##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211521_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 4850240 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.06068978029953 33.0 31.0 34.0 30.0 34.0 2 32.28534835389589 34.0 31.0 34.0 30.0 34.0 3 32.29486046051329 34.0 31.0 34.0 30.0 34.0 4 35.953446633568646 37.0 35.0 37.0 35.0 37.0 5 35.86617198324207 37.0 35.0 37.0 35.0 37.0 6 35.883765751797846 37.0 35.0 37.0 35.0 37.0 7 35.80710439071056 37.0 35.0 37.0 35.0 37.0 8 35.79830441380221 37.0 35.0 37.0 35.0 37.0 9 37.439902149172 39.0 37.0 39.0 34.0 39.0 10-11 37.39706313502012 39.0 37.0 39.0 34.0 39.0 12-13 37.304011244804386 39.0 37.0 39.0 34.0 39.0 14-15 38.50802207725803 40.0 38.0 41.0 34.0 41.0 16-17 38.42923690374084 40.0 38.0 41.0 34.0 41.0 18-19 38.41968211882299 40.0 38.0 41.0 33.5 41.0 20-21 38.354926663422845 40.0 38.0 41.0 34.0 41.0 22-23 38.24271788612522 40.0 38.0 41.0 33.5 41.0 24-25 38.12883321237712 40.0 37.5 41.0 33.0 41.0 26-27 37.94282643745464 40.0 37.0 41.0 33.0 41.0 28-29 37.97678073662334 40.0 37.0 41.0 33.0 41.0 30-31 37.888725300191325 40.0 37.0 41.0 33.0 41.0 32-33 37.68856716368674 40.0 37.0 41.0 32.5 41.0 34-35 37.56358035891007 40.0 37.0 41.0 32.0 41.0 36-37 37.49862676898462 40.0 36.5 41.0 32.0 41.0 38-39 37.39734466748037 40.0 36.0 41.0 31.5 41.0 40-41 37.224714859470865 40.0 36.0 41.0 31.0 41.0 42-43 37.06909853945372 39.0 36.0 41.0 31.0 41.0 44-45 36.87525606980273 39.0 35.5 41.0 30.0 41.0 46-47 36.60143972257043 39.0 35.0 41.0 30.0 41.0 48-49 36.36801601570231 39.0 35.0 40.5 29.0 41.0 50-51 36.15861050174837 39.0 35.0 40.0 28.5 41.0 52-53 35.950270502078254 39.0 35.0 40.0 28.0 41.0 54-55 35.74281148974072 38.0 34.5 40.0 27.5 41.0 56-57 35.53345834432935 38.0 34.0 40.0 27.0 41.0 58-59 35.26188508197532 38.0 34.0 40.0 26.0 41.0 60-61 34.9365034513756 37.5 33.5 40.0 26.0 41.0 62-63 34.59478479415452 37.0 33.0 40.0 26.0 41.0 64-65 34.271319460480306 36.5 33.0 39.5 25.5 41.0 66-67 33.92373367091113 36.0 32.0 39.0 25.0 40.5 68-69 33.517401716203736 35.5 32.0 39.0 23.5 40.0 70-71 33.12175335653494 35.0 31.5 38.0 23.0 40.0 72-73 32.70797187355677 35.0 31.0 37.5 22.5 39.5 74-75 32.29777072887114 35.0 31.0 37.0 22.0 39.0 76-77 30.78415470162301 33.5 29.5 35.5 20.5 37.5 78-79 31.614257748070198 34.0 30.0 36.0 21.0 38.0 80-81 31.59126239113941 34.0 30.5 36.0 21.0 37.5 82-83 31.313294393679488 34.0 30.0 35.0 20.0 37.0 84-85 31.011322017054827 34.0 30.0 35.0 20.0 36.5 86-87 30.712359697664446 34.0 30.0 35.0 19.5 36.0 88-89 30.433437623705217 34.0 29.0 35.0 19.0 36.0 90-91 30.14745229102065 34.0 29.0 35.0 18.0 35.0 92-93 29.93221335439071 34.0 29.0 35.0 18.0 35.0 94-95 29.708859870851754 34.0 29.0 35.0 17.5 35.0 96-97 29.49598102361945 33.5 29.0 35.0 17.0 35.0 98-99 29.325726768159925 33.0 27.5 35.0 15.5 35.0 100 27.439851017681598 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.33212204254176214 1101 2 0.3031789561638405 1101 3 0.31559936415541046 1101 4 0.23268794300854978 1101 5 0.18022069544636565 1101 6 0.20607012070365727 1101 7 0.20487967502815962 1101 8 0.22114093225881248 1101 9 0.20389258558739698 1101 10-11 0.22306115419586803 1101 12-13 0.25412879123414456 1101 14-15 0.3678041358036026 1101 16-17 0.3330361788495324 1101 18-19 0.4026153128898571 1101 20-21 0.3167742078114486 1101 22-23 0.3556530183306279 1101 24-25 0.3248823754680217 1101 26-27 0.3085372447871606 1101 28-29 0.3613829757879188 1101 30-31 0.39390397687881773 1101 32-33 0.4278939479546011 1101 34-35 0.3821866123843378 1101 36-37 0.4277110772440551 1101 38-39 0.3884802383206676 1101 40-41 0.3846941771750494 1101 42-43 0.5317871754161843 1101 44-45 0.5026121498102398 1101 46-47 0.48746727146905755 1101 48-49 0.5424200134098598 1101 50-51 0.560588698074298 1101 52-53 0.4488496863634168 1101 54-55 0.3508428816042013 1101 56-57 0.36615095559084665 1101 58-59 0.4350594972796671 1101 60-61 0.4012901069347592 1101 62-63 0.46293258180261176 1101 64-65 0.49132877528820273 1101 66-67 0.41931792211273233 1101 68-69 0.3706744015442922 1101 70-71 0.3194864109935551 1101 72-73 0.16534442307044372 1101 74-75 0.20900799205823972 1101 76-77 0.24114459013601675 1101 78-79 0.2962268165126787 1101 80-81 0.31685775171448327 1101 82-83 0.3559470639365827 1101 84-85 0.31764591052570523 1101 86-87 0.24567880945911824 1101 88-89 0.1977785519501296 1101 90-91 0.1992328019982601 1101 92-93 0.11677917395750015 1101 94-95 0.06250562567019458 1101 96-97 0.020826179721762372 1101 98-99 0.09748394629833257 1101 100 0.1392912978014067 1102 1 0.33450955315937136 1102 2 0.2584241207569846 1102 3 0.3182066482397019 1102 4 0.18845764749656269 1102 5 0.21526716816034508 1102 6 0.16069294100135068 1102 7 0.19694012418887752 1102 8 0.22152829601358803 1102 9 0.2306259967267863 1102 10-11 0.20040370759900128 1102 12-13 0.26629960625519544 1102 14-15 0.39407133555112495 1102 16-17 0.36270757585364066 1102 18-19 0.34253648608437004 1102 20-21 0.33006227406012556 1102 22-23 0.2984184872318991 1102 24-25 0.28452139494505957 1102 26-27 0.3502666536538257 1102 28-29 0.3391983025395007 1102 30-31 0.31457815021550317 1102 32-33 0.47937838231654695 1102 34-35 0.4465734157688459 1102 36-37 0.5028018379571009 1102 38-39 0.5168956455864162 1102 40-41 0.4732720501532768 1102 42-43 0.3722872590030164 1102 44-45 0.41938039660646353 1102 46-47 0.4408236862242916 1102 48-49 0.4134422568803231 1102 50-51 0.4560173058093824 1102 52-53 0.4177525220140055 1102 54-55 0.3862217747062786 1102 56-57 0.42371379283505917 1102 58-59 0.3942379819780655 1102 60-61 0.37850673326622086 1102 62-63 0.3925572118462739 1102 64-65 0.3878584897545565 1102 66-67 0.2924760824525876 1102 68-69 0.23865351160757342 1102 70-71 0.3532062748173317 1102 72-73 0.39400501836489354 1102 74-75 0.3273978753960236 1102 76-77 0.35985684261094164 1102 78-79 0.3702718304932908 1102 80-81 0.462051561185465 1102 82-83 0.4609818553262528 1102 84-85 0.3612544866904912 1102 86-87 0.28578689425834725 1102 88-89 0.26173669291658186 1102 90-91 0.19659104160237462 1102 92-93 0.25469337793748537 1102 94-95 0.3210615577570728 1102 96-97 0.36458047298602025 1102 98-99 0.2635344139939768 1102 100 0.2897056124493069 1103 1 0.2868090249885995 1103 2 0.25182100528247275 1103 3 0.3069639102866688 1103 4 0.12840707085192093 1103 5 0.14032735182074418 1103 6 0.22391061501945586 1103 7 0.23535190879486834 1103 8 0.24174439622252208 1103 9 0.2301726102838444 1103 10-11 0.2599641595262412 1103 12-13 0.2860446994155481 1103 14-15 0.35634140299467987 1103 16-17 0.3957623693983052 1103 18-19 0.3621583038356846 1103 20-21 0.35733413625872856 1103 22-23 0.3954648848332951 1103 24-25 0.39298172913773755 1103 26-27 0.3537250072491389 1103 28-29 0.4687282006118778 1103 30-31 0.5363754416668627 1103 32-33 0.6081503190241548 1103 34-35 0.5522412453266483 1103 36-37 0.614578184290103 1103 38-39 0.6268257688721519 1103 40-41 0.592937265529045 1103 42-43 0.6965756463700075 1103 44-45 0.6868316620378323 1103 46-47 0.710265667110761 1103 48-49 0.6439262488010016 1103 50-51 0.6878396722085398 1103 52-53 0.7219467251789027 1103 54-55 0.8539403261410641 1103 56-57 0.8297625647442288 1103 58-59 0.7744489405493411 1103 60-61 0.7287007664197986 1103 62-63 0.688658635639392 1103 64-65 0.6436949368072149 1103 66-67 0.7097841632554491 1103 68-69 0.6721414382232567 1103 70-71 0.7756870530423967 1103 72-73 0.734151038809614 1103 74-75 0.5869505494778053 1103 76-77 0.6647237261624852 1103 78-79 0.6923082906925799 1103 80-81 0.6966575530861867 1103 82-83 0.6708529908813645 1103 84-85 0.6116024592367602 1103 86-87 0.5403645004646584 1103 88-89 0.7477744106489332 1103 90-91 0.690879005661845 1103 92-93 0.6765883398552965 1103 94-95 0.726788994519918 1103 96-97 0.6944107989431352 1103 98-99 0.7100894995519944 1103 100 0.8378217735388098 1104 1 0.26396332605450823 1104 2 0.2418269683398364 1104 3 0.28034475423728367 1104 4 0.09482106802742862 1104 5 0.15912822699465323 1104 6 0.12364190617855542 1104 7 0.17685180404408385 1104 8 0.1635187364504418 1104 9 0.1666237535663342 1104 10-11 0.22342211310182591 1104 12-13 0.296945651249402 1104 14-15 0.38886693855778987 1104 16-17 0.2688622043576956 1104 18-19 0.28332693539208975 1104 20-21 0.2504567422834185 1104 22-23 0.3575457073341113 1104 24-25 0.32696368230168105 1104 26-27 0.3978598549246968 1104 28-29 0.4350875150316824 1104 30-31 0.3628332454200276 1104 32-33 0.32999742617799654 1104 34-35 0.5139746796611675 1104 36-37 0.4161909855509762 1104 38-39 0.4234039225699746 1104 40-41 0.5339947370358971 1104 42-43 0.5701878719352607 1104 44-45 0.5400656092313625 1104 46-47 0.4620842330582633 1104 48-49 0.5014097331167946 1104 50-51 0.4860774093750422 1104 52-53 0.49269949021452675 1104 54-55 0.4931270402022321 1104 56-57 0.5360070960349503 1104 58-59 0.5697129559027019 1104 60-61 0.5319506326795249 1104 62-63 0.5282946018479464 1104 64-65 0.6567325583349586 1104 66-67 0.6023492376430752 1104 68-69 0.5735842371809312 1104 70-71 0.47436298436501545 1104 72-73 0.5607787199561329 1104 74-75 0.6341697856221629 1104 76-77 0.6142342461045835 1104 78-79 0.6010846856181047 1104 80-81 0.7726459213423489 1104 82-83 0.5242616557248567 1104 84-85 0.5678540167064305 1104 86-87 0.6890002628619776 1104 88-89 0.5892740317904241 1104 90-91 0.5108215290892097 1104 92-93 0.5641389951358242 1104 94-95 0.6302346708453292 1104 96-97 0.48275198089424265 1104 98-99 0.6422973165074417 1104 100 0.7452260684806618 1105 1 0.2380795473847428 1105 2 0.12441991511072814 1105 3 0.2168185396099105 1105 4 0.1604907644475091 1105 5 0.14637693770858107 1105 6 0.16711816383738665 1105 7 0.1794074423371086 1105 8 0.19753140534965752 1105 9 0.1762509199433282 1105 10-11 0.22294003842227283 1105 12-13 0.24696360149645358 1105 14-15 0.34367546942773686 1105 16-17 0.3575161364787931 1105 18-19 0.3163242445901062 1105 20-21 0.2866554279659539 1105 22-23 0.35836627533153376 1105 24-25 0.40158496713394953 1105 26-27 0.43014346614815224 1105 28-29 0.4264414879595648 1105 30-31 0.37865105671714616 1105 32-33 0.4479791827899646 1105 34-35 0.5455767174789017 1105 36-37 0.5296549455125543 1105 38-39 0.4549611062437222 1105 40-41 0.5030218941156548 1105 42-43 0.5138695000729996 1105 44-45 0.5308115516835841 1105 46-47 0.5528170027193653 1105 48-49 0.5894815577431771 1105 50-51 0.6250697506373228 1105 52-53 0.5895715398434476 1105 54-55 0.5162325418558922 1105 56-57 0.597125067449852 1105 58-59 0.6020090218386756 1105 60-61 0.6304544729741721 1105 62-63 0.6590514405416243 1105 64-65 0.7631940494461205 1105 66-67 0.721659113261218 1105 68-69 0.6846866118599806 1105 70-71 0.8005509260112689 1105 72-73 0.6677851262136016 1105 74-75 0.6329948166326815 1105 76-77 0.6397011440727276 1105 78-79 0.7752577244879006 1105 80-81 0.7359665352933611 1105 82-83 0.6786356356624275 1105 84-85 0.6078171521316911 1105 86-87 0.6320826045911829 1105 88-89 0.6829029086375051 1105 90-91 0.7154517514231777 1105 92-93 0.7166448884481049 1105 94-95 0.8548220840132217 1105 96-97 0.7519515215026011 1105 98-99 0.8370160099039943 1105 100 0.9977933111299677 1106 1 0.25895657746311684 1106 2 0.20675323616031704 1106 3 0.20546678517984418 1106 4 0.18105417441346816 1106 5 0.18700427303337364 1106 6 0.13591534015153428 1106 7 0.15172013578425236 1106 8 0.153764162799078 1106 9 0.17038632873088488 1106 10-11 0.19516784116340347 1106 12-13 0.22644587098164948 1106 14-15 0.28828042031803847 1106 16-17 0.34807798918279076 1106 18-19 0.322313234950812 1106 20-21 0.2993154362830097 1106 22-23 0.3289026797038659 1106 24-25 0.37753261208465005 1106 26-27 0.2969895967087979 1106 28-29 0.40780371247646485 1106 30-31 0.43688214767949773 1106 32-33 0.4822847827585264 1106 34-35 0.4172134214790546 1106 36-37 0.383612390794589 1106 38-39 0.393950771184862 1106 40-41 0.4796014643630997 1106 42-43 0.4382175111306168 1106 44-45 0.523405414477125 1106 46-47 0.5856146039066061 1106 48-49 0.5928139375128652 1106 50-51 0.5308305779958573 1106 52-53 0.5244001966242351 1106 54-55 0.3907981500296174 1106 56-57 0.41246187412301083 1106 58-59 0.5677249417340064 1106 60-61 0.5662550374344306 1106 62-63 0.635446807047451 1106 64-65 0.653311998578971 1106 66-67 0.7076774076978509 1106 68-69 0.6047812244398401 1106 70-71 0.5906062804855594 1106 72-73 0.5562268160533108 1106 74-75 0.5808278694793856 1106 76-77 0.524017086162722 1106 78-79 0.6512379796369174 1106 80-81 0.6454165421146847 1106 82-83 0.5817511105878204 1106 84-85 0.5664760426045667 1106 86-87 0.550921687824701 1106 88-89 0.6172661524722152 1106 90-91 0.6029595735702955 1106 92-93 0.6229111091984869 1106 94-95 0.8518063486747529 1106 96-97 0.8273064803820027 1106 98-99 0.8976544761205325 1106 100 0.930173982872784 1107 1 0.18312304416394198 1107 2 0.12642023975784866 1107 3 0.2116320641298799 1107 4 0.1398470045493525 1107 5 0.13397055184351103 1107 6 0.1308738846843127 1107 7 0.17817677500541151 1107 8 0.16829511704843725 1107 9 0.18457263142574476 1107 10-11 0.13809465551758393 1107 12-13 0.1951888934090178 1107 14-15 0.21362792080676485 1107 16-17 0.21567805626013836 1107 18-19 0.17177879342067115 1107 20-21 0.19251885203932773 1107 22-23 0.23973914532070495 1107 24-25 0.23439845090816647 1107 26-27 0.27693865869141376 1107 28-29 0.32643073927312116 1107 30-31 0.30391960815343566 1107 32-33 0.27935589308315656 1107 34-35 0.3738871541202471 1107 36-37 0.43040833576522886 1107 38-39 0.42290845038282043 1107 40-41 0.42300819674208867 1107 42-43 0.3647196406510389 1107 44-45 0.42028071268873646 1107 46-47 0.43408141405456035 1107 48-49 0.5390872072833091 1107 50-51 0.4362538369727744 1107 52-53 0.501467434251289 1107 54-55 0.5272891988172788 1107 56-57 0.6480349175537441 1107 58-59 0.7524143986391323 1107 60-61 0.6368822013505238 1107 62-63 0.6613667811800568 1107 64-65 0.6880534774861999 1107 66-67 0.7025852273042617 1107 68-69 0.6652460315322557 1107 70-71 0.7383038320697963 1107 72-73 0.6948226018542201 1107 74-75 0.6797618585153842 1107 76-77 0.5366165556587426 1107 78-79 0.6814614657873932 1107 80-81 0.7531539695131819 1107 82-83 0.6996196260197785 1107 84-85 0.6222540054729961 1107 86-87 0.5339770145905085 1107 88-89 0.6054750391087218 1107 90-91 0.5630944138585825 1107 92-93 0.5242583076538558 1107 94-95 0.7455052010406646 1107 96-97 0.8834330323562938 1107 98-99 0.9527255327091702 1107 100 1.1024538992299284 1108 1 0.24212453154667202 1108 2 0.1769487850846474 1108 3 0.241892096331064 1108 4 0.16964524929488078 1108 5 0.15223639885553553 1108 6 0.12717720433043667 1108 7 0.16605837284182456 1108 8 0.14398191998219545 1108 9 0.18680234012083474 1108 10-11 0.2002246216232919 1108 12-13 0.2499238533171777 1108 14-15 0.2876209230638196 1108 16-17 0.32206599119285073 1108 18-19 0.30981579901575174 1108 20-21 0.2684014892651305 1108 22-23 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2316 88-89 -0.05954560087827687 2316 90-91 0.08800198887206179 2316 92-93 0.19721224625363476 2316 94-95 0.0952952971224299 2316 96-97 0.13348536455561089 2316 98-99 0.055891722177783265 2316 100 -0.06274948449238238 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 2.0 10 12.0 11 34.0 12 68.0 13 181.0 14 549.0 15 1481.0 16 3288.0 17 6782.0 18 11851.0 19 18165.0 20 24734.0 21 33526.0 22 43630.0 23 49700.0 24 53762.0 25 59533.0 26 69258.0 27 81306.0 28 97681.0 29 116633.0 30 140574.0 31 169072.0 32 204920.0 33 255312.0 34 333810.0 35 446119.0 36 637765.0 37 959340.0 38 898520.0 39 132618.0 40 14.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.11776974949248 20.06190008647291 14.21714390337137 23.60318626066324 2 15.142006144319083 21.142011508374424 40.02435281124983 23.691629536056663 3 17.073072671863567 27.251768432594208 31.042788141270076 24.632370754272145 4 12.023575047116488 17.664749981083304 37.74685771532096 32.56481725647925 5 12.455668997379675 38.12518800922279 35.234768495313865 14.18437449808367 6 30.30386125788033 37.67760839898173 18.286353551508213 13.732176791629719 7 26.62480619515735 33.08807399221482 22.835900903872798 17.45121890875503 8 25.915845375986223 36.105980156245394 20.26435659956484 17.71381786820354 9 26.714910337408114 15.33340934333339 20.467733651888082 37.48394666737041 10-11 24.629544105033975 26.372756812034044 29.073272662796068 19.92442642013591 12-13 25.09398503991555 24.336414280530448 28.900404928415913 21.669195751138087 14-15 22.63897250445339 25.59038315629742 26.571468628356538 25.199175710892657 16-17 22.043876917342033 28.36789957700748 27.288749091790777 22.299474413859706 18-19 21.907970608637726 27.398485897644846 29.298386736795727 21.3951567569217 20-21 22.635389519348134 26.72233241513542 29.27904281991225 21.363235245604194 22-23 22.474609503859604 26.54582041301049 29.044573052055156 21.93499703107475 24-25 22.15677987068681 26.693225489872667 29.131424424358382 22.01857021508214 26-27 22.077111299326464 26.706195327402625 29.165488029860814 22.051205343410093 28-29 22.10505057835795 26.899752731693166 29.218905953716934 21.77629073623195 30-31 22.555067405376246 26.731457529473968 29.182598670005245 21.530876395144542 32-33 22.325595828043923 26.823431851597256 29.064592289765635 21.78638003059319 34-35 22.495527894064903 26.949479429549793 28.98756593727423 21.56742673911107 36-37 22.497870185650537 26.928451020867644 28.886973041045007 21.686705752436815 38-39 22.438271989931895 27.0366586233035 28.81520507998846 21.709864306776147 40-41 22.470328623541945 26.84746038790702 28.91049025232461 21.771720736226424 42-43 22.310293294712878 27.067347720608158 28.653637348467008 21.968721636211956 44-45 22.26648215843701 27.161379786988043 28.63490267390198 21.937235380672966 46-47 22.235905089174263 26.965394637709068 28.46485586837946 22.33384440473721 48-49 22.217073140557215 27.11495636969467 28.4576707685202 22.210299721227916 50-51 22.309241997003827 27.347196220588426 28.28881190377734 22.054749878630407 52-53 22.362656882047464 27.429572535273266 28.044358617345228 22.163411965334046 54-55 22.1917775456427 27.307407581896253 28.293790361546883 22.20702451091416 56-57 22.34395911196479 27.35834740685958 27.94792008367623 22.349773397499405 58-59 22.260585787309424 27.357266507329353 28.22112712181717 22.16102058354405 60-61 22.57855633035778 27.443346131161363 28.12384308455938 21.854254453921477 62-63 22.403450662825264 27.45705384278641 28.175770096578724 21.9637253978096 64-65 22.270331478618406 27.18732084671329 28.1521519837388 22.390195690929502 66-67 22.228146874325034 27.27130078245616 28.04252965461924 22.458022688599563 68-69 22.020248779557395 27.359998350404922 28.150474000835107 22.469278869202576 70-71 22.121894812783356 27.219049777118993 28.135285095833318 22.523770314264333 72-73 22.045859379333198 27.550659156153934 27.760023736508266 22.643457728004606 74-75 22.128073077165737 27.58594348247234 27.717291127245463 22.568692313116458 76-77 21.993170474031864 27.392462903472225 27.91449154731731 22.6998750751786 78-79 21.942087806455152 27.368763111111292 27.90201187902251 22.787137203411042 80-81 21.95451271770491 27.61139190316808 27.593433032898545 22.840662346228463 82-83 22.121579637672262 27.531286959317185 27.66275399888793 22.684379404122627 84-85 22.018172589387262 27.635463139968653 27.555227179019298 22.791137091624787 86-87 21.867802960313583 27.615917707109766 27.702374981852735 22.81390435072391 88-89 21.759768641918868 27.405906382587354 27.747720047895776 23.086604927598007 90-91 22.12166012391496 27.37438370413509 27.689873156680413 22.81408301526954 92-93 21.992851662720575 27.440055850252026 27.54518755620086 23.021904930826533 94-95 21.761812931834587 27.31334433329896 27.612457461070434 23.312385273796018 96-97 21.740851923899026 27.237740166448454 27.72826548934265 23.29314242030987 98-99 21.662299130452 27.190614292525122 27.601735961608576 23.54535061541431 100 20.6912785446507 27.78134292348363 27.796437512114853 23.730941019750816 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 2425.0 1 1777.5 2 2300.0 3 3270.5 4 2327.5 5 1746.5 6 2496.0 7 4756.0 8 7409.5 9 8734.0 10 7488.5 11 5677.0 12 5549.5 13 5873.5 14 6253.5 15 6623.5 16 7079.0 17 7458.0 18 8036.0 19 8541.0 20 9101.5 21 10235.5 22 11770.0 23 14157.0 24 17097.5 25 20881.0 26 26048.5 27 32253.5 28 39033.0 29 46867.0 30 56643.5 31 66085.0 32 75570.0 33 86484.5 34 97987.5 35 108331.0 36 120581.0 37 132194.0 38 139859.5 39 146847.0 40 150421.5 41 153098.0 42 156086.5 43 160243.5 44 164441.0 45 169055.0 46 174153.5 47 176546.5 48 180743.0 49 184407.0 50 185384.0 51 181896.0 52 174478.5 53 168413.0 54 160967.5 55 149316.5 56 135732.5 57 122174.0 58 108072.5 59 92178.0 60 75570.0 61 61358.0 62 49672.5 63 38749.0 64 28925.5 65 21609.5 66 16189.5 67 12099.0 68 9050.0 69 6729.0 70 5137.0 71 3906.0 72 2937.5 73 2199.5 74 1694.5 75 1240.5 76 837.5 77 575.0 78 411.5 79 261.0 80 169.0 81 131.5 82 96.5 83 68.5 84 48.5 85 29.0 86 24.5 87 22.5 88 13.5 89 8.0 90 5.5 91 3.5 92 3.5 93 3.5 94 3.0 95 2.0 96 2.5 97 2.5 98 3.0 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003422511050999538 2 0.06517203272415387 3 8.453189945239823E-4 4 5.566734841987201E-4 5 0.0016287853796925513 6 6.803787029095468E-4 7 0.0 8 0.001010259286138418 9 2.061753645180445E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.422313122649601E-4 18-19 8.143926898462756E-4 20-21 0.003298805832288711 22-23 0.0 24-25 0.0 26-27 8.24701458072178E-5 28-29 8.865540674275912E-4 30-31 0.0014535363198522134 32-33 0.0029070726397044267 34-35 0.003628686415517583 36-37 0.0010721118954938314 38-39 0.001474153856304018 40-41 0.004896664907303555 42-43 0.00630896615425216 44-45 0.0047523421521409245 46-47 0.006144025862637725 48-49 0.008247014580721778 50-51 0.00792744276571881 52-53 0.0036905390248729956 54-55 0.0019483571946955203 56-57 0.00241225176486112 58-59 0.0031338655406742758 60-61 0.0067728607244177605 62-63 0.012803490136570561 64-65 0.0034740548921290493 66-67 0.00922634756218249 68-69 0.011648908095269512 70-71 0.0047523421521409245 72-73 0.006741934419740055 74-75 0.002762749884541796 76-77 0.0025359569835719468 78-79 0.007133667612324338 80-81 0.005546117305535396 82-83 0.015164198060302172 84-85 0.01636001517450683 86-87 0.021112357326647756 88-89 0.01980314376195817 90-91 0.022844230388599326 92-93 0.03216335686481494 94-95 0.03587451342613974 96-97 0.021669030810846472 98-99 0.016988850036286862 100 0.016906379890479648 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 4850240.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 61.883597856064924 #Duplication Level Percentage of deduplicated Percentage of total 1 75.42885889011168 46.67809170297539 2 15.588967300692836 19.294027668548427 3 4.518941036261983 8.389449894699174 4 1.7344293773864243 4.293309203997063 5 0.8375491896942249 2.591527861985522 6 0.48726411245647533 1.8092193830969348 7 0.30702855065106616 1.3300021951174725 8 0.20200016997282735 1.000039782836417 9 0.14018177917655336 0.7807457564378572 >10 0.6928592621769083 7.401564746199166 >50 0.03897178844306795 1.6409790908870774 >100 0.021096297428430763 2.398899994220535 >500 0.0013049912119529486 0.5440714298565634 >1k 4.2096487363909386E-4 0.5818971876005986 >5k 0.0 0.0 >10k+ 1.2628946209172778E-4 1.266174101541812 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 18879 0.38923847067361617 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17779 0.3665591805766313 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11946 0.24629709045325593 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 4.123507290360889E-5 10-11 0.0 0.0 0.0 0.0 4.123507290360889E-5 12-13 0.0 1.0308768225902223E-5 0.0 0.0 6.185260935541334E-5 14-15 0.0 2.0617536451804446E-5 0.0 0.0 1.2370521871082668E-4 16-17 0.0 2.0617536451804446E-5 0.0 2.0617536451804446E-5 1.4432275516263114E-4 18-19 0.0 2.0617536451804446E-5 0.0 3.092630467770667E-5 1.6494029161443556E-4 20-21 0.0 2.0617536451804446E-5 0.0 5.154384112951111E-5 1.6494029161443556E-4 22-23 0.0 2.0617536451804446E-5 0.0 8.24701458072178E-5 1.6494029161443556E-4 24-25 0.0 2.0617536451804446E-5 0.0 1.8555782806624002E-4 1.6494029161443556E-4 26-27 0.0 2.0617536451804446E-5 0.0 4.0204196081018674E-4 1.6494029161443556E-4 28-29 0.0 4.123507290360889E-5 0.0 9.174803721052979E-4 1.6494029161443556E-4 30-31 0.0 4.123507290360889E-5 0.0 0.0034637461239031466 1.6494029161443556E-4 32-33 0.0 4.123507290360889E-5 0.0 0.010453090981064854 1.6494029161443556E-4 34-35 0.0 4.123507290360889E-5 0.0 0.021658722042620572 1.8555782806624002E-4 36-37 0.0 4.123507290360889E-5 0.0 0.04015265223988916 1.9586659629214223E-4 38-39 0.0 4.123507290360889E-5 0.0 0.07099648677178862 2.0617536451804448E-4 40-41 0.0 4.123507290360889E-5 0.0 0.10703594048954279 2.267929009698489E-4 42-43 0.0 4.123507290360889E-5 0.0 0.14218884013986938 2.267929009698489E-4 44-45 0.0 4.123507290360889E-5 0.0 0.18483621429042688 2.267929009698489E-4 46-47 0.0 4.123507290360889E-5 0.0 0.23655530447977835 2.267929009698489E-4 48-49 0.0 4.123507290360889E-5 0.0 0.2942844065448308 2.8864551032526227E-4 50-51 0.0 4.123507290360889E-5 0.0 0.35695140859009034 2.8864551032526227E-4 52-53 0.0 4.123507290360889E-5 0.0 0.42170078181698223 3.608068879065778E-4 54-55 0.0 4.123507290360889E-5 0.0 0.500274213234809 4.329682654878934E-4 56-57 0.0 4.123507290360889E-5 0.0 0.5917026786303358 4.329682654878934E-4 58-59 0.0 5.154384112951111E-5 0.0 0.6971716863495414 4.432770337137956E-4 60-61 0.0 6.185260935541334E-5 0.0 0.8098568318268786 4.535858019396978E-4 62-63 0.0 6.185260935541334E-5 0.0 0.9298302764399288 4.742033383915023E-4 64-65 0.0 6.185260935541334E-5 0.0 1.0531953057993007 4.742033383915023E-4 66-67 0.0 6.185260935541334E-5 0.0 1.2008168667942205 4.742033383915023E-4 68-69 0.0 6.185260935541334E-5 0.0 1.3650870884739725 4.742033383915023E-4 70-71 0.0 6.185260935541334E-5 0.0 1.5485110015174506 4.948208748433067E-4 72-73 0.0 6.185260935541334E-5 0.0 1.7318524444151218 5.772910206505245E-4 74-75 0.0 6.185260935541334E-5 0.0 1.9212245167249455 5.772910206505245E-4 76-77 0.0 6.185260935541334E-5 0.0 2.1391621857887446 5.772910206505245E-4 78-79 0.0 6.185260935541334E-5 0.0 2.390551807745596 5.772910206505245E-4 80-81 0.0 6.185260935541334E-5 0.0 2.653270765982714 6.082173253282312E-4 82-83 0.0 6.185260935541334E-5 0.0 2.928308702249786 6.185260935541334E-4 84-85 0.0 6.185260935541334E-5 0.0 3.2161501286534273 6.185260935541334E-4 86-87 0.0 6.185260935541334E-5 0.0 3.5206299069736753 6.185260935541334E-4 88 0.0 8.247014580721778E-5 0.0 3.7671125552549976 6.185260935541334E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 10260 0.0 47.347076 1 TACCTGG 5215 0.0 42.562138 2 GTACCTG 5740 0.0 38.91544 1 GTATCAA 16700 0.0 35.846943 1 ACCTGGG 6185 0.0 34.11511 3 TATAACG 530 0.0 30.167463 2 TCAACGC 20615 0.0 28.81402 4 ATCAACG 20875 0.0 28.387602 3 CAACGCA 20875 0.0 28.342579 5 TATCAAC 21205 0.0 28.009207 2 AACGCAG 21635 0.0 27.433273 6 GTACATG 18485 0.0 25.516535 1 TAACGCA 560 0.0 25.175224 4 TACATGG 19040 0.0 24.155033 2 ACGCAGA 24415 0.0 24.039639 7 CATGGGG 11175 0.0 23.843811 4 ACATGGG 18840 0.0 23.546764 3 CGCAGAG 24950 0.0 23.48649 8 CCTGGGG 5880 0.0 23.177193 4 ATGGGGG 6855 0.0 22.005829 5 >>END_MODULE