047596479 28.997898328795515 22.17427364870386 28-29 21.893574496585604 27.00793048535453 29.113327849378397 21.985167168681468 30-31 22.231636118898823 26.914534893032965 29.06503008687915 21.78879890118906 32-33 21.898323578945167 27.078315576685704 29.03250296815833 21.990857876210796 34-35 22.09878470250096 27.120714975103194 28.9689707277647 21.81152959463115 36-37 22.195440454429594 27.034505629987237 28.843361881332108 21.92669203425106 38-39 21.980372969798623 27.27259726031607 28.822415780077364 21.92461398980795 40-41 22.07751683551353 27.088532922645804 28.903731379756124 21.930218862084537 42-43 21.91443904728409 27.195590742763386 28.755516210021348 22.13445399993118 44-45 21.93911146610414 27.337989837797753 28.714065538063466 22.008833158034633 46-47 21.94402148081365 27.194467526744777 28.483072810256083 22.37843818218549 48-49 21.927242949600963 27.276804950611478 28.532950374823585 22.263001724963978 50-51 21.91461781605039 27.52914388928889 28.42143609574233 22.134802198918386 52-53 22.011959460193882 27.534144463983058 28.25053687137913 22.20335920444394 54-55 21.93867143175183 27.480068926355305 28.336336030039387 22.244923611853476 56-57 21.995383834496664 27.54192496700258 28.18315077526263 22.279540423238124 58-59 21.97030875239371 27.456742047284973 28.397160217778637 22.17578898254268 60-61 22.090115356541837 27.56648425261879 28.314605511062407 22.028794879776967 62-63 21.905551292777226 27.578863261170937 28.41013228528391 22.105453160767926 64-65 21.933262640001974 27.372262833381527 28.357076408462735 22.33739811815376 66-67 21.887035341725046 27.473869267309762 28.23092459373941 22.408170797225775 68-69 21.792235174830793 27.47819268570145 28.343505544009425 22.38606659545833 70-71 21.834542230080995 27.34813643313118 28.31850985274827 22.498811484039553 72-73 21.77533684922952 27.561411023086556 28.16399595879012 22.4992561688938 74-75 21.813340709581325 27.573320673389972 28.13321745823191 22.48012115879679 76-77 21.86124952364576 27.488166841789592 28.15407798863811 22.49650564592654 78-79 21.822506456312084 27.4049425416418 28.175965374129415 22.5965856279167 80-81 21.79657618563533 27.581752024477694 27.97428661816708 22.64738517171989 82-83 21.936496059626737 27.570330068879915 28.01586838497897 22.477305486514375 84-85 21.911459210141363 27.58311527202773 27.967665907742497 22.537759610088408 86-87 21.739850666801782 27.54795019284683 28.145797843237325 22.566401297114062 88-89 21.757216280813076 27.34314150036991 28.209870726919085 22.68977149189793 90-91 22.009611181066695 27.422825030785315 28.09821813355045 22.469345654597536 92-93 21.873079402565068 27.39633350594192 28.10849103481463 22.622096056678384 94-95 21.774119339192826 27.28351536993679 28.133697815152146 22.808667475718238 96-97 21.852048155043274 27.22998836508624 28.15787232908942 22.76009115078106 98-99 21.851998586552572 27.179631385724377 28.046577407657658 22.921792620065396 100 21.963811236169605 27.16094552263731 28.05650143676399 22.818741804429095 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2154.0 1 1620.0 2 2312.0 3 3442.0 4 2509.5 5 1928.5 6 2831.5 7 5080.5 8 7740.5 9 8884.0 10 7298.0 11 5463.0 12 5388.0 13 5609.5 14 5872.5 15 6344.0 16 6918.5 17 7386.5 18 8041.5 19 8893.0 20 9952.0 21 11766.0 22 14395.5 23 18115.0 24 22798.0 25 28686.0 26 36136.5 27 44248.0 28 53053.5 29 63344.0 30 75259.0 31 87007.5 32 99067.0 33 112463.5 34 126756.0 35 139564.5 36 154648.0 37 169614.0 38 180074.0 39 189553.5 40 194926.0 41 198871.0 42 203580.5 43 208851.0 44 214710.0 45 221265.5 46 227846.0 47 231648.0 48 235914.0 49 238412.5 50 236345.5 51 229683.5 52 219351.5 53 208998.5 54 197598.5 55 182128.0 56 163599.0 57 145151.0 58 126709.5 59 108176.5 60 89152.5 61 71219.0 62 56652.5 63 43968.5 64 32270.0 65 23351.0 66 17148.0 67 12780.5 68 9657.5 69 7158.0 70 5408.5 71 4073.0 72 3051.5 73 2238.0 74 1651.5 75 1217.0 76 864.5 77 627.0 78 438.0 79 313.5 80 264.0 81 219.0 82 172.5 83 131.5 84 109.5 85 101.5 86 86.5 87 69.5 88 50.0 89 36.0 90 31.0 91 29.5 92 24.0 93 16.0 94 15.5 95 12.5 96 4.5 97 4.0 98 7.5 99 6.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.16731638827991105 2 0.10729557627169606 3 0.03212657675147037 4 0.03343386369964821 5 0.028776653946764662 6 0.002827008025434575 7 0.004804279534553555 8 0.005866450179948048 9 0.006029861048470278 10-11 0.005392558661233582 12-13 0.008505535706582058 14-15 0.018007877711149717 16-17 0.00894674505159208 18-19 0.01768105597410526 20-21 0.01928248248562311 22-23 0.047544392196542745 24-25 0.016275722504814086 26-27 0.009870016458742678 28-29 0.02772265384479628 30-31 0.015197210772567369 32-33 0.020785862476027626 34-35 0.021063660952515415 36-37 0.022362777357267143 38-39 0.02037733530472205 40-41 0.02733863830376904 42-43 0.036481476397587795 44-45 0.03866301149235956 46-47 0.021897056381978787 48-49 0.014143210670598986 50-51 0.013211768720022277 52-53 0.02383347517396721 54-55 0.027943258517301287 56-57 0.020630622150931508 58-59 0.02746119645516071 60-61 0.027542901889421826 62-63 0.05381119900437026 64-65 0.033352158265387094 66-67 0.022305583553284363 68-69 0.029650902093358588 70-71 0.04366338406913979 72-73 0.04189854668909971 74-75 0.04269925994485864 76-77 0.023866157347671658 78-79 0.018187629666524173 80-81 0.024258343432125008 82-83 0.019854420525450917 84-85 0.020164901175643152 86-87 0.02200327344651824 88-89 0.01862066846810808 90-91 0.02898091753241745 92-93 0.02706083982728125 94-95 0.020736839215470957 96-97 0.01455990838533067 98-99 0.019119071617100882 100 0.043679725155992014 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6119544.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 65.25473401909665 #Duplication Level Percentage of deduplicated Percentage of total 1 77.36716011032178 50.48573454811911 2 14.565946738176782 19.009939602721087 3 4.083584459156113 7.994196531802462 4 1.571539280562983 4.102015110147998 5 0.7558867037597059 2.4662592901205653 6 0.42245936180557153 1.6540483973099933 7 0.2818999586656378 1.2876714775904372 8 0.1787885054487151 0.9333437094982171 9 0.13840900864509142 0.8128658740484058 >10 0.5897467374031634 6.541348354067877 >50 0.029799022693597146 1.3270611262387713 >100 0.013658377091831975 1.6638242076439897 >500 7.371415843516636E-4 0.3128927904444438 >1k 2.884460135541185E-4 0.4324148290677365 >5k 0.0 0.0 >10k+ 9.61486711838753E-5 0.9763841511788953 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18793 0.3070980452138264 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16974 0.2773736082296328 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10919 0.17842832733942268 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 1.6341086852222976E-5 3.268217370444595E-5 10-11 1.6341086852222976E-5 0.0 0.0 3.268217370444595E-5 3.268217370444595E-5 12-13 1.6341086852222976E-5 0.0 0.0 3.268217370444595E-5 3.268217370444595E-5 14-15 1.6341086852222976E-5 0.0 0.0 3.268217370444595E-5 6.53643474088919E-5 16-17 1.6341086852222976E-5 0.0 0.0 4.085271713055744E-5 8.170543426111488E-5 18-19 1.6341086852222976E-5 0.0 0.0 4.902326055666893E-5 8.170543426111488E-5 20-21 1.6341086852222976E-5 8.170543426111488E-6 0.0 6.53643474088919E-5 1.0621706453944934E-4 22-23 1.6341086852222976E-5 1.6341086852222976E-5 0.0 1.7975195537445275E-4 1.5524032509611828E-4 24-25 1.6341086852222976E-5 1.6341086852222976E-5 0.0 3.0231010676612505E-4 1.7158141194834124E-4 26-27 3.268217370444595E-5 1.6341086852222976E-5 0.0 5.229147792711352E-4 1.8792249880056423E-4 28-29 3.268217370444595E-5 1.6341086852222976E-5 0.0 0.0018138606405967504 1.960930422266757E-4 30-31 3.268217370444595E-5 3.268217370444595E-5 0.0 0.006185101373566397 1.960930422266757E-4 32-33 3.268217370444595E-5 4.902326055666893E-5 0.0 0.015899877507212955 2.124341290788987E-4 34-35 3.268217370444595E-5 5.7193803982780415E-5 0.0 0.030606855674213635 2.124341290788987E-4 36-37 3.268217370444595E-5 6.53643474088919E-5 0.0 0.053459865637047466 2.124341290788987E-4 38-39 3.268217370444595E-5 6.53643474088919E-5 0.0 0.09017011725056638 2.124341290788987E-4 40-41 3.268217370444595E-5 6.53643474088919E-5 0.0 0.13210134611337054 2.2877521593112166E-4 42-43 3.268217370444595E-5 6.53643474088919E-5 0.0 0.17281516400568409 2.3694575935723314E-4 44-45 3.268217370444595E-5 6.53643474088919E-5 0.0 0.22444482791528259 2.4511630278334465E-4 46-47 3.268217370444595E-5 6.53643474088919E-5 0.0 0.28882053956961495 2.614573896355676E-4 48-49 3.268217370444595E-5 6.53643474088919E-5 0.0 0.3571753058724637 2.859690199139021E-4 50-51 3.268217370444595E-5 6.53643474088919E-5 0.0 0.43164163865804384 2.941395633400136E-4 52-53 3.268217370444595E-5 6.53643474088919E-5 0.0 0.5034362037432855 3.34992280470571E-4 54-55 3.268217370444595E-5 6.53643474088919E-5 0.0 0.5914329564425062 3.7584499760112846E-4 56-57 3.268217370444595E-5 6.53643474088919E-5 0.0 0.6952151990409743 3.7584499760112846E-4 58-59 3.268217370444595E-5 6.53643474088919E-5 0.0 0.8125523731833614 3.8401554102723996E-4 60-61 3.268217370444595E-5 6.53643474088919E-5 0.0 0.9380192380347294 3.921860844533514E-4 62-63 4.902326055666893E-5 6.53643474088919E-5 0.0 1.0673589404700743 3.921860844533514E-4 64-65 4.902326055666893E-5 6.53643474088919E-5 0.0 1.2030308140606554 4.0035662787946287E-4 66-67 4.902326055666893E-5 6.53643474088919E-5 0.0 1.3593414803455945 4.166977147316859E-4 68-69 4.902326055666893E-5 6.53643474088919E-5 0.0 1.5432849244976423 4.248682581577974E-4 70-71 4.902326055666893E-5 6.53643474088919E-5 0.0 1.748316541232484 4.248682581577974E-4 72-73 4.902326055666893E-5 6.53643474088919E-5 0.0 1.9526700028629587 5.392558661233582E-4 74-75 4.902326055666893E-5 6.53643474088919E-5 0.0 2.1608472788168527 5.392558661233582E-4 76-77 4.902326055666893E-5 6.53643474088919E-5 0.0 2.3892629908372256 5.392558661233582E-4 78-79 4.902326055666893E-5 6.53643474088919E-5 0.0 2.654487000992231 5.392558661233582E-4 80-81 4.902326055666893E-5 6.53643474088919E-5 0.0 2.9378577880966295 5.637674964016927E-4 82-83 4.902326055666893E-5 6.53643474088919E-5 0.0 3.2358865301074724 6.37302387236696E-4 84-85 4.902326055666893E-5 6.53643474088919E-5 0.0 3.5360069312353994 6.37302387236696E-4 86-87 4.902326055666893E-5 6.53643474088919E-5 0.0 3.8513980780267287 6.37302387236696E-4 88 4.902326055666893E-5 6.53643474088919E-5 0.0 4.109603591378704 6.37302387236696E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9440 0.0 49.178078 1 GTATCAA 15775 0.0 36.26382 1 TACCTGG 5670 0.0 36.154785 2 GTACCTG 6375 0.0 32.86597 1 TCAACGC 18720 0.0 29.544304 4 ATCAACG 18970 0.0 29.20473 3 CAACGCA 18960 0.0 29.119093 5 AACGCAG 19730 0.0 28.190779 6 TATCAAC 20270 0.0 27.67254 2 ACCTGGG 7450 0.0 27.121824 3 GTACATG 17695 0.0 26.182503 1 TACATGG 18120 0.0 25.091734 2 ACGCAGA 22455 0.0 24.666124 7 ACATGGG 17920 0.0 24.255508 3 CGCAGAG 23095 0.0 23.902393 8 CATGGGG 11340 0.0 21.298704 4 GCAGAGT 25920 0.0 20.591413 9 GAGTACT 16415 0.0 19.42178 12-13 AGAGTAC 23580 0.0 19.237774 10-11 CAGAGTA 25090 0.0 18.875835 10-11 >>END_MODULE