FastQCFastQC Report
Mon 6 Jun 2016
ERR1211521_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211521_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4850240
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181600.37441446196476874No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164010.3381482153460447No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103620.2136389127135977No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51080.10531437619581711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA98000.048.4805561
TACCTGG50950.039.871582
GTACCTG57550.036.8188061
GTATCAA156850.035.0339281
ACCTGGG61550.032.6754383
TCAACGC190200.028.4849574
ATCAACG189400.028.4818153
GTACATG180800.028.4137671
TATAACG5300.028.3921322
CAACGCA191850.028.116645
TACATGG183450.027.3508342
TATCAAC198050.027.2340662
AACGCAG199250.027.1594546
ACATGGG186350.025.9472733
CATGGGG114550.025.760924
TAACGCA6250.024.8102264
ACGCAGA221250.024.374437
ATGGGGG72700.024.2370745
CGCAGAG225400.023.8435218
ATAACGC7150.021.6877083
Produced by FastQC (version 0.11.5)