##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211520_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 8001880 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1208949646833 33.0 31.0 34.0 30.0 34.0 2 32.34391093093123 34.0 31.0 34.0 31.0 34.0 3 32.362165516103715 34.0 31.0 34.0 30.0 34.0 4 35.99509052872575 37.0 35.0 37.0 35.0 37.0 5 35.910213974715944 37.0 35.0 37.0 35.0 37.0 6 35.92552150244693 37.0 35.0 37.0 35.0 37.0 7 35.850591361030155 37.0 35.0 37.0 35.0 37.0 8 35.84199700570366 37.0 35.0 37.0 35.0 37.0 9 37.50111948692057 39.0 37.0 39.0 35.0 39.0 10-11 37.45907386764111 39.0 37.0 39.0 34.5 39.0 12-13 37.37335657370518 39.0 37.0 39.0 34.0 39.0 14-15 38.60985468414923 40.0 38.0 41.0 34.0 41.0 16-17 38.53694787974826 40.0 38.0 41.0 34.0 41.0 18-19 38.51899909771204 40.0 38.0 41.0 34.0 41.0 20-21 38.455363552065265 40.0 38.0 41.0 34.0 41.0 22-23 38.349748121691405 40.0 38.0 41.0 34.0 41.0 24-25 38.242582618084754 40.0 38.0 41.0 33.5 41.0 26-27 38.071409343804206 40.0 37.0 41.0 33.0 41.0 28-29 38.11511069898574 40.0 38.0 41.0 33.0 41.0 30-31 38.046402532904764 40.0 37.5 41.0 33.0 41.0 32-33 37.87452092508261 40.0 37.0 41.0 33.0 41.0 34-35 37.7672161417067 40.0 37.0 41.0 33.0 41.0 36-37 37.71624443255835 40.0 37.0 41.0 33.0 41.0 38-39 37.62436314965983 40.0 37.0 41.0 32.5 41.0 40-41 37.47022630931731 40.0 37.0 41.0 32.0 41.0 42-43 37.326402482916514 40.0 36.0 41.0 31.0 41.0 44-45 37.151960789214534 40.0 36.0 41.0 31.0 41.0 46-47 36.910984106235034 39.0 36.0 41.0 30.0 41.0 48-49 36.704365161687 39.0 35.5 41.0 30.0 41.0 50-51 36.51294433308172 39.0 35.0 41.0 29.5 41.0 52-53 36.320495371087794 39.0 35.0 40.0 29.0 41.0 54-55 36.12628788484706 39.0 35.0 40.0 28.0 41.0 56-57 35.930921483451385 38.5 35.0 40.0 28.0 41.0 58-59 35.67423855393983 38.0 34.0 40.0 28.0 41.0 60-61 35.361109701720096 38.0 34.0 40.0 27.0 41.0 62-63 35.03943260833704 37.5 34.0 40.0 26.0 41.0 64-65 34.725584800071985 37.0 33.5 40.0 26.0 41.0 66-67 34.38632683819303 36.5 33.0 39.0 26.0 41.0 68-69 33.9858793808455 36.0 32.5 39.0 25.5 40.0 70-71 33.59536002789345 35.5 32.0 39.0 25.0 40.0 72-73 33.18055619429434 35.0 32.0 38.0 24.0 40.0 74-75 32.763836685878815 35.0 31.0 37.0 23.0 39.5 76-77 31.214438919353952 33.5 29.5 35.5 21.5 38.5 78-79 32.034849247926736 34.0 31.0 36.0 22.5 39.0 80-81 31.99191165076207 34.5 31.0 36.0 23.0 38.0 82-83 31.698074702444927 34.0 31.0 35.5 22.0 37.0 84-85 31.388628609776703 34.0 30.5 35.0 21.0 37.0 86-87 31.08463192399786 34.0 30.0 35.0 20.0 36.0 88-89 30.799716254180268 34.0 30.0 35.0 20.0 36.0 90-91 30.507608899408638 34.0 29.5 35.0 19.5 35.5 92-93 30.285484786075273 34.0 29.0 35.0 19.0 35.0 94-95 30.062782183686835 34.0 29.0 35.0 18.0 35.0 96-97 29.854792873674686 34.0 29.0 35.0 18.0 35.0 98-99 29.684925729953463 34.0 29.0 35.0 17.5 35.0 100 27.805365114198164 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3426659510331973 1101 2 0.26428495986078104 1101 3 0.3238643957934926 1101 4 0.22139834915004997 1101 5 0.21193360031332986 1101 6 0.24515339810299253 1101 7 0.20829745673616884 1101 8 0.22901904226490188 1101 9 0.2546178921422424 1101 10-11 0.29158508893375057 1101 12-13 0.2659470891644631 1101 14-15 0.2923484055133301 1101 16-17 0.3201675099384076 1101 18-19 0.37135089570940494 1101 20-21 0.34446958003839967 1101 22-23 0.3130726478786343 1101 24-25 0.3162820933393178 1101 26-27 0.30283505346984185 1101 28-29 0.35943320322839867 1101 30-31 0.4255877373117798 1101 32-33 0.44228245486914375 1101 34-35 0.3819436651671708 1101 36-37 0.40633519889263425 1101 38-39 0.40636781273418165 1101 40-41 0.40771157832075033 1101 42-43 0.3925510055872721 1101 44-45 0.40082864179615996 1101 46-47 0.4308978930417027 1101 48-49 0.3804419018140237 1101 50-51 0.3732637511128658 1101 52-53 0.35189422534445214 1101 54-55 0.3893194832059592 1101 56-57 0.4170630622560765 1101 58-59 0.4151921239545615 1101 60-61 0.3500780278003006 1101 62-63 0.41465763216552887 1101 64-65 0.46036874734678435 1101 66-67 0.37086722377634374 1101 68-69 0.4178840941833215 1101 70-71 0.3853691681729714 1101 72-73 0.26067794232244523 1101 74-75 0.3685443430328874 1101 76-77 0.41172457117981764 1101 78-79 0.447776907528052 1101 80-81 0.4322874290309393 1101 82-83 0.42151841617844 1101 84-85 0.3448492693545404 1101 86-87 0.2793047419515027 1101 88-89 0.24983373178003276 1101 90-91 0.2410389727869422 1101 92-93 0.15749141309970938 1101 94-95 0.1872647852619167 1101 96-97 0.18992896179932472 1101 98-99 0.11278112637351967 1101 100 0.057718605364016184 1102 1 0.3034770051578093 1102 2 0.2072146728194042 1102 3 0.295561602623053 1102 4 0.19385645342005375 1102 5 0.22006670846309362 1102 6 0.18168396934932218 1102 7 0.20772223852353733 1102 8 0.21084324517833863 1102 9 0.2847868436895169 1102 10-11 0.24588512918222705 1102 12-13 0.2513513668210834 1102 14-15 0.3330322187360082 1102 16-17 0.33010305266821405 1102 18-19 0.3320582791361417 1102 20-21 0.3072816692640288 1102 22-23 0.30703415000127876 1102 24-25 0.3669733071284469 1102 26-27 0.31579151853160425 1102 28-29 0.3892581713177705 1102 30-31 0.4089378174501519 1102 32-33 0.4769992725316641 1102 34-35 0.4350541213951118 1102 36-37 0.4255008663351134 1102 38-39 0.4492975404039541 1102 40-41 0.41699471833916135 1102 42-43 0.34289071239135893 1102 44-45 0.35152560274110556 1102 46-47 0.38639079299339585 1102 48-49 0.36653857812029145 1102 50-51 0.364927586189701 1102 52-53 0.30577519397509434 1102 54-55 0.3880440387861128 1102 56-57 0.42092641857571067 1102 58-59 0.4894867093087285 1102 60-61 0.446489472079314 1102 62-63 0.40629213075798987 1102 64-65 0.4189486349518887 1102 66-67 0.39061921634947083 1102 68-69 0.40018724309388176 1102 70-71 0.3894359858226224 1102 72-73 0.4959918598068498 1102 74-75 0.45992659076734554 1102 76-77 0.3888783028045353 1102 78-79 0.3724974474792191 1102 80-81 0.40183062932117153 1102 82-83 0.3894699374970507 1102 84-85 0.3328132073738672 1102 86-87 0.32867809417248495 1102 88-89 0.2956555795364757 1102 90-91 0.2530256175639103 1102 92-93 0.24103587901466383 1102 94-95 0.2910057901754044 1102 96-97 0.3281004441056794 1102 98-99 0.24725200671311143 1102 100 0.3298803302306297 1103 1 0.3420141368827174 1103 2 0.29190926142816664 1103 3 0.3619107148841323 1103 4 0.21547644703128555 1103 5 0.20611467674410022 1103 6 0.2343947846530554 1103 7 0.2617597990591207 1103 8 0.2659815505555727 1103 9 0.28452500796001345 1103 10-11 0.27840076593684415 1103 12-13 0.3311370867672778 1103 14-15 0.4181311898700315 1103 16-17 0.4912364218352394 1103 18-19 0.3921303679142554 1103 20-21 0.37845043140287515 1103 22-23 0.4134304919830569 1103 24-25 0.4311142159695649 1103 26-27 0.41926294414775356 1103 28-29 0.44343165730332856 1103 30-31 0.45889887100222637 1103 32-33 0.5678274939910111 1103 34-35 0.5832369266138073 1103 36-37 0.6181207180216433 1103 38-39 0.598335620237826 1103 40-41 0.5614209723213435 1103 42-43 0.6766360248327103 1103 44-45 0.6032387974300732 1103 46-47 0.7195962532544726 1103 48-49 0.6709138426892522 1103 50-51 0.6543261919572458 1103 52-53 0.762463111075995 1103 54-55 0.8540996511182541 1103 56-57 0.8287918517889281 1103 58-59 0.8095370460728404 1103 60-61 0.8326169839730753 1103 62-63 0.8934383554865235 1103 64-65 0.8312262681406821 1103 66-67 0.8335842557299742 1103 68-69 0.8319043652571949 1103 70-71 0.8982270696255554 1103 72-73 0.8580100970010989 1103 74-75 0.7398633365275415 1103 76-77 0.8210571514272829 1103 78-79 0.8575079894624338 1103 80-81 0.8298182522951159 1103 82-83 0.8008185619959214 1103 84-85 0.761529042877271 1103 86-87 0.7658610418086234 1103 88-89 0.7664035517631866 1103 90-91 0.8351351896634718 1103 92-93 0.7401613809388543 1103 94-95 0.8250686598967221 1103 96-97 0.8944208166463667 1103 98-99 0.8060347131277794 1103 100 0.983027949864212 1104 1 0.26409979411910456 1104 2 0.21405330076105145 1104 3 0.2474275865119253 1104 4 0.07681715459037264 1104 5 0.1620837857706121 1104 6 0.20060953022121453 1104 7 0.19886712375330262 1104 8 0.18500101124163137 1104 9 0.2757256691027834 1104 10-11 0.26090832397298414 1104 12-13 0.274569700439109 1104 14-15 0.3624799951101849 1104 16-17 0.3122201604165582 1104 18-19 0.2970356949983497 1104 20-21 0.24746482196395903 1104 22-23 0.3259604544679604 1104 24-25 0.34725526818733243 1104 26-27 0.3772240421341735 1104 28-29 0.41275829503255324 1104 30-31 0.36686362042357956 1104 32-33 0.36829368626866454 1104 34-35 0.46709982399849537 1104 36-37 0.34401849886222635 1104 38-39 0.46437980248553146 1104 40-41 0.5132847932975224 1104 42-43 0.5826834804108287 1104 44-45 0.5293188205642991 1104 46-47 0.5376576244070463 1104 48-49 0.5368701325550305 1104 50-51 0.5500148158724656 1104 52-53 0.48773872418090747 1104 54-55 0.5331475297877901 1104 56-57 0.5934282223103651 1104 58-59 0.5831877553112434 1104 60-61 0.5827415662847315 1104 62-63 0.5039607464060154 1104 64-65 0.6044578061849677 1104 66-67 0.7055342109850429 1104 68-69 0.712220122502039 1104 70-71 0.6569021837167739 1104 72-73 0.7402769361528883 1104 74-75 0.7462722337240919 1104 76-77 0.678005927867126 1104 78-79 0.6637742425458271 1104 80-81 0.7099255923509951 1104 82-83 0.5626796887379406 1104 84-85 0.643126843889803 1104 86-87 0.6933511547456739 1104 88-89 0.7440133090511161 1104 90-91 0.6140214078991981 1104 92-93 0.5606893765140732 1104 94-95 0.6647314578300936 1104 96-97 0.6413759738713836 1104 98-99 0.6864471417771512 1104 100 0.858690212194201 1105 1 0.27731114834337944 1105 2 0.16163978843086824 1105 3 0.21437615635258567 1105 4 0.12409503018875512 1105 5 0.11167453069647593 1105 6 0.15794493414287558 1105 7 0.16691231026669584 1105 8 0.16417676485677646 1105 9 0.18319763497871122 1105 10-11 0.23160744117152632 1105 12-13 0.2555770779416022 1105 14-15 0.30346072781449607 1105 16-17 0.3085768885200437 1105 18-19 0.31870503975620323 1105 20-21 0.2933647317721224 1105 22-23 0.3839201503212948 1105 24-25 0.3831123912085488 1105 26-27 0.3994966856614468 1105 28-29 0.4238261123149911 1105 30-31 0.44139985014636096 1105 32-33 0.47634043452072916 1105 34-35 0.4822098014260803 1105 36-37 0.48583034617603005 1105 38-39 0.49782593627465843 1105 40-41 0.56216029149261 1105 42-43 0.521121165097675 1105 44-45 0.554602702835929 1105 46-47 0.5320972196675982 1105 48-49 0.5815751033670935 1105 50-51 0.6625771506359754 1105 52-53 0.6502755288768043 1105 54-55 0.5465750309289561 1105 56-57 0.6733192330367288 1105 58-59 0.739305439042063 1105 60-61 0.7154361282564139 1105 62-63 0.6739459520591424 1105 64-65 0.7110494359533561 1105 66-67 0.6831847326655875 1105 68-69 0.7421515592415489 1105 70-71 0.7634868196570821 1105 72-73 0.7113926467548168 1105 74-75 0.755192919833938 1105 76-77 0.6943819503722537 1105 78-79 0.8222992434246024 1105 80-81 0.7719190531606621 1105 82-83 0.8206797035560349 1105 84-85 0.7943889554256387 1105 86-87 0.7430142630849659 1105 88-89 0.7161486912413046 1105 90-91 0.7904160547479258 1105 92-93 0.8346711643521836 1105 94-95 0.9125914015828478 1105 96-97 0.8714058341436512 1105 98-99 0.9205539172381982 1105 100 1.0851110625422251 1106 1 0.22019584048712204 1106 2 0.19122284630150688 1106 3 0.21059990774710968 1106 4 0.15355647409150208 1106 5 0.1574209750039941 1106 6 0.12428456816653011 1106 7 0.16204253195927976 1106 8 0.16870157101103445 1106 9 0.17849535009482764 1106 10-11 0.18963737785043833 1106 12-13 0.2225974211242132 1106 14-15 0.25327277649079605 1106 16-17 0.3038121702576646 1106 18-19 0.2708678148200221 1106 20-21 0.3073020190319937 1106 22-23 0.30857703812051085 1106 24-25 0.33034593630979714 1106 26-27 0.35750853658077375 1106 28-29 0.3733838067516828 1106 30-31 0.330253143268159 1106 32-33 0.35179867276199417 1106 34-35 0.34739629901781655 1106 36-37 0.32899386034162603 1106 38-39 0.3520207866884064 1106 40-41 0.42195963449729135 1106 42-43 0.4158288797439056 1106 44-45 0.4599316690603459 1106 46-47 0.4991063609001287 1106 48-49 0.4962807258899673 1106 50-51 0.496810932900857 1106 52-53 0.5025820910875822 1106 54-55 0.36865532143882973 1106 56-57 0.4986122075279198 1106 58-59 0.5529227224570832 1106 60-61 0.6005896889629838 1106 62-63 0.5842371678378626 1106 64-65 0.6334106615372121 1106 66-67 0.6055701839636569 1106 68-69 0.5785771855361475 1106 70-71 0.6390927067867906 1106 72-73 0.66150113420575 1106 74-75 0.5841487870125732 1106 76-77 0.5700482095199177 1106 78-79 0.633749973875581 1106 80-81 0.6393269220382685 1106 82-83 0.6500910179441952 1106 84-85 0.6055209944943627 1106 86-87 0.5452220798221212 1106 88-89 0.6037286372013853 1106 90-91 0.5787414151547523 1106 92-93 0.6693273616271362 1106 94-95 0.7497905739068784 1106 96-97 0.711662508453994 1106 98-99 0.7277217582688991 1106 100 0.7891413073283502 1107 1 0.22366398999481873 1107 2 0.2039992217340867 1107 3 0.23552089467563064 1107 4 0.14287509422042888 1107 5 0.13397207875806316 1107 6 0.16369734660611357 1107 7 0.1786069108270354 1107 8 0.1544002428857567 1107 9 0.19823913928210146 1107 10-11 0.19388429826974374 1107 12-13 0.26119211578916435 1107 14-15 0.28158652831965725 1107 16-17 0.2347618400188125 1107 18-19 0.19565411610864913 1107 20-21 0.22317650227534358 1107 22-23 0.3043206549056876 1107 24-25 0.31966009513419635 1107 26-27 0.3292096748324198 1107 28-29 0.3657951849390244 1107 30-31 0.3089196997622068 1107 32-33 0.25746611703022637 1107 34-35 0.28706162923779743 1107 36-37 0.40318562797898494 1107 38-39 0.413015106735962 1107 40-41 0.4246167035887609 1107 42-43 0.4133902175653361 1107 44-45 0.4111452703602154 1107 46-47 0.3450249262808427 1107 48-49 0.422805792021812 1107 50-51 0.41601243717648373 1107 52-53 0.40625198307406407 1107 54-55 0.43580643239453565 1107 56-57 0.45344745390295316 1107 58-59 0.5394528810854382 1107 60-61 0.5154599211169497 1107 62-63 0.4166791109182526 1107 64-65 0.5800391920575976 1107 66-67 0.556974560050925 1107 68-69 0.5436266077025209 1107 70-71 0.6077874723254695 1107 72-73 0.598813253387803 1107 74-75 0.5652422550412055 1107 76-77 0.5321650230213351 1107 78-79 0.643548949654118 1107 80-81 0.6304959349507406 1107 82-83 0.7138884353674477 1107 84-85 0.5548646546164697 1107 86-87 0.47653862515210577 1107 88-89 0.47198640334434927 1107 90-91 0.44130474550155085 1107 92-93 0.47092244704389685 1107 94-95 0.6551667432881985 1107 96-97 0.6440204061495223 1107 98-99 0.814257771247302 1107 100 0.901379709172641 1108 1 0.2309649097133999 1108 2 0.19099355498357085 1108 3 0.2524186015577641 1108 4 0.15783478735453826 1108 5 0.12808111306790693 1108 6 0.14028101699691575 1108 7 0.14753626071849624 1108 8 0.13904316844914177 1108 9 0.14292691553594494 1108 10-11 0.19426765735195062 1108 12-13 0.23470611928553353 1108 14-15 0.2573013377938551 1108 16-17 0.3142564863440285 1108 18-19 0.3295519979814543 1108 20-21 0.33088896586652794 1108 22-23 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2316 76-77 -0.43855505503229963 2316 78-79 -0.2572888791942063 2316 80-81 -0.2808851911421648 2316 82-83 -0.21794038345219846 2316 84-85 -0.2134291018136949 2316 86-87 -0.14293753374621687 2316 88-89 -0.13690613661707474 2316 90-91 -0.14619280134285617 2316 92-93 -0.01905815475247863 2316 94-95 0.08714165666056317 2316 96-97 0.04384741025721084 2316 98-99 -0.04931792274224733 2316 100 -0.17169497381026488 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 4.0 10 16.0 11 33.0 12 99.0 13 263.0 14 775.0 15 1918.0 16 4522.0 17 9088.0 18 16097.0 19 24110.0 20 34561.0 21 48291.0 22 62170.0 23 71236.0 24 78841.0 25 87954.0 26 104269.0 27 125633.0 28 151306.0 29 181473.0 30 219351.0 31 262969.0 32 320877.0 33 399442.0 34 520854.0 35 699758.0 36 1021814.0 37 1607992.0 38 1670079.0 39 276060.0 40 24.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.80120780209862 20.172984494932415 13.784533224555659 24.241274478413306 2 14.78417480307792 21.373792543897604 40.10481251697604 23.737220136048435 3 17.096229196771787 26.77948512482718 30.96836808312306 25.155917595277977 4 11.807686400402307 17.674119487663287 38.493462821771026 32.02473129016338 5 12.306903442879637 38.36274290121887 35.88167654595033 13.448677109951172 6 30.56276744660066 38.27661443987008 18.059429148153715 13.101188965375549 7 26.563932475868167 32.9950711582778 22.96634041000365 17.474655955850373 8 25.27867219626621 36.69878658324362 20.53832988514438 17.4842113353458 9 26.27525095365137 15.257872029009198 20.452841308713108 38.014035708626324 10-11 24.14996850740076 26.615657820412203 29.548980989467477 19.68539268271956 12-13 24.727726484276193 24.422254520187757 29.263166905777144 21.586852089758906 14-15 22.11799602093508 25.958655965848028 26.724238553939824 25.199109459277068 16-17 21.762722069708364 28.801736804225737 27.416583695990276 22.018957430075623 18-19 21.57861333125713 27.997990699204312 29.227772016218623 21.195623953319938 20-21 22.325564392993797 27.29029188124541 29.173608743971148 21.210534981789642 22-23 22.07856291762454 27.198316345553568 28.94496224293305 21.778158493888842 24-25 21.838408203873115 27.283354322153585 29.10461405377412 21.773623420199183 26-27 21.810136422545913 27.40620578961171 29.031695292592048 21.751962495250325 28-29 21.820213745456883 27.472699137038724 29.129274854462572 21.577812263041817 30-31 22.185024882629047 27.334599970231576 29.079233374328446 21.40114177281093 32-33 21.920836539189153 27.446758215056967 29.00880801401364 21.623597231740245 34-35 22.141400462259973 27.550059625887858 28.960709901746633 21.347830010105532 36-37 22.104083747491803 27.501447180173184 28.87242544397688 21.522043628358134 38-39 22.032865904771544 27.612833805573338 28.86702077637319 21.487279513281926 40-41 22.099309969896126 27.427098483558478 28.98625095099802 21.487340595547376 42-43 21.96986597588073 27.48041854588761 28.800069388542216 21.74964608968944 44-45 21.942055308665985 27.710141692611472 28.6909525853501 21.656850413372442 46-47 21.9262577694236 27.554774573062495 28.516547431094942 22.002420226418963 48-49 21.884067199707843 27.671621072126033 28.56027577378906 21.88403595437707 50-51 21.97456233472804 27.84788318161037 28.400218667035944 21.777335816625644 52-53 21.997647460803616 27.862186244269406 28.26083940808094 21.87932688684604 54-55 21.909233509318664 27.780392150001465 28.400384742833356 21.90998959784651 56-57 22.01325335753328 27.845585493679103 28.16625603366197 21.974905115125647 58-59 21.967729895577975 27.811655307399448 28.358119887400253 21.862494909622324 60-61 22.136598700262184 27.925553653366087 28.25491863453291 21.682929011838816 62-63 21.97328554789087 27.91469388607062 28.347208884952597 21.764811681085916 64-65 21.85966803358719 27.727596953719974 28.330891622729936 22.0818433899629 66-67 21.881585837894182 27.763586620289537 28.214426320479635 22.140401221336646 68-69 21.762006697005795 27.862803745933736 28.284463139033676 22.090726418026797 70-71 21.813737620359525 27.67552956691317 28.289181302774853 22.22155150995245 72-73 21.75223646623681 27.912089724981374 28.050073647457918 22.285600161323895 74-75 21.772574327114288 27.9320368183786 27.982038967933637 22.31334988657348 76-77 21.64641545718877 27.771649189880804 28.1741780645071 22.40775728842333 78-79 21.66564849318657 27.660632280731924 28.188036978194425 22.48568224788708 80-81 21.64224399657485 27.892428729606024 27.933096554276705 22.53223071954242 82-83 21.7708572899897 27.88270446068775 27.953674492785506 22.392763756537043 84-85 21.692250051481334 27.89883347514787 27.891221424734603 22.51769504863619 86-87 21.476039644554 27.875961395434302 28.049184446674975 22.598814513336727 88-89 21.496451959265965 27.702154573828867 28.074351984140378 22.727041482764783 90-91 21.721018081999315 27.720096825665674 27.974261073044875 22.584624019290132 92-93 21.555554944377672 27.69587577442836 27.963761290921035 22.784807990272938 94-95 21.388106551663093 27.59414383914435 27.992633667149068 23.02511594204348 96-97 21.327904200598745 27.56278398260011 28.067112080549496 23.042199736251646 98-99 21.26465968262452 27.507268672717426 27.926639933933544 23.30143171072451 100 20.372006399680014 28.02593620318984 28.155392230388482 23.446665166741663 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 2765.0 1 2003.5 2 2142.5 3 2966.0 4 2301.0 5 1927.5 6 2811.5 7 5359.0 8 8455.0 9 10034.5 10 8733.0 11 6911.0 12 6971.5 13 7465.0 14 7974.0 15 8530.0 16 9157.0 17 9847.5 18 10561.0 19 11542.0 20 13127.5 21 15240.5 22 18346.5 23 22813.0 24 28474.5 25 35862.5 26 45078.0 27 56349.5 28 69307.5 29 83271.5 30 99486.0 31 116021.5 32 134100.0 33 154349.0 34 175840.0 35 195192.5 36 216759.0 37 239281.5 38 254215.5 39 265511.5 40 271052.0 41 274616.0 42 280149.5 43 286172.5 44 292715.5 45 300746.0 46 305441.5 47 306671.0 48 310023.5 49 310169.0 50 305916.5 51 294454.5 52 277536.5 53 263065.5 54 246341.0 55 224077.5 56 200139.5 57 176091.0 58 152194.5 59 127451.5 60 102888.0 61 81925.0 62 64518.5 63 49113.5 64 35903.5 65 26090.0 66 19104.0 67 14054.0 68 10345.0 69 7485.5 70 5542.5 71 4147.5 72 3021.0 73 2155.0 74 1555.5 75 1082.0 76 723.5 77 461.0 78 295.5 79 199.5 80 142.0 81 111.5 82 76.5 83 53.0 84 38.5 85 27.0 86 17.5 87 13.5 88 10.5 89 8.5 90 5.5 91 5.0 92 6.0 93 2.5 94 1.0 95 0.5 96 2.0 97 4.0 98 4.5 99 6.0 100 5.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002974301039255775 2 0.06643438791883907 3 7.12332601838568E-4 4 4.124030852749604E-4 5 0.0014871505196278874 6 6.123560963173654E-4 7 0.0 8 0.00118722100306428 9 4.998825276060126E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.12332601838568E-4 18-19 0.0011434812818987537 20-21 0.0031555084555129546 22-23 6.248531595075157E-6 24-25 1.2497063190150314E-5 26-27 1.6871035306702926E-4 28-29 9.060370812858978E-4 30-31 0.0014121681404869854 32-33 0.003080526076372053 34-35 0.004024054347228401 36-37 0.0013996710772968353 38-39 0.0015308902407934134 40-41 0.005217523881887756 42-43 0.006985858323294026 44-45 0.0054924592720710635 46-47 0.007029598044459552 48-49 0.008554239753657891 50-51 0.008423020590161312 52-53 0.003911580778517049 54-55 0.002143246337110779 56-57 0.0026181347383364907 58-59 0.0032929761506046078 60-61 0.007173314271146281 62-63 0.013959219583397901 64-65 0.003967817562872725 66-67 0.010122621184021754 68-69 0.012384589621438962 70-71 0.005448719550905537 72-73 0.00731703049783301 74-75 0.0027993421545936706 76-77 0.0027243597754527684 78-79 0.0077981674306537956 80-81 0.005854874104585422 82-83 0.016496123410998414 84-85 0.01758961644013657 86-87 0.022369743110369066 88-89 0.021120036791354033 90-91 0.02418181727294086 92-93 0.034416912025673964 94-95 0.03819102510909936 96-97 0.022869625637975077 98-99 0.018133238688908104 100 0.018495653521422465 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 8001880.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.583894323216995 #Duplication Level Percentage of deduplicated Percentage of total 1 74.48264522930431 46.61413997944444 2 16.0922356677374 20.14229552907961 3 4.9706353417841 9.332451508483913 4 1.856292173280761 4.646959728224718 5 0.8914227725334625 2.789435429677165 6 0.48885625936002003 1.835671708501839 7 0.2839457448846093 1.2439301343969866 8 0.19597756767092764 0.9812031507870753 9 0.12813997376299435 0.7217548718906733 >10 0.5594641718764488 5.8747966639273885 >50 0.029282534279550593 1.275108495584942 >100 0.019111877416234584 2.3040986691738694 >500 0.0014257725335414188 0.6214942480809535 >1k 4.842116362278181E-4 0.5722882045375702 >5k 0.0 0.0 >10k+ 8.070193927821322E-5 1.0443716782088563 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24678 0.30840252540652946 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 22093 0.27609761705999086 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15263 0.19074267547126425 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.499412638030063E-5 2 0.0 0.0 0.0 0.0 2.499412638030063E-5 3 0.0 0.0 0.0 0.0 2.499412638030063E-5 4 0.0 0.0 0.0 2.499412638030063E-5 2.499412638030063E-5 5 0.0 2.499412638030063E-5 0.0 2.499412638030063E-5 2.499412638030063E-5 6 0.0 2.499412638030063E-5 0.0 2.499412638030063E-5 2.499412638030063E-5 7 0.0 2.499412638030063E-5 0.0 2.499412638030063E-5 2.499412638030063E-5 8 1.2497063190150314E-5 2.499412638030063E-5 0.0 4.998825276060126E-5 3.749118957045094E-5 9 1.2497063190150314E-5 2.499412638030063E-5 0.0 4.998825276060126E-5 3.749118957045094E-5 10-11 1.2497063190150314E-5 3.749118957045094E-5 0.0 4.998825276060126E-5 3.749118957045094E-5 12-13 1.2497063190150314E-5 3.749118957045094E-5 0.0 4.998825276060126E-5 4.3739721165526096E-5 14-15 1.2497063190150314E-5 3.749118957045094E-5 0.0 7.498237914090188E-5 4.998825276060126E-5 16-17 1.2497063190150314E-5 3.749118957045094E-5 0.0 7.498237914090188E-5 6.248531595075157E-5 18-19 1.2497063190150314E-5 5.623678435567642E-5 0.0 8.123091073597704E-5 7.498237914090188E-5 20-21 1.2497063190150314E-5 9.997650552120251E-5 0.0 1.4371622668672864E-4 8.123091073597704E-5 22-23 1.2497063190150314E-5 9.997650552120251E-5 0.0 2.0620154263748018E-4 9.997650552120253E-5 24-25 2.499412638030063E-5 9.997650552120251E-5 0.0 2.9992951656360753E-4 1.1247356871135284E-4 26-27 2.499412638030063E-5 9.997650552120251E-5 0.0 5.12379590796163E-4 1.1247356871135284E-4 28-29 2.499412638030063E-5 9.997650552120251E-5 0.0 0.0015746299619589397 1.1247356871135284E-4 30-31 2.499412638030063E-5 9.997650552120251E-5 0.0 0.005554944588021814 1.1247356871135284E-4 32-33 2.499412638030063E-5 9.997650552120251E-5 0.0 0.014871505196278874 1.2497063190150315E-4 34-35 2.499412638030063E-5 9.997650552120251E-5 0.0 0.02935560143366309 1.4996475828180376E-4 36-37 2.499412638030063E-5 1.0622503711627768E-4 0.0 0.05328747744280094 1.5621328987687895E-4 38-39 2.499412638030063E-5 1.1872210030642799E-4 0.0 0.08902907816663085 1.624618214719541E-4 40-41 2.499412638030063E-5 1.2497063190150315E-4 0.0 0.12893844946437588 1.624618214719541E-4 42-43 2.499412638030063E-5 1.2497063190150315E-4 0.0 0.16802301459157098 1.6871035306702926E-4 44-45 2.499412638030063E-5 1.2497063190150315E-4 0.0 0.21816123211045405 1.9370447944732987E-4 46-47 2.499412638030063E-5 1.3746769509165346E-4 0.0 0.27927811964188415 2.1245007423255534E-4 48-49 2.499412638030063E-5 1.3746769509165346E-4 0.0 0.34693721975335795 2.2494713742270567E-4 50-51 3.1242657975375787E-5 1.3746769509165346E-4 0.0 0.42018875564242403 2.2494713742270567E-4 52-53 3.749118957045094E-5 1.3746769509165346E-4 0.0 0.49349027978425075 2.4369273220793114E-4 54-55 3.749118957045094E-5 1.4996475828180376E-4 0.0 0.578151634365924 2.6868685858823173E-4 56-57 3.749118957045094E-5 1.749588846621044E-4 0.0 0.6767472144046149 2.749353901833069E-4 58-59 3.749118957045094E-5 1.749588846621044E-4 0.0 0.7896706773908131 2.811839217783821E-4 60-61 3.749118957045094E-5 1.749588846621044E-4 0.0 0.9141914150174709 2.874324533734572E-4 62-63 3.749118957045094E-5 1.749588846621044E-4 0.0 1.041661459557004 2.9992951656360753E-4 64-65 3.749118957045094E-5 1.749588846621044E-4 0.0 1.1733305173284279 3.061780481586827E-4 66-67 3.749118957045094E-5 1.749588846621044E-4 0.0 1.3282191185071508 3.124265797537579E-4 68-69 3.749118957045094E-5 1.749588846621044E-4 0.0 1.5076769459177095 3.124265797537579E-4 70-71 3.749118957045094E-5 1.8120741625717957E-4 0.0 1.708598479357351 3.124265797537579E-4 72-73 4.3739721165526096E-5 1.8745594785225472E-4 0.0 1.9071080796012936 3.6241483251435913E-4 74-75 4.998825276060126E-5 1.8745594785225472E-4 0.0 2.109666728318845 3.6241483251435913E-4 76-77 4.998825276060126E-5 1.8745594785225472E-4 0.0 2.333657840407504 3.686633641094343E-4 78-79 4.998825276060126E-5 1.8745594785225472E-4 0.0 2.591134833314171 3.7491189570450944E-4 80-81 6.248531595075157E-5 1.8745594785225472E-4 0.0 2.8673449239428734 3.9365749048973493E-4 82-83 6.248531595075157E-5 1.9370447944732987E-4 0.0 3.1591201067749077 4.873854644158623E-4 84-85 7.498237914090188E-5 1.9995301104240503E-4 0.0 3.455050563117667 4.873854644158623E-4 86-87 7.498237914090188E-5 2.1245007423255534E-4 0.0 3.7643716226686728 4.998825276060126E-4 88 7.498237914090188E-5 2.1245007423255534E-4 0.0 4.014981479352352 4.998825276060126E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 13125 0.0 44.638474 1 GTATCAA 21770 0.0 35.65983 1 TACCTGG 6540 0.0 29.83628 2 TCAACGC 26035 0.0 28.914335 4 GTACCTG 7000 0.0 28.884247 1 ATCAACG 26240 0.0 28.581175 3 CAACGCA 26545 0.0 28.394218 5 AACGCAG 27595 0.0 27.36421 6 TATCAAC 28035 0.0 26.951914 2 GTACATG 25030 0.0 26.300125 1 TACATGG 26015 0.0 25.140717 2 ACATGGG 25805 0.0 24.128094 3 ACGCAGA 31345 0.0 23.850452 7 CGCAGAG 31880 0.0 23.317549 8 ACCTGGG 8180 0.0 23.035736 3 CATGGGG 14620 0.0 22.659485 4 ATGGGGG 9135 0.0 21.244661 5 GCAGAGT 35850 0.0 20.459879 9 GAGTACT 21350 0.0 20.215483 12-13 TAACGCA 760 0.0 19.785393 4 >>END_MODULE