FastQCFastQC Report
Mon 6 Jun 2016
ERR1211524_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211524_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6119544
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT212070.34654542887509265No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT191740.31332399930452337No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT136050.22232048662449358No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA101800.047.6759571
GTATCAA167150.035.029221
TACCTGG55650.033.547832
GTACCTG61750.030.6927341
TCAACGC204700.027.8464374
ATCAACG206750.027.6162413
CAACGCA209800.027.2815155
TATCAAC218950.026.3535232
AACGCAG218450.026.3085886
ACCTGGG69500.025.9645163
GTACATG184350.025.7914351
TACATGG188350.024.5179942
ACATGGG185650.024.2242743
TATAACG6950.023.005582
ACGCAGA248700.022.995057
TAACGCA6750.022.9740124
CGCAGAG251800.022.5439918
CATGGGG107400.021.8772344
ATAACGC7950.020.688773
AGAGTAC258200.019.5007810-11
Produced by FastQC (version 0.11.5)