##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211519_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 7072809 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08344195354349 33.0 31.0 34.0 30.0 34.0 2 32.30380941999141 34.0 31.0 34.0 30.0 34.0 3 32.318052417363454 34.0 31.0 34.0 30.0 34.0 4 35.96597886356043 37.0 35.0 37.0 35.0 37.0 5 35.880754732667036 37.0 35.0 37.0 35.0 37.0 6 35.898458448404305 37.0 35.0 37.0 35.0 37.0 7 35.82148068751751 37.0 35.0 37.0 35.0 37.0 8 35.81085336815966 37.0 35.0 37.0 35.0 37.0 9 37.46012425897546 39.0 37.0 39.0 34.0 39.0 10-11 37.41640294542098 39.0 37.0 39.0 34.0 39.0 12-13 37.32716774905133 39.0 37.0 39.0 34.0 39.0 14-15 38.548045691605694 40.0 38.0 41.0 34.0 41.0 16-17 38.471021202467085 40.0 38.0 41.0 34.0 41.0 18-19 38.45522054957232 40.0 38.0 41.0 34.0 41.0 20-21 38.38969891594698 40.0 38.0 41.0 34.0 41.0 22-23 38.278865582260174 40.0 38.0 41.0 34.0 41.0 24-25 38.168882759311046 40.0 37.5 41.0 33.0 41.0 26-27 37.99128005577416 40.0 37.0 41.0 33.0 41.0 28-29 38.02765365217695 40.0 37.0 41.0 33.0 41.0 30-31 37.9512271574137 40.0 37.0 41.0 33.0 41.0 32-33 37.76244975652531 40.0 37.0 41.0 33.0 41.0 34-35 37.644813326642925 40.0 37.0 41.0 33.0 41.0 36-37 37.58783278326899 40.0 37.0 41.0 32.0 41.0 38-39 37.49233741502138 40.0 37.0 41.0 32.0 41.0 40-41 37.33389103254449 40.0 36.0 41.0 31.0 41.0 42-43 37.18788595874709 40.0 36.0 41.0 31.0 41.0 44-45 37.01499771872816 39.0 36.0 41.0 30.5 41.0 46-47 36.76923956238603 39.0 35.5 41.0 30.0 41.0 48-49 36.5590987965319 39.0 35.0 41.0 30.0 41.0 50-51 36.36910688525592 39.0 35.0 40.5 29.0 41.0 52-53 36.173619703289035 39.0 35.0 40.0 28.5 41.0 54-55 35.977976571967375 38.5 35.0 40.0 28.0 41.0 56-57 35.78082498763928 38.0 34.5 40.0 28.0 41.0 58-59 35.52291904107689 38.0 34.0 40.0 27.0 41.0 60-61 35.202344146434605 38.0 34.0 40.0 26.0 41.0 62-63 34.874133388869964 37.0 33.5 40.0 26.0 41.0 64-65 34.5543964215632 37.0 33.0 40.0 26.0 41.0 66-67 34.20790699989212 36.0 33.0 39.0 25.5 41.0 68-69 33.814402806579395 36.0 32.0 39.0 25.0 40.0 70-71 33.421959365790876 35.0 32.0 38.5 24.0 40.0 72-73 33.00842974835034 35.0 31.0 38.0 23.5 40.0 74-75 32.59518834737372 35.0 31.0 37.0 22.5 39.0 76-77 31.05276284146794 33.5 29.5 35.5 21.0 38.5 78-79 31.881905901884245 34.0 30.5 36.0 22.0 39.0 80-81 31.847266835567027 34.0 31.0 36.0 22.0 37.5 82-83 31.550395889384262 34.0 31.0 35.5 21.0 37.0 84-85 31.239391732478566 34.0 30.0 35.0 20.0 37.0 86-87 30.9334839241382 34.0 30.0 35.0 20.0 36.0 88-89 30.651003300103255 34.0 29.5 35.0 19.5 36.0 90-91 30.352283300736666 34.0 29.0 35.0 19.0 35.5 92-93 30.131938950988214 34.0 29.0 35.0 18.0 35.0 94-95 29.901692029291333 34.0 29.0 35.0 18.0 35.0 96-97 29.68841304777211 34.0 29.0 35.0 17.5 35.0 98-99 29.51763245692058 33.5 29.0 35.0 17.0 35.0 100 27.655787679265764 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3214706823080533 1101 2 0.22811538523013297 1101 3 0.29143132726978394 1101 4 0.1659402832701815 1101 5 0.15098037848177626 1101 6 0.19450948701160087 1101 7 0.2227275526813841 1101 8 0.19153011358379501 1101 9 0.20956360579706512 1101 10-11 0.2081981055214115 1101 12-13 0.2534623500485367 1101 14-15 0.2882944079601799 1101 16-17 0.31775268316261673 1101 18-19 0.3248838395757403 1101 20-21 0.27030247427261855 1101 22-23 0.29833980398531423 1101 24-25 0.28109073995627654 1101 26-27 0.19609179864401227 1101 28-29 0.2511209923527815 1101 30-31 0.3913305937995517 1101 32-33 0.45678365443330904 1101 34-35 0.4059758371313009 1101 36-37 0.402887238059904 1101 38-39 0.3657631561928909 1101 40-41 0.3834650690321908 1101 42-43 0.3866247705287691 1101 44-45 0.3825676484109124 1101 46-47 0.40269529417945904 1101 48-49 0.4460839537085306 1101 50-51 0.3704479541242378 1101 52-53 0.3867855184707665 1101 54-55 0.446716770883846 1101 56-57 0.3442733198845289 1101 58-59 0.3485315846594972 1101 60-61 0.38692693237344145 1101 62-63 0.3593976762064486 1101 64-65 0.32245018544769266 1101 66-67 0.33197345065563866 1101 68-69 0.3821614320230964 1101 70-71 0.37992247632247 1101 72-73 0.23159217830344403 1101 74-75 0.22861866921841312 1101 76-77 0.2560557856298402 1101 78-79 0.32284992913253063 1101 80-81 0.26414408317067384 1101 82-83 0.22178374689919877 1101 84-85 0.21974973047298008 1101 86-87 0.22306417363116182 1101 88-89 0.03979002031259782 1101 90-91 0.04500491674921747 1101 92-93 -0.06126189351622813 1101 94-95 0.060286503957637905 1101 96-97 -0.03337189921870021 1101 98-99 -0.0332269813559698 1101 100 0.053422130478047336 1102 1 0.3183380324138838 1102 2 0.2478310804501831 1102 3 0.33556869340757345 1102 4 0.2094328705300228 1102 5 0.20492851147004387 1102 6 0.17737220721770797 1102 7 0.16640239557050052 1102 8 0.22365039296463607 1102 9 0.24346747003645675 1102 10-11 0.21477010116220896 1102 12-13 0.26034798405775916 1102 14-15 0.3236248133797801 1102 16-17 0.3736522465425409 1102 18-19 0.30376727298243367 1102 20-21 0.3487834751150558 1102 22-23 0.3250279438344208 1102 24-25 0.3210127154112712 1102 26-27 0.31704734553925107 1102 28-29 0.36137468005390616 1102 30-31 0.4254132132399917 1102 32-33 0.5349768033952031 1102 34-35 0.46965965278474187 1102 36-37 0.507467723405945 1102 38-39 0.5063639813605079 1102 40-41 0.46521103595548396 1102 42-43 0.4835873906226311 1102 44-45 0.545769396758125 1102 46-47 0.5102239568525277 1102 48-49 0.5006055735200192 1102 50-51 0.49036620745722814 1102 52-53 0.4830687637877773 1102 54-55 0.5245521760817482 1102 56-57 0.5054361854750837 1102 58-59 0.5160572369643006 1102 60-61 0.53517175932393 1102 62-63 0.598342448783626 1102 64-65 0.4817311624872147 1102 66-67 0.5104242311344507 1102 68-69 0.5327815912225731 1102 70-71 0.5355260784964742 1102 72-73 0.5747665766381687 1102 74-75 0.49220657313865956 1102 76-77 0.49408963010348117 1102 78-79 0.5418758066315448 1102 80-81 0.5399648169070979 1102 82-83 0.5032295521026953 1102 84-85 0.42585861946039927 1102 86-87 0.35037900681242107 1102 88-89 0.4166901110903716 1102 90-91 0.42193458614544 1102 92-93 0.30436751848183974 1102 94-95 0.26615012960784057 1102 96-97 0.2788500792700006 1102 98-99 0.3491872756548027 1102 100 0.34446710164618466 1103 1 0.2759158807442361 1103 2 0.23337762324389644 1103 3 0.2913888290377784 1103 4 0.12775568991790465 1103 5 0.14938815906353398 1103 6 0.17791009644237477 1103 7 0.18951290281207633 1103 8 0.22763646506886204 1103 9 0.2805432130306542 1103 10-11 0.24725926747517235 1103 12-13 0.28471289542974176 1103 14-15 0.3099170010146608 1103 16-17 0.35491663788531724 1103 18-19 0.3480742019438381 1103 20-21 0.3164401622445041 1103 22-23 0.31061278599822373 1103 24-25 0.3614708675718674 1103 26-27 0.33736142991992324 1103 28-29 0.3347122734386545 1103 30-31 0.4266108263071047 1103 32-33 0.5221277934869804 1103 34-35 0.48607907923049254 1103 36-37 0.4988501443907225 1103 38-39 0.5080276479239956 1103 40-41 0.5322576024878529 1103 42-43 0.5461609626003536 1103 44-45 0.491878404899019 1103 46-47 0.5860508428846671 1103 48-49 0.5497822533791279 1103 50-51 0.5615750690433927 1103 52-53 0.48448048315390224 1103 54-55 0.6607379609177784 1103 56-57 0.6662205749133463 1103 58-59 0.661763620323967 1103 60-61 0.7101962552987899 1103 62-63 0.6835226336081845 1103 64-65 0.6651347218036463 1103 66-67 0.7584776620414431 1103 68-69 0.6939786222003903 1103 70-71 0.6674251356544474 1103 72-73 0.6535891933815421 1103 74-75 0.6474532777966715 1103 76-77 0.7032656175004668 1103 78-79 0.6660131920670977 1103 80-81 0.6348946068856058 1103 82-83 0.6172676797666341 1103 84-85 0.5458650302536547 1103 86-87 0.6348378865506668 1103 88-89 0.6314460645495821 1103 90-91 0.5831086510855528 1103 92-93 0.5112348035487635 1103 94-95 0.6358208881517022 1103 96-97 0.5787909296243505 1103 98-99 0.5629254473388166 1103 100 0.7363007148510405 1104 1 0.2815962807422139 1104 2 0.25040658537353977 1104 3 0.2881754262537797 1104 4 0.08222177351022708 1104 5 0.13794169401750622 1104 6 0.16755660286801088 1104 7 0.19703179455941466 1104 8 0.23754564605525985 1104 9 0.2910134610380055 1104 10-11 0.24718763691202383 1104 12-13 0.2839409465727698 1104 14-15 0.36944293635593084 1104 16-17 0.35286325444652533 1104 18-19 0.3671582399828637 1104 20-21 0.2794321315496475 1104 22-23 0.3466618368164518 1104 24-25 0.3983310284128123 1104 26-27 0.4455007449338453 1104 28-29 0.48347718390930083 1104 30-31 0.39356166659792535 1104 32-33 0.41222763206694424 1104 34-35 0.535675211482129 1104 36-37 0.410546428511644 1104 38-39 0.4794256239478898 1104 40-41 0.5467787918811879 1104 42-43 0.6459202568286528 1104 44-45 0.503147785444888 1104 46-47 0.5906774846527512 1104 48-49 0.6108287043021434 1104 50-51 0.620300622021702 1104 52-53 0.6210215117396274 1104 54-55 0.6267720810207962 1104 56-57 0.656618876776875 1104 58-59 0.6686268229251411 1104 60-61 0.6414509118712459 1104 62-63 0.5370616182622641 1104 64-65 0.6794661201483692 1104 66-67 0.7205421834990204 1104 68-69 0.7306800553376149 1104 70-71 0.6159527305021939 1104 72-73 0.7943450465827908 1104 74-75 0.8351298578104078 1104 76-77 0.6392727315011726 1104 78-79 0.7196271453571015 1104 80-81 0.8118898487518997 1104 82-83 0.7068913404430504 1104 84-85 0.768172657145854 1104 86-87 0.7870510105717337 1104 88-89 0.7985246264828341 1104 90-91 0.7202542822435092 1104 92-93 0.7016620648860652 1104 94-95 0.6587702165715577 1104 96-97 0.6678911482030223 1104 98-99 0.6398903393016475 1104 100 0.923105047596593 1105 1 0.21404935296455818 1105 2 0.14825088606811931 1105 3 0.21739077149340602 1105 4 0.1594893056828397 1105 5 0.1010176394626825 1105 6 0.13416787567266653 1105 7 0.14140515567852674 1105 8 0.1501771979838935 1105 9 0.19770027599252415 1105 10-11 0.2143988159502257 1105 12-13 0.2615403390935995 1105 14-15 0.28688003194477574 1105 16-17 0.29696495478446394 1105 18-19 0.32905888562398644 1105 20-21 0.28257557905916286 1105 22-23 0.33111180518865524 1105 24-25 0.3647738127571074 1105 26-27 0.31757376012203764 1105 28-29 0.4493796194674573 1105 30-31 0.454009112221307 1105 32-33 0.487305417303844 1105 34-35 0.5004182983038561 1105 36-37 0.5111672070314413 1105 38-39 0.4836815182793188 1105 40-41 0.5122035617624121 1105 42-43 0.3971717234053145 1105 44-45 0.42614546737594594 1105 46-47 0.5467848944876437 1105 48-49 0.5611569098343807 1105 50-51 0.5762584927209957 1105 52-53 0.5069811034215164 1105 54-55 0.4054012703907759 1105 56-57 0.4107276399214115 1105 58-59 0.54033891760389 1105 60-61 0.5459090365220689 1105 62-63 0.5200598611137224 1105 64-65 0.4939665086732319 1105 66-67 0.5322572506142009 1105 68-69 0.5380666414206416 1105 70-71 0.573502140632904 1105 72-73 0.5484460635219577 1105 74-75 0.5231725009427421 1105 76-77 0.5184979998676909 1105 78-79 0.5951349740952239 1105 80-81 0.5791262216435591 1105 82-83 0.595812972338166 1105 84-85 0.5373467025402832 1105 86-87 0.5828423256248065 1105 88-89 0.5918198627550915 1105 90-91 0.7665307416290226 1105 92-93 0.6601137210816788 1105 94-95 0.6528044323420765 1105 96-97 0.5627975136523808 1105 98-99 0.6889765393297687 1105 100 0.7570245529375086 1106 1 0.27994922680559853 1106 2 0.18583445446405022 1106 3 0.22582561985834104 1106 4 0.1704777628176899 1106 5 0.1513666497261852 1106 6 0.11385482750349496 1106 7 0.19459059663640943 1106 8 0.15238009675402253 1106 9 0.15077302409707727 1106 10-11 0.19186076061208013 1106 12-13 0.25059489069337104 1106 14-15 0.30848992313934787 1106 16-17 0.29045600393266113 1106 18-19 0.30665506890164806 1106 20-21 0.3241594751020713 1106 22-23 0.3220914348462003 1106 24-25 0.34889589356142636 1106 26-27 0.3137782625240746 1106 28-29 0.333701126984181 1106 30-31 0.3770355578875666 1106 32-33 0.47967320434791105 1106 34-35 0.47833338488599253 1106 36-37 0.4454090989683621 1106 38-39 0.4666695911318399 1106 40-41 0.42316327375477414 1106 42-43 0.4145388295411081 1106 44-45 0.5091169196461678 1106 46-47 0.5580272983053831 1106 48-49 0.48805616068660385 1106 50-51 0.48221226171892795 1106 52-53 0.5478700252838635 1106 54-55 0.4548292381691752 1106 56-57 0.4895072147413302 1106 58-59 0.560918288766338 1106 60-61 0.5628877039193583 1106 62-63 0.5697639008698374 1106 64-65 0.5719007772692706 1106 66-67 0.5597815566740536 1106 68-69 0.4940270826437967 1106 70-71 0.5912854709576223 1106 72-73 0.6012379209133343 1106 74-75 0.5679855833431802 1106 76-77 0.5522865690972765 1106 78-79 0.6981225867010608 1106 80-81 0.6870445478622109 1106 82-83 0.6814803398278428 1106 84-85 0.5807217252569359 1106 86-87 0.5015802825877564 1106 88-89 0.5045478664949208 1106 90-91 0.6467920843612092 1106 92-93 0.7203327079401838 1106 94-95 0.6976090498530887 1106 96-97 0.7367917698954294 1106 98-99 0.8357985849836709 1106 100 0.8716800947907934 1107 1 0.27943070435321005 1107 2 0.21771410063434615 1107 3 0.271242254346447 1107 4 0.19437636585938378 1107 5 0.16248457041753284 1107 6 0.1871169977391247 1107 7 0.20777301268989135 1107 8 0.24257438904427175 1107 9 0.2711040163176719 1107 10-11 0.22370778059360674 1107 12-13 0.25803975116746614 1107 14-15 0.3708689917171455 1107 16-17 0.3290453022589688 1107 18-19 0.33510722541249294 1107 20-21 0.31210283570053576 1107 22-23 0.36316836393654484 1107 24-25 0.4469720750956654 1107 26-27 0.4576186745663975 1107 28-29 0.4376600223443674 1107 30-31 0.3827803008567088 1107 32-33 0.4233970489213661 1107 34-35 0.45908462606627864 1107 36-37 0.5040541369802796 1107 38-39 0.4887774952876356 1107 40-41 0.4943986423068836 1107 42-43 0.4883120476816174 1107 44-45 0.5272447074834758 1107 46-47 0.5412286201499725 1107 48-49 0.5841896946519896 1107 50-51 0.5314344206087824 1107 52-53 0.555575709460193 1107 54-55 0.637876945815826 1107 56-57 0.6419642091509488 1107 58-59 0.7350156100439165 1107 60-61 0.7265582932285639 1107 62-63 0.7499563735934061 1107 64-65 0.8129887433524985 1107 66-67 0.8371223366499265 1107 68-69 0.8444481944280966 1107 70-71 0.8705680116916241 1107 72-73 0.7212208863773171 1107 74-75 0.7861903024329635 1107 76-77 0.7028652477215971 1107 78-79 0.813219250421156 1107 80-81 0.8142965910234459 1107 82-83 0.7492362431663508 1107 84-85 0.7247539586814646 1107 86-87 0.7622638349413755 1107 88-89 0.7445756376905912 1107 90-91 0.5878542636777837 1107 92-93 0.6554036362441877 1107 94-95 0.8771218117181583 1107 96-97 0.9224845214322777 1107 98-99 0.9893377925242035 1107 100 1.07028277500644 1108 1 0.2850944193286651 1108 2 0.22509773723351856 1108 3 0.2723555136088436 1108 4 0.18328916579997667 1108 5 0.14912044689742032 1108 6 0.13635969045847673 1108 7 0.1341146272382403 1108 8 0.18373978590191342 1108 9 0.160249567294656 1108 10-11 0.19908274443380947 1108 12-13 0.2184640849633439 1108 14-15 0.26146916429822653 1108 16-17 0.30629011221721925 1108 18-19 0.33818938716191127 1108 20-21 0.3230063894205557 1108 22-23 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68-69 -0.06503751726037876 2316 70-71 -0.31212901318047415 2316 72-73 -0.40682310192366344 2316 74-75 -0.45909131893417765 2316 76-77 -0.39606876346786635 2316 78-79 -0.26927033321188887 2316 80-81 -0.20626564781158763 2316 82-83 -0.0955486565625634 2316 84-85 -0.025519142572878906 2316 86-87 -0.10375991921234728 2316 88-89 -0.0284866543104485 2316 90-91 -0.08592382517323927 2316 92-93 0.10552594932815396 2316 94-95 0.19271656834087025 2316 96-97 0.1553617321477141 2316 98-99 -0.012821216361398768 2316 100 -0.14161299335490796 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 3.0 10 14.0 11 35.0 12 85.0 13 223.0 14 756.0 15 1883.0 16 4392.0 17 9222.0 18 16008.0 19 23983.0 20 33592.0 21 44425.0 22 57112.0 23 65741.0 24 72585.0 25 82275.0 26 95626.0 27 115094.0 28 138338.0 29 166569.0 30 199422.0 31 239891.0 32 291231.0 33 362722.0 34 473545.0 35 632301.0 36 907687.0 37 1404026.0 38 1407224.0 39 226766.0 40 32.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.33541108610833 19.989027994873133 13.473834383521066 24.201726535497475 2 14.982654065864043 21.51821100256118 40.01250950202929 23.486625429545484 3 17.120199119464186 27.24934427807954 31.097994708980497 24.532461893475784 4 11.791171940369582 17.64558589949109 38.27458733966315 32.288654820476175 5 12.073661816044536 38.27884142140323 35.56743032117433 14.08006644137791 6 30.069197919453185 38.001339223352254 18.587294683995886 13.34216817319868 7 26.384297950078956 33.2208462012759 23.103946395272374 17.29090945337277 8 25.531943171169114 36.487458622871905 20.67976973023781 17.30082847572118 9 26.648709620682265 15.325859446974638 20.455437475716845 37.56999345662626 10-11 24.211398893989646 26.460809276766838 29.72016493022786 19.607626899015653 12-13 24.67786843954078 24.351265529720937 29.455821583758308 21.515044446979974 14-15 22.143140016929625 25.869042978539365 26.96100658168487 25.02681042284614 16-17 21.757382960573146 28.641687104834883 27.632801671145003 21.96812826344697 18-19 21.528546527077857 27.877082179988466 29.429800435489827 21.164570857443852 20-21 22.318064158318407 27.2499189652436 29.300230856267852 21.131786020170143 22-23 22.02951472321676 27.056866656515112 29.209992804838926 21.703625815429202 24-25 21.71407581585165 27.095919534651618 29.31241528736428 21.877589362132454 26-27 21.684422710486917 27.217943154927404 29.23499353792266 21.86264059666302 28-29 21.794420169321896 27.52242535656082 29.217439941782118 21.465714532335163 30-31 22.233235607896354 27.24042769200164 29.280820574132406 21.245516125969598 32-33 21.97351954645334 27.277160506307922 29.270928580427064 21.47839136681167 34-35 22.07626145374258 27.530714103570798 29.10983199846109 21.283192444225538 36-37 22.063894695043228 27.52436458104387 29.030709633379146 21.381031090533757 38-39 22.051048615177013 27.606883020353308 28.951226467821595 21.390841896648087 40-41 22.180641239645237 27.355439846145806 29.088802184205797 21.375116730003157 42-43 21.958448309102376 27.51513699847016 28.81812462339993 21.708290069027527 44-45 21.783723890131075 27.72756608728904 28.901071065164093 21.58763895741579 46-47 21.730678600924932 27.499451734308256 28.65598480607655 22.113884858690263 48-49 21.762977694650836 27.565740844498855 28.76645222319984 21.904829237650468 50-51 21.8462983552126 27.808279746914017 28.644339592894507 21.701082304978875 52-53 21.870102979961516 27.822143413519978 28.408439060303785 21.899314546214722 54-55 21.731776032340854 27.71619717831923 28.714456108759016 21.837570680580896 56-57 21.907113294430236 27.8124812657721 28.324413484345822 21.955991955451847 58-59 21.823647752198582 27.80732425775 28.58395887829404 21.78506911175738 60-61 22.16416533935987 27.886507863059972 28.445231618070473 21.504095179509687 62-63 22.011814221410738 27.95069894352455 28.468960448065957 21.56852638699876 64-65 21.909530586915523 27.629693968243913 28.452609771830012 22.00816567301055 66-67 21.800277117553243 27.69053104393907 28.42225260456988 22.08693923393781 68-69 21.602927407232276 27.723299043039706 28.543790101801864 22.129983447926154 70-71 21.719437179881634 27.62307589633142 28.49515806834018 22.162328855446763 72-73 21.559051677818186 28.056425933362366 28.097480586175887 22.28704180264356 74-75 21.66409771349146 27.963673307769405 28.03340018526438 22.338828793474757 76-77 21.54498408864717 27.801064753486788 28.247928668048615 22.406022489817428 78-79 21.48988314510305 27.717152964074636 28.334553480813874 22.45841041000844 80-81 21.554538814759756 27.883779725935277 28.055306130658554 22.506375328646417 82-83 21.758793753115874 27.919750310164375 28.051819846659626 22.269636090060125 84-85 21.665197166398105 28.041112540152003 27.885864020199207 22.407826273250688 86-87 21.458659789754112 27.973554404830924 28.013137522058678 22.554648283356286 88-89 21.346724462750686 27.7849342511092 28.147434883949167 22.72090640219094 90-91 21.757415578801897 27.77975178178316 28.02560543645653 22.437227202958418 92-93 21.481238370046736 27.87199603076188 27.980915296636695 22.665850302554695 94-95 21.35510348523558 27.747559135683186 27.96039794974328 22.93693942933795 96-97 21.310641267078427 27.74026116914689 27.98908864303751 22.96000892073717 98-99 21.192526617496217 27.635784566083956 27.889867013805443 23.281821802614385 100 20.22906700560619 28.332547075091057 28.163770238372006 23.27461568093075 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4089.0 1 3051.5 2 4037.5 3 5642.5 4 3825.0 5 2793.0 6 3975.5 7 7110.0 8 10652.5 9 11964.5 10 9927.5 11 7557.5 12 7433.5 13 7767.0 14 8330.0 15 8785.5 16 9227.5 17 9834.5 18 10461.0 19 11463.0 20 12806.0 21 14689.5 22 17597.0 23 22020.0 24 27698.5 25 34811.5 26 43786.5 27 53774.5 28 64581.5 29 77031.0 30 91406.5 31 105027.0 32 118703.5 33 134831.5 34 151782.5 35 167256.5 36 184398.0 37 200617.5 38 212737.5 39 223120.5 40 229462.5 41 233386.5 42 237923.5 43 244460.5 44 250380.5 45 256622.0 46 262769.0 47 266118.0 48 270514.5 49 274955.0 50 277056.5 51 269649.5 52 253564.5 53 239210.0 54 223318.5 55 202216.0 56 180001.5 57 159480.0 58 138783.0 59 116018.0 60 92929.5 61 73041.0 62 56632.5 63 42864.0 64 31871.0 65 22995.0 66 16539.0 67 12310.0 68 9110.5 69 6653.5 70 4930.5 71 3586.0 72 2640.0 73 1948.5 74 1395.0 75 960.0 76 616.0 77 393.5 78 271.0 79 184.5 80 126.0 81 83.5 82 65.5 83 47.0 84 35.0 85 29.5 86 22.0 87 17.0 88 14.5 89 9.0 90 4.0 91 6.0 92 7.0 93 3.5 94 3.5 95 3.0 96 0.5 97 2.0 98 2.0 99 3.5 100 3.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.00373260468365539 2 0.06478331310798863 3 9.897057873328688E-4 4 6.079621265044766E-4 5 0.0015269746433135688 6 7.493486675520292E-4 7 0.0 8 8.624579003900714E-4 9 7.069327052377635E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.564179946044068E-4 18-19 0.0011805776177470648 20-21 0.003202405154727068 22-23 0.0 24-25 7.069327052377633E-6 26-27 8.48319246285316E-5 28-29 9.543591520709806E-4 30-31 0.0014492120457374148 32-33 0.0027711762045320326 34-35 0.004022447092802874 36-37 0.0013219641587946176 38-39 0.0014138654104755269 40-41 0.005634253660744974 42-43 0.006864316567858683 44-45 0.005139400767078539 46-47 0.007182436285215676 48-49 0.008857866796629175 50-51 0.008313528613596098 52-53 0.0037891593000744118 54-55 0.0020501048451895135 56-57 0.0027287602422177665 58-59 0.0034286236204031525 60-61 0.00750055600257267 62-63 0.01403968352602198 64-65 0.0038881298788076986 66-67 0.010179830955423793 68-69 0.012519778209760787 70-71 0.006008927994520989 72-73 0.007479348021415536 74-75 0.0030186026513652495 76-77 0.0026297896634844796 78-79 0.008150934091391412 80-81 0.0060584132838876325 82-83 0.01633721481804471 84-85 0.017588485706315556 86-87 0.02279857974391787 88-89 0.02136350635228521 90-91 0.0244952182364885 92-93 0.0344205534180267 94-95 0.03775020645969656 96-97 0.023251016675270037 98-99 0.018175239851662896 100 0.018959935154476814 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 7072809.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.01769546650831 #Duplication Level Percentage of deduplicated Percentage of total 1 74.1224416035058 45.96903010600269 2 16.523649900934924 20.495173751027647 3 4.824159473964536 8.975497594146105 4 1.850475813369744 4.590489818468162 5 0.8859998434546503 2.747383423737227 6 0.4943790497829172 1.8396149612675228 7 0.2974981211232422 1.2915103514375745 8 0.19808034517549886 0.9827589219995949 9 0.13975347409026698 0.7800469556865063 >10 0.6117369217456291 6.4679960539552175 >50 0.03235848096781473 1.3823840805502856 >100 0.017610026456998176 2.0574672436713413 >500 0.0012969193682200566 0.5158784737529417 >1k 4.1265078095258415E-4 0.5342805427173143 >5k 5.89501115566453E-5 0.23657652878617813 >10k+ 8.842516733496795E-5 1.1339111927936498 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 24673 0.3488430127266267 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 21704 0.3068653486896083 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 15654 0.22132649135583896 No Hit GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 7820 0.1105642750991862 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 1.4138654104755268E-5 2 0.0 0.0 0.0 0.0 1.4138654104755268E-5 3 0.0 0.0 0.0 0.0 1.4138654104755268E-5 4 0.0 0.0 0.0 0.0 1.4138654104755268E-5 5 0.0 2.8277308209510536E-5 0.0 0.0 1.4138654104755268E-5 6 0.0 2.8277308209510536E-5 0.0 0.0 1.4138654104755268E-5 7 0.0 2.8277308209510536E-5 0.0 0.0 1.4138654104755268E-5 8 0.0 2.8277308209510536E-5 0.0 0.0 2.8277308209510536E-5 9 0.0 2.8277308209510536E-5 0.0 2.8277308209510536E-5 2.8277308209510536E-5 10-11 0.0 2.8277308209510536E-5 0.0 2.8277308209510536E-5 2.8277308209510536E-5 12-13 0.0 2.8277308209510536E-5 0.0 3.534663526188817E-5 2.8277308209510536E-5 14-15 0.0 4.24159623142658E-5 0.0 7.069327052377634E-5 4.24159623142658E-5 16-17 0.0 5.655461641902107E-5 0.0 8.48319246285316E-5 8.48319246285316E-5 18-19 0.0 7.069327052377634E-5 0.0 1.1310923283804215E-4 9.897057873328688E-5 20-21 0.0 7.069327052377634E-5 0.0 1.1310923283804215E-4 1.2017855989041978E-4 22-23 0.0 7.069327052377634E-5 0.0 1.7673317630944083E-4 1.272478869427974E-4 24-25 0.0 7.069327052377634E-5 0.0 3.605356796712593E-4 1.696638492570632E-4 26-27 1.4138654104755268E-5 7.069327052377634E-5 0.0 6.857247240806305E-4 1.696638492570632E-4 28-29 1.4138654104755268E-5 7.069327052377634E-5 0.0 0.0020359661910847584 1.696638492570632E-4 30-31 1.4138654104755268E-5 7.069327052377634E-5 0.0 0.007033980417115746 1.696638492570632E-4 32-33 1.4138654104755268E-5 7.069327052377634E-5 0.0 0.01717846473727765 1.696638492570632E-4 34-35 1.4138654104755268E-5 1.2017855989041978E-4 0.0 0.032780469541875086 1.696638492570632E-4 36-37 2.8277308209510536E-5 1.696638492570632E-4 0.0 0.05798262048360135 1.696638492570632E-4 38-39 4.24159623142658E-5 1.9794115746657375E-4 0.0 0.09787483304016834 1.696638492570632E-4 40-41 4.24159623142658E-5 2.0501048451895137E-4 0.0 0.14611592084559333 1.696638492570632E-4 42-43 4.24159623142658E-5 2.1207981157132902E-4 0.0 0.19285124198886183 1.696638492570632E-4 44-45 4.24159623142658E-5 2.544957738855948E-4 0.0 0.24852219252633573 1.696638492570632E-4 46-47 4.24159623142658E-5 2.544957738855948E-4 0.0 0.31550406634761374 1.696638492570632E-4 48-49 4.24159623142658E-5 2.544957738855948E-4 0.0 0.3896966537623171 1.696638492570632E-4 50-51 4.24159623142658E-5 2.544957738855948E-4 0.0 0.4697638519575461 1.7673317630944083E-4 52-53 4.24159623142658E-5 2.544957738855948E-4 0.0 0.5538747052267352 2.0501048451895137E-4 54-55 4.948528936664344E-5 2.686344279903501E-4 0.0 0.6510920908510325 2.262184656760843E-4 56-57 5.655461641902107E-5 2.686344279903501E-4 0.0 0.761811891145371 2.262184656760843E-4 58-59 5.655461641902107E-5 2.686344279903501E-4 0.0 0.8935558700934805 2.544957738855948E-4 60-61 5.655461641902107E-5 2.7570375504272775E-4 0.0 1.031775352621568 2.9691173619986064E-4 62-63 5.655461641902107E-5 2.8277308209510534E-4 0.0 1.1771843407619236 3.251890444093712E-4 64-65 5.655461641902107E-5 2.9691173619986064E-4 0.0 1.3264602507999297 3.3225837146174883E-4 66-67 5.655461641902107E-5 3.393276985141264E-4 0.0 1.5005494987917813 3.393276985141264E-4 68-69 6.36239434713987E-5 3.393276985141264E-4 0.0 1.6984835869313026 3.393276985141264E-4 70-71 7.069327052377634E-5 3.6760500672363696E-4 0.0 1.9156108980180293 3.6760500672363696E-4 72-73 7.069327052377634E-5 4.029516419855251E-4 0.0 2.1242691552960076 4.1002096903790274E-4 74-75 7.069327052377634E-5 4.1002096903790274E-4 0.0 2.345411844148485 4.1002096903790274E-4 76-77 8.48319246285316E-5 4.2415962314265804E-4 0.0 2.5963658851808384 4.1002096903790274E-4 78-79 8.48319246285316E-5 4.2415962314265804E-4 0.0 2.882327233776566 4.170902960902804E-4 80-81 9.190125168090924E-5 4.2415962314265804E-4 0.0 3.1841592216048813 4.382982772474133E-4 82-83 9.897057873328688E-5 4.312289501950357E-4 0.0 3.4997269118959666 4.7364491250930147E-4 84-85 9.897057873328688E-5 4.382982772474133E-4 0.0 3.8190130682166026 4.807142395616791E-4 86-87 1.1310923283804215E-4 4.382982772474133E-4 0.0 4.152133897578741 4.807142395616791E-4 88 1.1310923283804215E-4 4.382982772474133E-4 0.0 4.427689196753369 5.089915477711896E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 12475 0.0 44.178368 1 TACCTGG 7335 0.0 42.696934 2 GTACCTG 8300 0.0 38.242676 1 ACCTGGG 8835 0.0 34.732613 3 GTATCAA 19875 0.0 34.70928 1 TCAACGC 24310 0.0 27.739445 4 ATCAACG 24545 0.0 27.435568 3 CAACGCA 24805 0.0 27.185884 5 TATCAAC 25645 0.0 26.496454 2 AACGCAG 25635 0.0 26.323818 6 TAACGCA 840 0.0 25.734123 4 TATAACG 825 0.0 25.649633 2 GTACATG 22600 0.0 25.42643 1 CCTGGGG 8080 0.0 23.903488 4 TACATGG 23515 0.0 23.71714 2 ACATGGG 22740 0.0 23.599699 3 CATGGGG 13545 0.0 23.348925 4 ACGCAGA 29430 0.0 22.753717 7 ATGGGGG 8685 0.0 22.61712 5 ATAACGC 965 0.0 22.400686 3 >>END_MODULE