FastQCFastQC Report
Mon 6 Jun 2016
ERR1211519_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211519_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7072809
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT241760.34181610163656334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207010.2926842786225388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138170.19535378376540355No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT81740.11556935865226955No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA121350.047.573461
TACCTGG71200.041.8050542
GTACCTG79400.038.7856671
GTATCAA193800.034.3808561
ACCTGGG85850.034.3216483
GTACATG219800.029.5855771
TACATGG223650.028.0473632
TCAACGC233150.027.93514
CAACGCA234200.027.7868465
ATCAACG236150.027.581393
ACATGGG223150.026.9137883
AACGCAG243350.026.8151176
TATCAAC246650.026.4042432
CATGGGG135350.025.5182864
ACGCAGA269350.023.9135847
CGCAGAG274950.023.3244978
ATGGGGG89300.022.6778665
CCTGGGG82400.022.2983114
TAACGCA7400.020.3207954
TATAACG7200.020.2457712
Produced by FastQC (version 0.11.5)