FastQCFastQC Report
Mon 6 Jun 2016
ERR1211520_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211520_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8001880
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT242340.30285382935010274No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT211250.2640004598919254No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT135980.16993506525966398No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA120500.048.9188541
GTATCAA209700.037.7198371
TCAACGC248750.030.999254
ATCAACG250400.030.9087373
CAACGCA251600.030.5528145
TACCTGG71500.029.9231342
TATCAAC264000.029.4066032
AACGCAG262600.029.356956
GTACATG242700.028.7693711
GTACCTG79250.028.6950381
TACATGG246000.027.3721942
ACATGGG246900.026.1699923
ACGCAGA295000.025.9259647
CGCAGAG298900.025.3997158
CATGGGG143850.024.597494
ACCTGGG88300.024.214343
TATAACG7800.023.5110342
GCAGAGT332700.022.087629
GAGTACT210100.020.73157112-13
ATGGGGG97100.020.3239735
Produced by FastQC (version 0.11.5)