FastQCFastQC Report
Mon 6 Jun 2016
ERR1211523_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211523_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4039329
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160960.3984820251086257No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT154760.3831329411394813No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84540.20929218689539772No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA96550.042.224571
GTATCAA148950.032.3264431
TCAACGC180300.026.4284554
ATCAACG180450.026.3550553
CAACGCA181850.026.1250215
TATCAAC186650.025.5441972
AACGCAG187950.025.3021246
GTACATG159450.022.5303151
ACGCAGA211800.022.2751797
CGCAGAG214850.021.9589618
TACCTGG31950.021.9279352
TACATGG161750.021.5114542
GTACCTG34300.021.248881
ACATGGG158000.021.1471983
CATGGGG77550.020.602824
GAGTACT136400.019.964612-13
GCAGAGT236500.019.5511389
ATGGGGG45950.019.0215645
AGTACTT146600.018.14278412-13
AGAGTAC218000.018.09579510-11
Produced by FastQC (version 0.11.5)