FastQCFastQC Report
Mon 6 Jun 2016
ERR1211519_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211519_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7072809
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT246730.3488430127266267No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT217040.3068653486896083No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156540.22132649135583896No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT78200.1105642750991862No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA124750.044.1783681
TACCTGG73350.042.6969342
GTACCTG83000.038.2426761
ACCTGGG88350.034.7326133
GTATCAA198750.034.709281
TCAACGC243100.027.7394454
ATCAACG245450.027.4355683
CAACGCA248050.027.1858845
TATCAAC256450.026.4964542
AACGCAG256350.026.3238186
TAACGCA8400.025.7341234
TATAACG8250.025.6496332
GTACATG226000.025.426431
CCTGGGG80800.023.9034884
TACATGG235150.023.717142
ACATGGG227400.023.5996993
CATGGGG135450.023.3489254
ACGCAGA294300.022.7537177
ATGGGGG86850.022.617125
ATAACGC9650.022.4006863
Produced by FastQC (version 0.11.5)