FastQCFastQC Report
Mon 6 Jun 2016
ERR1211523_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211523_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4039329
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154660.3828853752690113No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT145350.3598369927282477No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT74040.18329777049604032No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88200.048.356631
GTATCAA143350.034.350291
TCAACGC166350.029.067554
CAACGCA167550.028.8004235
ATCAACG168250.028.7396553
AACGCAG173400.028.0132586
TATCAAC175400.027.692082
ACGCAGA191300.025.1965277
TATAACG3600.024.8162382
TACCTGG31850.024.6542552
CGCAGAG196200.024.4478368
GTACATG147450.024.3918611
TAACGCA4150.023.7786434
TACATGG149050.023.4706882
ACATGGG146200.022.7244453
GAGTACT125800.021.9636512-13
GTACCTG36950.021.9134311
GCAGAGT212300.021.8662559
CAGAGTA208050.020.46130410-11
CATGGGG77900.020.0270294
Produced by FastQC (version 0.11.5)