FastQCFastQC Report
Mon 6 Jun 2016
ERR1211518_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211518_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15237345
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT670530.4400569784302974No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT573300.3762466492686226No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT414470.2720093297093424No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA304300.052.029671
GTATCAA542050.037.3546451
ATCAACG663800.029.881343
TCAACGC672600.029.5597674
CAACGCA677300.029.3474145
GTACATG707100.028.7750451
AACGCAG702200.028.4400336
TATCAAC716800.027.8663022
TACATGG729600.027.4547582
ACATGGG713000.027.0021513
ACGCAGA792400.024.953527
CGCAGAG793850.024.7954778
CATGGGG252200.023.3464494
CATGGGA412600.022.6071644
GCAGAGT885500.021.799159
GAGTACT505350.020.89371312-13
TACCTGG108300.020.8403782
AGAGTAC835300.020.46823910-11
GTACCTG116450.019.947911
AGTACTT563450.019.41060812-13
Produced by FastQC (version 0.11.5)