##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211516_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 12556492 Sequences flagged as poor quality 0 Sequence length 100 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.08994717632918 33.0 31.0 34.0 30.0 34.0 2 32.31382690324654 34.0 31.0 34.0 30.0 34.0 3 32.32982540027899 34.0 31.0 34.0 30.0 34.0 4 35.977182082384154 37.0 35.0 37.0 35.0 37.0 5 35.89218835961509 37.0 35.0 37.0 35.0 37.0 6 35.909458071569674 37.0 35.0 37.0 35.0 37.0 7 35.828121421174004 37.0 35.0 37.0 35.0 37.0 8 35.820032935950586 37.0 35.0 37.0 35.0 37.0 9 37.4676985419176 39.0 37.0 39.0 34.0 39.0 10-11 37.42861298362632 39.0 37.0 39.0 34.0 39.0 12-13 37.33933796955392 39.0 37.0 39.0 34.0 39.0 14-15 38.56464193980293 40.0 38.0 41.0 34.0 41.0 16-17 38.49059124156651 40.0 38.0 41.0 34.0 41.0 18-19 38.46914373058972 40.0 38.0 41.0 34.0 41.0 20-21 38.40557800697839 40.0 38.0 41.0 34.0 41.0 22-23 38.29876541154966 40.0 38.0 41.0 34.0 41.0 24-25 38.18570449453557 40.0 38.0 41.0 33.0 41.0 26-27 38.00368474730044 40.0 37.0 41.0 33.0 41.0 28-29 38.04851470458469 40.0 37.5 41.0 33.0 41.0 30-31 37.9805569501418 40.0 37.0 41.0 33.0 41.0 32-33 37.80685887427794 40.0 37.0 41.0 33.0 41.0 34-35 37.69927803083855 40.0 37.0 41.0 33.0 41.0 36-37 37.64486681471226 40.0 37.0 41.0 32.5 41.0 38-39 37.549357137327846 40.0 37.0 41.0 32.0 41.0 40-41 37.38704161958611 40.0 36.0 41.0 31.0 41.0 42-43 37.233763339314834 40.0 36.0 41.0 31.0 41.0 44-45 37.0485012055915 39.0 36.0 41.0 31.0 41.0 46-47 36.79320601645746 39.0 35.5 41.0 30.0 41.0 48-49 36.57194760288144 39.0 35.0 41.0 30.0 41.0 50-51 36.369129690044005 39.0 35.0 40.0 29.0 41.0 52-53 36.16235920032442 39.0 35.0 40.0 28.5 41.0 54-55 35.957685952414096 38.5 35.0 40.0 28.0 41.0 56-57 35.75153422627912 38.0 34.0 40.0 28.0 41.0 58-59 35.48146990417388 38.0 34.0 40.0 27.0 41.0 60-61 35.15821082831097 38.0 34.0 40.0 26.5 41.0 62-63 34.82517250837256 37.0 33.5 40.0 26.0 41.0 64-65 34.49834213250006 37.0 33.0 39.5 26.0 41.0 66-67 34.14798978090377 36.0 33.0 39.0 26.0 40.5 68-69 33.73747834984485 36.0 32.0 39.0 25.0 40.0 70-71 33.347881398721874 35.0 32.0 38.5 24.5 40.0 72-73 32.93621176201124 35.0 31.0 37.5 23.5 40.0 74-75 32.52424510763038 35.0 31.0 37.0 22.5 39.0 76-77 30.985384612199013 33.5 29.5 35.5 21.0 38.0 78-79 31.82995879740934 34.0 30.5 36.0 22.0 38.0 80-81 31.805665069511452 34.0 31.0 36.0 22.5 37.5 82-83 31.528057159595214 34.0 31.0 35.0 21.5 37.0 84-85 31.230660641523123 34.0 30.0 35.0 20.5 36.5 86-87 30.938437503086053 34.0 30.0 35.0 20.0 36.0 88-89 30.656315991759485 34.0 29.5 35.0 20.0 36.0 90-91 30.37061346433383 34.0 29.0 35.0 19.0 35.5 92-93 30.15948144593251 34.0 29.0 35.0 18.5 35.0 94-95 29.9373408592145 34.0 29.0 35.0 18.0 35.0 96-97 29.724646382126473 34.0 29.0 35.0 18.0 35.0 98-99 29.555465332196285 33.0 29.0 35.0 17.5 35.0 100 27.649230772416374 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.30716256694599764 1101 2 0.2369030331962847 1101 3 0.29990527124307675 1101 4 0.16937586822898965 1101 5 0.17697217335381765 1101 6 0.22070192869294658 1101 7 0.22947441531528767 1101 8 0.2262099627774674 1101 9 0.2537745731002303 1101 10-11 0.2234605651343955 1101 12-13 0.24155079467497842 1101 14-15 0.31397427255012644 1101 16-17 0.32165062334296834 1101 18-19 0.3634772929082217 1101 20-21 0.3244213359194603 1101 22-23 0.3095215088965162 1101 24-25 0.29349817521749344 1101 26-27 0.30013856001601624 1101 28-29 0.36829711055069936 1101 30-31 0.36290096858399323 1101 32-33 0.41754766289184175 1101 34-35 0.4429257973308225 1101 36-37 0.47502950467122673 1101 38-39 0.4595441159657625 1101 40-41 0.42222468827237947 1101 42-43 0.4881206741398074 1101 44-45 0.4396108158789289 1101 46-47 0.5752535563565218 1101 48-49 0.5574174496879749 1101 50-51 0.4902431808960017 1101 52-53 0.46339098951182933 1101 54-55 0.419335530917607 1101 56-57 0.39335474851293384 1101 58-59 0.4312474150384773 1101 60-61 0.3944355539894886 1101 62-63 0.3809740195221494 1101 64-65 0.3590657763345675 1101 66-67 0.34826180563958076 1101 68-69 0.3550119841334407 1101 70-71 0.3113767738051578 1101 72-73 0.17942718749716136 1101 74-75 0.2527947024192514 1101 76-77 0.29657208981774374 1101 78-79 0.3750746832361038 1101 80-81 0.3069676963786385 1101 82-83 0.29458944077591553 1101 84-85 0.22560256080244656 1101 86-87 0.2527713151668003 1101 88-89 0.23359599097856076 1101 90-91 0.20759396632370297 1101 92-93 0.07561899384840132 1101 94-95 0.09359886550639729 1101 96-97 0.019962281302344564 1101 98-99 -0.053888167104208406 1101 100 -0.0024399622267452514 1102 1 0.31581575724964495 1102 2 0.2724695982925667 1102 3 0.31949948440300346 1102 4 0.23732868359808634 1102 5 0.2277672379421034 1102 6 0.18911237679683524 1102 7 0.21322265347404823 1102 8 0.2066424292668998 1102 9 0.25074132233589097 1102 10-11 0.2190330960610538 1102 12-13 0.3170085743608837 1102 14-15 0.34581900859293313 1102 16-17 0.3624462713721215 1102 18-19 0.3577235880651841 1102 20-21 0.3647090849473855 1102 22-23 0.3592281700790565 1102 24-25 0.28555997590181903 1102 26-27 0.259660554850889 1102 28-29 0.392722278202676 1102 30-31 0.38558026197525663 1102 32-33 0.42472748662904536 1102 34-35 0.39797059072692065 1102 36-37 0.46519273027317354 1102 38-39 0.46148321618805 1102 40-41 0.4226541115260787 1102 42-43 0.3201845637118197 1102 44-45 0.38713233574724626 1102 46-47 0.39747141810197917 1102 48-49 0.44595340662454674 1102 50-51 0.40795867855090506 1102 52-53 0.4456515869527067 1102 54-55 0.4242303789859321 1102 56-57 0.44719932987845823 1102 58-59 0.42873446620644273 1102 60-61 0.45914936136001216 1102 62-63 0.4952350108176873 1102 64-65 0.48103347465627166 1102 66-67 0.4371556636295608 1102 68-69 0.4559860582855606 1102 70-71 0.39517733939626254 1102 72-73 0.46269110813811665 1102 74-75 0.4131813756032727 1102 76-77 0.5276701165324589 1102 78-79 0.48804263816397864 1102 80-81 0.4354683896670615 1102 82-83 0.3871783249620826 1102 84-85 0.36644502270931767 1102 86-87 0.3384075864298879 1102 88-89 0.32650333941491283 1102 90-91 0.3035696746492711 1102 92-93 0.17573234513912084 1102 94-95 0.25070162653695505 1102 96-97 0.345401368035926 1102 98-99 0.2672095087245978 1102 100 0.2741548007228758 1103 1 0.32571965519235135 1103 2 0.3002647984324085 1103 3 0.3439952171119742 1103 4 0.17975007698349543 1103 5 0.2144723664390895 1103 6 0.206750046107679 1103 7 0.21007341740953933 1103 8 0.23798571659392564 1103 9 0.27307419674688305 1103 10-11 0.2553927716502855 1103 12-13 0.2669677292875505 1103 14-15 0.37435378762152993 1103 16-17 0.38469543108366366 1103 18-19 0.35593430699444895 1103 20-21 0.3676775588204819 1103 22-23 0.39889665621223713 1103 24-25 0.37097154112463215 1103 26-27 0.37428907333546846 1103 28-29 0.41736492050799257 1103 30-31 0.5024148293867867 1103 32-33 0.4929434954008727 1103 34-35 0.492599648719775 1103 36-37 0.5009256833481572 1103 38-39 0.462370011962264 1103 40-41 0.5193445462190951 1103 42-43 0.50696287171656 1103 44-45 0.5052283097690946 1103 46-47 0.5521384445356432 1103 48-49 0.5057326376242202 1103 50-51 0.4404971656500365 1103 52-53 0.469806190642025 1103 54-55 0.5847410261578503 1103 56-57 0.617644845165394 1103 58-59 0.6277835704291945 1103 60-61 0.6285899243957616 1103 62-63 0.6349995357218319 1103 64-65 0.7076728501887004 1103 66-67 0.6315842517859807 1103 68-69 0.6415043259737558 1103 70-71 0.6420650338972038 1103 72-73 0.5617380643844996 1103 74-75 0.537189412031573 1103 76-77 0.5787204825605308 1103 78-79 0.5860515412796552 1103 80-81 0.5960291808366982 1103 82-83 0.5248739229961394 1103 84-85 0.4287260544486493 1103 86-87 0.5484093866870232 1103 88-89 0.5955385063236456 1103 90-91 0.6010036413405295 1103 92-93 0.47774089452184754 1103 94-95 0.6220207637832758 1103 96-97 0.6417682313463011 1103 98-99 0.5336993261839886 1103 100 0.6036737821141891 1104 1 0.26460073067462986 1104 2 0.21651567327617016 1104 3 0.24985172040299375 1104 4 0.08945307613226561 1104 5 0.12709453008465488 1104 6 0.14389919241644833 1104 7 0.17082983728172252 1104 8 0.17538481249481208 1104 9 0.22316271189527725 1104 10-11 0.25284772893271423 1104 12-13 0.26933543580832975 1104 14-15 0.3414589453058241 1104 16-17 0.30293634707471995 1104 18-19 0.29658534723824914 1104 20-21 0.2210548191944639 1104 22-23 0.29739840941306994 1104 24-25 0.3085046323386038 1104 26-27 0.32960397610186476 1104 28-29 0.410314106819996 1104 30-31 0.33761482924140296 1104 32-33 0.3395671244589593 1104 34-35 0.43655950244181696 1104 36-37 0.3095811536299635 1104 38-39 0.39333700472440825 1104 40-41 0.4675033572319691 1104 42-43 0.5271903640071756 1104 44-45 0.45933726986127965 1104 46-47 0.42834338268669825 1104 48-49 0.4230705832029358 1104 50-51 0.4663976999675228 1104 52-53 0.4124305131542556 1104 54-55 0.399970797655989 1104 56-57 0.49811057178413876 1104 58-59 0.5439171835994614 1104 60-61 0.43202143507784996 1104 62-63 0.42188724654902643 1104 64-65 0.5156560117960183 1104 66-67 0.4990873742881732 1104 68-69 0.5065458922335466 1104 70-71 0.4217430195106502 1104 72-73 0.5285833382978709 1104 74-75 0.5538175918319723 1104 76-77 0.5153061721417522 1104 78-79 0.5330377406054048 1104 80-81 0.593466157659325 1104 82-83 0.4612650631947375 1104 84-85 0.5257822846870503 1104 86-87 0.6334469465651829 1104 88-89 0.622120218396983 1104 90-91 0.47315569198319807 1104 92-93 0.4892737795006532 1104 94-95 0.5333747800364819 1104 96-97 0.48116918744235804 1104 98-99 0.48872940877538085 1104 100 0.7048127276366465 1105 1 0.21886276517906822 1105 2 0.13765371909165935 1105 3 0.2239211019964742 1105 4 0.13851989790329355 1105 5 0.09411138080833581 1105 6 0.13558946000770788 1105 7 0.13061475214704643 1105 8 0.13896449202884753 1105 9 0.18054287933303215 1105 10-11 0.19584039743555337 1105 12-13 0.2039429930254144 1105 14-15 0.2329985690279628 1105 16-17 0.27528983231051996 1105 18-19 0.28145913893216346 1105 20-21 0.1775495753632228 1105 22-23 0.2793247454359218 1105 24-25 0.30726882822357027 1105 26-27 0.2768817433941777 1105 28-29 0.3299135725133553 1105 30-31 0.37950391920045234 1105 32-33 0.4049663951606206 1105 34-35 0.3991569296985986 1105 36-37 0.4082715080843542 1105 38-39 0.46055255616607127 1105 40-41 0.45801363208494195 1105 42-43 0.44118726095364025 1105 44-45 0.4848164950255409 1105 46-47 0.512971822257235 1105 48-49 0.5378064664679911 1105 50-51 0.5696843049368567 1105 52-53 0.5951498169488048 1105 54-55 0.501853918492138 1105 56-57 0.5633172346111621 1105 58-59 0.6063878931739666 1105 60-61 0.6773294358145847 1105 62-63 0.6261281372823504 1105 64-65 0.5951875601453978 1105 66-67 0.5718488703757671 1105 68-69 0.6558355453845905 1105 70-71 0.6583525649514073 1105 72-73 0.6102527525245662 1105 74-75 0.6790476316099046 1105 76-77 0.5916138816436778 1105 78-79 0.7149452390268074 1105 80-81 0.7326450168285028 1105 82-83 0.7423191675400354 1105 84-85 0.7382259828286486 1105 86-87 0.688157661780096 1105 88-89 0.7110046306881479 1105 90-91 0.7493749905493949 1105 92-93 0.7088640101621415 1105 94-95 0.7502571456085327 1105 96-97 0.7931380061647921 1105 98-99 0.7909095337237027 1105 100 1.0410314044946993 1106 1 0.22440120186300305 1106 2 0.17683705894093293 1106 3 0.2134872471259115 1106 4 0.13011008504646782 1106 5 0.13696816376706522 1106 6 0.12057148574790943 1106 7 0.1521300124395779 1106 8 0.13669089378984722 1106 9 0.13545584589966353 1106 10-11 0.1468473731998614 1106 12-13 0.17247042334940943 1106 14-15 0.21801022640414658 1106 16-17 0.2492936235070573 1106 18-19 0.23268731892456174 1106 20-21 0.262728812104001 1106 22-23 0.2607861249371979 1106 24-25 0.2651111812553921 1106 26-27 0.22890232638427932 1106 28-29 0.3023758521605515 1106 30-31 0.3163333101576953 1106 32-33 0.34624834264291593 1106 34-35 0.34823194412452807 1106 36-37 0.37398062725698367 1106 38-39 0.31628402503012865 1106 40-41 0.3522206405866726 1106 42-43 0.35906884024652186 1106 44-45 0.41896329652170294 1106 46-47 0.4578151778060757 1106 48-49 0.4637772691094355 1106 50-51 0.4548974387762783 1106 52-53 0.48223138339198357 1106 54-55 0.41191398553721825 1106 56-57 0.46155197288570804 1106 58-59 0.49258033725341477 1106 60-61 0.5340862285972108 1106 62-63 0.4972849054319255 1106 64-65 0.5919657362255677 1106 66-67 0.5325748361264928 1106 68-69 0.6078425855307259 1106 70-71 0.5252642113573458 1106 72-73 0.5335397793731218 1106 74-75 0.5708752717280206 1106 76-77 0.5467750981376369 1106 78-79 0.6378412429147708 1106 80-81 0.6698429037716203 1106 82-83 0.6382387059849854 1106 84-85 0.5804202461279253 1106 86-87 0.5264404824825739 1106 88-89 0.5859676444614585 1106 90-91 0.6183890035273301 1106 92-93 0.6299796099321782 1106 94-95 0.7442651702767655 1106 96-97 0.7692704765061613 1106 98-99 0.8014311238295377 1106 100 0.769199226990672 1107 1 0.205205475637662 1107 2 0.17516956819331853 1107 3 0.20579375756810236 1107 4 0.13431594600131547 1107 5 0.09992336334751428 1107 6 0.14097934945755242 1107 7 0.16804759763658694 1107 8 0.16210363486244717 1107 9 0.1329866753559088 1107 10-11 0.16801695243378134 1107 12-13 0.185887961533588 1107 14-15 0.2568297364263046 1107 16-17 0.19795216239229774 1107 18-19 0.1653534179577676 1107 20-21 0.1969091413907691 1107 22-23 0.24044450964186126 1107 24-25 0.29407492073396213 1107 26-27 0.33432286445808046 1107 28-29 0.3224220627499079 1107 30-31 0.2199633116473123 1107 32-33 0.24762045020688106 1107 34-35 0.29296615831112405 1107 36-37 0.39477179823866493 1107 38-39 0.37646888675764956 1107 40-41 0.38642866641863094 1107 42-43 0.36263732554358796 1107 44-45 0.34992320766247076 1107 46-47 0.3500572315677175 1107 48-49 0.38212730775949666 1107 50-51 0.3061458346566823 1107 52-53 0.31617276641836867 1107 54-55 0.35404668031744535 1107 56-57 0.40115338637532716 1107 58-59 0.4766217869963185 1107 60-61 0.49078114000067785 1107 62-63 0.48447473809071795 1107 64-65 0.5855836666487804 1107 66-67 0.4846395173925302 1107 68-69 0.4471127219750173 1107 70-71 0.5791438409075198 1107 72-73 0.5306699814642784 1107 74-75 0.5330638244808981 1107 76-77 0.41108857180605085 1107 78-79 0.5117451544928677 1107 80-81 0.5219568926899321 1107 82-83 0.5359273748104663 1107 84-85 0.42664949534225016 1107 86-87 0.4005660713435404 1107 88-89 0.4324645345792959 1107 90-91 0.34607953322705853 1107 92-93 0.46054798420093235 1107 94-95 0.6325257198962113 1107 96-97 0.6618164120805297 1107 98-99 0.7574437101942664 1107 100 0.8740416511354283 1108 1 0.2403633340939777 1108 2 0.16005646323553435 1108 3 0.21817475591618773 1108 4 0.10105942550065095 1108 5 0.09832872294083472 1108 6 0.11023243703836982 1108 7 0.1279032854691522 1108 8 0.11111957428907715 1108 9 0.13025156850213904 1108 10-11 0.1308164242392067 1108 12-13 0.1627989959631293 1108 14-15 0.1702419261393331 1108 16-17 0.2539853027681147 1108 18-19 0.24602339894033776 1108 20-21 0.23189267338732833 1108 22-23 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-0.34915256333777833 2316 78-79 -0.25645080995619196 2316 80-81 -0.2249880266027624 2316 82-83 -0.13592169122483 2316 84-85 -0.18058886870961643 2316 86-87 -0.17122816720548784 2316 88-89 -0.1432551635413013 2316 90-91 -0.005304540986411865 2316 92-93 0.09988364463412935 2316 94-95 0.15940304079031264 2316 96-97 0.18892387258741294 2316 98-99 0.0040752110109743 2316 100 -0.063066071207448 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 2.0 8 1.0 9 8.0 10 26.0 11 58.0 12 150.0 13 402.0 14 1253.0 15 3123.0 16 7036.0 17 14399.0 18 25368.0 19 39609.0 20 56323.0 21 77519.0 22 101275.0 23 116575.0 24 128264.0 25 143370.0 26 168385.0 27 202059.0 28 244527.0 29 293775.0 30 352954.0 31 425559.0 32 519346.0 33 650211.0 34 846084.0 35 1151716.0 36 1676565.0 37 2553787.0 38 2406025.0 39 350700.0 40 38.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.3149116251154 19.916747743081107 13.41011859137933 24.358222040424156 2 15.05892628526323 21.1223189108011 39.56006807293894 24.258686730996736 3 17.358960205402774 26.57037210417665 30.56000990092465 25.510657789495927 4 11.966164835091321 17.165455969718717 37.82375113129524 33.04462806389473 5 12.543628570215745 38.40733389246926 35.477604471834375 13.571433065480626 6 31.380192488170184 37.82784057351434 17.501597590648537 13.290369347666944 7 27.27691778882191 32.69579592771611 22.432969335703 17.594316947758976 8 25.98796435977457 36.2538341084775 20.013699814930646 17.74450171681729 9 26.517412630234453 14.909900371035247 20.045930036652763 38.526756962077535 10-11 24.659944831725294 26.357162494110614 28.89097129994588 20.091921374218213 12-13 25.151606834138068 24.044331012196718 28.946189747900924 21.857872405764287 14-15 22.523802826458216 25.490172732957582 26.19924816580937 25.786776274774837 16-17 22.15871680186257 28.465173779588326 26.942086982913942 22.43402243563516 18-19 22.043955739395134 27.58324905995831 28.82571920934398 21.547075991302574 20-21 22.71578411416244 26.797367193824424 28.886935789062353 21.599912902950784 22-23 22.509923353586604 26.65921952239682 28.654171774582565 22.17668534943401 24-25 22.300336320102193 26.88132989394329 28.735227758712824 22.08310602724169 26-27 22.267549897984857 26.989000649784582 28.722513470944737 22.02093598128582 28-29 22.24841212044757 26.869151174925744 28.923702299900732 21.958734404725952 30-31 22.46978937229247 26.88419038042373 28.77062564959913 21.87539459768468 32-33 22.242817711403916 27.035462111288517 28.69571658026477 22.026003597042802 34-35 22.484651484067182 27.05639928772007 28.74546577006079 21.713483458151963 36-37 22.52844924511318 26.945512241711917 28.612202468830024 21.913836044344883 38-39 22.408346199357997 27.159369707349107 28.606843275652448 21.825440817640445 40-41 22.412601876603432 26.975103130996196 28.716710008923314 21.89558498347706 42-43 22.2896033832641 27.009604533340692 28.578371925610913 22.122420157784294 44-45 22.309886126125956 27.20475275824315 28.43658710810982 22.04877400752108 46-47 22.340853115808834 27.174771435565404 28.22878636559183 22.25558908303393 48-49 22.320514625837447 27.277835158782015 28.215734487296835 22.185915728083707 50-51 22.362011133227618 27.408075287612494 28.033698596194483 22.196214982965405 52-53 22.416418166969816 27.4147106192425 27.937252877464815 22.23161833632287 54-55 22.32261594443555 27.4352014896191 28.000012742678337 22.242169823267016 56-57 22.35873462791922 27.510952886926574 27.82125465145029 22.309057833703918 58-59 22.334587225464205 27.384485665298726 28.036770004453608 22.244157104783458 60-61 22.461733630241852 27.53576947733391 27.924862573350694 22.077634319073542 62-63 22.278760587037695 27.501715778234114 28.01819559270505 22.201328042023135 64-65 22.235068198515158 27.31215527266444 28.029129593007852 22.423646935812545 66-67 22.240672661685565 27.422171741373823 27.850804959659726 22.486350637280886 68-69 22.143985353363604 27.457373535385145 27.934830082554196 22.46381102869705 70-71 22.199893937661674 27.283666419809105 27.946024408514504 22.570415234014717 72-73 22.17390513084661 27.483783429360216 27.714417596261935 22.627893843531236 74-75 22.142491385386162 27.57218393925296 27.656138917594426 22.629185757766454 76-77 22.03933977229037 27.48494210692831 27.779165104513755 22.69655301626756 78-79 22.053326318116813 27.379196187443274 27.761102567754026 22.80637492668589 80-81 22.02698968560059 27.631568415432668 27.519134289887543 22.8223076090792 82-83 22.113238584716026 27.550092859252516 27.58923823551808 22.747430320513374 84-85 22.01712469538885 27.55166735162315 27.52636133259409 22.904846620393904 86-87 21.892474663468036 27.54775858708234 27.68074316829502 22.87902358115461 88-89 21.886415045673925 27.413968636471857 27.645132210509242 23.054484107344972 90-91 22.02255619662117 27.44001757917449 27.60466103033256 22.932765193871777 92-93 21.94357555324902 27.391401524341894 27.562036454619065 23.102986467790025 94-95 21.774722839974654 27.24568256248081 27.63255616297744 23.347038434567096 96-97 21.782983947619385 27.199670661573222 27.661492448571938 23.355852942235455 98-99 21.68187061062611 27.161993343530938 27.597427437640953 23.558708608201993 100 20.863208209199296 27.629874562560445 27.736650675871687 23.770266552368565 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 4238.0 1 3096.5 2 3030.5 3 4276.5 4 3514.5 5 3031.0 6 4985.5 7 9776.5 8 15190.0 9 17587.5 10 15078.5 11 11842.0 12 11792.5 13 12627.0 14 13475.5 15 14219.5 16 14918.0 17 15916.5 18 17126.0 19 18405.5 20 20282.0 21 23218.5 22 27428.0 23 33895.5 24 42213.0 25 52568.5 26 65853.0 27 81870.5 28 99314.5 29 118476.5 30 140868.0 31 164088.0 32 191366.0 33 222899.5 34 253963.5 35 281987.0 36 316984.5 37 350153.0 38 370844.0 39 390961.5 40 400490.0 41 403770.5 42 411143.5 43 422015.5 44 435821.5 45 451645.0 46 461132.5 47 463437.5 48 471873.5 49 478738.5 50 478835.5 51 467835.5 52 448948.5 53 434957.0 54 417351.5 55 388741.5 56 352744.5 57 314196.0 58 276536.5 59 238511.5 60 199321.0 61 162815.0 62 130898.5 63 100834.0 64 74422.5 65 54768.0 66 40205.5 67 29604.0 68 21904.5 69 16214.0 70 12196.0 71 9093.5 72 6762.0 73 5148.5 74 3952.0 75 2751.0 76 1819.0 77 1208.0 78 771.0 79 490.5 80 338.0 81 248.5 82 200.0 83 163.5 84 110.0 85 72.5 86 52.5 87 37.5 88 28.5 89 18.5 90 14.0 91 11.0 92 6.0 93 3.0 94 6.5 95 7.5 96 5.0 97 3.5 98 4.5 99 5.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003472307392861 2 0.06520133170952525 3 7.087966925794242E-4 4 7.00832684797633E-4 5 0.0016963336575215435 6 6.689766536704679E-4 7 0.0 8 9.397529182513715E-4 9 2.3892023345373853E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.649946497795722E-4 18-19 0.0010671770427600322 20-21 0.0030541969843169573 22-23 7.964007781791283E-6 24-25 1.5928015563582565E-5 26-27 1.8317217898119954E-4 28-29 9.87536964942119E-4 30-31 0.0015171434824312396 32-33 0.0027436006808270975 34-35 0.003770957684678173 36-37 0.001110979085559884 38-39 0.001322025291777353 40-41 0.004905828793583431 42-43 0.00642297227601467 44-45 0.004643016536784318 46-47 0.006271656128160635 48-49 0.008282568093062935 50-51 0.008071521886845467 52-53 0.0037749396885690687 54-55 0.0020865700388293163 56-57 0.0026082125485366453 58-59 0.003090035019335018 60-61 0.007048146886885286 62-63 0.013487047178463538 64-65 0.00369131760686026 66-67 0.009839531614403131 68-69 0.012165021886686186 70-71 0.005323939202127473 72-73 0.007115840953030512 74-75 0.002847132781990384 76-77 0.0029427008753718795 78-79 0.007390599221502311 80-81 0.005538967412235838 82-83 0.015991727625836897 84-85 0.017254022859250817 86-87 0.02214790564116156 88-89 0.020794024318257043 90-91 0.02390396935704654 92-93 0.03389083511541281 94-95 0.03757418871449128 96-97 0.022617782100287247 98-99 0.01782743141953979 100 0.017409321010995744 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.2556492E7 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.06501736451615 #Duplication Level Percentage of deduplicated Percentage of total 1 68.84758948090966 35.84550941828641 2 17.387791343762927 18.10591316487201 3 6.30706719147503 9.851326885299526 4 2.810833328570061 5.853843442430438 5 1.4918084572857753 3.8835516616557966 6 0.8674159525839217 2.7097215980124116 7 0.5185544189919565 1.8898981380483963 8 0.35140221930189763 1.463661011990625 9 0.2554614664017829 1.1970545115756226 >10 1.0725724775842083 9.367203279611315 >50 0.049716288677933505 1.772628259153829 >100 0.03436351492316562 3.569859259341234 >500 0.0036592865736688822 1.3276853030682207 >1k 0.0016088753471657942 1.4908208032459704 >5k 7.784880537385096E-5 0.24947442329135774 >10k+ 7.784880537385096E-5 1.4218488401169345 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 42467 0.33820751846933045 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 36650 0.29188088520265054 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 26122 0.20803581127595192 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 3.982003890895642E-5 2 0.0 0.0 0.0 7.964007781791284E-6 3.982003890895642E-5 3 0.0 0.0 0.0 7.964007781791284E-6 3.982003890895642E-5 4 0.0 0.0 0.0 1.592801556358257E-5 3.982003890895642E-5 5 0.0 0.0 0.0 1.592801556358257E-5 3.982003890895642E-5 6 9.556809338149541E-5 0.0 0.0 1.592801556358257E-5 9.556809338149541E-5 7 9.556809338149541E-5 0.0 0.0 1.592801556358257E-5 9.556809338149541E-5 8 9.556809338149541E-5 0.0 0.0 1.592801556358257E-5 9.556809338149541E-5 9 9.556809338149541E-5 0.0 0.0 1.592801556358257E-5 1.1946011672686926E-4 10-11 9.556809338149541E-5 0.0 0.0 1.991001945447821E-5 1.314061283995562E-4 12-13 9.556809338149541E-5 7.964007781791284E-6 0.0 3.185603112716514E-5 1.433521400722431E-4 14-15 9.556809338149541E-5 3.185603112716514E-5 0.0 4.7784046690747706E-5 1.9511819065388643E-4 16-17 9.556809338149541E-5 3.982003890895642E-5 0.0 5.973005836343463E-5 2.0706420232657338E-4 18-19 9.556809338149541E-5 3.982003890895642E-5 0.0 7.964007781791283E-5 2.1104620621746902E-4 20-21 9.556809338149541E-5 3.982003890895642E-5 0.0 1.154781128359736E-4 2.1502821010836465E-4 22-23 9.556809338149541E-5 3.982003890895642E-5 0.0 1.9511819065388643E-4 2.2299221789015593E-4 24-25 9.556809338149541E-5 6.371206225433027E-5 0.0 3.0661429959896443E-4 2.3095622567194724E-4 26-27 9.556809338149541E-5 8.760408559970412E-5 0.0 5.296065174891203E-4 2.3892023345373851E-4 28-29 9.556809338149541E-5 8.760408559970412E-5 0.0 0.001835703793702891 2.4290223734463415E-4 30-31 9.955009727239105E-5 1.0353210116328669E-4 0.0 0.005837617704053011 2.468842412355298E-4 32-33 1.0353210116328669E-4 1.1149610894507797E-4 0.0 0.014725450388532083 2.946682879262775E-4 34-35 1.0353210116328669E-4 1.3937013618134746E-4 0.0 0.02872617606892116 3.185603112716513E-4 36-37 1.0353210116328669E-4 1.6326215952672132E-4 0.0 0.05250272130145904 3.5439834628971215E-4 38-39 1.0353210116328669E-4 1.8317217898119952E-4 0.0 0.08849207246737385 3.902363813077729E-4 40-41 1.0353210116328669E-4 1.8317217898119952E-4 0.0 0.1283638774269119 3.902363813077729E-4 42-43 1.0353210116328669E-4 2.0706420232657338E-4 0.0 0.16842283656932205 4.380204279985206E-4 44-45 1.1149610894507797E-4 2.1502821010836465E-4 0.0 0.21804656905766356 4.380204279985206E-4 46-47 1.1946011672686926E-4 2.190102139992603E-4 0.0 0.2795565831603285 4.420024318894162E-4 48-49 1.1946011672686926E-4 2.2299221789015593E-4 0.0 0.3470316390915552 4.4996643967120753E-4 50-51 1.234421206177649E-4 2.2299221789015593E-4 0.0 0.4207664051392698 4.539484435621032E-4 52-53 1.2742412450866055E-4 2.2299221789015593E-4 0.0 0.4945609012453478 4.619124513438945E-4 54-55 1.314061283995562E-4 2.2299221789015593E-4 0.0 0.578039630814084 4.6987645912568575E-4 56-57 1.3937013618134746E-4 2.2299221789015593E-4 0.0 0.6785016069774903 4.738584630165814E-4 58-59 1.433521400722431E-4 2.269742217810516E-4 0.0 0.7930758049302304 4.7784046690747703E-4 60-61 1.433521400722431E-4 2.4290223734463415E-4 0.0 0.9165099615402137 4.7784046690747703E-4 62-63 1.433521400722431E-4 2.468842412355298E-4 0.0 1.0426638268076784 4.858044746892683E-4 64-65 1.433521400722431E-4 2.5086624512642543E-4 0.0 1.174356659487379 4.858044746892683E-4 66-67 1.433521400722431E-4 2.628122567991124E-4 0.0 1.3270943827304633 4.89786478580164E-4 68-69 1.433521400722431E-4 2.628122567991124E-4 0.0 1.5064995860308756 4.977504863619552E-4 70-71 1.433521400722431E-4 2.628122567991124E-4 0.0 1.7039472489609357 5.017324902528509E-4 72-73 1.433521400722431E-4 2.628122567991124E-4 0.0 1.9061096044978167 5.216425097073291E-4 74-75 1.433521400722431E-4 2.628122567991124E-4 0.0 2.108120643886844 5.415525291618073E-4 76-77 1.433521400722431E-4 2.7077626458090365E-4 0.0 2.330969509636927 5.734085602889724E-4 78-79 1.433521400722431E-4 2.7077626458090365E-4 0.0 2.588684801455693 5.853545719616594E-4 80-81 1.433521400722431E-4 2.7077626458090365E-4 0.0 2.872318956600299 5.973005836343462E-4 82-83 1.433521400722431E-4 2.747582684717993E-4 0.0 3.1695755470556586 6.251746108706157E-4 84-85 1.433521400722431E-4 2.7874027236269493E-4 0.0 3.4656693923748767 6.45084630325094E-4 86-87 1.5131614785403438E-4 2.7874027236269493E-4 0.0 3.7749635805924138 6.610126458886765E-4 88 1.5131614785403438E-4 2.7874027236269493E-4 0.0 4.024547620465971 6.610126458886765E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 20595 0.0 46.216373 1 GTATCAA 35395 0.0 37.454185 1 TACCTGG 10380 0.0 30.126879 2 TCAACGC 42920 0.0 30.098911 4 CAACGCA 43580 0.0 29.66405 5 ATCAACG 43825 0.0 29.445307 3 AACGCAG 45295 0.0 28.581587 6 TATCAAC 46215 0.0 28.14486 2 GTACATG 44845 0.0 27.202143 1 GTACCTG 12165 0.0 26.441807 1 TACATGG 45825 0.0 26.024155 2 ACATGGG 45640 0.0 25.216259 3 ACGCAGA 52260 0.0 24.57443 7 CGCAGAG 53700 0.0 23.819195 8 CATGGGG 24955 0.0 22.917582 4 ACCTGGG 13580 0.0 22.389376 3 GCAGAGT 59900 0.0 20.937824 9 GAGTACT 35485 0.0 19.917046 12-13 AGAGTAC 54540 0.0 19.53221 10-11 ATGGGGG 14310 0.0 19.374899 5 >>END_MODULE