##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211524_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 6119544 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1189703023624 33.0 31.0 34.0 30.0 34.0 2 32.3322386439251 34.0 31.0 34.0 30.0 34.0 3 32.349222589134094 34.0 31.0 34.0 30.0 34.0 4 35.98468137495212 37.0 35.0 37.0 35.0 37.0 5 35.90820835670109 37.0 35.0 37.0 35.0 37.0 6 35.92818468173446 37.0 35.0 37.0 35.0 37.0 7 35.854169853178604 37.0 35.0 37.0 35.0 37.0 8 35.851942563040645 37.0 35.0 37.0 35.0 37.0 9 37.5065285910192 39.0 37.0 39.0 35.0 39.0 10-11 37.47025350254856 39.0 37.0 39.0 34.5 39.0 12-13 37.384244969886645 39.0 37.0 39.0 34.0 39.0 14-15 38.62974937348273 40.0 38.0 41.0 34.0 41.0 16-17 38.56086433891153 40.0 38.0 41.0 34.0 41.0 18-19 38.54458837129041 40.0 38.0 41.0 34.0 41.0 20-21 38.48269045863548 40.0 38.0 41.0 34.0 41.0 22-23 38.372263685006594 40.0 38.0 41.0 34.0 41.0 24-25 38.262829959225726 40.0 38.0 41.0 34.0 41.0 26-27 38.08890932069448 40.0 37.5 41.0 33.0 41.0 28-29 38.12457987065703 40.0 38.0 41.0 33.0 41.0 30-31 38.050969974233375 40.0 37.5 41.0 33.0 41.0 32-33 37.873665014909605 40.0 37.0 41.0 33.0 41.0 34-35 37.756615443895825 40.0 37.0 41.0 33.0 41.0 36-37 37.70218148607151 40.0 37.0 41.0 33.0 41.0 38-39 37.608779183547014 40.0 37.0 41.0 32.5 41.0 40-41 37.4508214174128 40.0 37.0 41.0 32.0 41.0 42-43 37.302920364654625 40.0 36.0 41.0 31.0 41.0 44-45 37.12193710838585 40.0 36.0 41.0 31.0 41.0 46-47 36.87232357508991 39.0 36.0 41.0 30.0 41.0 48-49 36.65813980584174 39.0 35.5 41.0 30.0 41.0 50-51 36.46427503421823 39.0 35.0 41.0 29.5 41.0 52-53 36.266479169036124 39.0 35.0 40.0 29.0 41.0 54-55 36.07165705483938 39.0 35.0 40.0 28.0 41.0 56-57 35.87359956885676 38.0 35.0 40.0 28.0 41.0 58-59 35.616167234029206 38.0 34.0 40.0 27.5 41.0 60-61 35.30358242378844 38.0 34.0 40.0 26.5 41.0 62-63 34.98140302610783 37.5 34.0 40.0 26.0 41.0 64-65 34.66755480473709 37.0 33.0 40.0 26.0 41.0 66-67 34.32339337702287 36.5 33.0 39.0 26.0 41.0 68-69 33.92640758853928 36.0 32.5 39.0 25.5 40.0 70-71 33.538973982375154 35.5 32.0 38.5 25.0 40.0 72-73 33.13203312207576 35.0 32.0 38.0 24.0 40.0 74-75 32.724585769789385 35.0 31.0 37.0 23.5 39.0 76-77 31.171930457563505 33.5 29.5 35.5 21.5 38.5 78-79 32.003380725753416 34.0 31.0 36.0 23.0 39.0 80-81 31.9716284415963 34.0 31.0 36.0 23.0 37.5 82-83 31.687570430084335 34.0 31.0 35.5 22.0 37.0 84-85 31.38491487274215 34.0 31.0 35.0 21.0 37.0 86-87 31.087932041995288 34.0 30.0 35.0 20.0 36.0 88-89 30.807291441976723 34.0 30.0 35.0 20.0 36.0 90-91 30.519657592134315 34.0 29.5 35.0 19.5 35.5 92-93 30.30440707673644 34.0 29.0 35.0 19.0 35.0 94-95 30.08079401014193 34.0 29.0 35.0 18.0 35.0 96-97 29.8720645688633 34.0 29.0 35.0 18.0 35.0 98-99 29.70059362266208 34.0 29.0 35.0 18.0 35.0 100 27.81871917253965 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2904998549992186 1101 2 0.18022784117351875 1101 3 0.23253725348418897 1101 4 0.18486342348931828 1101 5 0.16898500716239084 1101 6 0.1993639402690306 1101 7 0.21021176661501073 1101 8 0.1677645417777356 1101 9 0.1770057607823503 1101 10-11 0.18720600080163763 1101 12-13 0.2032103378761363 1101 14-15 0.26948330187046565 1101 16-17 0.2987885330295228 1101 18-19 0.3078850695094033 1101 20-21 0.21752171476167348 1101 22-23 0.2523848457446647 1101 24-25 0.2758873698401558 1101 26-27 0.1941320534762312 1101 28-29 0.2936803629363922 1101 30-31 0.3197199845012193 1101 32-33 0.26516731636691304 1101 34-35 0.2531114616714163 1101 36-37 0.3164686762938942 1101 38-39 0.28413513003319935 1101 40-41 0.28374006329341483 1101 42-43 0.2854915328382859 1101 44-45 0.3032263576667944 1101 46-47 0.3462895090137934 1101 48-49 0.3561216272564778 1101 50-51 0.3079006029548097 1101 52-53 0.27027953677988137 1101 54-55 0.24442972042785271 1101 56-57 0.2605529249875147 1101 58-59 0.34405665321326495 1101 60-61 0.2982458131786174 1101 62-63 0.2277140674781819 1101 64-65 0.19109326729395804 1101 66-67 0.12557730257429256 1101 68-69 0.1284306668905444 1101 70-71 0.09582661377148582 1101 72-73 -0.007246059687261663 1101 74-75 0.07388200353743457 1101 76-77 0.17432589229162332 1101 78-79 0.22656704471933864 1101 80-81 0.2541214194431518 1101 82-83 0.1495677211953037 1101 84-85 0.0981092278990019 1101 86-87 0.0815978037288474 1101 88-89 0.1628979266429731 1101 90-91 0.0613712044232777 1101 92-93 -0.039674502688018976 1101 94-95 0.05087944225340735 1101 96-97 -0.04049908841426486 1101 98-99 -0.09377772778338667 1101 100 0.03445846033591238 1102 1 0.3043910993857679 1102 2 0.22630625333145815 1102 3 0.33209085582769404 1102 4 0.190588436958123 1102 5 0.20106523025886958 1102 6 0.14555040770632388 1102 7 0.14525022619358197 1102 8 0.12924869258127103 1102 9 0.21686289349784005 1102 10-11 0.19936546443631187 1102 12-13 0.2417714714243644 1102 14-15 0.31192763118950495 1102 16-17 0.32268629078448186 1102 18-19 0.337468915136256 1102 20-21 0.31901826513716713 1102 22-23 0.28670570241701654 1102 24-25 0.3287315407129654 1102 26-27 0.29679738436884406 1102 28-29 0.2784300762480072 1102 30-31 0.36417322304555455 1102 32-33 0.4325790788439008 1102 34-35 0.388508396054732 1102 36-37 0.4402035562854607 1102 38-39 0.3928021237837598 1102 40-41 0.3958911102885381 1102 42-43 0.3763336192589932 1102 44-45 0.4019097034345478 1102 46-47 0.4165720859819544 1102 48-49 0.3960125136364141 1102 50-51 0.46109999303698146 1102 52-53 0.47149839249145487 1102 54-55 0.4124666443235796 1102 56-57 0.45053708036412843 1102 58-59 0.43237603430606697 1102 60-61 0.41312279522644957 1102 62-63 0.43370693260681037 1102 64-65 0.3354218785985168 1102 66-67 0.3999728833267753 1102 68-69 0.4210047409404183 1102 70-71 0.27962004221610925 1102 72-73 0.38252981380313145 1102 74-75 0.36783376062253836 1102 76-77 0.41814742556163154 1102 78-79 0.2851858142828547 1102 80-81 0.40309115960962316 1102 82-83 0.25401847938971045 1102 84-85 0.27180915651544524 1102 86-87 0.31108627892121277 1102 88-89 0.29922966331909606 1102 90-91 0.2790048482467711 1102 92-93 0.23263196577713074 1102 94-95 0.2682448206624777 1102 96-97 0.21463840441580473 1102 98-99 0.2404241417776447 1102 100 0.43050351080744065 1103 1 0.23133636030331672 1103 2 0.21148736530849277 1103 3 0.2549875529687 1103 4 0.12473683682467396 1103 5 0.13283449561768634 1103 6 0.1478931470736029 1103 7 0.1558071245891668 1103 8 0.20743336627114672 1103 9 0.20416065981202536 1103 10-11 0.17951389331348366 1103 12-13 0.21921693475692194 1103 14-15 0.3067590267453397 1103 16-17 0.3267136570290461 1103 18-19 0.2797145744223499 1103 20-21 0.23145215059533086 1103 22-23 0.2679006234487957 1103 24-25 0.1751449710472599 1103 26-27 0.25412101628738526 1103 28-29 0.3505509700915681 1103 30-31 0.35437815010362783 1103 32-33 0.36469030687659654 1103 34-35 0.3873057410556058 1103 36-37 0.42512410015545044 1103 38-39 0.34422288589272654 1103 40-41 0.44933569275320195 1103 42-43 0.4392289054289691 1103 44-45 0.4051372666875821 1103 46-47 0.469616157068522 1103 48-49 0.4733697912799144 1103 50-51 0.38695214700952363 1103 52-53 0.3772072208184838 1103 54-55 0.5306122921372989 1103 56-57 0.5137249122555048 1103 58-59 0.48416915430586016 1103 60-61 0.45114642041980346 1103 62-63 0.49140351581520747 1103 64-65 0.442206044866289 1103 66-67 0.4042152248529689 1103 68-69 0.5184739270475021 1103 70-71 0.5072852939557535 1103 72-73 0.41977809723159965 1103 74-75 0.4393680795257282 1103 76-77 0.4994143456756177 1103 78-79 0.5338773034902431 1103 80-81 0.4625336216617555 1103 82-83 0.4723337404259418 1103 84-85 0.4737398132235562 1103 86-87 0.4340729419483651 1103 88-89 0.44140849928118797 1103 90-91 0.4508707783074435 1103 92-93 0.46704693367424355 1103 94-95 0.5335579771101493 1103 96-97 0.5451365101542365 1103 98-99 0.5166252803125815 1103 100 0.5620251969046279 1104 1 0.24399609435467795 1104 2 0.22328931963003384 1104 3 0.2864666137161862 1104 4 0.07120154187138894 1104 5 0.12726989670193234 1104 6 0.18352782370433118 1104 7 0.18171917534088067 1104 8 0.17451258827861693 1104 9 0.2096198445715629 1104 10-11 0.25431098246653505 1104 12-13 0.25796190461407775 1104 14-15 0.3843669356091226 1104 16-17 0.28104241452348333 1104 18-19 0.2719381136879875 1104 20-21 0.15785397641283083 1104 22-23 0.2895568681677716 1104 24-25 0.3461219102615374 1104 26-27 0.377663333782742 1104 28-29 0.4521344631860984 1104 30-31 0.3594573291026393 1104 32-33 0.36621900763463344 1104 34-35 0.38385356319870567 1104 36-37 0.3702641013631691 1104 38-39 0.4305598058705016 1104 40-41 0.521483015824451 1104 42-43 0.5849316223034506 1104 44-45 0.5243083721308466 1104 46-47 0.45533534096789907 1104 48-49 0.44927111163946165 1104 50-51 0.4388083962465572 1104 52-53 0.378816692757205 1104 54-55 0.42898323831894203 1104 56-57 0.45745117343498265 1104 58-59 0.4801784801896076 1104 60-61 0.3930154223152158 1104 62-63 0.4589083911927787 1104 64-65 0.6055283914944383 1104 66-67 0.5530549631877975 1104 68-69 0.571095042547789 1104 70-71 0.43468316733973467 1104 72-73 0.6327413775689621 1104 74-75 0.6473593761784997 1104 76-77 0.5400395452413633 1104 78-79 0.6544901478999918 1104 80-81 0.6783257287849551 1104 82-83 0.4940455268711581 1104 84-85 0.6238097671263141 1104 86-87 0.6645751460230436 1104 88-89 0.6530715667049769 1104 90-91 0.5261646476911999 1104 92-93 0.4886927086583519 1104 94-95 0.57930805758809 1104 96-97 0.48392618273825505 1104 98-99 0.47457534966914494 1104 100 0.7587636138372744 1105 1 0.2327906597999032 1105 2 0.1404205877216924 1105 3 0.18598113814067574 1105 4 0.12335677625951291 1105 5 0.11861448648590311 1105 6 0.14039368693273957 1105 7 0.16098014379752357 1105 8 0.1772424248019675 1105 9 0.1330263500308959 1105 10-11 0.19845106274107138 1105 12-13 0.2186223592721248 1105 14-15 0.3003025306263538 1105 16-17 0.27613343794583756 1105 18-19 0.3135569690223292 1105 20-21 0.23169684689435854 1105 22-23 0.28554179893661313 1105 24-25 0.3227186528546824 1105 26-27 0.27817821505993123 1105 28-29 0.3278407630220457 1105 30-31 0.3445669573695298 1105 32-33 0.3399673814430315 1105 34-35 0.4270644406232691 1105 36-37 0.41821466687075315 1105 38-39 0.47925330924587684 1105 40-41 0.41527074736729475 1105 42-43 0.33108861323835015 1105 44-45 0.37930231776105217 1105 46-47 0.45067048801372067 1105 48-49 0.48152760731132815 1105 50-51 0.5363751285671938 1105 52-53 0.5367506022517361 1105 54-55 0.5239814540554519 1105 56-57 0.5506323853136763 1105 58-59 0.5822787575299841 1105 60-61 0.5357255799713485 1105 62-63 0.5453039017779631 1105 64-65 0.6415332263288107 1105 66-67 0.5759108154854289 1105 68-69 0.5955336058518981 1105 70-71 0.6827484107531134 1105 72-73 0.596185369884509 1105 74-75 0.6613383010817913 1105 76-77 0.6257517088947004 1105 78-79 0.6474917208235311 1105 80-81 0.6821812536788912 1105 82-83 0.7059567848808577 1105 84-85 0.6090143684473617 1105 86-87 0.6456584451435567 1105 88-89 0.7394831876304018 1105 90-91 0.7733587201253691 1105 92-93 0.7248562649899775 1105 94-95 0.7649268938671945 1105 96-97 0.7966514026963729 1105 98-99 0.8244606284559879 1105 100 0.9220188726507565 1106 1 0.23425499781197345 1106 2 0.19113181442134675 1106 3 0.21134973896732845 1106 4 0.13815762953674238 1106 5 0.14896540810972425 1106 6 0.11157041727051364 1106 7 0.15869515959354175 1106 8 0.10076891438438906 1106 9 0.09139986337065409 1106 10-11 0.17172080614099627 1106 12-13 0.19063921701705056 1106 14-15 0.20581944843835487 1106 16-17 0.2384307927214948 1106 18-19 0.21422955797842747 1106 20-21 0.18219507897406828 1106 22-23 0.2749659342141726 1106 24-25 0.28518038038391325 1106 26-27 0.29074441060587475 1106 28-29 0.2861002300809403 1106 30-31 0.24388519600792335 1106 32-33 0.40156089614647783 1106 34-35 0.32866279316183267 1106 36-37 0.3419769603292835 1106 38-39 0.3373160160308615 1106 40-41 0.4170439438126152 1106 42-43 0.359051056702242 1106 44-45 0.47913015252137825 1106 46-47 0.4588006485064682 1106 48-49 0.4544901157125949 1106 50-51 0.42550631507060643 1106 52-53 0.44457239134160886 1106 54-55 0.33890422727138514 1106 56-57 0.33773598316944486 1106 58-59 0.48361776639068665 1106 60-61 0.5523832365759702 1106 62-63 0.5884706220179723 1106 64-65 0.6205564219624193 1106 66-67 0.5358497256413131 1106 68-69 0.5310856872574021 1106 70-71 0.5495715765155254 1106 72-73 0.5923532525036919 1106 74-75 0.4781085428761429 1106 76-77 0.3986981452934053 1106 78-79 0.6378229501856225 1106 80-81 0.6709779314823265 1106 82-83 0.6856813042630456 1106 84-85 0.6543625789353982 1106 86-87 0.5452786426181433 1106 88-89 0.6100443115666465 1106 90-91 0.6556686402848584 1106 92-93 0.6289762173259383 1106 94-95 0.8064437986917135 1106 96-97 0.6586192814193126 1106 98-99 0.6772690891477637 1106 100 0.795185609125113 1107 1 0.2530134442541865 1107 2 0.19582999488631714 1107 3 0.25528103144436187 1107 4 0.1799393636770148 1107 5 0.16173544943608675 1107 6 0.15223330384825573 1107 7 0.16060479000660877 1107 8 0.1817298991661005 1107 9 0.26667945284908257 1107 10-11 0.22486239299490052 1107 12-13 0.18881527514815843 1107 14-15 0.2617177581706116 1107 16-17 0.2567791582737229 1107 18-19 0.2307698952485353 1107 20-21 0.3075586432257893 1107 22-23 0.37168749051399885 1107 24-25 0.3827245467078271 1107 26-27 0.34141694326545036 1107 28-29 0.37251345451637263 1107 30-31 0.28338149426281234 1107 32-33 0.3229526739634281 1107 34-35 0.32705062440926014 1107 36-37 0.4587704460413349 1107 38-39 0.41340866738131155 1107 40-41 0.43670824057897306 1107 42-43 0.4443172787677767 1107 44-45 0.4815710519083396 1107 46-47 0.43683989875619034 1107 48-49 0.4539319160533992 1107 50-51 0.3587353532044588 1107 52-53 0.5014022579612671 1107 54-55 0.4327221660456999 1107 56-57 0.5585639303657146 1107 58-59 0.6636714319017827 1107 60-61 0.6472563685657136 1107 62-63 0.5999590075478665 1107 64-65 0.7023644992094162 1107 66-67 0.6329813628311243 1107 68-69 0.589202106161764 1107 70-71 0.7207050645778921 1107 72-73 0.6494169264367429 1107 74-75 0.7495208859436957 1107 76-77 0.7040137729252649 1107 78-79 0.7737164123945206 1107 80-81 0.6791269120334675 1107 82-83 0.6999802740419163 1107 84-85 0.4978940731477479 1107 86-87 0.5234660116370833 1107 88-89 0.5820853637240404 1107 90-91 0.506171509626963 1107 92-93 0.6150273464561273 1107 94-95 0.7134974348238821 1107 96-97 0.7535340335590774 1107 98-99 0.7690096927868311 1107 100 0.8580785523062637 1108 1 0.2444801759525248 1108 2 0.20953197118611655 1108 3 0.22923092496569097 1108 4 0.15203938410637363 1108 5 0.15093543146680588 1108 6 0.1229210512600929 1108 7 0.11269687400443473 1108 8 0.11813670117994235 1108 9 0.0805222687206637 1108 10-11 0.14247899810609255 1108 12-13 0.17376159192729546 1108 14-15 0.22817684222940215 1108 16-17 0.21390123652779636 1108 18-19 0.2411230878439099 1108 20-21 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-0.49463312257007175 2316 74-75 -0.5714008965192292 2316 76-77 -0.507186786936277 2316 78-79 -0.35467356042502374 2316 80-81 -0.35431907372010585 2316 82-83 -0.1798615730136035 2316 84-85 -0.2969374092479349 2316 86-87 -0.14534798141091088 2316 88-89 -0.25568231534383656 2316 90-91 -0.15948553999467308 2316 92-93 -0.010840465254279508 2316 94-95 -0.09031325139527624 2316 96-97 -0.15231785532260744 2316 98-99 -0.154598728813248 2316 100 -0.33831663026928993 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 8.0 11 25.0 12 71.0 13 168.0 14 564.0 15 1527.0 16 3507.0 17 7451.0 18 13098.0 19 20003.0 20 27674.0 21 37638.0 22 49061.0 23 56229.0 24 60573.0 25 66995.0 26 77806.0 27 92706.0 28 112245.0 29 135944.0 30 164294.0 31 198493.0 32 242898.0 33 304853.0 34 401198.0 35 548834.0 36 803634.0 37 1251379.0 38 1242380.0 39 198264.0 40 23.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.522410869647636 19.982573318220624 13.376432668034132 24.11858314409761 2 15.17100373023825 21.594630654738495 39.5988722451861 23.635493369837153 3 17.195919065685473 26.938460957718473 31.0616700991473 24.80394987744876 4 11.924291952882486 17.606954676748522 38.34077837630084 32.12797499406815 5 12.32692860784647 38.24666962990078 35.82412769795929 13.602274064293466 6 30.407869390807235 38.2471107154366 18.122143152684824 13.22287674107134 7 26.479848825337314 32.95716151399516 23.1655659310563 17.397423729611226 8 25.579702317698068 36.34045850650309 20.610503152475285 17.46933602332356 9 26.377648258259896 14.96705390159164 20.54105038270027 38.114247457448194 10-11 24.297537528940065 26.444102697848077 29.574850675148344 19.683509098063514 12-13 24.811946772504616 24.37638327300204 29.30938971923398 21.50228023525936 14-15 22.231591112017497 25.791611270382237 26.7664714887253 25.21032612887496 16-17 21.769585794146277 28.620033105616134 27.50776186082356 22.102619239414032 18-19 21.630758606422027 27.79437144158376 29.331693867002556 21.24317608499166 20-21 22.436005698968035 27.0812594827215 29.25489596245747 21.227838855852994 22-23 22.210265993675346 26.973292454470467 29.068456734684805 21.74798481716938 24-25 21.94336702211799 27.078046991736638 29.21786329177468 21.760722694370692 26-27 21.930845523060057 27.162161024908528 29.15601463935597 21.75097881267545 28-29 21.97696195211182 27.186182563877303 29.247842812929292 21.58901267108158 30-31 22.24966747449998 27.105442632927062 29.205972655573685 21.438917236999274 32-33 21.988499790909746 27.28225811591297 29.118166960733028 21.611075132444256 34-35 22.211202458578448 27.324508970253746 29.059280145787714 21.40500842538009 36-37 22.279897863728916 27.232335341377112 28.934933351679142 21.552833443214826 38-39 22.09761886767016 27.408412095016516 28.952510126502123 21.541458910811205 40-41 22.175993756092968 27.246618884632102 29.016820782889003 21.560566576385927 42-43 22.000238595424687 27.38806647007057 28.837590872581053 21.774104061923683 44-45 22.01923258412121 27.51529782936652 28.786736218757508 21.67873336775476 46-47 22.032805734985622 27.390385598899584 28.572634389110174 22.00417427700462 48-49 21.996808004371914 27.498195800136493 28.576630120724506 21.928366074767087 50-51 22.034283181685694 27.681649703153155 28.420880348621917 21.86318676653923 52-53 22.09005334336713 27.745741253007385 28.234594789369893 21.929610614255594 54-55 22.02649949557959 27.67549332250656 28.304860632158434 21.993146549755412 56-57 22.105130791695313 27.75233859208447 28.133463368144557 22.009067248075663 58-59 22.050836805109565 27.682386320368224 28.32772489086252 21.93905198365969 60-61 22.19132715595431 27.785474988151854 28.267375921295617 21.755821934598224 62-63 22.04760859963579 27.76740664654 28.319063664581677 21.86592108924253 64-65 22.002352068911225 27.548948256853507 28.278599482113414 22.170100192121854 66-67 21.946235396701542 27.64646034537126 28.187606978090933 22.21969727983626 68-69 21.860575222772002 27.69224646875897 28.238316522880584 22.208861785588443 70-71 21.878957037233622 27.596627391912442 28.21515186402626 22.30926370682767 72-73 21.804533457055577 27.83476707212502 28.027581910557515 22.33311756026189 74-75 21.82652341697736 27.831751066487893 27.970882694911335 22.370842821623416 76-77 21.85455722640684 27.67757590692766 28.066480383843494 22.401386482822 78-79 21.761659004852028 27.62455691941122 28.09486377876449 22.51892029697226 80-81 21.742876302670943 27.838225430045544 27.83612548457059 22.582772782712922 82-83 21.816694552890873 27.853814896759683 27.866195154538715 22.463295395810732 84-85 21.775918751261543 27.865447303626134 27.77816276715107 22.580471177961247 86-87 21.621235536065548 27.822582820045323 27.919931156616247 22.636250487272882 88-89 21.599584005287703 27.63929538484823 27.975693434844413 22.785427175019656 90-91 21.817101271992463 27.77044345990058 27.81955987118915 22.59289539691781 92-93 21.67119270937967 27.737550222237335 27.802126632534318 22.78913043584868 94-95 21.493365526237927 27.57665567596109 27.901840520884246 23.02813827691674 96-97 21.521255736270607 27.580452438958513 27.913884085593537 22.984407739177342 98-99 21.44639797910658 27.565807989628798 27.760349707656246 23.227444323608374 100 20.62973933997857 28.07294244245455 27.972263645082396 23.325054572484486 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2294.0 1 1748.0 2 2316.5 3 3319.5 4 2446.0 5 1960.5 6 2955.0 7 5370.0 8 8065.0 9 9286.0 10 7928.0 11 6155.0 12 6092.5 13 6477.0 14 6957.0 15 7399.0 16 7816.0 17 8427.0 18 9184.5 19 9930.5 20 11107.0 21 12901.0 22 15475.5 23 19223.5 24 24147.0 25 30127.0 26 37633.0 27 46123.5 28 54971.5 29 65469.5 30 77603.5 31 89122.5 32 101506.0 33 115728.0 34 129621.5 35 141579.5 36 156570.0 37 171432.5 38 181394.5 39 190903.5 40 196370.5 41 199738.0 42 204179.0 43 209700.5 44 215676.0 45 222044.0 46 227505.0 47 230661.0 48 234361.5 49 236138.5 50 234873.0 51 228258.0 52 217093.0 53 206787.5 54 195001.0 55 178673.5 56 160029.5 57 141931.0 58 123893.5 59 104571.0 60 85438.5 61 68657.5 62 54502.5 63 41997.0 64 30805.5 65 22154.5 66 16125.0 67 11904.5 68 8799.5 69 6528.0 70 4890.5 71 3596.0 72 2645.0 73 1909.5 74 1391.0 75 979.0 76 680.0 77 468.0 78 306.5 79 187.5 80 109.0 81 78.5 82 63.0 83 53.5 84 38.5 85 27.5 86 24.5 87 18.0 88 12.0 89 11.0 90 8.0 91 4.5 92 5.0 93 5.0 94 3.0 95 1.5 96 1.0 97 2.5 98 2.5 99 2.0 100 2.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0031374886756268114 2 0.07165566584699774 3 0.0010294884716900475 4 5.882791266800272E-4 5 0.0016994730326311894 6 5.882791266800272E-4 7 0.0 8 0.0010948528190989394 9 4.902326055666893E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.291318438105846E-4 18-19 0.0011928993402122773 20-21 0.003112977045348477 22-23 0.0 24-25 0.0 26-27 2.042635856527872E-4 28-29 9.559535808550441E-4 30-31 0.0014461861864217334 32-33 0.002892372372843467 34-35 0.003848325953698511 36-37 0.0011847287967861656 38-39 0.0013317985784561726 40-41 0.004877814425388558 42-43 0.006520093654036967 44-45 0.0047961089911274435 46-47 0.0065446052843153016 48-49 0.007974450383884812 50-51 0.007835551145640917 52-53 0.0037012561720285043 54-55 0.00236128705014622 56-57 0.002565550635799007 58-59 0.0032355351967401492 60-61 0.007255442562387001 62-63 0.0130156756777956 64-65 0.004093442256481856 66-67 0.009682093959942113 68-69 0.011961675575827219 70-71 0.004959519859649673 72-73 0.007533241038874792 74-75 0.0025982328095034532 76-77 0.0028596901991390206 78-79 0.007418853430909231 80-81 0.005515116812625255 82-83 0.015221722402845701 84-85 0.017141800107981903 86-87 0.021390482689559877 88-89 0.0195602809621109 90-91 0.023302389851269964 92-93 0.03243705740166261 94-95 0.03683280976491059 96-97 0.02203595562022268 98-99 0.0173542342370608 100 0.017583009452991922 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 6119544.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.0961212921359 #Duplication Level Percentage of deduplicated Percentage of total 1 76.37460699575614 48.95316073639197 2 15.123273280164948 19.386863169991415 3 4.304752287989578 8.277537743507388 4 1.6751184007171496 4.294743687642206 5 0.7904893797784052 2.533365158321097 6 0.4578826868301752 1.7609102539581596 7 0.2816816331348265 1.2638290086223738 8 0.19658163259051778 1.0080096133062342 9 0.13915791745587255 0.802753448041138 >10 0.6089691699608307 6.631277976640293 >50 0.031726814267031 1.3924433202773998 >100 0.01455151990841784 1.7256307109392344 >500 7.83750526579339E-4 0.3489263876820056 >1k 3.265622457585319E-4 0.49054766497607083 >5k 0.0 0.0 >10k+ 9.79686737272682E-5 1.1300011197030915 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21207 0.34654542887509265 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 19174 0.31332399930452337 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 13605 0.22232048662449358 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 1.6341086852222976E-5 7 0.0 0.0 0.0 0.0 1.6341086852222976E-5 8 0.0 0.0 0.0 0.0 1.6341086852222976E-5 9 0.0 0.0 0.0 3.268217370444595E-5 1.6341086852222976E-5 10-11 0.0 0.0 0.0 3.268217370444595E-5 1.6341086852222976E-5 12-13 0.0 0.0 0.0 4.085271713055744E-5 1.6341086852222976E-5 14-15 0.0 0.0 0.0 4.902326055666893E-5 1.6341086852222976E-5 16-17 0.0 0.0 0.0 5.7193803982780415E-5 1.6341086852222976E-5 18-19 0.0 0.0 0.0 6.53643474088919E-5 3.268217370444595E-5 20-21 0.0 0.0 0.0 8.170543426111488E-5 3.268217370444595E-5 22-23 0.0 0.0 0.0 1.7975195537445275E-4 5.7193803982780415E-5 24-25 0.0 0.0 0.0 3.1048065019223655E-4 8.170543426111488E-5 26-27 0.0 0.0 0.0 5.147442358450238E-4 8.170543426111488E-5 28-29 8.170543426111488E-6 0.0 0.0 0.0018138606405967504 8.170543426111488E-5 30-31 1.6341086852222976E-5 0.0 0.0 0.00620144246041862 8.170543426111488E-5 32-33 1.6341086852222976E-5 0.0 0.0 0.015899877507212955 1.1438760796556083E-4 34-35 1.6341086852222976E-5 0.0 0.0 0.03047612697939585 1.470697816700068E-4 36-37 1.6341086852222976E-5 0.0 8.170543426111488E-6 0.053394501289638574 1.470697816700068E-4 38-39 1.6341086852222976E-5 0.0 1.6341086852222976E-5 0.0901129234465836 1.470697816700068E-4 40-41 1.6341086852222976E-5 0.0 1.6341086852222976E-5 0.13206049339624 1.6341086852222976E-4 42-43 1.6341086852222976E-5 0.0 1.6341086852222976E-5 0.17270894694114464 1.7158141194834124E-4 44-45 1.6341086852222976E-5 0.0 1.6341086852222976E-5 0.22434678139416925 1.8792249880056423E-4 46-47 1.6341086852222976E-5 0.0 1.6341086852222976E-5 0.28884505119989334 1.960930422266757E-4 48-49 1.6341086852222976E-5 0.0 1.6341086852222976E-5 0.3574285927186731 2.4511630278334465E-4 50-51 1.6341086852222976E-5 0.0 1.6341086852222976E-5 0.4312657936604427 2.4511630278334465E-4 52-53 1.6341086852222976E-5 0.0 1.6341086852222976E-5 0.5029051184205882 3.1048065019223655E-4 54-55 1.6341086852222976E-5 0.0 1.6341086852222976E-5 0.5905260261222078 3.595039107489055E-4 56-57 1.6341086852222976E-5 0.0 1.6341086852222976E-5 0.6933604856832469 3.7584499760112846E-4 58-59 1.6341086852222976E-5 0.0 1.6341086852222976E-5 0.8096845124407963 3.8401554102723996E-4 60-61 1.6341086852222976E-5 0.0 1.6341086852222976E-5 0.9342362764284398 4.085271713055744E-4 62-63 3.268217370444595E-5 0.0 1.6341086852222976E-5 1.0622850330024591 4.4120934501002036E-4 64-65 3.268217370444595E-5 0.0 1.6341086852222976E-5 1.1972297282281164 4.493798884361318E-4 66-67 4.085271713055744E-5 0.0 1.6341086852222976E-5 1.352943944842949 4.902326055666892E-4 68-69 4.902326055666893E-5 0.0 1.6341086852222976E-5 1.5350163345504173 5.065736924189122E-4 70-71 4.902326055666893E-5 0.0 1.6341086852222976E-5 1.7376703231482606 5.229147792711352E-4 72-73 4.902326055666893E-5 0.0 1.6341086852222976E-5 1.9401690714210078 6.046202135322501E-4 74-75 4.902326055666893E-5 8.170543426111488E-6 1.6341086852222976E-5 2.1466795565159758 6.046202135322501E-4 76-77 4.902326055666893E-5 1.6341086852222976E-5 1.6341086852222976E-5 2.37360005908937 6.046202135322501E-4 78-79 4.902326055666893E-5 1.6341086852222976E-5 1.6341086852222976E-5 2.636479123281081 6.046202135322501E-4 80-81 4.902326055666893E-5 1.6341086852222976E-5 1.6341086852222976E-5 2.917169972141715 6.291318438105846E-4 82-83 4.902326055666893E-5 1.6341086852222976E-5 1.6341086852222976E-5 3.2115873339582164 6.781551043672535E-4 84-85 4.902326055666893E-5 1.6341086852222976E-5 1.6341086852222976E-5 3.5079492851101324 6.86325647793365E-4 86-87 4.902326055666893E-5 1.6341086852222976E-5 1.6341086852222976E-5 3.8193286950792413 6.86325647793365E-4 88 4.902326055666893E-5 1.6341086852222976E-5 1.6341086852222976E-5 4.0728524870480545 6.86325647793365E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10180 0.0 47.675957 1 GTATCAA 16715 0.0 35.02922 1 TACCTGG 5565 0.0 33.54783 2 GTACCTG 6175 0.0 30.692734 1 TCAACGC 20470 0.0 27.846437 4 ATCAACG 20675 0.0 27.616241 3 CAACGCA 20980 0.0 27.281515 5 TATCAAC 21895 0.0 26.353523 2 AACGCAG 21845 0.0 26.308588 6 ACCTGGG 6950 0.0 25.964516 3 GTACATG 18435 0.0 25.791435 1 TACATGG 18835 0.0 24.517994 2 ACATGGG 18565 0.0 24.224274 3 TATAACG 695 0.0 23.00558 2 ACGCAGA 24870 0.0 22.99505 7 TAACGCA 675 0.0 22.974012 4 CGCAGAG 25180 0.0 22.543991 8 CATGGGG 10740 0.0 21.877234 4 ATAACGC 795 0.0 20.68877 3 AGAGTAC 25820 0.0 19.50078 10-11 >>END_MODULE