##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211518_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15237345 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.11734098033483 33.0 31.0 34.0 30.0 34.0 2 32.3337468568179 34.0 31.0 34.0 30.0 34.0 3 32.34503281247488 34.0 31.0 34.0 30.0 34.0 4 35.987528995372884 37.0 35.0 37.0 35.0 37.0 5 35.9157286915798 37.0 35.0 37.0 35.0 37.0 6 35.93471500448405 37.0 35.0 37.0 35.0 37.0 7 35.8635362000401 37.0 35.0 37.0 35.0 37.0 8 35.85626209815424 37.0 35.0 37.0 35.0 37.0 9 37.5093688565823 39.0 37.0 39.0 35.0 39.0 10-11 37.46929438822839 39.0 37.0 39.0 34.5 39.0 12-13 37.3936134214983 39.0 37.0 39.0 34.0 39.0 14-15 38.60872379669818 40.0 38.0 41.0 34.0 41.0 16-17 38.55468889101087 40.0 38.0 41.0 34.0 41.0 18-19 38.57531147978864 40.0 38.0 41.0 34.0 41.0 20-21 38.53456753784862 40.0 38.0 41.0 34.0 41.0 22-23 38.4288686775813 40.0 38.0 41.0 34.0 41.0 24-25 38.33029218672938 40.0 38.0 41.0 34.0 41.0 26-27 38.1622533321914 40.0 38.0 41.0 33.0 41.0 28-29 38.1988849763525 40.0 38.0 41.0 34.0 41.0 30-31 38.11700893429925 40.0 37.5 41.0 33.5 41.0 32-33 37.937280280783824 40.0 37.0 41.0 33.0 41.0 34-35 37.83283754486099 40.0 37.0 41.0 33.0 41.0 36-37 37.774717019270746 40.0 37.0 41.0 33.0 41.0 38-39 37.68975628628216 40.0 37.0 41.0 33.0 41.0 40-41 37.544463979781256 40.0 37.0 41.0 32.0 41.0 42-43 37.403841056299505 40.0 37.0 41.0 31.5 41.0 44-45 37.219921908967734 40.0 36.0 41.0 31.0 41.0 46-47 36.957564654472286 39.5 36.0 41.0 30.5 41.0 48-49 36.73183084060905 39.0 36.0 41.0 30.0 41.0 50-51 36.54652263895055 39.0 35.5 41.0 30.0 41.0 52-53 36.37430605528719 39.0 35.0 40.5 29.0 41.0 54-55 36.20066711096979 39.0 35.0 40.0 28.5 41.0 56-57 36.025185129036586 39.0 35.0 40.0 28.0 41.0 58-59 35.79298191384392 38.0 35.0 40.0 28.0 41.0 60-61 35.5145881713645 38.0 34.0 40.0 27.0 41.0 62-63 35.21878768906262 38.0 34.0 40.0 26.0 41.0 64-65 34.931561371091874 37.5 34.0 40.0 26.0 41.0 66-67 34.61611179637923 37.0 33.5 40.0 26.0 41.0 68-69 34.23349691169951 36.5 33.0 39.0 25.5 40.5 70-71 33.85926803521217 36.0 32.5 39.0 25.0 40.0 72-73 33.452884803750266 35.5 32.0 38.5 24.0 40.0 74-75 33.032066839728316 35.0 31.5 38.0 23.5 40.0 76-77 31.449719127577673 34.0 29.5 36.0 21.5 38.5 78-79 32.268206403412144 34.5 31.0 36.5 22.5 39.0 80-81 32.20745395605337 35.0 31.0 36.0 23.0 38.5 82-83 31.89245216276195 34.5 31.0 36.0 22.0 37.5 84-85 31.55975732648962 34.0 31.0 35.5 21.5 37.0 86-87 31.245975299502636 34.0 30.5 35.0 20.0 36.5 88-89 30.94230458127712 34.0 30.0 35.0 20.0 36.0 90-91 30.646730614815112 34.0 30.0 35.0 19.5 36.0 92-93 30.4225509759082 34.0 29.0 35.0 19.0 35.5 94-95 30.195456065344718 34.0 29.0 35.0 18.0 35.0 96-97 29.988567004291102 34.0 29.0 35.0 18.0 35.0 98-99 29.827349548100408 34.0 29.0 35.0 18.0 35.0 100 27.962837226564076 32.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3225153879502969 1101 2 0.23913256397568716 1101 3 0.3113882905569767 1101 4 0.18077451194083238 1101 5 0.2011653448661832 1101 6 0.21124559951353916 1101 7 0.24719582552731367 1101 8 0.22324291793947282 1101 9 0.28905921344494345 1101 10-11 0.2599471852388575 1101 12-13 0.24691066891009683 1101 14-15 0.35126577838580175 1101 16-17 0.38180988915412684 1101 18-19 0.40045146339635096 1101 20-21 0.3577951726712172 1101 22-23 0.3414500279195636 1101 24-25 0.31178496172618253 1101 26-27 0.29402324898585164 1101 28-29 0.37861778452694494 1101 30-31 0.410243779699762 1101 32-33 0.4599281133452777 1101 34-35 0.4331918756634465 1101 36-37 0.4423020412978218 1101 38-39 0.43942532895804476 1101 40-41 0.45549292511984163 1101 42-43 0.4430758512734627 1101 44-45 0.4603316184819377 1101 46-47 0.5132152427195535 1101 48-49 0.4867031417031811 1101 50-51 0.488476631522758 1101 52-53 0.5321910298459187 1101 54-55 0.485264854466088 1101 56-57 0.46693969369624 1101 58-59 0.5221782828109056 1101 60-61 0.484790762592894 1101 62-63 0.5153913357341438 1101 64-65 0.5633836834591648 1101 66-67 0.5020303199630618 1101 68-69 0.46088470116389857 1101 70-71 0.491974297736661 1101 72-73 0.39579768613669586 1101 74-75 0.4496736756184063 1101 76-77 0.50070621779183 1101 78-79 0.5924011626625614 1101 80-81 0.5724745768497073 1101 82-83 0.48291526034637755 1101 84-85 0.5193837329135711 1101 86-87 0.44210293155382274 1101 88-89 0.41577708106363076 1101 90-91 0.3965252589128916 1101 92-93 0.3778904286777589 1101 94-95 0.4184693808837139 1101 96-97 0.3756079608342908 1101 98-99 0.2904849436037118 1101 100 0.4878008913424239 1102 1 0.3240276452183082 1102 2 0.22825558047274797 1102 3 0.32400675280243263 1102 4 0.19969729857136542 1102 5 0.201308354162947 1102 6 0.18321145043144327 1102 7 0.1809634921157226 1102 8 0.19867185296776313 1102 9 0.22673845953376315 1102 10-11 0.2248542446880677 1102 12-13 0.27734012315023193 1102 14-15 0.35728576565909265 1102 16-17 0.35891872409222003 1102 18-19 0.3668795644060836 1102 20-21 0.35623194005229664 1102 22-23 0.36605983944883747 1102 24-25 0.33747064071475563 1102 26-27 0.32484105846486955 1102 28-29 0.40220667934752896 1102 30-31 0.4105631543248123 1102 32-33 0.493727117462349 1102 34-35 0.4648481339038142 1102 36-37 0.47871268008348977 1102 38-39 0.4378242376329098 1102 40-41 0.43330948448605255 1102 42-43 0.3862808504056332 1102 44-45 0.4373918843274609 1102 46-47 0.46440761530097063 1102 48-49 0.4960796872326796 1102 50-51 0.49716001882205774 1102 52-53 0.5146677832250361 1102 54-55 0.5167911475767326 1102 56-57 0.5187254137089852 1102 58-59 0.5662364183024167 1102 60-61 0.5518722642118945 1102 62-63 0.613786696627507 1102 64-65 0.5632279452731908 1102 66-67 0.6284693353837554 1102 68-69 0.5881929466085509 1102 70-71 0.5866465899019389 1102 72-73 0.6102698678423977 1102 74-75 0.5849939514125779 1102 76-77 0.621385054798612 1102 78-79 0.6114734670838118 1102 80-81 0.6243366937529515 1102 82-83 0.5799787862370955 1102 84-85 0.5430242481362448 1102 86-87 0.4856123114949291 1102 88-89 0.46608714865104517 1102 90-91 0.47875985174308866 1102 92-93 0.4467379499752724 1102 94-95 0.5263633686787266 1102 96-97 0.5658394869172163 1102 98-99 0.5221607436334246 1102 100 0.7209983308733605 1103 1 0.28811808384276816 1103 2 0.23800137462720983 1103 3 0.3095945342700972 1103 4 0.13672486318960608 1103 5 0.15216124016700405 1103 6 0.15242530747550376 1103 7 0.17610964841508547 1103 8 0.22030919693960982 1103 9 0.246387311277374 1103 10-11 0.2074858668751176 1103 12-13 0.2205632399546147 1103 14-15 0.36158477078158313 1103 16-17 0.3769059247513127 1103 18-19 0.3571800351451273 1103 20-21 0.35569525665809465 1103 22-23 0.3646054476465963 1103 24-25 0.36025260632018075 1103 26-27 0.3442570604078625 1103 28-29 0.413956941866914 1103 30-31 0.4420507325915466 1103 32-33 0.4772293115450381 1103 34-35 0.4579941693032552 1103 36-37 0.4877077871444939 1103 38-39 0.4862143665573555 1103 40-41 0.4830955057661157 1103 42-43 0.5162433528290293 1103 44-45 0.47070188484798337 1103 46-47 0.5928483933620043 1103 48-49 0.5519042417426832 1103 50-51 0.5379789364556871 1103 52-53 0.5061679789194287 1103 54-55 0.5972776752556896 1103 56-57 0.6152239313775496 1103 58-59 0.626450961129855 1103 60-61 0.6076257020601972 1103 62-63 0.6032587680074002 1103 64-65 0.5906537316272988 1103 66-67 0.6131003184615622 1103 68-69 0.643155767651173 1103 70-71 0.627700244072237 1103 72-73 0.5933246610060152 1103 74-75 0.5325788617888421 1103 76-77 0.6152617300567833 1103 78-79 0.6211314296897257 1103 80-81 0.589734236529587 1103 82-83 0.5598866365609503 1103 84-85 0.5383813149507155 1103 86-87 0.601882446303442 1103 88-89 0.6476778442170641 1103 90-91 0.6495166326219106 1103 92-93 0.5721059578391454 1103 94-95 0.6232565808965731 1103 96-97 0.6347343282545701 1103 98-99 0.6510838405170638 1103 100 0.6753073133748977 1104 1 0.26658247597896434 1104 2 0.22980571603003597 1104 3 0.2627086430182146 1104 4 0.05950084439583492 1104 5 0.15400188989827512 1104 6 0.1516541294897209 1104 7 0.19791603371593425 1104 8 0.21291121130264656 1104 9 0.25378367477303954 1104 10-11 0.250431126335414 1104 12-13 0.2716256343577186 1104 14-15 0.36414526073073006 1104 16-17 0.3047637141476329 1104 18-19 0.32273752966018066 1104 20-21 0.21557222865621384 1104 22-23 0.3079151241519753 1104 24-25 0.33987818126413316 1104 26-27 0.4066172783900015 1104 28-29 0.4392755033808555 1104 30-31 0.4030431309971263 1104 32-33 0.39046887429744714 1104 34-35 0.46904443609743396 1104 36-37 0.4147788980937932 1104 38-39 0.44786424286007076 1104 40-41 0.547685927706695 1104 42-43 0.590150483197398 1104 44-45 0.5095160611132243 1104 46-47 0.4933065641657919 1104 48-49 0.5329075853964369 1104 50-51 0.5572994503068003 1104 52-53 0.5476698029872651 1104 54-55 0.5537214993612594 1104 56-57 0.6530493524463736 1104 58-59 0.6360291347021061 1104 60-61 0.6237923526856903 1104 62-63 0.662506337168594 1104 64-65 0.7523038061917262 1104 66-67 0.7358806434016927 1104 68-69 0.7254189683666468 1104 70-71 0.6650934846455314 1104 72-73 0.7056560228969602 1104 74-75 0.7528599716651243 1104 76-77 0.6176711806279194 1104 78-79 0.7448455973395909 1104 80-81 0.8339197912292136 1104 82-83 0.7395607736088188 1104 84-85 0.7327938672963121 1104 86-87 0.7834812840331971 1104 88-89 0.7939414880775075 1104 90-91 0.7100111478017297 1104 92-93 0.6583591454969842 1104 94-95 0.7138717966059218 1104 96-97 0.7142194918499207 1104 98-99 0.7675368531518636 1104 100 0.8996912691719068 1105 1 0.2537137942483909 1105 2 0.14959956073525404 1105 3 0.21356312397266208 1105 4 0.10405237426462577 1105 5 0.09223887103723172 1105 6 0.11914996079768514 1105 7 0.1386186757261001 1105 8 0.1366693409995534 1105 9 0.15449401757304315 1105 10-11 0.17659246915333426 1105 12-13 0.18772033531695342 1105 14-15 0.24220655734878704 1105 16-17 0.2916358384054405 1105 18-19 0.24495070488509185 1105 20-21 0.20011926400771074 1105 22-23 0.23666443509374346 1105 24-25 0.26817573543024764 1105 26-27 0.262660276196911 1105 28-29 0.33150818409959015 1105 30-31 0.3838765398867494 1105 32-33 0.40304066393711224 1105 34-35 0.4219550361133457 1105 36-37 0.42313554704627876 1105 38-39 0.43820451154542894 1105 40-41 0.48789370893727124 1105 42-43 0.40895920353028714 1105 44-45 0.4562740628603521 1105 46-47 0.4501971112985643 1105 48-49 0.497429157982495 1105 50-51 0.5628122064893688 1105 52-53 0.5482108937431249 1105 54-55 0.4738917799650295 1105 56-57 0.4963794365847889 1105 58-59 0.5350263048511081 1105 60-61 0.5965743932174803 1105 62-63 0.6123338394187599 1105 64-65 0.6409881964237201 1105 66-67 0.5939503646585962 1105 68-69 0.5881537373137675 1105 70-71 0.6670911817216236 1105 72-73 0.6257023400563071 1105 74-75 0.6340847809704186 1105 76-77 0.6041952895246929 1105 78-79 0.6458143741671165 1105 80-81 0.6361749752205696 1105 82-83 0.7013565807650544 1105 84-85 0.7108712010128322 1105 86-87 0.6826876039577421 1105 88-89 0.6868810328765704 1105 90-91 0.7845544715629451 1105 92-93 0.7232917031456125 1105 94-95 0.8139542319379771 1105 96-97 0.8419073028850654 1105 98-99 0.8192088891540017 1105 100 1.0126988903354928 1106 1 0.26152681722659565 1106 2 0.2189596576871935 1106 3 0.22578726943568483 1106 4 0.15988772050764055 1106 5 0.16319977122565632 1106 6 0.15844703020988504 1106 7 0.17707548313822485 1106 8 0.15564791041721548 1106 9 0.19549282076403784 1106 10-11 0.20355361463083455 1106 12-13 0.24797670520621296 1106 14-15 0.32958903342847634 1106 16-17 0.3623933050038417 1106 18-19 0.3292230500507074 1106 20-21 0.31599322532436247 1106 22-23 0.3207959312958053 1106 24-25 0.34001144195293165 1106 26-27 0.35553075620064334 1106 28-29 0.3785647405710506 1106 30-31 0.43620533513033166 1106 32-33 0.45053919599335757 1106 34-35 0.45545158293140275 1106 36-37 0.4830393147945955 1106 38-39 0.4505110493989335 1106 40-41 0.45107473209452564 1106 42-43 0.4570219494337664 1106 44-45 0.5530257165211765 1106 46-47 0.5749429358433105 1106 48-49 0.5635059344066562 1106 50-51 0.5640883263571581 1106 52-53 0.6063036957202925 1106 54-55 0.5525374469073725 1106 56-57 0.6179247193170809 1106 58-59 0.7062426111117546 1106 60-61 0.6745324309382354 1106 62-63 0.6615164340657174 1106 64-65 0.7556621257316323 1106 66-67 0.7483347491105192 1106 68-69 0.7080055006582029 1106 70-71 0.716680866637617 1106 72-73 0.6954889686674974 1106 74-75 0.6727657093964652 1106 76-77 0.6719045750293837 1106 78-79 0.7623036965652901 1106 80-81 0.7692823840860825 1106 82-83 0.7968781833435834 1106 84-85 0.7716761032249444 1106 86-87 0.7225850874884223 1106 88-89 0.8179678394184648 1106 90-91 0.8190295846790399 1106 92-93 0.8025976010782969 1106 94-95 0.8623231810977359 1106 96-97 0.8387426675193232 1106 98-99 0.8862084632535208 1106 100 1.0606277556326482 1107 1 0.19397642307404084 1107 2 0.1619554586489329 1107 3 0.22775434513111037 1107 4 0.1463754487170874 1107 5 0.129438540263493 1107 6 0.13894422678259843 1107 7 0.15989281542416478 1107 8 0.16159482659626434 1107 9 0.1875109337117422 1107 10-11 0.17667843825816476 1107 12-13 0.19834657076299322 1107 14-15 0.24864414813851 1107 16-17 0.24038720749810238 1107 18-19 0.1892832887857665 1107 20-21 0.20088405625940453 1107 22-23 0.289326745567557 1107 24-25 0.30476422772704126 1107 26-27 0.30910459894583653 1107 28-29 0.29870968329707637 1107 30-31 0.22239599510689345 1107 32-33 0.27372231605090036 1107 34-35 0.3529285802248694 1107 36-37 0.34947818052203417 1107 38-39 0.37175479679802237 1107 40-41 0.3798007314511196 1107 42-43 0.3503084085494521 1107 44-45 0.4206722218403627 1107 46-47 0.435731626208522 1107 48-49 0.46101986787010674 1107 50-51 0.4191616055283589 1107 52-53 0.3846775627379273 1107 54-55 0.42531745776376084 1107 56-57 0.4719464817897574 1107 58-59 0.5642173513989661 1107 60-61 0.5391686456796663 1107 62-63 0.5700537042546543 1107 64-65 0.7013323629674915 1107 66-67 0.6722669771138996 1107 68-69 0.6082130406063229 1107 70-71 0.6881586855998876 1107 72-73 0.6047083993120665 1107 74-75 0.6455730358474838 1107 76-77 0.5756154923287831 1107 78-79 0.6788332638183903 1107 80-81 0.6357025488993315 1107 82-83 0.6587657966154445 1107 84-85 0.5476262273538275 1107 86-87 0.5140241432990713 1107 88-89 0.5286660792770022 1107 90-91 0.43355399411050044 1107 92-93 0.5162505926552257 1107 94-95 0.714570883282704 1107 96-97 0.6832548523263355 1107 98-99 0.7472823293725845 1107 100 0.8759080923589693 1108 1 0.24493016088185726 1108 2 0.19658996321484778 1108 3 0.2277721740699974 1108 4 0.1470060259832877 1108 5 0.12323336684248432 1108 6 0.12018632238366678 1108 7 0.15033381173581972 1108 8 0.16036763775199603 1108 9 0.13201051175062162 1108 10-11 0.1671571252262538 1108 12-13 0.20263310226772546 1108 14-15 0.2349760078399541 1108 16-17 0.24087818808063588 1108 18-19 0.2734429215542278 1108 20-21 0.24500978550620545 1108 22-23 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54-55 -0.32705479237993984 2316 56-57 -0.7284298141574723 2316 58-59 -0.645961201872332 2316 60-61 -0.4273327209185709 2316 62-63 -0.4248041386067243 2316 64-65 -0.4602060525785632 2316 66-67 -0.19563444767805294 2316 68-69 -0.2746391827634014 2316 70-71 -0.4313073050037488 2316 72-73 -0.47360560420612074 2316 74-75 -0.5519208696481925 2316 76-77 -0.48528173375595784 2316 78-79 -0.36150011242079927 2316 80-81 -0.32424288289573155 2316 82-83 -0.1821251611835173 2316 84-85 -0.23865855840269745 2316 86-87 -0.2771416997917413 2316 88-89 -0.2805052039200788 2316 90-91 -0.1348730819133266 2316 92-93 -0.09247963071033283 2316 94-95 -0.14661513511216384 2316 96-97 -0.0943024054965349 2316 98-99 -0.28377828849782816 2316 100 -0.4595952724597794 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 5.0 10 22.0 11 69.0 12 171.0 13 418.0 14 1200.0 15 3521.0 16 8366.0 17 17987.0 18 33251.0 19 50977.0 20 73822.0 21 103599.0 22 133479.0 23 143948.0 24 145176.0 25 156643.0 26 181629.0 27 219046.0 28 265869.0 29 322305.0 30 390126.0 31 472458.0 32 577338.0 33 723071.0 34 941496.0 35 1275040.0 36 1862221.0 37 3033883.0 38 3504652.0 39 595471.0 40 85.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.671511411937324 21.894890099345982 14.353811404087931 23.079787084628766 2 13.884588044487339 22.741345232910206 41.54307904966604 21.830987672936413 3 16.16257898352302 29.299680249589212 31.614659835401486 22.923080931486282 4 10.511564885150506 19.15914463807961 41.25701586756557 29.072274609204314 5 11.001741600601637 40.21539353892888 36.71997218899382 12.062892671475664 6 27.43896224311882 41.26721299888189 19.223890086721035 12.06993467127826 7 24.99054789400647 34.119041079663155 24.422719312321142 16.467691714009234 8 24.42456108227269 37.34357931364664 21.539981604225712 16.69187799985496 9 26.310322294463617 15.064060726088721 21.592425029068394 37.03319195037926 10-11 22.88197845490799 27.933156990276192 30.485872046606545 18.698992508209273 12-13 23.993897887066286 25.991470298795495 30.071416641153693 19.943215172984534 14-15 21.42858221035226 27.123232426646503 27.915050161297785 23.533135201703445 16-17 20.17196758571373 30.004320994784354 28.706824835376928 21.11688658412499 18-19 20.022962267733295 28.999887840072148 30.997384619494 19.979765272700554 20-21 21.177881563415863 28.19612801316702 30.898647749113834 19.727342674303284 22-23 20.772130628539987 28.387709826594453 30.404967558650643 20.43519198621492 24-25 20.448133276788038 28.56642582413652 30.78594350641995 20.19949739265549 26-27 20.417726988153856 28.633393909381905 30.716404501697003 20.23247460076724 28-29 20.293806739863438 28.325955443368077 31.090631325599144 20.289606491169337 30-31 20.63316895824416 28.418156549716606 30.858981583243324 20.08969290879591 32-33 20.443087095553576 28.571707030925563 30.765097329727652 20.2201085437932 34-35 20.66276742349525 28.65302844920502 30.819889520167322 19.864314607132407 36-37 20.737348934233456 28.363723411621134 30.574641394669804 20.324286259475606 38-39 20.438704952116936 28.83230558005712 30.689247705603158 20.039741762222782 40-41 20.547773573394064 28.49421631429521 30.964350816507597 19.993659295803138 42-43 20.449284909041275 28.40868588743636 30.623436031311712 20.518593172210657 44-45 20.493426725251833 28.764088489438933 30.429105882696543 20.31337890261269 46-47 20.581260347236938 28.757643734399668 30.08503698103999 20.576058937323406 48-49 20.461645424951033 28.876710187116956 30.113046887239737 20.548597500692274 50-51 20.67153831492261 28.893188049814494 29.767164687325742 20.668108947937156 52-53 20.70194057004245 28.989634279203713 29.68126425828063 20.627160892473203 54-55 20.70636009906814 29.029462024563585 29.711161737187226 20.553016139181054 56-57 20.721099778659344 29.095833373941932 29.52565447854112 20.657412368857607 58-59 20.571572377302978 28.958458858564924 29.842951712236626 20.627017051895475 60-61 20.83936434348114 29.304472520662898 29.45524845803751 20.400914677818452 62-63 20.568774180336142 29.06170327038508 29.874984677684953 20.49453787159383 64-65 20.48230947693736 28.855614473484685 29.752795079443384 20.90928097013457 66-67 20.539551242426203 29.007281162455108 29.49953773210043 20.953629863018268 68-69 20.508788815176224 29.12828076871312 29.54942281141596 20.8135076046947 70-71 20.58196531678095 28.7968936384564 29.67606024359309 20.94508080116956 72-73 20.54389827554414 29.02433716887404 29.392753984695403 21.03901057088641 74-75 20.482958817948717 29.147577141589164 29.23515419195224 21.134309848509883 76-77 20.320974802152957 28.99673076480073 29.56483528109431 21.117459151952 78-79 20.35028425723978 28.90322114011833 29.410988492700383 21.335506109941505 80-81 20.261034698903845 29.272068700464647 29.160392083341712 21.306504517289792 82-83 20.53580969724753 29.05582143882055 29.25992710352565 21.14844176040627 84-85 20.30825248804469 29.080650600926024 29.148922581372705 21.462174329656584 86-87 20.086011208541567 29.119272282735754 29.447584367908565 21.34713214081411 88-89 20.33528548662345 28.862056057711577 29.360686344866338 21.441972110798634 90-91 20.320489691385706 28.988112424812147 29.313820188662632 21.377577695139514 92-93 20.33683820212334 28.91561886991673 29.233082073948065 21.514460854011862 94-95 19.976748141290756 28.69237740374444 29.36327126130074 21.967603193664065 96-97 20.16342868609839 28.675424756940988 29.310026739551958 21.851119817408662 98-99 19.91151994903675 28.627449886384575 29.35283926966461 22.108190894914063 100 19.052833406303723 29.125832239871503 29.51633431772248 22.30500003610229 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 5670.0 1 3938.5 2 3162.0 3 4134.0 4 3502.0 5 3489.0 6 5531.5 7 11157.5 8 18081.0 9 21625.0 10 19382.0 11 16108.0 12 16603.5 13 17912.5 14 19158.0 15 20503.5 16 21969.0 17 23479.5 18 25585.5 19 28641.0 20 32784.5 21 38420.0 22 45888.0 23 55950.0 24 68247.5 25 84888.5 26 108734.5 27 135444.5 28 162100.0 29 198651.5 30 244630.5 31 287922.0 32 341871.5 33 406067.5 34 472524.5 35 529431.0 36 589124.0 37 639359.0 38 656746.0 39 668826.5 40 650796.5 41 619834.5 42 601344.5 43 591180.5 44 588141.0 45 589159.0 46 575060.5 47 546419.5 48 528808.5 49 508405.5 50 484132.5 51 441703.5 52 390345.0 53 350258.0 54 313557.5 55 274709.5 56 235472.0 57 198581.0 58 165111.0 59 136237.5 60 107102.0 61 81183.5 62 61377.5 63 45007.0 64 31792.5 65 21319.0 66 14150.0 67 9701.5 68 6753.0 69 4689.5 70 3248.5 71 2294.5 72 1624.5 73 1080.5 74 734.5 75 519.5 76 339.5 77 215.5 78 154.5 79 96.0 80 70.0 81 49.5 82 30.5 83 22.0 84 18.5 85 15.5 86 12.0 87 9.0 88 6.0 89 4.5 90 3.5 91 4.0 92 3.5 93 3.5 94 4.0 95 6.0 96 7.5 97 5.0 98 3.5 99 6.0 100 7.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003235471796431727 2 0.06799741030999823 3 9.712978212411677E-4 4 6.497194885329433E-4 5 0.0013913185006968077 6 6.300310191834601E-4 7 0.0 8 9.844234674741565E-4 9 3.937693869896626E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.415990121638644E-4 18-19 0.0011419312222700213 20-21 0.0032190647386404914 22-23 6.562823116494376E-6 24-25 1.968846934948313E-5 26-27 1.6078916635411223E-4 28-29 9.975491137071452E-4 30-31 0.0015947660173081333 32-33 0.0029762402833301994 34-35 0.004118171505600222 36-37 0.0012469363921339314 38-39 0.0014766352012112345 40-41 0.005689967642000624 42-43 0.0069992508537412524 44-45 0.00549636436006404 46-47 0.0071633214316536116 48-49 0.008964816377131318 50-51 0.008511981582093205 52-53 0.0038392515231492103 54-55 0.0023462092641467393 56-57 0.002785918412951863 58-59 0.003507828955766244 60-61 0.007616156226691723 62-63 0.014520246145243807 64-65 0.0040262919819693 66-67 0.010441451578342552 68-69 0.013037048120916078 70-71 0.0057096561113501075 72-73 0.00791148326693397 74-75 0.0031271852150095707 76-77 0.0029401447561894804 78-79 0.008095242314195812 80-81 0.006178897964179455 82-83 0.016991149048603937 84-85 0.017660557006486367 86-87 0.023078167489152476 88-89 0.02173935157338762 90-91 0.02461386809841216 92-93 0.0350684453229877 94-95 0.03855330439784621 96-97 0.023261926536414318 98-99 0.018622010593052792 100 0.018736859997591445 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.5237345E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 42.207894702701815 #Duplication Level Percentage of deduplicated Percentage of total 1 65.9600546808127 27.84035042552197 2 17.013805778725384 14.362338456013212 3 7.123808439478657 9.02042869487201 4 3.5205231545494167 5.943754824225818 5 1.9950085433457634 4.210255526426425 6 1.193403354731068 3.0222625880604013 7 0.7778035360966322 2.298061482566905 8 0.5355884851708436 1.808484990885643 9 0.38581881215607494 1.4656139818024594 >10 1.3685417750798976 9.018325733903083 >50 0.04962741636012648 1.4706469646358717 >100 0.054348045022584474 5.156984011632083 >500 0.011898908870873758 3.482392573642793 >1k 0.009093009420782552 6.983417898925942 >5k 5.74651152348798E-4 1.5506959492214916 >10k+ 1.01409026885082E-4 2.3659858976639336 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 67053 0.4400569784302974 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 57330 0.3762466492686226 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 41447 0.2720093297093424 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 6.562823116494376E-6 0.0 8 0.0 0.0 0.0 1.968846934948313E-5 0.0 9 0.0 0.0 0.0 7.219105428143814E-5 0.0 10-11 0.0 0.0 0.0 7.547246583968533E-5 0.0 12-13 0.0 0.0 0.0 8.53167005144269E-5 0.0 14-15 0.0 0.0 0.0 9.187952363092126E-5 0.0 16-17 0.0 0.0 0.0 9.516093518916846E-5 0.0 18-19 0.0 0.0 0.0 1.0172375830566283E-4 0.0 20-21 0.0 0.0 0.0 1.2797505077164032E-4 0.0 22-23 0.0 3.281411558247188E-6 0.0 2.1657316284431444E-4 0.0 24-25 0.0 6.562823116494376E-6 0.0 3.6751809452368505E-4 0.0 26-27 0.0 6.562823116494376E-6 0.0 6.136239613922242E-4 0.0 28-29 0.0 6.562823116494376E-6 0.0 0.0013880370891385606 0.0 30-31 0.0 6.562823116494376E-6 0.0 0.005033685330351187 0.0 32-33 3.281411558247188E-6 6.562823116494376E-6 0.0 0.013791772779312931 0.0 34-35 6.562823116494376E-6 6.562823116494376E-6 0.0 0.026966640185675392 0.0 36-37 6.562823116494376E-6 6.562823116494376E-6 0.0 0.04886349951385888 6.562823116494376E-6 38-39 6.562823116494376E-6 6.562823116494376E-6 0.0 0.08430930716604501 6.562823116494376E-6 40-41 6.562823116494376E-6 6.562823116494376E-6 0.0 0.1251202227159653 6.562823116494376E-6 42-43 6.562823116494376E-6 6.562823116494376E-6 0.0 0.165711283691483 6.562823116494376E-6 44-45 6.562823116494376E-6 6.562823116494376E-6 0.0 0.21630408709653814 6.562823116494376E-6 46-47 6.562823116494376E-6 6.562823116494376E-6 0.0 0.2787099721112832 9.844234674741565E-6 48-49 6.562823116494376E-6 6.562823116494376E-6 0.0 0.3472488153283922 1.3125646232988753E-5 50-51 6.562823116494376E-6 6.562823116494376E-6 0.0 0.4244374594130408 1.3125646232988753E-5 52-53 6.562823116494376E-6 6.562823116494376E-6 0.0 0.501209364229792 1.3125646232988753E-5 54-55 6.562823116494376E-6 6.562823116494376E-6 6.562823116494376E-6 0.5848066050876974 1.6407057791235943E-5 56-57 6.562823116494376E-6 6.562823116494376E-6 6.562823116494376E-6 0.6845155766965965 1.968846934948313E-5 58-59 6.562823116494376E-6 6.562823116494376E-6 6.562823116494376E-6 0.8028531217216648 1.968846934948313E-5 60-61 6.562823116494376E-6 6.562823116494376E-6 6.562823116494376E-6 0.9331218791725199 1.968846934948313E-5 62-63 6.562823116494376E-6 9.844234674741565E-6 6.562823116494376E-6 1.0660387357508805 1.968846934948313E-5 64-65 6.562823116494376E-6 1.3125646232988753E-5 6.562823116494376E-6 1.2009835046722377 1.968846934948313E-5 66-67 6.562823116494376E-6 1.3125646232988753E-5 6.562823116494376E-6 1.3559645725682525 1.968846934948313E-5 68-69 6.562823116494376E-6 1.3125646232988753E-5 6.562823116494376E-6 1.5377777427760546 1.968846934948313E-5 70-71 6.562823116494376E-6 1.3125646232988753E-5 6.562823116494376E-6 1.7459176779156738 1.968846934948313E-5 72-73 6.562823116494376E-6 1.3125646232988753E-5 6.562823116494376E-6 1.9613390653030434 1.968846934948313E-5 74-75 6.562823116494376E-6 1.3125646232988753E-5 6.562823116494376E-6 2.1737382726452674 1.968846934948313E-5 76-77 6.562823116494376E-6 1.3125646232988753E-5 6.562823116494376E-6 2.402908774461693 1.968846934948313E-5 78-79 6.562823116494376E-6 1.3125646232988753E-5 6.562823116494376E-6 2.6684832561053122 1.968846934948313E-5 80-81 6.562823116494376E-6 2.6251292465977505E-5 6.562823116494376E-6 2.9674001605922817 1.968846934948313E-5 82-83 6.562823116494376E-6 3.937693869896626E-5 6.562823116494376E-6 3.2836297924605633 1.968846934948313E-5 84-85 6.562823116494376E-6 3.937693869896626E-5 6.562823116494376E-6 3.593995541874257 2.2969880907730316E-5 86-87 6.562823116494376E-6 3.937693869896626E-5 6.562823116494376E-6 3.913979108565173 2.6251292465977505E-5 88 6.562823116494376E-6 3.937693869896626E-5 6.562823116494376E-6 4.17242636430428 2.6251292465977505E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 30430 0.0 52.02967 1 GTATCAA 54205 0.0 37.354645 1 ATCAACG 66380 0.0 29.88134 3 TCAACGC 67260 0.0 29.559767 4 CAACGCA 67730 0.0 29.347414 5 GTACATG 70710 0.0 28.775045 1 AACGCAG 70220 0.0 28.440033 6 TATCAAC 71680 0.0 27.866302 2 TACATGG 72960 0.0 27.454758 2 ACATGGG 71300 0.0 27.002151 3 ACGCAGA 79240 0.0 24.95352 7 CGCAGAG 79385 0.0 24.795477 8 CATGGGG 25220 0.0 23.346449 4 CATGGGA 41260 0.0 22.607164 4 GCAGAGT 88550 0.0 21.79915 9 GAGTACT 50535 0.0 20.893713 12-13 TACCTGG 10830 0.0 20.840378 2 AGAGTAC 83530 0.0 20.468239 10-11 GTACCTG 11645 0.0 19.94791 1 AGTACTT 56345 0.0 19.410608 12-13 >>END_MODULE