##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1211523_1.fastq.gz
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	4039329
Sequences flagged as poor quality	0
Sequence length	100
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.122049974141746	33.0	31.0	34.0	30.0	34.0
2	32.340749168983265	34.0	31.0	34.0	31.0	34.0
3	32.34893146856817	34.0	31.0	34.0	30.0	34.0
4	35.987313486967764	37.0	35.0	37.0	35.0	37.0
5	35.90621140293351	37.0	35.0	37.0	35.0	37.0
6	35.925665129035046	37.0	35.0	37.0	35.0	37.0
7	35.855181145185256	37.0	35.0	37.0	35.0	37.0
8	35.85245049363397	37.0	35.0	37.0	35.0	37.0
9	37.50021278286567	39.0	37.0	39.0	35.0	39.0
10-11	37.46908781136669	39.0	37.0	39.0	34.5	39.0
12-13	37.38570675971182	39.0	37.0	39.0	34.0	39.0
14-15	38.61209782614885	40.0	38.0	41.0	34.0	41.0
16-17	38.536086934240814	40.0	38.0	41.0	34.0	41.0
18-19	38.537345063994536	40.0	38.0	41.0	34.0	41.0
20-21	38.486145966322624	40.0	38.0	41.0	34.0	41.0
22-23	38.37645955553509	40.0	38.0	41.0	34.0	41.0
24-25	38.270345396475506	40.0	38.0	41.0	34.0	41.0
26-27	38.0943162837194	40.0	37.5	41.0	33.0	41.0
28-29	38.12834446018138	40.0	38.0	41.0	33.0	41.0
30-31	38.049356712463876	40.0	37.5	41.0	33.0	41.0
32-33	37.85549654410423	40.0	37.0	41.0	33.0	41.0
34-35	37.735065279406555	40.0	37.0	41.0	33.0	41.0
36-37	37.672096281337815	40.0	37.0	41.0	33.0	41.0
38-39	37.57732249588979	40.0	37.0	41.0	32.5	41.0
40-41	37.41479983432892	40.0	36.5	41.0	31.5	41.0
42-43	37.26237253761701	40.0	36.0	41.0	31.0	41.0
44-45	37.0750383293859	39.5	36.0	41.0	31.0	41.0
46-47	36.8145741532814	39.0	36.0	41.0	30.0	41.0
48-49	36.587647230517746	39.0	35.0	41.0	30.0	41.0
50-51	36.39032510102544	39.0	35.0	41.0	29.0	41.0
52-53	36.196199789618525	39.0	35.0	40.0	28.5	41.0
54-55	36.00337890278311	39.0	35.0	40.0	28.0	41.0
56-57	35.80779802784076	38.0	35.0	40.0	28.0	41.0
58-59	35.55683035474456	38.0	34.0	40.0	27.0	41.0
60-61	35.2459710511325	38.0	34.0	40.0	26.0	41.0
62-63	34.930496995911945	37.5	34.0	40.0	26.0	41.0
64-65	34.6226593575319	37.0	33.0	40.0	26.0	41.0
66-67	34.28621288337742	36.5	33.0	39.0	25.5	41.0
68-69	33.89099130078288	36.0	32.5	39.0	25.0	40.0
70-71	33.50322664977277	35.5	32.0	39.0	24.0	40.0
72-73	33.094411472796594	35.0	31.5	38.0	23.5	40.0
74-75	32.684435088104976	35.0	31.0	37.0	22.5	39.0
76-77	31.131411306184766	33.5	29.5	35.5	21.0	38.5
78-79	31.956258824175997	34.0	31.0	36.0	22.0	39.0
80-81	31.915463187078842	34.5	31.0	36.0	22.0	37.5
82-83	31.6222423328231	34.0	31.0	35.5	21.5	37.0
84-85	31.317025674313726	34.0	30.5	35.0	20.0	37.0
86-87	31.016074080620818	34.0	30.0	35.0	20.0	36.0
88-89	30.728058298791705	34.0	30.0	35.0	20.0	36.0
90-91	30.43916093489785	34.0	29.0	35.0	19.0	35.5
92-93	30.22353762221399	34.0	29.0	35.0	18.5	35.0
94-95	30.00373527385365	34.0	29.0	35.0	18.0	35.0
96-97	29.79637645262369	34.0	29.0	35.0	18.0	35.0
98-99	29.6293394274148	34.0	29.0	35.0	17.0	35.0
100	27.76580565732576	31.0	25.0	34.0	10.0	35.0
>>END_MODULE
>>Per tile sequence quality	pass
#Tile	Base	Mean
1101	1	0.3329667384545587
1101	2	0.24380986226007195
1101	3	0.26654523792414153
1101	4	0.17981781829084298
1101	5	0.16711536217226097
1101	6	0.1730560904278633
1101	7	0.22687004228141916
1101	8	0.20846252929679565
1101	9	0.1683691586627134
1101	10-11	0.2618655493417634
1101	12-13	0.23008507161951286
1101	14-15	0.3001496846415179
1101	16-17	0.3032425387139739
1101	18-19	0.3494184673710876
1101	20-21	0.37421914537060275
1101	22-23	0.33056945478210764
1101	24-25	0.2985011557770676
1101	26-27	0.25783047246132895
1101	28-29	0.24170006054061588
1101	30-31	0.317222307748402
1101	32-33	0.3089719848658845
1101	34-35	0.2882436332009277
1101	36-37	0.35657690201071546
1101	38-39	0.2952639314805552
1101	40-41	0.3510436888822426
1101	42-43	0.3585145380689525
1101	44-45	0.34969527891979624
1101	46-47	0.3312079791849811
1101	48-49	0.47349293517917346
1101	50-51	0.46565360570459546
1101	52-53	0.32011369567256054
1101	54-55	0.3543969617297904
1101	56-57	0.42372212245469143
1101	58-59	0.39877295916445377
1101	60-61	0.35625034670987077
1101	62-63	0.33420406837627326
1101	64-65	0.25078232231668096
1101	66-67	0.35082109890777957
1101	68-69	0.3249282466674188
1101	70-71	0.30896573423524387
1101	72-73	0.12268134156671096
1101	74-75	0.1882875256464871
1101	76-77	0.17437595563925257
1101	78-79	0.23127463587850983
1101	80-81	0.15891467346474286
1101	82-83	0.25338017203780083
1101	84-85	0.30068607897488775
1101	86-87	0.18423009186274086
1101	88-89	0.17386613046138066
1101	90-91	0.07602667801145202
1101	92-93	0.055002978894744814
1101	94-95	0.04262231497858693
1101	96-97	0.08677067415662165
1101	98-99	-0.0331024447590309
1101	100	0.11724383058095711
1102	1	0.3538562070889171
1102	2	0.25610963560874467
1102	3	0.3558299210143332
1102	4	0.20818284743561577
1102	5	0.20611765178432506
1102	6	0.19422470563640815
1102	7	0.17974493602158503
1102	8	0.2380922711112703
1102	9	0.28905646939163887
1102	10-11	0.21593950945540286
1102	12-13	0.2732519513687919
1102	14-15	0.331262869316042
1102	16-17	0.3839685791233194
1102	18-19	0.347567269056519
1102	20-21	0.3305639497904025
1102	22-23	0.39052759478119725
1102	24-25	0.29784823390128423
1102	26-27	0.423087832092115
1102	28-29	0.44569701564203257
1102	30-31	0.43259210504789536
1102	32-33	0.4877918148196372
1102	34-35	0.514826918357322
1102	36-37	0.5400638839960621
1102	38-39	0.5844598095686919
1102	40-41	0.5398618038605605
1102	42-43	0.4558982852881144
1102	44-45	0.5764096932338987
1102	46-47	0.49968985219965845
1102	48-49	0.5455873865254048
1102	50-51	0.5989626562641135
1102	52-53	0.6513042993940417
1102	54-55	0.695134007836046
1102	56-57	0.5892461531394702
1102	58-59	0.5984244835640595
1102	60-61	0.5234252046905112
1102	62-63	0.4972258311313169
1102	64-65	0.5692748621735149
1102	66-67	0.590594086933244
1102	68-69	0.591421919454028
1102	70-71	0.46642172615677424
1102	72-73	0.5114573121676287
1102	74-75	0.41173921008141434
1102	76-77	0.49559638511529513
1102	78-79	0.6062062929086984
1102	80-81	0.5410922106859175
1102	82-83	0.5084540859538755
1102	84-85	0.5761041614405791
1102	86-87	0.4185591769091772
1102	88-89	0.39880929329884296
1102	90-91	0.506024464015205
1102	92-93	0.5042877556875318
1102	94-95	0.49371476196089503
1102	96-97	0.478308392611698
1102	98-99	0.4430673504484979
1102	100	0.4672864979495941
1103	1	0.30509648287738855
1103	2	0.24522847986689555
1103	3	0.29869249894844785
1103	4	0.17315791378493373
1103	5	0.17709355715405906
1103	6	0.20124140881274855
1103	7	0.2262739278630761
1103	8	0.21790751109217865
1103	9	0.3230532519765248
1103	10-11	0.24831782077761488
1103	12-13	0.263420887603246
1103	14-15	0.3834444979283944
1103	16-17	0.4127847836266483
1103	18-19	0.37842957316807
1103	20-21	0.4199219280320321
1103	22-23	0.3798321688466828
1103	24-25	0.36360240004136557
1103	26-27	0.4081007640647627
1103	28-29	0.37619578080758487
1103	30-31	0.45894028623417427
1103	32-33	0.4303501820297413
1103	34-35	0.42945834616646295
1103	36-37	0.5554932807789967
1103	38-39	0.5014049063895314
1103	40-41	0.5766884153765517
1103	42-43	0.5617130434466162
1103	44-45	0.49711447477264414
1103	46-47	0.601519482784937
1103	48-49	0.5592025991240561
1103	50-51	0.5324600856670401
1103	52-53	0.6251557804932588
1103	54-55	0.5892623130148067
1103	56-57	0.5789588281401663
1103	58-59	0.6227926072827188
1103	60-61	0.6445978322637416
1103	62-63	0.6417439165850212
1103	64-65	0.6627224067223381
1103	66-67	0.5637109863490934
1103	68-69	0.5640139922908318
1103	70-71	0.6480848954423024
1103	72-73	0.55994551761178
1103	74-75	0.4915297445971021
1103	76-77	0.46736073710367165
1103	78-79	0.49813052392367396
1103	80-81	0.5171891245652134
1103	82-83	0.48908892864632136
1103	84-85	0.4244782083274288
1103	86-87	0.40424810833417624
1103	88-89	0.39264323922239797
1103	90-91	0.4411521030679175
1103	92-93	0.3650818937783562
1103	94-95	0.4810052701474845
1103	96-97	0.5614733294569767
1103	98-99	0.49298500621140917
1103	100	0.5625460325916585
1104	1	0.28680122061004454
1104	2	0.279697936488958
1104	3	0.29361275160188427
1104	4	0.07515736579231458
1104	5	0.17357606945985538
1104	6	0.18885389150771914
1104	7	0.1814310671493402
1104	8	0.2289298538086797
1104	9	0.27262711561289166
1104	10-11	0.2952246668397578
1104	12-13	0.28967285845388346
1104	14-15	0.3812381066039805
1104	16-17	0.3410572369030689
1104	18-19	0.29465520191748595
1104	20-21	0.2697052546356318
1104	22-23	0.2995362287990062
1104	24-25	0.3689783758527838
1104	26-27	0.41424344884402586
1104	28-29	0.44379175161977
1104	30-31	0.4398218333038528
1104	32-33	0.420155236059891
1104	34-35	0.4400961326608481
1104	36-37	0.4007580717726853
1104	38-39	0.4572398260172861
1104	40-41	0.5258723149689217
1104	42-43	0.5513510558677837
1104	44-45	0.5805277236958801
1104	46-47	0.48430013672279415
1104	48-49	0.5762168084764596
1104	50-51	0.43993525775594833
1104	52-53	0.5445275598349042
1104	54-55	0.5268404259974204
1104	56-57	0.4565395609120486
1104	58-59	0.5043258569750719
1104	60-61	0.43358702623390144
1104	62-63	0.376870603825445
1104	64-65	0.546484880764794
1104	66-67	0.5499522857645687
1104	68-69	0.5531298494666501
1104	70-71	0.4806389015567518
1104	72-73	0.572780682394118
1104	74-75	0.6054461132326949
1104	76-77	0.49476577575157066
1104	78-79	0.607596255157322
1104	80-81	0.724172905906542
1104	82-83	0.5395589284540527
1104	84-85	0.6614165158954961
1104	86-87	0.7128502705015372
1104	88-89	0.7143497555275822
1104	90-91	0.6329465145046207
1104	92-93	0.528304378780664
1104	94-95	0.608949528723091
1104	96-97	0.6595141356790997
1104	98-99	0.697395265210023
1104	100	0.7742105841991034
1105	1	0.2773811682028011
1105	2	0.20527043328278438
1105	3	0.2055579420936553
1105	4	0.18519909323939032
1105	5	0.11997130349691787
1105	6	0.15699617808856914
1105	7	0.2252686713750478
1105	8	0.1898065230711481
1105	9	0.20859749589988752
1105	10-11	0.2781506891239829
1105	12-13	0.23132382255562334
1105	14-15	0.34709838028625484
1105	16-17	0.38765865536869626
1105	18-19	0.4086882619309833
1105	20-21	0.29832680268166456
1105	22-23	0.3282647553246747
1105	24-25	0.39954252567064685
1105	26-27	0.38672046094730206
1105	28-29	0.40736390693192703
1105	30-31	0.44295935549326515
1105	32-33	0.42020593801922956
1105	34-35	0.5423682493864206
1105	36-37	0.5717591631124535
1105	38-39	0.5896862980209931
1105	40-41	0.5551515550277841
1105	42-43	0.5081656221849897
1105	44-45	0.5075139822438075
1105	46-47	0.5543381775268799
1105	48-49	0.662418156097381
1105	50-51	0.6601588730403094
1105	52-53	0.6864053926409639
1105	54-55	0.6070896821386214
1105	56-57	0.6107792215879897
1105	58-59	0.600793156812756
1105	60-61	0.670664556803203
1105	62-63	0.6033695820349649
1105	64-65	0.6002336827651007
1105	66-67	0.6662799412571232
1105	68-69	0.652950592822144
1105	70-71	0.7079147612018346
1105	72-73	0.7512469603351448
1105	74-75	0.7738061368560949
1105	76-77	0.702338269997032
1105	78-79	0.802104473759119
1105	80-81	0.7483217447732784
1105	82-83	0.7476044109254012
1105	84-85	0.7560948594800045
1105	86-87	0.8024591614272332
1105	88-89	0.764146140706174
1105	90-91	0.8189099398631612
1105	92-93	0.8330912702164603
1105	94-95	0.8916007410786122
1105	96-97	0.9007066478863237
1105	98-99	0.913023318609433
1105	100	1.0502781338620721
1106	1	0.21645831180056518
1106	2	0.13276259530553602
1106	3	0.19433653516955474
1106	4	0.08237485957204882
1106	5	0.1137271153846271
1106	6	0.09407632752957085
1106	7	0.11174465636486275
1106	8	0.10833207885570317
1106	9	0.0638625786274929
1106	10-11	0.14630709168195466
1106	12-13	0.16069291126522245
1106	14-15	0.16997222406501322
1106	16-17	0.19644037391088887
1106	18-19	0.20914195645905664
1106	20-21	0.2047499339346217
1106	22-23	0.22319429535202318
1106	24-25	0.21235166738247102
1106	26-27	0.21823309775442112
1106	28-29	0.272000630815846
1106	30-31	0.35183217160113855
1106	32-33	0.36134783429368866
1106	34-35	0.32652913069890843
1106	36-37	0.3085903784169304
1106	38-39	0.3230284462358526
1106	40-41	0.3925330678057506
1106	42-43	0.32956773104344705
1106	44-45	0.31146411573750044
1106	46-47	0.385758456752626
1106	48-49	0.3909327973228116
1106	50-51	0.3325004464579777
1106	52-53	0.41144740972008265
1106	54-55	0.2779602264538994
1106	56-57	0.36264637138962996
1106	58-59	0.4467923665058251
1106	60-61	0.4659510629344581
1106	62-63	0.4788251874996874
1106	64-65	0.4597656439256639
1106	66-67	0.4834904850683941
1106	68-69	0.4282908676394115
1106	70-71	0.5474066695484012
1106	72-73	0.49188368442820973
1106	74-75	0.49684216713536955
1106	76-77	0.4507393048620294
1106	78-79	0.5580107842209152
1106	80-81	0.5814441169739624
1106	82-83	0.5990359351376178
1106	84-85	0.49705291371463645
1106	86-87	0.4654537711742357
1106	88-89	0.5479351344011825
1106	90-91	0.4172039204492961
1106	92-93	0.528492908320505
1106	94-95	0.631528588711447
1106	96-97	0.4727463194540853
1106	98-99	0.5873825378932587
1106	100	0.734747985660178
1107	1	0.3190040805634169
1107	2	0.24849767900855113
1107	3	0.3114924161946462
1107	4	0.1833123853614822
1107	5	0.19573779806419367
1107	6	0.1982034047194361
1107	7	0.1961880610105382
1107	8	0.22074450438833537
1107	9	0.27086239744568985
1107	10-11	0.23628319666780584
1107	12-13	0.29109690910357244
1107	14-15	0.31443448915701566
1107	16-17	0.3226612174601655
1107	18-19	0.31643454753266553
1107	20-21	0.34047820257102046
1107	22-23	0.3853626578772449
1107	24-25	0.33770308882966305
1107	26-27	0.41408679055119535
1107	28-29	0.4278737478052719
1107	30-31	0.44331708097343636
1107	32-33	0.3985166470329773
1107	34-35	0.40616393583294297
1107	36-37	0.5495490227698951
1107	38-39	0.4908686448248929
1107	40-41	0.48123267886776944
1107	42-43	0.5310586521438552
1107	44-45	0.5983595506607386
1107	46-47	0.5663761222759831
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2316	68-69	-0.04652875966694836
2316	70-71	-0.2989127318133953
2316	72-73	-0.43500442067558964
2316	74-75	-0.48219716636646837
2316	76-77	-0.3995035695868303
2316	78-79	-0.27514072437391235
2316	80-81	-0.2308823556867452
2316	82-83	-0.03289486646773554
2316	84-85	-0.11759544250675447
2316	86-87	-0.10041624942884297
2316	88-89	-0.09071297034518722
2316	90-91	0.10091200900935604
2316	92-93	0.08693348553865476
2316	94-95	-0.036609430227045436
2316	96-97	0.023574591373900944
2316	98-99	-0.04205952264456769
2316	100	-0.21687386866813085
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	8.0
11	14.0
12	47.0
13	133.0
14	392.0
15	1114.0
16	2502.0
17	5218.0
18	9439.0
19	14520.0
20	20086.0
21	26951.0
22	34540.0
23	38882.0
24	40604.0
25	44423.0
26	51781.0
27	61774.0
28	74957.0
29	90293.0
30	109257.0
31	130914.0
32	159649.0
33	201255.0
34	262734.0
35	356460.0
36	518152.0
37	809182.0
38	840439.0
39	133590.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.435733401993964	20.74893728693129	14.537840507626528	23.277488803448215
2	14.939968562924316	21.881269919128187	39.80404470555302	23.37471681239448
3	17.17351591277798	27.36428901931299	31.258675696611864	24.203519371297162
4	12.060540117668756	18.450401739903274	38.80684919806749	30.68220894436048
5	12.491632138924318	38.14172581935719	35.56789058600775	13.798751455710741
6	29.351805203630832	38.37795952016379	18.56521992080301	13.705015355402367
7	25.6637179095835	33.51267004990186	23.35798841837345	17.4656236221412
8	24.99429972245033	35.92538961799352	21.21627211998583	17.864038539570323
9	25.90321954542934	15.857612949480707	21.27908404566304	36.96008345942691
10-11	23.835481091042595	26.89490011831173	29.44814596681776	19.821472823827918
12-13	24.695116441369347	24.953711371368858	29.111456382978464	21.239715804283335
14-15	22.26276938570738	26.237488949278458	27.066067161154738	24.43367450385943
16-17	21.469896755383154	28.955221220162304	27.81409259868781	21.760789425766728
18-19	21.208646626081116	27.965446372769055	29.86888897333725	20.957018027812584
20-21	22.130071101977286	27.19905941735718	29.890707277693828	20.780162202971702
22-23	21.895072176591707	27.16203607084246	29.575295797891183	21.367595954674652
24-25	21.581832423611054	27.307141187841143	29.71198427065096	21.399042117896837
26-27	21.572557934918205	27.33063535246181	29.649133752067492	21.447672960552488
28-29	21.541415033661924	27.343151579909495	29.82064634259257	21.29478704383601
30-31	21.882786480914476	27.264178211191304	29.751853839293286	21.101181468600934
32-33	21.658208147287787	27.429382122272823	29.596408614877888	21.3160011155615
34-35	21.87741851148185	27.513308466764606	29.549413516381385	21.05985950537216
36-37	21.940538113694373	27.42194053811369	29.406553376576227	21.23096797161571
38-39	21.780754938392334	27.607661780470234	29.35727247745261	21.254310803684824
40-41	21.9404392762139	27.381461538109214	29.472349371020407	21.20574981465648
42-43	21.775170196660003	27.540645110176765	29.225621444500632	21.458563248662596
44-45	21.81078282777017	27.6834518160612	29.128331228092414	21.377434128076224
46-47	21.817187663829664	27.53204918315883	28.943514285668304	21.7072488673432
48-49	21.772548099170116	27.63500312825034	28.937417414562372	21.655031358017172
50-51	21.844131613016316	27.845781021796608	28.707722139735274	21.6023652254518
52-53	21.866585131713403	27.913487123274784	28.567173804998188	21.652753940013632
54-55	21.772732876844593	27.89133742552432	28.670939914904743	21.664989782726344
56-57	21.858214092385474	27.90798316149837	28.431862235932876	21.80194051018328
58-59	21.79222083332405	27.830882707920658	28.613530542019365	21.76336591673593
60-61	22.01635761805015	27.956996169011138	28.553611375785138	21.473034837153573
62-63	21.816789188756896	27.909063481921777	28.673449794623174	21.600697534698156
64-65	21.77981020174003	27.7565255453237	28.583388202095794	21.88027605084047
66-67	21.757289491878762	27.833055517557483	28.463764384161962	21.94589060640179
68-69	21.711085600703857	27.831231200672363	28.523094738398036	21.934588460225736
70-71	21.719364983477167	27.73868979718911	28.517834694917134	22.024110524416596
72-73	21.645221265219057	27.949071261833367	28.28192135512869	22.12378611781888
74-75	21.655699385882617	28.01496034560211	28.18973455586567	22.139605712649608
76-77	21.563994917319796	27.8596482106948	28.379168438363216	22.197188433622188
78-79	21.557551891100086	27.81819264441312	28.352556064769807	22.271699399716987
80-81	21.546405292999964	27.9747035858869	28.17500957212804	22.303881548985093
82-83	21.666430822603047	27.982867197755308	28.1976771897688	22.153024789872845
84-85	21.513186255660017	28.04553863097872	28.13209010861833	22.309185004742936
86-87	21.448819599550323	27.971974465514084	28.223481227992846	22.355724706942745
88-89	21.449576341379288	27.879173784595363	28.20626215949393	22.46498771453142
90-91	21.640120052510902	27.906736319673175	28.14192850410169	22.311215123714234
92-93	21.512345656081916	27.866997327128253	28.134556915724424	22.48610010106541
94-95	21.317654465240523	27.895958575810692	28.11581654916968	22.670570409779103
96-97	21.3649314143142	27.798027561055573	28.176130358860274	22.660910665769954
98-99	21.24321662874759	27.71601957042765	28.064936143797752	22.975827657027004
100	20.41019464833455	28.29101079860984	28.320624522738687	22.978170030316928
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1477.0
1	1106.5
2	1336.0
3	2025.5
4	1570.5
5	1243.0
6	1894.0
7	3363.5
8	5406.0
9	6697.0
10	5770.5
11	4354.0
12	4261.0
13	4470.5
14	4750.5
15	5078.5
16	5379.0
17	5775.0
18	6236.5
19	6763.0
20	7519.0
21	8624.0
22	10078.5
23	12216.5
24	15163.0
25	18906.5
26	23894.5
27	29661.5
28	36318.0
29	44618.0
30	54021.0
31	63119.0
32	72738.5
33	83180.0
34	93398.0
35	103079.0
36	114659.5
37	125092.0
38	131537.0
39	137339.0
40	139751.5
41	140379.5
42	141934.5
43	143963.5
44	146816.0
45	150376.5
46	152433.5
47	151957.0
48	151997.5
49	151556.0
50	149397.5
51	142288.0
52	132329.0
53	124822.5
54	116778.5
55	106634.5
56	95670.0
57	84571.0
58	73593.0
59	61904.5
60	50108.0
61	40090.5
62	32133.5
63	24859.5
64	18119.5
65	13172.5
66	9759.0
67	7304.0
68	5445.0
69	4090.0
70	3053.0
71	2279.5
72	1731.0
73	1368.0
74	1053.0
75	739.0
76	491.5
77	316.0
78	201.0
79	137.0
80	92.0
81	66.0
82	49.5
83	31.5
84	23.5
85	20.0
86	15.5
87	11.0
88	8.5
89	8.0
90	6.0
91	3.0
92	0.5
93	0.0
94	1.0
95	1.5
96	0.5
97	0.0
98	1.0
99	2.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0034411655995339823
2	0.06669424550463703
3	6.931844373161978E-4
4	5.941580891281696E-4
5	0.001609178158055459
6	8.417239595982402E-4
7	0.0
8	0.0012873425264443674
9	2.4756587047007066E-5
10-11	0.0
12-13	0.0
14-15	0.0
16-17	9.53128601309772E-4
18-19	0.0010521549494978003
20-21	0.003230734609634422
22-23	0.0
24-25	2.4756587047007066E-5
26-27	2.970790445640848E-4
28-29	0.001163559591209332
30-31	0.0018938789090960403
32-33	0.0026613331075532594
34-35	0.0042333763850382075
36-37	0.0012997208199678708
38-39	0.0014606386357734168
40-41	0.005136991812253966
42-43	0.006226281642322276
44-45	0.005025587170542434
46-47	0.006523360686886361
48-49	0.008169673725512331
50-51	0.007575515636384161
52-53	0.003998188808091641
54-55	0.002252849421277643
56-57	0.003305004370775443
58-59	0.0038620275793331024
60-61	0.007266058298296574
62-63	0.013207639189578268
64-65	0.0036020834153395282
66-67	0.009444637958433196
68-69	0.011251868812864711
70-71	0.005483584030912064
72-73	0.006770926557356432
74-75	0.0029088989780233303
76-77	0.003007925326211358
78-79	0.007860216387424743
80-81	0.006399577751651326
82-83	0.015930863764749045
84-85	0.01713155823652889
86-87	0.02151347414384914
88-89	0.020250888204451777
90-91	0.02301124766019307
92-93	0.03342139251345954
94-95	0.03701109763527556
96-97	0.021711526840225197
98-99	0.016945883833676335
100	0.016364104038071668
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	4039329.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.63040200436589
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.03684395374776	49.90328635827705
2	16.04682455498556	21.063190928744643
3	4.267972918560193	8.403263351665567
4	1.5851005632670647	4.161231487382571
5	0.6753167291706601	2.2160654207871953
6	0.3783757264362572	1.4899770620823323
7	0.22653425533845167	1.0407273971935578
8	0.15550054357950951	0.8164450549616503
9	0.10850210651170496	0.6408933181815352
>10	0.47584125078798634	5.355841466769602
>50	0.026811157494052322	1.2113090920395604
>100	0.015336159674047016	1.830091550544084
>500	6.783133373892777E-4	0.30770825634328863
>1k	2.2610444333554897E-4	0.3720515692139344
>5k	4.522088866710979E-5	0.25090331574143276
>10k+	9.044177733421959E-5	0.9370143700719795
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	16096	0.3984820251086257	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	15476	0.3831329411394813	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8454	0.20929218689539772	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10-11	0.0	0.0	0.0	0.0	0.0
12-13	0.0	0.0	0.0	0.0	0.0
14-15	0.0	0.0	0.0	0.0	0.0
16-17	0.0	0.0	0.0	4.951317409401413E-5	0.0
18-19	0.0	0.0	0.0	1.2378293523503532E-4	0.0
20-21	0.0	0.0	0.0	1.2378293523503532E-4	0.0
22-23	0.0	0.0	0.0	1.9805269637605653E-4	0.0
24-25	0.0	0.0	0.0	2.5994416399357416E-4	2.4756587047007066E-5
26-27	0.0	0.0	0.0	5.694015020811624E-4	2.4756587047007066E-5
28-29	0.0	0.0	0.0	9.531286013097721E-4	2.4756587047007066E-5
30-31	0.0	0.0	0.0	0.003391652425439968	2.4756587047007066E-5
32-33	0.0	0.0	0.0	0.009469394545480202	3.71348805705106E-5
34-35	0.0	0.0	0.0	0.020486075781398348	4.951317409401413E-5
36-37	0.0	0.0	0.0	0.038248926987625914	4.951317409401413E-5
38-39	0.0	0.0	0.0	0.06856336782668607	7.42697611410212E-5
40-41	0.0	1.2378293523503533E-5	0.0	0.10114303638054736	7.42697611410212E-5
42-43	0.0	2.4756587047007066E-5	0.0	0.13577750165931024	7.42697611410212E-5
44-45	0.0	2.4756587047007066E-5	0.0	0.18017844052811743	8.664805466452474E-5
46-47	0.0	2.4756587047007066E-5	0.0	0.23581886991626583	1.2378293523503532E-4
48-49	0.0	2.4756587047007066E-5	0.0	0.29524705712260624	1.7329610932904945E-4
50-51	0.0	2.4756587047007066E-5	0.0	0.35575215586549147	1.7329610932904945E-4
52-53	0.0	2.4756587047007066E-5	0.0	0.4201316604812334	1.7329610932904945E-4
54-55	0.0	2.4756587047007066E-5	0.0	0.49557735950698745	1.7329610932904945E-4
56-57	0.0	2.4756587047007066E-5	0.0	0.5889468275547746	1.7329610932904945E-4
58-59	0.0	2.4756587047007066E-5	0.0	0.6956600960208985	1.85674402852553E-4
60-61	0.0	2.4756587047007066E-5	0.0	0.8092928305666609	1.9805269637605653E-4
62-63	0.0	2.4756587047007066E-5	0.0	0.925079388185513	2.2280928342306358E-4
64-65	0.0	2.4756587047007066E-5	0.0	1.0485528660824608	2.2280928342306358E-4
66-67	0.0	2.4756587047007066E-5	0.0	1.196770552732892	2.2280928342306358E-4
68-69	0.0	2.4756587047007066E-5	0.0	1.3674548421284822	2.2280928342306358E-4
70-71	0.0	2.4756587047007066E-5	0.0	1.5583281282609067	2.4756587047007064E-4
72-73	0.0	2.4756587047007066E-5	0.0	1.7533233861366577	2.4756587047007064E-4
74-75	0.0	2.4756587047007066E-5	0.0	1.9506086283142572	2.4756587047007064E-4
76-77	0.0	2.4756587047007066E-5	0.0	2.174383418632154	2.4756587047007064E-4
78-79	0.0	2.4756587047007066E-5	0.0	2.430886417026194	2.4756587047007064E-4
80-81	0.0	2.4756587047007066E-5	0.0	2.7027013645088083	2.4756587047007064E-4
82-83	0.0	2.4756587047007066E-5	0.0	2.988231461215464	2.4756587047007064E-4
84-85	0.0	2.4756587047007066E-5	0.0	3.279257520246556	2.4756587047007064E-4
86-87	0.0	2.4756587047007066E-5	0.0	3.596859775472609	2.4756587047007064E-4
88	0.0	2.4756587047007066E-5	0.0	3.8494264765261756	2.4756587047007064E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	9655	0.0	42.22457	1
GTATCAA	14895	0.0	32.326443	1
TCAACGC	18030	0.0	26.428455	4
ATCAACG	18045	0.0	26.355055	3
CAACGCA	18185	0.0	26.125021	5
TATCAAC	18665	0.0	25.544197	2
AACGCAG	18795	0.0	25.302124	6
GTACATG	15945	0.0	22.530315	1
ACGCAGA	21180	0.0	22.275179	7
CGCAGAG	21485	0.0	21.958961	8
TACCTGG	3195	0.0	21.927935	2
TACATGG	16175	0.0	21.511454	2
GTACCTG	3430	0.0	21.24888	1
ACATGGG	15800	0.0	21.147198	3
CATGGGG	7755	0.0	20.60282	4
GAGTACT	13640	0.0	19.9646	12-13
GCAGAGT	23650	0.0	19.551138	9
ATGGGGG	4595	0.0	19.021564	5
AGTACTT	14660	0.0	18.142784	12-13
AGAGTAC	21800	0.0	18.095795	10-11
>>END_MODULE