FastQCFastQC Report
Mon 6 Jun 2016
ERR1211515_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211515_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9962941
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT325650.3268613153485502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT300810.30192891837861935No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT207030.2078000863399673No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA164000.047.624061
GTATCAA278650.035.943571
TACCTGG89700.030.5087662
GTACCTG95700.029.382581
TCAACGC339000.028.8744244
ATCAACG341250.028.615193
CAACGCA344100.028.4737825
AACGCAG357550.027.5732576
TATCAAC363450.027.0522542
GTACATG305000.024.7597561
ACCTGGG106450.024.1912693
ACGCAGA411100.023.8557017
TACATGG309750.023.620682
CGCAGAG417150.023.2956858
ACATGGG308700.022.9099223
TAACGCA11550.021.9702764
GCAGAGT464350.020.5430839
ATAACGC12000.020.3631923
TATAACG11400.020.2108862
GAGTACT297200.019.74084312-13
Produced by FastQC (version 0.11.5)