FastQCFastQC Report
Mon 6 Jun 2016
ERR1211516_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211516_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12556492
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT396550.3158127285869334No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT338620.26967723150701645No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT220210.17537541536282586No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA193850.048.5085681
GTATCAA341500.038.194011
TCAACGC406400.031.2190114
CAACGCA411000.030.8560755
ATCAACG411550.030.8181553
AACGCAG427600.029.7950046
TACCTGG108050.028.9408022
TATCAAC442850.028.8394362
GTACATG432550.028.6411691
GTACCTG118400.027.6400811
TACATGG442200.027.2654692
ACATGGG443850.026.3097383
ACGCAGA484700.026.1120057
CGCAGAG498000.025.2542638
CATGGGG253200.023.0497884
ACCTGGG135600.022.4911353
GCAGAGT554150.021.8163329
GAGTACT331300.020.69223612-13
ATGGGGG155050.020.0616155
AGAGTAC503150.019.53535810-11
Produced by FastQC (version 0.11.5)