FastQCFastQC Report
Mon 6 Jun 2016
ERR1211515_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211515_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9962941
Sequences flagged as poor quality0
Sequence length100
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT302030.30315345639405067No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT271820.2728310847168522No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169320.16994981702692008No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA153200.049.913771
GTATCAA256900.036.052331
TACCTGG85700.033.4140552
GTACCTG96100.030.0841751
TCAACGC300950.029.7454594
CAACGCA303450.029.5295935
ATCAACG304650.029.5086353
AACGCAG317000.028.3650046
TATCAAC325350.027.8209922
GTACATG304150.027.1540661
TACATGG303650.026.3249992
ACCTGGG106850.025.904553
ACATGGG307400.025.0559233
ACGCAGA359550.024.8396117
CGCAGAG364900.024.347048
CATGGGG166000.022.6748524
GCAGAGT413000.020.8427039
GAGTACT266900.020.17545712-13
CAGAGTA403500.019.37664610-11
AGAGTAC379300.018.61231610-11
Produced by FastQC (version 0.11.5)