FastQCFastQC Report
Mon 6 Jun 2016
ERR1211522_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211522_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6058990
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT260750.4303522534283767No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241420.398449246491577No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT160570.26501116522720786No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA123150.048.839871
GTATCAA206100.037.0549661
TCAACGC256700.029.0896744
ATCAACG257850.028.8872723
CAACGCA260250.028.7109245
AACGCAG267950.027.955336
TATCAAC270150.027.8504282
TACCTGG50950.024.9193342
ACGCAGA304500.024.5065677
CGCAGAG313800.023.7053998
GTACATG227650.023.549171
GTACCTG54650.023.4915071
TACATGG231500.022.8923192
ACATGGG233000.021.6415423
GCAGAGT343850.021.1825589
GAGTACT204200.021.09164212-13
CATGGGG135100.020.8011174
ACCTGGG63250.019.4663773
AGAGTAC320850.019.39854810-11
AGTACTT216900.019.29336512-13
Produced by FastQC (version 0.11.5)