FastQCFastQC Report
Mon 6 Jun 2016
ERR1211521_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211521_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4850240
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188790.38923847067361617No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT177790.3665591805766313No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT119460.24629709045325593No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA102600.047.3470761
TACCTGG52150.042.5621382
GTACCTG57400.038.915441
GTATCAA167000.035.8469431
ACCTGGG61850.034.115113
TATAACG5300.030.1674632
TCAACGC206150.028.814024
ATCAACG208750.028.3876023
CAACGCA208750.028.3425795
TATCAAC212050.028.0092072
AACGCAG216350.027.4332736
GTACATG184850.025.5165351
TAACGCA5600.025.1752244
TACATGG190400.024.1550332
ACGCAGA244150.024.0396397
CATGGGG111750.023.8438114
ACATGGG188400.023.5467643
CGCAGAG249500.023.486498
CCTGGGG58800.023.1771934
ATGGGGG68550.022.0058295
Produced by FastQC (version 0.11.5)