FastQCFastQC Report
Mon 6 Jun 2016
ERR1211518_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211518_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15237345
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT623030.4088835686269491No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT516860.3392060755991283No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT354850.23288177828880297No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA290750.054.9722751
GTATCAA535250.039.5216141
ATCAACG641150.031.8316963
TCAACGC646900.031.6204664
CAACGCA650600.031.3942095
AACGCAG674850.030.3916956
GTACATG708250.029.9144481
TATCAAC695750.029.548352
TACATGG732900.028.3007932
ACATGGG716200.027.8136943
ACGCAGA756550.026.8315937
CGCAGAG759000.026.5849298
CATGGGA415150.023.747464
CATGGGG251650.023.6590024
GCAGAGT837000.023.4417749
GAGTACT472950.022.07996612-13
TACCTGG109550.021.6763042
CAGAGTA824500.020.6200210-11
GTACCTG120900.020.6053961
AGAGTAC794000.020.60141610-11
Produced by FastQC (version 0.11.5)