FastQCFastQC Report
Mon 6 Jun 2016
ERR1211520_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211520_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8001880
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246780.30840252540652946No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220930.27609761705999086No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT152630.19074267547126425No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA131250.044.6384741
GTATCAA217700.035.659831
TACCTGG65400.029.836282
TCAACGC260350.028.9143354
GTACCTG70000.028.8842471
ATCAACG262400.028.5811753
CAACGCA265450.028.3942185
AACGCAG275950.027.364216
TATCAAC280350.026.9519142
GTACATG250300.026.3001251
TACATGG260150.025.1407172
ACATGGG258050.024.1280943
ACGCAGA313450.023.8504527
CGCAGAG318800.023.3175498
ACCTGGG81800.023.0357363
CATGGGG146200.022.6594854
ATGGGGG91350.021.2446615
GCAGAGT358500.020.4598799
GAGTACT213500.020.21548312-13
TAACGCA7600.019.7853934
Produced by FastQC (version 0.11.5)