FastQCFastQC Report
Mon 6 Jun 2016
ERR1211522_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211522_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6058990
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT245550.4052655640626573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT224500.3705238001713157No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT133160.21977260236442048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121700.051.7635571
GTATCAA206500.036.890711
ATCAACG251100.029.963
TCAACGC250350.029.954674
CAACGCA251150.029.799935
TATCAAC260150.028.9012032
AACGCAG259550.028.8855066
TACCTGG51100.026.1329692
GTACATG224650.025.6108111
ACGCAGA293200.025.4120457
CGCAGAG299350.024.686978
TACATGG230650.024.4839132
GTACCTG56350.024.4811761
ACATGGG235250.023.1899493
GCAGAGT328650.021.7457719
GAGTACT197300.021.70825812-13
CATGGGG132350.021.1948174
CAGAGTA316050.020.27834310-11
ACCTGGG64600.019.7848873
GTACTTT217800.019.51648714-15
Produced by FastQC (version 0.11.5)