FastQCFastQC Report
Mon 6 Jun 2016
ERR1211517_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211517_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9527321
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT284450.29856241854347093No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT264640.2777695849651754No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT179630.18854198362792648No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA150000.046.1474881
GTATCAA250650.034.8210451
TACCTGG90800.033.7149162
GTACCTG100150.031.6475771
TCAACGC298950.028.6557544
ATCAACG300050.028.4413683
CAACGCA302850.028.3177665
GTACATG302500.027.4223521
AACGCAG316600.027.1618466
TATCAAC319700.026.8439962
ACCTGGG111250.026.7804283
TACATGG307900.026.4524042
ACATGGG309450.025.1325053
ACGCAGA364550.023.408617
CGCAGAG370750.022.8904048
CATGGGG174900.022.4615734
GCAGAGT420000.019.7918459
GAGTACT255750.019.42130912-13
ATGGGGG103500.018.7059845
AGAGTAC380250.018.65398610-11
Produced by FastQC (version 0.11.5)