##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211517_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9527321 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.1003127741786 33.0 31.0 34.0 30.0 34.0 2 32.32326516551715 34.0 31.0 34.0 30.0 34.0 3 32.341803850211406 34.0 31.0 34.0 30.0 34.0 4 35.98410056720037 37.0 35.0 37.0 35.0 37.0 5 35.903561137490804 37.0 35.0 37.0 35.0 37.0 6 35.91671016437884 37.0 35.0 37.0 35.0 37.0 7 35.83876989134721 37.0 35.0 37.0 35.0 37.0 8 35.832434427264495 37.0 35.0 37.0 35.0 37.0 9 37.48885820053717 39.0 37.0 39.0 35.0 39.0 10-11 37.44856560411894 39.0 37.0 39.0 34.5 39.0 12-13 37.361676960396316 39.0 37.0 39.0 34.0 39.0 14-15 38.600387926469566 40.0 38.0 41.0 34.0 41.0 16-17 38.527012787750095 40.0 38.0 41.0 34.0 41.0 18-19 38.50476707985382 40.0 38.0 41.0 34.0 41.0 20-21 38.44123689125201 40.0 38.0 41.0 34.0 41.0 22-23 38.331974959172676 40.0 38.0 41.0 34.0 41.0 24-25 38.22280413350195 40.0 38.0 41.0 33.5 41.0 26-27 38.047880091370914 40.0 37.0 41.0 33.0 41.0 28-29 38.08797341876064 40.0 38.0 41.0 33.0 41.0 30-31 38.02042226770779 40.0 37.0 41.0 33.0 41.0 32-33 37.848748772083994 40.0 37.0 41.0 33.0 41.0 34-35 37.73755082882166 40.0 37.0 41.0 33.0 41.0 36-37 37.6800953279521 40.0 37.0 41.0 33.0 41.0 38-39 37.58591029944304 40.0 37.0 41.0 32.0 41.0 40-41 37.426578520866464 40.0 36.5 41.0 31.5 41.0 42-43 37.27814492657485 40.0 36.0 41.0 31.0 41.0 44-45 37.09745787929262 39.5 36.0 41.0 31.0 41.0 46-47 36.853882586720864 39.0 36.0 41.0 30.0 41.0 48-49 36.64384715283551 39.0 35.0 41.0 30.0 41.0 50-51 36.451241067662146 39.0 35.0 40.5 29.5 41.0 52-53 36.252081881150005 39.0 35.0 40.0 29.0 41.0 54-55 36.055185870193725 39.0 35.0 40.0 28.0 41.0 56-57 35.85403861169368 38.0 34.5 40.0 28.0 41.0 58-59 35.59245432162935 38.0 34.0 40.0 27.5 41.0 60-61 35.27761859813478 38.0 34.0 40.0 26.5 41.0 62-63 34.95558111246593 37.5 34.0 40.0 26.0 41.0 64-65 34.64009420906465 37.0 33.0 40.0 26.0 41.0 66-67 34.2974556016324 36.0 33.0 39.0 26.0 41.0 68-69 33.89563031412503 36.0 32.0 39.0 25.0 40.0 70-71 33.5086326995805 35.5 32.0 38.5 24.5 40.0 72-73 33.09568886153831 35.0 31.5 38.0 24.0 40.0 74-75 32.68505910528259 35.0 31.0 37.0 23.0 39.0 76-77 31.15109536038515 33.5 29.5 35.5 21.5 38.5 78-79 31.970920891612657 34.0 31.0 36.0 22.5 39.0 80-81 31.9307910901711 34.0 31.0 36.0 23.0 37.5 82-83 31.641608118378713 34.0 31.0 35.5 22.0 37.0 84-85 31.335785894061928 34.0 30.5 35.0 20.5 37.0 86-87 31.032363872278474 34.0 30.0 35.0 20.0 36.0 88-89 30.749001844275007 34.0 30.0 35.0 20.0 36.0 90-91 30.462245210379706 34.0 29.0 35.0 19.0 35.5 92-93 30.24714418670264 34.0 29.0 35.0 19.0 35.0 94-95 30.022428078155443 34.0 29.0 35.0 18.0 35.0 96-97 29.811341614290104 34.0 29.0 35.0 18.0 35.0 98-99 29.644359573903305 33.5 29.0 35.0 18.0 35.0 100 27.730762929054244 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30808767694617956 1101 2 0.21552769652870296 1101 3 0.2805463133043915 1101 4 0.1941569397370344 1101 5 0.223546608100051 1101 6 0.1834762814472839 1101 7 0.20974393161765903 1101 8 0.2038604695150923 1101 9 0.23510361524192547 1101 10-11 0.2121007776603392 1101 12-13 0.2303382953538673 1101 14-15 0.3068280681551556 1101 16-17 0.347866880523668 1101 18-19 0.37488697647821567 1101 20-21 0.3582995947488854 1101 22-23 0.32737584819259524 1101 24-25 0.2688833862662534 1101 26-27 0.25772414316389103 1101 28-29 0.3508938574292628 1101 30-31 0.4045528893454673 1101 32-33 0.46947227474479547 1101 34-35 0.38096192075079216 1101 36-37 0.3706701677310491 1101 38-39 0.4178294202810733 1101 40-41 0.40600550069296304 1101 42-43 0.36369434044672744 1101 44-45 0.3606565788996505 1101 46-47 0.4075093258425042 1101 48-49 0.41769562462676646 1101 50-51 0.44495967228009903 1101 52-53 0.3519832683716473 1101 54-55 0.3047937035884303 1101 56-57 0.2976270715716538 1101 58-59 0.39505513924607527 1101 60-61 0.31364395533374534 1101 62-63 0.28612514872269657 1101 64-65 0.34737708998941486 1101 66-67 0.27781730020942774 1101 68-69 0.23812800805072953 1101 70-71 0.17865653278079208 1101 72-73 0.12011313612202912 1101 74-75 0.1689606167915585 1101 76-77 0.22627286955679082 1101 78-79 0.3411906125354385 1101 80-81 0.3031001416221848 1101 82-83 0.2940821383455763 1101 84-85 0.21266159134496476 1101 86-87 0.21883291507822733 1101 88-89 0.2500563408795564 1101 90-91 0.10529512952056663 1101 92-93 0.14580206451520894 1101 94-95 0.19610903573184402 1101 96-97 0.023231780637708255 1101 98-99 -0.030590597295354627 1101 100 0.10641219090663867 1102 1 0.26137324743796597 1102 2 0.1900392988904045 1102 3 0.279067800242629 1102 4 0.17668021568564996 1102 5 0.18518592992273852 1102 6 0.14836746735720396 1102 7 0.12996601526544538 1102 8 0.11642761482915631 1102 9 0.22176472228406396 1102 10-11 0.17820541399025558 1102 12-13 0.2195999956225947 1102 14-15 0.26949817660475617 1102 16-17 0.25478434577713216 1102 18-19 0.24756476197875799 1102 20-21 0.2754668919216101 1102 22-23 0.19556422545388585 1102 24-25 0.2096705961381673 1102 26-27 0.23274535119879403 1102 28-29 0.2589476406682252 1102 30-31 0.2736224410407573 1102 32-33 0.3106953162662265 1102 34-35 0.31142093428002937 1102 36-37 0.3541676987124376 1102 38-39 0.3075807716127201 1102 40-41 0.3564756154803064 1102 42-43 0.2726793674129766 1102 44-45 0.3202922625372082 1102 46-47 0.32552443898001826 1102 48-49 0.36421206133103823 1102 50-51 0.36684780207226453 1102 52-53 0.44356101665169234 1102 54-55 0.42350018634390807 1102 56-57 0.33558645079400407 1102 58-59 0.32461696248534366 1102 60-61 0.3026835140105817 1102 62-63 0.38375827597872814 1102 64-65 0.34723727504172786 1102 66-67 0.39147538570171747 1102 68-69 0.3194297593135005 1102 70-71 0.33117791182749556 1102 72-73 0.4195447126803842 1102 74-75 0.3348567067303989 1102 76-77 0.33296013158831883 1102 78-79 0.3770922408285706 1102 80-81 0.34643797831877876 1102 82-83 0.31987649343732727 1102 84-85 0.3303055220156317 1102 86-87 0.31553780863118064 1102 88-89 0.32550368530758433 1102 90-91 0.30563921364651137 1102 92-93 0.2571986290134731 1102 94-95 0.3027505787899365 1102 96-97 0.309023777711694 1102 98-99 0.25591341716987515 1102 100 0.28956471805753026 1103 1 0.3154813957812266 1103 2 0.28246276187616814 1103 3 0.3326002849373353 1103 4 0.13560377390052025 1103 5 0.1742892522487125 1103 6 0.17364527210638414 1103 7 0.2000580177145963 1103 8 0.2514798301562564 1103 9 0.252221260145582 1103 10-11 0.2536294528119569 1103 12-13 0.2568151565964101 1103 14-15 0.3559898103897794 1103 16-17 0.3460221873818483 1103 18-19 0.3681980514763623 1103 20-21 0.32889124415967785 1103 22-23 0.34957331177206896 1103 24-25 0.3261050910102483 1103 26-27 0.3029600307134146 1103 28-29 0.3392011787712761 1103 30-31 0.3696110696593422 1103 32-33 0.4288386938646127 1103 34-35 0.4330783900260613 1103 36-37 0.48294804566700833 1103 38-39 0.45297888729879077 1103 40-41 0.4287043107385742 1103 42-43 0.5021418538018878 1103 44-45 0.46072584711988895 1103 46-47 0.502788541293107 1103 48-49 0.4565343558624164 1103 50-51 0.4637877968195241 1103 52-53 0.4965715638869739 1103 54-55 0.5628966015802277 1103 56-57 0.5485644864760317 1103 58-59 0.6120668048939635 1103 60-61 0.5721327889255576 1103 62-63 0.655863578729118 1103 64-65 0.6144122591632666 1103 66-67 0.5868173214759835 1103 68-69 0.6657048051933998 1103 70-71 0.6122470733286178 1103 72-73 0.6316296558800616 1103 74-75 0.592619773819159 1103 76-77 0.599757749304711 1103 78-79 0.5643407546578949 1103 80-81 0.5380860867053521 1103 82-83 0.542217539451503 1103 84-85 0.4884469910421636 1103 86-87 0.532614182299664 1103 88-89 0.5855542593789416 1103 90-91 0.6009378946245896 1103 92-93 0.5282285964208526 1103 94-95 0.6343446873238356 1103 96-97 0.6724661953695303 1103 98-99 0.6924960118867425 1103 100 0.7159264062811097 1104 1 0.27448412624883645 1104 2 0.23513979166791188 1104 3 0.2625985368101098 1104 4 0.06273309445415975 1104 5 0.15154116911826065 1104 6 0.16919488352404244 1104 7 0.20648495278249612 1104 8 0.21125579331162925 1104 9 0.26712160552358455 1104 10-11 0.30327420262398874 1104 12-13 0.3107583150258151 1104 14-15 0.39302346921124354 1104 16-17 0.3511799879575719 1104 18-19 0.36136565616281047 1104 20-21 0.2771318784562311 1104 22-23 0.32619125743135413 1104 24-25 0.33864578558527825 1104 26-27 0.3855714393452345 1104 28-29 0.43228324039645827 1104 30-31 0.3712328281139321 1104 32-33 0.35820783451153915 1104 34-35 0.4802420811229595 1104 36-37 0.35268464588307324 1104 38-39 0.4243586317347692 1104 40-41 0.47272358275913007 1104 42-43 0.5451234274552945 1104 44-45 0.5378049612837543 1104 46-47 0.5245488280094435 1104 48-49 0.4890817997637029 1104 50-51 0.558210975724684 1104 52-53 0.5632167867130349 1104 54-55 0.554750103164146 1104 56-57 0.5842834186497967 1104 58-59 0.5606948533054208 1104 60-61 0.6192716757624623 1104 62-63 0.571326882692496 1104 64-65 0.6565649854017721 1104 66-67 0.6638654692864137 1104 68-69 0.6701471793863831 1104 70-71 0.5601454546860509 1104 72-73 0.6554694932418741 1104 74-75 0.6750310061123983 1104 76-77 0.5781884049565846 1104 78-79 0.7204357417875578 1104 80-81 0.775095293386439 1104 82-83 0.6414758159682528 1104 84-85 0.7194079104587807 1104 86-87 0.7236509992673668 1104 88-89 0.7450180959966097 1104 90-91 0.6640647730085583 1104 92-93 0.6244683641363409 1104 94-95 0.6835927156923525 1104 96-97 0.6319774452614197 1104 98-99 0.6952487437298238 1104 100 0.8855222008284045 1105 1 0.24430023326016936 1105 2 0.1415396704529357 1105 3 0.19994456746467648 1105 4 0.1442053720571579 1105 5 0.06952708805312113 1105 6 0.15254618918013563 1105 7 0.17107689110581958 1105 8 0.16084789101006436 1105 9 0.15898547649133832 1105 10-11 0.22821593272863794 1105 12-13 0.2187807367037351 1105 14-15 0.2915073399916679 1105 16-17 0.28227861767141604 1105 18-19 0.31858804175525535 1105 20-21 0.2585831145895412 1105 22-23 0.3177102048846976 1105 24-25 0.34835479054507346 1105 26-27 0.34622094262794434 1105 28-29 0.4090562507420401 1105 30-31 0.44565666282074545 1105 32-33 0.43020149992171497 1105 34-35 0.44013268291923424 1105 36-37 0.4599601088704759 1105 38-39 0.4699687409600273 1105 40-41 0.4693603139919844 1105 42-43 0.44806837206512284 1105 44-45 0.4878502982439983 1105 46-47 0.5370162853288676 1105 48-49 0.5535075430166785 1105 50-51 0.6163025561326734 1105 52-53 0.6006427053775099 1105 54-55 0.565430091768242 1105 56-57 0.5744885506865458 1105 58-59 0.5944411665312543 1105 60-61 0.6431224261585413 1105 62-63 0.6715313223958574 1105 64-65 0.6471094839482561 1105 66-67 0.5817353850743316 1105 68-69 0.6715636223061452 1105 70-71 0.6630980947855392 1105 72-73 0.6173422656645897 1105 74-75 0.671346627510566 1105 76-77 0.6308785893968256 1105 78-79 0.7813891661240469 1105 80-81 0.8350136604303167 1105 82-83 0.7883947499129071 1105 84-85 0.7800820418253274 1105 86-87 0.7352944926611933 1105 88-89 0.7675611524904937 1105 90-91 0.8613439000953527 1105 92-93 0.8032623875103866 1105 94-95 0.8978646183674321 1105 96-97 0.851431137946765 1105 98-99 0.890871052836598 1105 100 1.0271234354578525 1106 1 0.21621799841894784 1106 2 0.20381525484993546 1106 3 0.1986903069133703 1106 4 0.1388822342021001 1106 5 0.14188695037674393 1106 6 0.12797987302219838 1106 7 0.14736291750426744 1106 8 0.1447788842712754 1106 9 0.17878183894268318 1106 10-11 0.18303686975047384 1106 12-13 0.19226057207657732 1106 14-15 0.2678563383238526 1106 16-17 0.30269338697461023 1106 18-19 0.2760931862072553 1106 20-21 0.292136821575113 1106 22-23 0.31354674112937886 1106 24-25 0.30061407657333916 1106 26-27 0.3126995763604299 1106 28-29 0.3176854318071278 1106 30-31 0.33995365098154195 1106 32-33 0.38569818203918516 1106 34-35 0.3751496301320856 1106 36-37 0.37433541461317077 1106 38-39 0.30356682013246683 1106 40-41 0.3586623616934901 1106 42-43 0.36964476944282154 1106 44-45 0.4769271959105055 1106 46-47 0.5455874732167914 1106 48-49 0.48076892442577446 1106 50-51 0.447797586519016 1106 52-53 0.5232541094760776 1106 54-55 0.4256562011092271 1106 56-57 0.468144114605785 1106 58-59 0.5642832800477819 1106 60-61 0.5722266962510503 1106 62-63 0.6381859491740016 1106 64-65 0.5710538127715026 1106 66-67 0.622680565428233 1106 68-69 0.5782765008067798 1106 70-71 0.588105882944653 1106 72-73 0.6630111935443495 1106 74-75 0.612557602559221 1106 76-77 0.52624945628801 1106 78-79 0.6195936596146758 1106 80-81 0.6674508747150689 1106 82-83 0.6413831644212458 1106 84-85 0.647588646706577 1106 86-87 0.5324648710785382 1106 88-89 0.63821563687927 1106 90-91 0.7015786960539714 1106 92-93 0.6902088401490332 1106 94-95 0.7716917452403216 1106 96-97 0.766940001644496 1106 98-99 0.7677700607848443 1106 100 0.8939033665059988 1107 1 0.21768564373420674 1107 2 0.1860464666052266 1107 3 0.2288727792338534 1107 4 0.13961515462445107 1107 5 0.12028045114384867 1107 6 0.1381051367779591 1107 7 0.1342395305274522 1107 8 0.11573919704173363 1107 9 0.1991380343999012 1107 10-11 0.18759967253484433 1107 12-13 0.20286842529164772 1107 14-15 0.20037298400961845 1107 16-17 0.21758355699441267 1107 18-19 0.20778945479717237 1107 20-21 0.26950802961670917 1107 22-23 0.33294897314657845 1107 24-25 0.31465917323549775 1107 26-27 0.3315131253026422 1107 28-29 0.32947894710993353 1107 30-31 0.2506922292982239 1107 32-33 0.2943451476257479 1107 34-35 0.33497556831010655 1107 36-37 0.3844234507530615 1107 38-39 0.3692506731135339 1107 40-41 0.3592620890537148 1107 42-43 0.3450998518304118 1107 44-45 0.4306654657645055 1107 46-47 0.4341689111666298 1107 48-49 0.4543586700655666 1107 50-51 0.3751091073010855 1107 52-53 0.4383320230913199 1107 54-55 0.4648711775708776 1107 56-57 0.4527466829244773 1107 58-59 0.5078747759314908 1107 60-61 0.5019675248628204 1107 62-63 0.5027973506999999 1107 64-65 0.562857740887388 1107 66-67 0.5471966836903874 1107 68-69 0.5179334827966287 1107 70-71 0.5861378024188468 1107 72-73 0.5423443265536889 1107 74-75 0.5679239953109558 1107 76-77 0.49736963589705496 1107 78-79 0.6121213377409553 1107 80-81 0.6110886668359576 1107 82-83 0.6734486896890282 1107 84-85 0.5326297180298205 1107 86-87 0.5442235277114627 1107 88-89 0.4942193112625368 1107 90-91 0.4380306847646871 1107 92-93 0.5773628400893287 1107 94-95 0.6326491632698357 1107 96-97 0.6773455302165843 1107 98-99 0.7517347348773811 1107 100 0.9013691737782885 1108 1 0.22921723442541975 1108 2 0.15206840665291566 1108 3 0.2190542838927385 1108 4 0.15572961802565288 1108 5 0.14060280306234318 1108 6 0.12756681325567598 1108 7 0.16768848796758817 1108 8 0.16298782998918426 1108 9 0.15837224612773326 1108 10-11 0.14543855173005937 1108 12-13 0.18341670362458018 1108 14-15 0.22973153175040295 1108 16-17 0.2716657991669251 1108 18-19 0.27466444530085 1108 20-21 0.27652480879056895 1108 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-0.3370845048379074 2316 80-81 -0.3302177479137569 2316 82-83 -0.2480381983311446 2316 84-85 -0.2669613624123208 2316 86-87 -0.2622679530333869 2316 88-89 -0.18463695081644715 2316 90-91 -0.06402704346911392 2316 92-93 0.015241651948230128 2316 94-95 0.04862404894997496 2316 96-97 0.010151682064311274 2316 98-99 -0.04446303423664588 2316 100 -0.3437408786907348 >>END_MODULE >>Per sequence quality scores pass #Quality Count 8 1.0 9 2.0 10 19.0 11 42.0 12 108.0 13 303.0 14 906.0 15 2334.0 16 5447.0 17 10916.0 18 19521.0 19 30103.0 20 42290.0 21 57611.0 22 73715.0 23 85302.0 24 93586.0 25 104694.0 26 123853.0 27 147787.0 28 180586.0 29 218215.0 30 261797.0 31 317825.0 32 388135.0 33 486030.0 34 635542.0 35 861143.0 36 1254074.0 37 1922865.0 38 1903495.0 39 299039.0 40 35.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.74494602554611 19.77436355405218 13.00943727657595 24.47125314382576 2 15.145161603712104 21.22624204611037 39.689894798863804 23.938701551313716 3 17.357739790873765 26.45816320746993 30.773916110193277 25.410180891463025 4 11.698207554467636 17.303930835120223 38.232795714781886 32.76506589563026 5 12.227777212727096 38.35956935370833 35.88960162247568 13.523051811088898 6 31.064402343506455 38.04875944421388 17.8376956569474 13.049142555332265 7 27.17542528482036 32.74119765671798 22.57749056634074 17.505886492120922 8 25.680225786248084 36.65561729780951 20.151416343934198 17.512740572008205 9 26.311728022513787 14.912717304072354 20.120961638941832 38.65459303447203 10-11 24.39850614879041 26.417951069350977 29.385091569812754 19.798451212045862 12-13 24.944619793958868 24.18584930643147 29.204064815282283 21.665466084327377 14-15 22.19605595318978 25.75107420018702 26.551729494576705 25.501140352046498 16-17 21.917103103630854 28.621266096403847 27.316796582684532 22.144834217280767 18-19 21.69172574827195 27.81634680764101 29.09517237875737 21.39675506532967 20-21 22.435655613468057 27.102127255795516 29.09519622933351 21.367020901402917 22-23 22.162810624456267 27.02293367794229 28.87425168493072 21.94000401267072 24-25 21.927948561812013 27.122673604330537 29.02047412396258 21.92890370989487 26-27 22.00729440124056 27.23208142657731 28.963934929356217 21.796689242825913 28-29 21.942670069897076 27.27396153495943 29.097441568096055 21.685926827047442 30-31 22.20155616137903 27.195934864541492 29.04722613588665 21.555282838192827 32-33 21.940449796967666 27.310509628684798 28.987476086521607 21.761564487825932 34-35 22.19526653460275 27.401175769481373 28.938813329865976 21.4647443660499 36-37 22.210883923537438 27.322097886382863 28.821310902675112 21.64570728740458 38-39 22.078381733359443 27.48381040079902 28.83737441130937 21.600433454532173 40-41 22.21362718595451 27.32758629286079 28.880870120674622 21.577916400510073 42-43 21.99752474247208 27.368296140352278 28.806325137950655 21.82785397922499 44-45 22.08361617455356 27.5384358781678 28.649397003686744 21.7285509435919 46-47 22.021099562329645 27.47248900339525 28.494459854657816 22.011951579617286 48-49 21.993053181699782 27.53570178489636 28.524950796563214 21.946294236840643 50-51 22.035945391422427 27.73242330234139 28.323559957936556 21.90807134829963 52-53 22.10059943621003 27.775746114811955 28.175459475391502 21.948194973586517 54-55 22.01904682532181 27.743554058609337 28.27654418850144 21.960854927567407 56-57 22.083878054527926 27.787740060008115 28.1401630830966 21.988218802367363 58-59 22.030091899507532 27.7253684469348 28.277878121430366 21.9666615321273 60-61 22.17209014075518 27.8261778689003 28.228545468210527 21.773186522133997 62-63 21.99101219512579 27.840307687817965 28.265543035343516 21.903137081712728 64-65 21.92758866651856 27.682232414683966 28.267103237538503 22.123075681258968 66-67 21.925962658278443 27.74619730488066 28.09806800986126 22.229772026979642 68-69 21.86701179374297 27.768066500716504 28.198471093210447 22.166450612330078 70-71 21.864665825985245 27.589990779165984 28.233753941711043 22.31158945313773 72-73 21.871269003349564 27.772885632979623 28.024391677627193 22.33145368604362 74-75 21.863104071674837 27.83182431914347 27.942021358082858 22.363050251098834 76-77 21.754016877413914 27.73493836288497 28.073637049530113 22.437407710170994 78-79 21.747907271440532 27.667768358729354 28.057499886107866 22.526824483722244 80-81 21.73567836310684 27.874185167723596 27.857238115426902 22.532898353742663 82-83 21.83359148365848 27.83021296785077 27.91491993753163 22.421275610959125 84-85 21.789019394055632 27.851764648946453 27.82283232062311 22.5363836363748 86-87 21.648018373198024 27.76596639286254 27.9977730602228 22.58824217371663 88-89 21.632192870239148 27.69768574337058 27.953467001892275 22.716654384498 90-91 21.78450205119432 27.739351366849586 27.87817988137713 22.59796670057896 92-93 21.645376586728858 27.682983706479337 27.87553624185413 22.796103464937673 94-95 21.479599845650252 27.62141786519597 27.939677598023906 22.95930469112988 96-97 21.49715589261162 27.572899755968905 27.993336217256072 22.936608134163404 98-99 21.439288410402995 27.489288604616423 27.88390929162175 23.187513693358834 100 20.5584403545771 28.009056856360033 28.07634919833405 23.356153590728816 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3331.0 1 2459.5 2 2780.0 3 3982.5 4 3012.0 5 2430.0 6 3699.0 7 6761.0 8 10446.5 9 12170.0 10 10516.5 11 8320.5 12 8267.0 13 8954.0 14 9701.0 15 10279.5 16 10880.0 17 11642.5 18 12694.5 19 13881.5 20 15592.5 21 18366.5 22 21947.5 23 27387.0 24 34404.0 25 42979.5 26 53978.5 27 67128.5 28 81473.0 29 98092.5 30 117101.5 31 135726.5 32 156927.0 33 180385.0 34 204179.5 35 225317.5 36 249464.5 37 274381.0 38 291880.5 39 307179.0 40 316421.0 41 322758.0 42 330919.0 43 339127.0 44 346714.0 45 355196.5 46 361219.0 47 362219.5 48 364461.0 49 366175.5 50 362699.0 51 349278.5 52 330273.0 53 314160.5 54 296048.5 55 271727.0 56 243177.0 57 214713.0 58 187974.0 59 160817.5 60 133677.0 61 107865.5 62 85305.5 63 65812.5 64 48253.0 65 34865.5 66 25341.0 67 18667.5 68 14025.5 69 10317.0 70 7787.5 71 5910.5 72 4378.0 73 3236.5 74 2384.0 75 1669.5 76 1094.5 77 698.0 78 455.0 79 298.5 80 211.5 81 154.0 82 106.0 83 74.0 84 52.0 85 40.0 86 30.5 87 17.0 88 14.0 89 13.5 90 9.0 91 7.5 92 6.0 93 3.5 94 3.0 95 3.0 96 2.0 97 3.0 98 5.0 99 5.5 100 4.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0032957848276551193 2 0.06335464082715382 3 6.40263931487141E-4 4 6.087755414140029E-4 5 0.001595411763705663 6 6.192716714383823E-4 7 0.0 8 8.396904019503489E-4 9 3.1488390073138084E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 8.029539468650211E-4 18-19 0.0010811013925110743 20-21 0.003201319657435705 22-23 5.248065012189681E-6 24-25 2.6240325060948403E-5 26-27 1.4694582034131107E-4 28-29 0.0010338688074013672 30-31 0.0016793808039006978 32-33 0.0028024667165092894 34-35 0.0038678239139837947 36-37 0.0012857759279864718 38-39 0.001464210138400921 40-41 0.005295297597299388 42-43 0.0066702906304930846 44-45 0.005038142411702093 46-47 0.0066178099803711875 48-49 0.008412648214540058 50-51 0.007966562688503934 52-53 0.0038730719789959846 54-55 0.0023248928004000287 56-57 0.0028969318867287036 58-59 0.003552940013252414 60-61 0.007441756187284967 62-63 0.013665961291741928 64-65 0.0036264129234230696 66-67 0.009997563848221341 68-69 0.012259479868475094 70-71 0.005515716327811355 72-73 0.0074627484473337264 74-75 0.003101606422204101 76-77 0.0028234589765580484 78-79 0.008008547208601453 80-81 0.006197964779396012 82-83 0.016169288302556404 84-85 0.01726088582509186 86-87 0.022482710512220592 88-89 0.020934531333624634 90-91 0.023726501920109545 92-93 0.03324124378720943 94-95 0.036694470565230244 96-97 0.022047121116208847 98-99 0.018032351381883742 100 0.018053343641932503 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9527321.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.651228629749156 #Duplication Level Percentage of deduplicated Percentage of total 1 71.9470161905282 42.91727912009461 2 17.196755714908864 20.51615213679948 3 5.42622205724241 9.710424375970643 4 2.232475604953802 5.326796508857471 5 1.1011361751992692 3.284206286964956 6 0.5945597244817813 2.127973083546204 7 0.3619937014699627 1.5115358334239732 8 0.23529124368694576 1.122832941739842 9 0.155931003653901 0.837132835448259 >10 0.6956196314647366 6.962580879335365 >50 0.03224384211320704 1.3126341088653841 >100 0.018729213636704968 2.0533706559111247 >500 0.0014082843941592125 0.5757536620611211 >1k 5.187943048277449E-4 0.5870002840481846 >5k 2.470449029084938E-5 0.08044672810725452 >10k+ 7.411347087254813E-5 1.0738805588261313 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 28445 0.29856241854347093 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26464 0.2777695849651754 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 17963 0.18854198362792648 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 2.0992260048758723E-5 2 0.0 0.0 0.0 1.0496130024379361E-5 2.0992260048758723E-5 3 0.0 0.0 0.0 1.0496130024379361E-5 2.0992260048758723E-5 4 0.0 0.0 0.0 2.0992260048758723E-5 3.1488390073138084E-5 5 0.0 0.0 0.0 3.1488390073138084E-5 3.1488390073138084E-5 6 4.1984520097517445E-5 0.0 0.0 3.1488390073138084E-5 7.347291017065552E-5 7 4.1984520097517445E-5 0.0 0.0 3.1488390073138084E-5 7.347291017065552E-5 8 4.1984520097517445E-5 0.0 0.0 3.1488390073138084E-5 8.396904019503489E-5 9 4.1984520097517445E-5 0.0 0.0 3.1488390073138084E-5 1.1545743026817297E-4 10-11 4.1984520097517445E-5 0.0 0.0 3.1488390073138084E-5 1.1545743026817297E-4 12-13 4.1984520097517445E-5 0.0 0.0 3.1488390073138084E-5 1.1545743026817297E-4 14-15 4.1984520097517445E-5 1.5744195036569042E-5 0.0 3.1488390073138084E-5 1.626900153778801E-4 16-17 4.1984520097517445E-5 2.0992260048758723E-5 0.0 3.673645508532776E-5 1.9942647046320786E-4 18-19 4.1984520097517445E-5 2.0992260048758723E-5 0.0 4.1984520097517445E-5 2.0992260048758723E-4 20-21 4.1984520097517445E-5 2.0992260048758723E-5 0.0 6.297678014627617E-5 2.3091486053634594E-4 22-23 4.1984520097517445E-5 2.0992260048758723E-5 0.0 8.921710520722457E-5 2.7289938063386335E-4 24-25 4.1984520097517445E-5 2.0992260048758723E-5 0.0 2.5190712058510467E-4 3.148839007313808E-4 26-27 4.1984520097517445E-5 2.0992260048758723E-5 0.0 5.038142411702093E-4 3.3587616078013956E-4 28-29 4.1984520097517445E-5 2.0992260048758723E-5 0.0 0.0015901636986934732 3.3587616078013956E-4 30-31 4.1984520097517445E-5 2.0992260048758723E-5 0.0 0.00574138312333551 3.411242257923292E-4 32-33 4.1984520097517445E-5 2.0992260048758723E-5 0.0 0.014841527854472417 3.6736455085327767E-4 34-35 4.1984520097517445E-5 3.673645508532776E-5 0.0 0.02906378403750645 3.726126158654673E-4 36-37 4.1984520097517445E-5 5.248065012189681E-5 0.0 0.05350927086428599 3.8835681090203635E-4 38-39 4.1984520097517445E-5 7.347291017065552E-5 0.0 0.0903769275749185 4.0410100593860543E-4 40-41 4.1984520097517445E-5 8.396904019503489E-5 0.0 0.13170019148090004 4.1459713596298474E-4 42-43 4.1984520097517445E-5 8.396904019503489E-5 0.0 0.17183739269412673 4.2509326598736416E-4 44-45 4.723258510970713E-5 8.396904019503489E-5 0.0 0.223362894983805 4.355893960117435E-4 46-47 5.248065012189681E-5 8.396904019503489E-5 0.0 0.28753623395286043 4.5133359104831256E-4 48-49 5.248065012189681E-5 8.396904019503489E-5 0.0 0.3564695678879719 4.5133359104831256E-4 50-51 6.297678014627617E-5 8.396904019503489E-5 0.0 0.4315431378873452 4.5133359104831256E-4 52-53 6.297678014627617E-5 8.396904019503489E-5 0.0 0.5055093661691467 4.933181111458299E-4 54-55 6.297678014627617E-5 9.446517021941426E-5 0.0 0.5905332674316317 5.353026312433474E-4 56-57 6.297678014627617E-5 1.0496130024379361E-4 0.0 0.6918314182969167 5.353026312433474E-4 58-59 6.297678014627617E-5 1.0496130024379361E-4 0.0 0.8056409561512623 5.40550696255537E-4 60-61 6.297678014627617E-5 1.0496130024379361E-4 0.0 0.9295950036741703 5.457987612677268E-4 62-63 6.297678014627617E-5 1.0496130024379361E-4 0.0 1.0563095333934902 5.772871513408649E-4 64-65 6.297678014627617E-5 1.0496130024379361E-4 0.0 1.1912582771169355 5.877832813652442E-4 66-67 6.297678014627617E-5 1.1545743026817297E-4 0.0 1.347949754185883 5.982794113896236E-4 68-69 6.297678014627617E-5 1.1545743026817297E-4 0.0 1.5299631449386455 5.982794113896236E-4 70-71 6.297678014627617E-5 1.1545743026817297E-4 0.0 1.7319506711278017 6.297678014627617E-4 72-73 8.396904019503489E-5 1.1545743026817297E-4 0.0 1.9338069956916535 7.347291017065553E-4 74-75 8.396904019503489E-5 1.1545743026817297E-4 0.0 2.1404915400667197 7.347291017065553E-4 76-77 8.396904019503489E-5 1.1545743026817297E-4 0.0 2.372146377769784 7.347291017065553E-4 78-79 8.396904019503489E-5 1.1545743026817297E-4 0.0 2.639330615605373 7.347291017065553E-4 80-81 8.396904019503489E-5 1.2595356029255234E-4 0.0 2.925245197469467 7.71465556791883E-4 82-83 8.396904019503489E-5 1.364496903169317E-4 0.0 3.221262304482026 8.344423369381591E-4 84-85 8.396904019503489E-5 1.364496903169317E-4 0.0 3.5195728159049118 8.396904019503489E-4 86-87 9.446517021941425E-5 1.364496903169317E-4 0.0 3.836178081960291 8.449384669625386E-4 88 9.446517021941425E-5 1.364496903169317E-4 0.0 4.093826585668731 8.606826619991076E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 15000 0.0 46.147488 1 GTATCAA 25065 0.0 34.821045 1 TACCTGG 9080 0.0 33.714916 2 GTACCTG 10015 0.0 31.647577 1 TCAACGC 29895 0.0 28.655754 4 ATCAACG 30005 0.0 28.441368 3 CAACGCA 30285 0.0 28.317766 5 GTACATG 30250 0.0 27.422352 1 AACGCAG 31660 0.0 27.161846 6 TATCAAC 31970 0.0 26.843996 2 ACCTGGG 11125 0.0 26.780428 3 TACATGG 30790 0.0 26.452404 2 ACATGGG 30945 0.0 25.132505 3 ACGCAGA 36455 0.0 23.40861 7 CGCAGAG 37075 0.0 22.890404 8 CATGGGG 17490 0.0 22.461573 4 GCAGAGT 42000 0.0 19.791845 9 GAGTACT 25575 0.0 19.421309 12-13 ATGGGGG 10350 0.0 18.705984 5 AGAGTAC 38025 0.0 18.653986 10-11 >>END_MODULE