##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211523_2.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4039329
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.32927226279414	31.0	31.0	34.0	28.0	34.0
2	31.376990831893117	31.0	31.0	34.0	28.0	34.0
3	31.47112106986086	33.0	31.0	34.0	28.0	34.0
4	35.011273407043596	37.0	35.0	37.0	32.0	37.0
5	35.09697873087337	37.0	35.0	37.0	32.0	37.0
6	35.215753903680536	37.0	35.0	37.0	32.0	37.0
7	35.15266867343561	37.0	35.0	37.0	32.0	37.0
8	35.117147922340564	37.0	35.0	37.0	32.0	37.0
9	36.679524990413015	39.0	37.0	39.0	32.0	39.0
10-11	36.67715330937391	39.0	37.0	39.0	32.0	39.0
12-13	36.6256573802233	39.0	37.0	39.0	32.0	39.0
14-15	37.80357901027621	40.0	38.0	41.0	32.0	41.0
16-17	37.78010568091879	40.0	38.0	41.0	32.0	41.0
18-19	37.805583179780605	40.0	38.0	41.0	32.0	41.0
20-21	37.76603911194161	40.0	38.0	41.0	32.0	41.0
22-23	37.61727863712018	40.0	37.0	41.0	32.0	41.0
24-25	37.54058112127039	40.0	37.0	41.0	31.5	41.0
26-27	37.39300042160468	40.0	37.0	41.0	31.0	41.0
28-29	37.23426489894733	40.0	37.0	41.0	31.0	41.0
30-31	37.05632457767119	40.0	36.0	41.0	30.0	41.0
32-33	36.88782344790434	39.0	36.0	41.0	30.0	41.0
34-35	36.71151396679993	39.0	35.5	41.0	30.0	41.0
36-37	36.53724158146068	39.0	35.0	41.0	30.0	41.0
38-39	36.40731183322775	39.0	35.0	40.0	29.0	41.0
40-41	36.37394589546928	39.0	35.0	40.0	29.0	41.0
42-43	36.31328557292561	39.0	35.0	40.5	29.0	41.0
44-45	36.12866134449558	39.0	35.0	40.0	28.0	41.0
46-47	36.06851892975294	39.0	35.0	40.0	27.5	41.0
48-49	36.01185803384671	39.0	35.0	41.0	27.0	41.0
50-51	35.050165262596835	38.0	34.0	40.0	25.5	40.5
52-53	35.260669408211115	38.5	34.5	39.5	26.5	40.5
54-55	35.848975535293114	39.0	35.0	40.5	27.0	41.0
56-57	35.82144039269889	39.0	35.0	41.0	27.0	41.0
58-59	35.66888708495891	39.0	35.0	41.0	26.5	41.0
60-61	35.49019750557581	38.0	34.0	40.0	26.0	41.0
62-63	35.249061292110646	38.0	34.0	40.0	26.0	41.0
64-65	34.976241227193924	37.5	34.0	40.0	26.0	41.0
66-67	34.678480757571364	37.0	34.0	40.0	26.0	41.0
68-69	34.39749621781242	36.5	33.5	39.5	25.5	41.0
70-71	34.05195058387173	36.0	33.0	39.0	25.0	41.0
72-73	33.706197118382775	35.5	33.0	39.0	24.5	40.5
74-75	33.337130993786346	35.0	32.5	38.0	24.0	40.0
76-77	32.988252999446196	35.0	32.0	37.0	24.0	39.0
78-79	32.66381507918766	35.0	32.0	37.0	23.0	39.0
80-81	32.312856293706204	35.0	32.0	36.0	22.5	39.0
82-83	32.00479398929872	35.0	31.0	36.0	22.0	37.5
84-85	31.734630182389203	35.0	31.0	35.5	21.5	37.0
86-87	31.430847301618662	35.0	31.0	35.0	20.0	37.0
88-89	31.199706683956666	34.0	31.0	35.0	20.0	36.0
90-91	30.96528309528637	34.0	30.5	35.0	20.0	36.0
92-93	30.81029324424923	34.0	30.0	35.0	19.5	36.0
94-95	30.651028920892553	34.0	30.0	35.0	19.0	35.0
96-97	30.5509334594929	34.0	30.0	35.0	19.0	35.0
98-99	30.372604336017197	34.0	30.0	35.0	18.0	35.0
100	28.99737184071909	33.0	27.0	35.0	15.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3964227991570439
1101	2	0.3694457092284722
1101	3	0.337742249347869
1101	4	0.32020869268441743
1101	5	0.3002503942499217
1101	6	0.3187633396071874
1101	7	0.36817932805445963
1101	8	0.38798398470184736
1101	9	0.37613874056002317
1101	10-11	0.3954883753724303
1101	12-13	0.3568402955586336
1101	14-15	0.3578477532215061
1101	16-17	0.421347579247751
1101	18-19	0.40572973809703683
1101	20-21	0.45147991140195387
1101	22-23	0.430512738221033
1101	24-25	0.4242799265422903
1101	26-27	0.38195508987610793
1101	28-29	0.37419040802021897
1101	30-31	0.40844508739264285
1101	32-33	0.31141665773426297
1101	34-35	0.2769054886556006
1101	36-37	0.2540288355779339
1101	38-39	0.2278751373286383
1101	40-41	0.11057356927184969
1101	42-43	0.005921062712481273
1101	44-45	0.007456926730910141
1101	46-47	-0.070918568146638
1101	48-49	0.0693443340603821
1101	50-51	0.010197813107467368
1101	52-53	0.1148932699840799
1101	54-55	0.11500680676676467
1101	56-57	0.16953728270755875
1101	58-59	0.1622650963264647
1101	60-61	0.14831739215858875
1101	62-63	0.09830166606701596
1101	64-65	0.08048391709158409
1101	66-67	0.0969149217646077
1101	68-69	0.08801396982407539
1101	70-71	0.042827114688655854
1101	72-73	0.005794987378138217
1101	74-75	-0.04550812468446708
1101	76-77	-0.15842390494974268
1101	78-79	-0.23032927020377514
1101	80-81	-0.15910176402485376
1101	82-83	-0.11504365547654061
1101	84-85	-0.27715010915037297
1101	86-87	-0.3468035750176881
1101	88-89	-0.3146833856101736
1101	90-91	-0.33785129159361915
1101	92-93	-0.4126705172613967
1101	94-95	-0.5432571493182472
1101	96-97	-0.608235448893538
1101	98-99	-0.5967385246837935
1101	100	-0.42853651762015943
1102	1	0.38000283544827695
1102	2	0.27195447641391013
1102	3	0.2853632057811737
1102	4	0.2763140379381781
1102	5	0.270454984064628
1102	6	0.28459169126737294
1102	7	0.27436221765514546
1102	8	0.29382017469323785
1102	9	0.389747136051227
1102	10-11	0.3379901464604771
1102	12-13	0.33864785461251756
1102	14-15	0.36174569954386016
1102	16-17	0.3860778127393729
1102	18-19	0.4244932201029812
1102	20-21	0.328438568072805
1102	22-23	0.4267653697930953
1102	24-25	0.44345316673670254
1102	26-27	0.40177416977326175
1102	28-29	0.3310453740508521
1102	30-31	0.31893058778739203
1102	32-33	0.2081466713516491
1102	34-35	0.2658407100890088
1102	36-37	0.29588739281508225
1102	38-39	0.3476150439790615
1102	40-41	0.26872241562944055
1102	42-43	0.22330828237554812
1102	44-45	0.24035459565000394
1102	46-47	0.1949749449533087
1102	48-49	0.29144743655337635
1102	50-51	0.2516689700316377
1102	52-53	0.26313908654446294
1102	54-55	0.3389607262265528
1102	56-57	0.39837231754204083
1102	58-59	0.4288577288617077
1102	60-61	0.3588169261516043
1102	62-63	0.348929609663287
1102	64-65	0.37765737280196277
1102	66-67	0.32196178395880537
1102	68-69	0.32667974181252646
1102	70-71	0.34327011005996155
1102	72-73	0.3579381455522608
1102	74-75	0.32570204108849765
1102	76-77	0.3256600551922233
1102	78-79	0.29911663474214123
1102	80-81	0.16002117094967616
1102	82-83	0.21546368198159627
1102	84-85	0.19164725288181828
1102	86-87	0.17011900506371092
1102	88-89	0.07348449871810914
1102	90-91	-0.056122420930005745
1102	92-93	0.029466249523952825
1102	94-95	-0.07795813561435594
1102	96-97	-0.12954295641099023
1102	98-99	-0.11561180761093937
1102	100	-0.1515540920870322
1103	1	0.3667292428260396
1103	2	0.45419721485017206
1103	3	0.40331227478445797
1103	4	0.4134552601697621
1103	5	0.41714971754399954
1103	6	0.402777063876691
1103	7	0.44515640840415927
1103	8	0.40832474015788733
1103	9	0.4414710772706485
1103	10-11	0.42493352750212665
1103	12-13	0.4588238163265075
1103	14-15	0.5286916740011378
1103	16-17	0.47954301972114166
1103	18-19	0.4493335122154889
1103	20-21	0.5051484671095068
1103	22-23	0.568460427997941
1103	24-25	0.5440535578114805
1103	26-27	0.5325866460770783
1103	28-29	0.5399860317866896
1103	30-31	0.46857012056887015
1103	32-33	0.46922496617428777
1103	34-35	0.5413201409242347
1103	36-37	0.60402926976343
1103	38-39	0.5621474524591363
1103	40-41	0.5792520121624278
1103	42-43	0.46503206123224317
1103	44-45	0.471831311078148
1103	46-47	0.48966759229091394
1103	48-49	0.5356599728875224
1103	50-51	0.4573911156069457
1103	52-53	0.48996827831152956
1103	54-55	0.5643985351545737
1103	56-57	0.6254133487999383
1103	58-59	0.6196597261866685
1103	60-61	0.5006128028401804
1103	62-63	0.4271252593950763
1103	64-65	0.5145477007220336
1103	66-67	0.5505932021139515
1103	68-69	0.40831759128735
1103	70-71	0.5004158477309275
1103	72-73	0.4679738960718183
1103	74-75	0.3176880138256095
1103	76-77	0.3734052317024563
1103	78-79	0.3246135086012103
1103	80-81	0.4194752898729277
1103	82-83	0.47048003234809954
1103	84-85	0.37712495916148114
1103	86-87	0.3603945302413436
1103	88-89	0.37402044933877576
1103	90-91	0.30139341601919156
1103	92-93	0.245200581080951
1103	94-95	0.20747116901675966
1103	96-97	0.25955868617333167
1103	98-99	0.2825219204832585
1103	100	0.11971674300157531
1104	1	0.3899023604233314
1104	2	0.3656567499216976
1104	3	0.2830711580331915
1104	4	0.3636840711219165
1104	5	0.26912201026819815
1104	6	0.2607712666916413
1104	7	0.30511545316650057
1104	8	0.2876906550171441
1104	9	0.36518233323126736
1104	10-11	0.3527709515522659
1104	12-13	0.3657509957988978
1104	14-15	0.44267245580392967
1104	16-17	0.3908571444711839
1104	18-19	0.38181084743314386
1104	20-21	0.4488951022425667
1104	22-23	0.47326233347401825
1104	24-25	0.43630330784993987
1104	26-27	0.5347948400087787
1104	28-29	0.5040300250185012
1104	30-31	0.3935594250989425
1104	32-33	0.37740916379772926
1104	34-35	0.41564897014281144
1104	36-37	0.49956096681003714
1104	38-39	0.46599818215457134
1104	40-41	0.3801738655311411
1104	42-43	0.18166399766509045
1104	44-45	0.2893864227917291
1104	46-47	0.29937174838228486
1104	48-49	0.290366580524811
1104	50-51	0.2791577188218355
1104	52-53	0.4153072886560949
1104	54-55	0.497736915788316
1104	56-57	0.4191373912549423
1104	58-59	0.49707702256415587
1104	60-61	0.4615761402113492
1104	62-63	0.4630716522577174
1104	64-65	0.4077930908543195
1104	66-67	0.4337439914155894
1104	68-69	0.31761820369313654
1104	70-71	0.24371074947681137
1104	72-73	0.2596399618693894
1104	74-75	0.31304509831260674
1104	76-77	0.34188123539419735
1104	78-79	0.32238668772749435
1104	80-81	0.30312419965603965
1104	82-83	0.37680574185432647
1104	84-85	0.20989467728177047
1104	86-87	0.23811766898922926
1104	88-89	0.2836419258423888
1104	90-91	0.21968303011702162
1104	92-93	0.2713185581229425
1104	94-95	0.2808108475277571
1104	96-97	0.27227187452897184
1104	98-99	0.2210439988173576
1104	100	0.27474337351995715
1105	1	0.452983561495941
1105	2	0.47102858800417025
1105	3	0.44545918103053594
1105	4	0.3860938062378949
1105	5	0.29960933732673567
1105	6	0.29002284630985997
1105	7	0.3528575860806029
1105	8	0.3795061164495692
1105	9	0.382973146397255
1105	10-11	0.39166402637604847
1105	12-13	0.41717409096079194
1105	14-15	0.5109773921466996
1105	16-17	0.49883039125052164
1105	18-19	0.5255112431314117
1105	20-21	0.5770037694425554
1105	22-23	0.5576588506157449
1105	24-25	0.5456208732809529
1105	26-27	0.5209303228645155
1105	28-29	0.5268252012075436
1105	30-31	0.5697901448161744
1105	32-33	0.664383687098443
1105	34-35	0.6133897970169144
1105	36-37	0.6173405565265995
1105	38-39	0.5989467362833452
1105	40-41	0.6314814982625023
1105	42-43	0.6313515674134678
1105	44-45	0.6526509366655944
1105	46-47	0.6160731755336073
1105	48-49	0.6071459984387104
1105	50-51	0.6826922240800855
1105	52-53	0.7169652055626443
1105	54-55	0.7655737058095795
1105	56-57	0.6797789096082099
1105	58-59	0.7955406629414767
1105	60-61	0.7803380312808201
1105	62-63	0.8277958516498884
1105	64-65	0.7254515904883121
1105	66-67	0.6292804245163026
1105	68-69	0.5767669077085813
1105	70-71	0.6591965294619513
1105	72-73	0.6715505369920649
1105	74-75	0.657595280085765
1105	76-77	0.6312677385007675
1105	78-79	0.6221924169321014
1105	80-81	0.6237458704485874
1105	82-83	0.6068413808189987
1105	84-85	0.4816397123654035
1105	86-87	0.5008086083208063
1105	88-89	0.6422535925809036
1105	90-91	0.4374888786703188
1105	92-93	0.5280810314951268
1105	94-95	0.4833261000574929
1105	96-97	0.4659722596090212
1105	98-99	0.4466930953880812
1105	100	0.48611012632863293
1106	1	0.33323172289255254
1106	2	0.27941465639238316
1106	3	0.2483720236018776
1106	4	0.20401035095490982
1106	5	0.2536399392911264
1106	6	0.2637731120372351
1106	7	0.19746283153113353
1106	8	0.19913142019961327
1106	9	0.22980333904999384
1106	10-11	0.25594567156546333
1106	12-13	0.25257557535338293
1106	14-15	0.2596301164522288
1106	16-17	0.2972300510776549
1106	18-19	0.2674423065752407
1106	20-21	0.2554625098890426
1106	22-23	0.3455024806822493
1106	24-25	0.3565545260831584
1106	26-27	0.4365577380035006
1106	28-29	0.49224378312419503
1106	30-31	0.5356239829400238
1106	32-33	0.47273438616664976
1106	34-35	0.42416160838514116
1106	36-37	0.4004887198367655
1106	38-39	0.38765852238642395
1106	40-41	0.34824474173318976
1106	42-43	0.36574007761797844
1106	44-45	0.37622440807572843
1106	46-47	0.3749749249477432
1106	48-49	0.43792706804668313
1106	50-51	0.41340721344627696
1106	52-53	0.4824673197225806
1106	54-55	0.5369278608759984
1106	56-57	0.4768159225951507
1106	58-59	0.4419141757177698
1106	60-61	0.47529887302567886
1106	62-63	0.5758324230562337
1106	64-65	0.6473586676252197
1106	66-67	0.6166605570281192
1106	68-69	0.5855580266997578
1106	70-71	0.5681347513633668
1106	72-73	0.4294677488377161
1106	74-75	0.5313725812534571
1106	76-77	0.4879491886543903
1106	78-79	0.4487790197970085
1106	80-81	0.46074480377472327
1106	82-83	0.4733507964822863
1106	84-85	0.42162236806648323
1106	86-87	0.4914478419886734
1106	88-89	0.6104192178263084
1106	90-91	0.5623351280009707
1106	92-93	0.4570265510309639
1106	94-95	0.5238294088795996
1106	96-97	0.4714702674771658
1106	98-99	0.5181536564265308
1106	100	0.4442539736929483
1107	1	0.30880631741409914
1107	2	0.327215333232477
1107	3	0.3323097791179386
1107	4	0.28413091750117303
1107	5	0.2757546112432223
1107	6	0.31652630029671514
1107	7	0.26313786842128195
1107	8	0.3189635302817351
1107	9	0.32220543236535804
1107	10-11	0.36799142709592303
1107	12-13	0.35770502481907585
1107	14-15	0.4048864808741399
1107	16-17	0.4522952882715714
1107	18-19	0.42915207712891856
1107	20-21	0.43181770514806317
1107	22-23	0.46087543567289657
1107	24-25	0.47539107582414175
1107	26-27	0.4604045988212704
1107	28-29	0.45207905018570926
1107	30-31	0.5526752733631852
1107	32-33	0.47200167254711545
1107	34-35	0.4891471522069395
1107	36-37	0.6061547305219435
1107	38-39	0.5621501445680295
1107	40-41	0.694611733383951
1107	42-43	0.6639176423622857
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2313	1	-0.16168603921796176
2313	2	-0.2013859506102662
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2313	44-45	0.08218672934026472
2313	46-47	-9.591402601216714E-5
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2313	100	0.28508436711355856
2314	1	-0.346820568267578
2314	2	-0.25778709333228633
2314	3	-0.3359131469971288
2314	4	-0.28072959682518217
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2314	8	-0.22617216030590725
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2314	10-11	-0.3439543681729731
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2314	92-93	-1.1472061369742796
2314	94-95	-1.1498488867952794
2314	96-97	-1.0003144092496576
2314	98-99	-0.8052242240447534
2314	100	-0.38070155919899307
2315	1	-0.41308128453902526
2315	2	-0.35006139444219997
2315	3	-0.3638485998902645
2315	4	-0.3212725581799418
2315	5	-0.3470482699486581
2315	6	-0.3020221837939232
2315	7	-0.4227426304609452
2315	8	-0.34669800285394814
2315	9	-0.42241842369467975
2315	10-11	-0.5160936723006131
2315	12-13	-0.4637728873795268
2315	14-15	-0.40716031017581145
2315	16-17	-0.46431951490489354
2315	18-19	-0.3129229906595228
2315	20-21	-0.2937280228594332
2315	22-23	-0.2898489031948728
2315	24-25	-0.28507612195011944
2315	26-27	-0.3568543139036109
2315	28-29	-0.3765127510555786
2315	30-31	-0.248768933930414
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2315	100	-0.15148777097475374
2316	1	-0.458477707293131
2316	2	-0.8605185878396497
2316	3	-0.5608305580381483
2316	4	-0.4310714677839371
2316	5	-0.32585441081827327
2316	6	-0.3906000003044454
2316	7	-0.36939713262410123
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2316	9	-0.6288440827565864
2316	10-11	-0.5670717606320252
2316	12-13	-0.4893335828052656
2316	14-15	-0.6402493159546054
2316	16-17	-0.5484883473200526
2316	18-19	-0.43728947680392594
2316	20-21	-0.4517005710246451
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2316	30-31	-0.4404901982087992
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2316	38-39	-0.15342780503150522
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2316	54-55	-0.3300595640963664
2316	56-57	-0.3085541350270944
2316	58-59	-0.42326912340475076
2316	60-61	-0.4202713370134177
2316	62-63	-0.5423057075780235
2316	64-65	-0.252645385335164
2316	66-67	-0.16403774570695617
2316	68-69	-0.23396061564148596
2316	70-71	-0.30331005242229025
2316	72-73	-0.2616250250211323
2316	74-75	-0.041994219320464765
2316	76-77	-0.20567205804898947
2316	78-79	-0.46630037035691174
2316	80-81	-0.09981256763473567
2316	82-83	-0.05060068351843228
2316	84-85	0.00855956505184352
2316	86-87	0.0765111032750525
2316	88-89	-0.04246666548928246
2316	90-91	0.1407929414703304
2316	92-93	0.10260051085623445
2316	94-95	0.05123180912571357
2316	96-97	0.02194111296938317
2316	98-99	0.11988918512404467
2316	100	-0.2087381003760207
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
0	8.0
1	29.0
2	61.0
3	54.0
4	76.0
5	89.0
6	165.0
7	1175.0
8	1937.0
9	2402.0
10	3289.0
11	4909.0
12	6875.0
13	8687.0
14	10127.0
15	11616.0
16	13845.0
17	16901.0
18	20386.0
19	24319.0
20	28711.0
21	34658.0
22	39626.0
23	41865.0
24	41390.0
25	42273.0
26	45579.0
27	51113.0
28	58475.0
29	69268.0
30	82917.0
31	102015.0
32	127835.0
33	166365.0
34	229389.0
35	326708.0
36	500542.0
37	807033.0
38	890359.0
39	226014.0
40	244.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.946693619238225	19.230983819282212	13.522788222367643	23.29953433911192
2	15.613718791780817	20.8169121938345	40.46580152879849	23.1035674855862
3	18.126121503496936	26.534593197900204	30.763456927579412	24.575828371023444
4	12.370280025507519	17.43998613183425	39.071922536388456	31.117811306269772
5	12.806892336829259	37.56062070850948	35.604166584122126	14.028320370539127
6	29.391258579558787	37.57190687780807	18.957420829401087	14.079413713232054
7	25.75414683275042	33.11150541683471	23.59664752658159	17.53770022383328
8	24.807978165562634	35.310998127316715	21.60349620363227	18.27752750348838
9	25.9943824327854	15.835845228472078	21.44331122040348	36.726461118339046
10-11	23.778668037583692	26.43510861393827	29.66731453472028	20.11890881375776
12-13	24.679974137223496	24.641734563882554	29.221694081185	21.456597217708946
14-15	22.308734749948805	25.946684897438292	27.153503707850728	24.591076644762172
16-17	21.492565471781077	28.59816261086925	27.9457225961329	21.96354932121678
18-19	21.163406205206154	27.713567509466674	29.91729148493268	21.205734800394488
20-21	22.043648624573223	27.01879252845052	29.979659761253213	20.957899085723046
22-23	21.808397535231634	26.932499739625808	29.63660183751734	21.622500887625222
24-25	21.579893289166996	26.99401275889856	29.775868128913185	21.65022582302126
26-27	21.466700206786722	27.17595242716908	29.683031010085802	21.67431635595839
28-29	21.422552210657276	27.15589353423854	29.852503159779864	21.56905109532432
30-31	21.80945989259728	27.096847036731525	29.768346417290903	21.325346653380294
32-33	21.520809518285315	27.253952302345887	29.636965502418533	21.588272676950268
34-35	21.799780785406295	27.304077730677058	29.590466381338555	21.305675102578093
36-37	21.814732930762037	27.207127277463556	29.450930076874666	21.52720971489974
38-39	21.602187529442965	27.46959690887411	29.378609065158262	21.54960649652466
40-41	21.80654836451847	27.237443309650374	29.484986846489296	21.47102147934186
42-43	21.660115044065883	27.305865305771693	29.263464383120958	21.770555267041463
44-45	21.704341658356753	27.51143462239092	29.138539565582793	21.645684153669535
46-47	21.69288357424858	27.37903393547288	28.96353783891397	21.96454465136457
48-49	21.67392749570107	27.41693995362478	28.992731743007504	21.916400807666644
50-51	21.71815803440015	27.686851834130444	28.79791108777663	21.797079043692776
52-53	21.73496450310224	27.74501563284838	28.652851222801612	21.867168641247765
54-55	21.67924457283737	27.714856605431297	28.7229545714556	21.882944250275735
56-57	21.72675188223869	27.72397810893139	28.544373192107447	22.00489681672247
58-59	21.66645803842152	27.69099792027974	28.725397808251945	21.91714623304679
60-61	21.935659529199036	27.73721995103345	28.65156018658499	21.675560333182524
62-63	21.691860815055406	27.757863217187356	28.76959224937191	21.780683718385323
64-65	21.65624473367453	27.62339933113565	28.671990125899	22.048365809290825
66-67	21.64547234362772	27.682991315880408	28.550388037430572	22.1211483030613
68-69	21.623560861245263	27.64244664577051	28.650179627298893	22.083812865685335
70-71	21.615460983650895	27.512360928333674	28.65723050067967	22.21494758733576
72-73	21.588572182885592	27.726556021067793	28.429104844025467	22.255766952021144
74-75	21.610959292231776	27.785678303472316	28.372075629082456	22.231286775213448
76-77	21.538211822194743	27.60628853369856	28.510476659305304	22.345022984801393
78-79	21.523816645498215	27.544241118070694	28.44933641572754	22.48260582070355
80-81	21.543139716570217	27.70524887393243	28.332355220768672	22.419256188728674
82-83	21.690478923054847	27.701923811426887	28.344601550325404	22.26299571519286
84-85	21.580785956999105	27.751700046080703	28.263354303642853	22.404159693277343
86-87	21.435294309916657	27.701354010588723	28.421061363439954	22.44229031605466
88-89	21.50965256079997	27.53073689180993	28.410808570265157	22.548801977124945
90-91	21.71988208543556	27.542483268444336	28.35184580768852	22.38578883843159
92-93	21.583805673229527	27.48957525687622	28.40206684663005	22.52455222326421
94-95	21.401172804717763	27.49831374036653	28.394222740783515	22.706290714132194
96-97	21.44976346014118	27.443971814536113	28.38422595514427	22.722038770178436
98-99	21.423476820523828	27.318315939239586	28.327976876050727	22.930230364185856
100	21.486933972661774	27.289681664961847	28.33196699995294	22.89141736242344
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1359.0
1	1001.5
2	1309.5
3	2131.5
4	1695.5
5	1281.0
6	1886.0
7	3264.0
8	5362.0
9	6716.0
10	5588.5
11	4052.0
12	3929.5
13	4079.0
14	4298.0
15	4539.0
16	4768.5
17	5201.5
18	5699.0
19	6214.0
20	7039.5
21	8104.0
22	9688.0
23	11911.5
24	14775.0
25	18501.0
26	23259.5
27	29173.5
28	35990.5
29	43988.5
30	53087.5
31	61981.0
32	71707.5
33	82075.0
34	92451.5
35	102078.0
36	113403.0
37	124019.0
38	130802.0
39	136187.0
40	138794.5
41	140438.5
42	141656.0
43	143661.5
44	146569.0
45	149721.0
46	151509.5
47	151099.5
48	151826.5
49	151797.5
50	149615.0
51	142852.5
52	133462.5
53	126194.5
54	118294.0
55	108045.0
56	96819.5
57	85743.5
58	74746.5
59	63331.0
60	51494.0
61	41287.5
62	33268.5
63	25845.5
64	19079.0
65	13986.0
66	10310.0
67	7736.5
68	5863.0
69	4506.5
70	3514.5
71	2712.5
72	2144.0
73	1698.5
74	1317.0
75	964.5
76	744.5
77	570.0
78	422.0
79	308.5
80	248.0
81	220.5
82	205.0
83	171.0
84	119.5
85	103.5
86	88.5
87	68.0
88	51.5
89	33.0
90	24.0
91	25.5
92	20.0
93	12.5
94	10.5
95	6.5
96	7.5
97	8.5
98	8.0
99	8.5
100	12.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16255175055064838
2	0.10234373085232722
3	0.030277305958489643
4	0.032282589509297216
5	0.027356028686942806
6	0.001906257202619544
7	0.004010567101615145
8	0.005075100344636448
9	0.0052979096280595115
10-11	0.004542833723125797
12-13	0.007909729561518756
14-15	0.0182208480665972
16-17	0.00834296983484138
18-19	0.017032531888340863
20-21	0.019372029364283026
22-23	0.046307196071426714
24-25	0.016277455983407147
26-27	0.009531286013097719
28-29	0.02656381790143858
30-31	0.01496535686991577
32-33	0.020510832368445354
34-35	0.020312779672069296
36-37	0.021600122198513663
38-39	0.019817647931129154
40-41	0.026081064454021943
42-43	0.034609708691715875
44-45	0.03670164029718797
46-47	0.020820289706532942
48-49	0.013220017483101773
50-51	0.011895540076086895
52-53	0.021723905133748698
54-55	0.02552404124546428
56-57	0.01948343400599456
58-59	0.025883011757645888
60-61	0.026291495443921505
62-63	0.051543214231868716
64-65	0.031948375584162615
66-67	0.021426826089184614
68-69	0.02739316356751332
70-71	0.042271872382764565
72-73	0.03967243074282882
74-75	0.04017994077729247
76-77	0.022268550048782854
78-79	0.0171686931170994
80-81	0.023246435237139634
82-83	0.01875311468810785
84-85	0.018864519329819383
86-87	0.02183530977546023
88-89	0.01825798294716771
90-91	0.02849483169110513
92-93	0.027232245751707768
94-95	0.019929052572840687
96-97	0.0143711987807876
98-99	0.01845603564354377
100	0.04354683661568543
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4039329.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	67.0102641522228
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.34302894051524	51.82776799636939
2	15.246255323285732	20.433111930912197
3	4.069476856844385	8.180901574154987
4	1.4058166959800864	3.7681659258892273
5	0.6458677681256229	2.1639884874752284
6	0.354900540067101	1.4269187362597782
7	0.2125494823758395	0.9970097871596263
8	0.14019709283964363	0.7515715379646578
9	0.09666949074572957	0.5830063299298976
>10	0.4450276793361966	5.149432001926611
>50	0.024239110576089646	1.109297252226553
>100	0.014821432380332124	1.7454618788950678
>500	7.516529932980828E-4	0.3588012457651163
>1k	2.6528928992447217E-4	0.39631093626349334
>5k	4.421488165407869E-5	0.21936948056929612
>10k+	8.842976330815738E-5	0.8888848982387159
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15466	0.3828853752690113	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	14535	0.3598369927282477	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7404	0.18329777049604032	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	4.951317409401413E-5	0.0
10-11	0.0	0.0	0.0	4.951317409401413E-5	0.0
12-13	0.0	2.4756587047007066E-5	0.0	4.951317409401413E-5	0.0
14-15	0.0	4.951317409401413E-5	0.0	4.951317409401413E-5	0.0
16-17	0.0	4.951317409401413E-5	0.0	9.902634818802827E-5	0.0
18-19	0.0	4.951317409401413E-5	0.0	1.7329610932904945E-4	0.0
20-21	0.0	6.189146761751766E-5	0.0	1.7329610932904945E-4	0.0
22-23	0.0	7.42697611410212E-5	0.0	2.2280928342306358E-4	0.0
24-25	0.0	7.42697611410212E-5	0.0	2.847007510405812E-4	0.0
26-27	0.0	7.42697611410212E-5	0.0	5.941580891281696E-4	0.0
28-29	0.0	7.42697611410212E-5	0.0	9.655068948332755E-4	0.0
30-31	0.0	7.42697611410212E-5	0.0	0.0034164090124869746	0.0
32-33	0.0	7.42697611410212E-5	0.0	0.009395124784339182	2.4756587047007066E-5
34-35	0.0	7.42697611410212E-5	0.0	0.02043656260730433	2.4756587047007066E-5
36-37	0.0	7.42697611410212E-5	0.0	0.037753795246685776	2.4756587047007066E-5
38-39	0.0	7.42697611410212E-5	0.0	0.06768450898651732	2.4756587047007066E-5
40-41	0.0	7.42697611410212E-5	0.0	0.10026417754037861	2.4756587047007066E-5
42-43	0.0	7.42697611410212E-5	0.0	0.1349233994061885	2.4756587047007066E-5
44-45	0.0	8.664805466452474E-5	0.0	0.17912628557861962	2.4756587047007066E-5
46-47	1.2378293523503533E-5	9.902634818802827E-5	0.0	0.234741958379721	2.4756587047007066E-5
48-49	2.4756587047007066E-5	9.902634818802827E-5	0.0	0.294380576575961	2.4756587047007066E-5
50-51	2.4756587047007066E-5	9.902634818802827E-5	0.0	0.35529415900512185	2.4756587047007066E-5
52-53	2.4756587047007066E-5	9.902634818802827E-5	0.0	0.4196984202079108	2.4756587047007066E-5
54-55	2.4756587047007066E-5	9.902634818802827E-5	0.0	0.4957754122033635	2.4756587047007066E-5
56-57	2.4756587047007066E-5	9.902634818802827E-5	0.0	0.5893057980669563	2.4756587047007066E-5
58-59	2.4756587047007066E-5	9.902634818802827E-5	0.0	0.6964027936323087	2.4756587047007066E-5
60-61	2.4756587047007066E-5	9.902634818802827E-5	0.0	0.811038169953475	2.4756587047007066E-5
62-63	2.4756587047007066E-5	9.902634818802827E-5	0.0	0.9274931554225962	2.4756587047007066E-5
64-65	2.4756587047007066E-5	9.902634818802827E-5	0.0	1.0520064099755182	2.4756587047007066E-5
66-67	2.4756587047007066E-5	9.902634818802827E-5	0.0	1.2013628996301118	2.4756587047007066E-5
68-69	2.4756587047007066E-5	9.902634818802827E-5	0.0	1.3736935020643282	2.4756587047007066E-5
70-71	2.4756587047007066E-5	9.902634818802827E-5	0.0	1.5658912656037673	2.4756587047007066E-5
72-73	2.4756587047007066E-5	9.902634818802827E-5	0.0	1.7620129481901574	2.4756587047007066E-5
74-75	2.4756587047007066E-5	9.902634818802827E-5	0.0	1.9615015266149403	2.4756587047007066E-5
76-77	2.4756587047007066E-5	9.902634818802827E-5	0.0	2.1869350082649865	2.4756587047007066E-5
78-79	2.4756587047007066E-5	9.902634818802827E-5	0.0	2.4461612312341976	2.4756587047007066E-5
80-81	2.4756587047007066E-5	9.902634818802827E-5	0.0	2.720204271551042	4.951317409401413E-5
82-83	2.4756587047007066E-5	9.902634818802827E-5	0.0	3.0081728920818285	7.42697611410212E-5
84-85	2.4756587047007066E-5	9.902634818802827E-5	0.0	3.303060978692253	7.42697611410212E-5
86-87	2.4756587047007066E-5	9.902634818802827E-5	0.0	3.624698557606969	7.42697611410212E-5
88	2.4756587047007066E-5	9.902634818802827E-5	0.0	3.8808673420758746	7.42697611410212E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	8820	0.0	48.35663	1
GTATCAA	14335	0.0	34.35029	1
TCAACGC	16635	0.0	29.06755	4
CAACGCA	16755	0.0	28.800423	5
ATCAACG	16825	0.0	28.739655	3
AACGCAG	17340	0.0	28.013258	6
TATCAAC	17540	0.0	27.69208	2
ACGCAGA	19130	0.0	25.196527	7
TATAACG	360	0.0	24.816238	2
TACCTGG	3185	0.0	24.654255	2
CGCAGAG	19620	0.0	24.447836	8
GTACATG	14745	0.0	24.391861	1
TAACGCA	415	0.0	23.778643	4
TACATGG	14905	0.0	23.470688	2
ACATGGG	14620	0.0	22.724445	3
GAGTACT	12580	0.0	21.96365	12-13
GTACCTG	3695	0.0	21.913431	1
GCAGAGT	21230	0.0	21.866255	9
CAGAGTA	20805	0.0	20.461304	10-11
CATGGGG	7790	0.0	20.027029	4
>>END_MODULE