FastQCFastQC Report
Mon 6 Jun 2016
ERR1211516_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211516_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12556492
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT424670.33820751846933045No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT366500.29188088520265054No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT261220.20803581127595192No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA205950.046.2163731
GTATCAA353950.037.4541851
TACCTGG103800.030.1268792
TCAACGC429200.030.0989114
CAACGCA435800.029.664055
ATCAACG438250.029.4453073
AACGCAG452950.028.5815876
TATCAAC462150.028.144862
GTACATG448450.027.2021431
GTACCTG121650.026.4418071
TACATGG458250.026.0241552
ACATGGG456400.025.2162593
ACGCAGA522600.024.574437
CGCAGAG537000.023.8191958
CATGGGG249550.022.9175824
ACCTGGG135800.022.3893763
GCAGAGT599000.020.9378249
GAGTACT354850.019.91704612-13
AGAGTAC545400.019.5322110-11
ATGGGGG143100.019.3748995
Produced by FastQC (version 0.11.5)