FastQCFastQC Report
Mon 6 Jun 2016
ERR1211517_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211517_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9527321
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT262480.2755024208799095No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT239070.2509309804928374No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT147690.1550173443300588No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA139750.045.820661
GTATCAA236950.036.5227171
TACCTGG85100.034.479312
GTACCTG95900.031.2748371
TCAACGC272750.030.9091764
CAACGCA277200.030.3431025
ATCAACG278200.030.3047753
AACGCAG288250.029.3196936
GTACATG280400.028.9322031
TATCAAC297000.028.5933342
TACATGG284200.027.581322
ACATGGG286800.026.2172163
ACCTGGG107900.026.1321753
ACGCAGA329000.025.4894477
CGCAGAG337100.024.6967058
CATGGGG166050.023.2345374
GCAGAGT379350.021.4280839
GAGTACT244250.019.3052812-13
ATGGGGG106950.019.112115
AGAGTAC349250.018.72039810-11
Produced by FastQC (version 0.11.5)