##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211515_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 9962941 Sequences flagged as poor quality 0 Sequence length 100 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.063901813731505 33.0 31.0 34.0 30.0 34.0 2 32.2875476227351 34.0 31.0 34.0 30.0 34.0 3 32.303621189767156 34.0 31.0 34.0 30.0 34.0 4 35.95686504617462 37.0 35.0 37.0 35.0 37.0 5 35.87357407817631 37.0 35.0 37.0 35.0 37.0 6 35.89038919331149 37.0 35.0 37.0 35.0 37.0 7 35.810821924971755 37.0 35.0 37.0 35.0 37.0 8 35.80249054972824 37.0 35.0 37.0 35.0 37.0 9 37.45010875804645 39.0 37.0 39.0 34.0 39.0 10-11 37.406274362158726 39.0 37.0 39.0 34.0 39.0 12-13 37.31952974528305 39.0 37.0 39.0 34.0 39.0 14-15 38.542034575934956 40.0 38.0 41.0 34.0 41.0 16-17 38.46665061049744 40.0 38.0 41.0 34.0 41.0 18-19 38.45306601735371 40.0 38.0 41.0 34.0 41.0 20-21 38.39132004294716 40.0 38.0 41.0 34.0 41.0 22-23 38.284650235307026 40.0 38.0 41.0 34.0 41.0 24-25 38.17611837709367 40.0 38.0 41.0 33.0 41.0 26-27 37.9960258220941 40.0 37.0 41.0 33.0 41.0 28-29 38.03425333945067 40.0 37.0 41.0 33.0 41.0 30-31 37.96024286402981 40.0 37.0 41.0 33.0 41.0 32-33 37.77963785994517 40.0 37.0 41.0 33.0 41.0 34-35 37.66314028156947 40.0 37.0 41.0 33.0 41.0 36-37 37.60611791237146 40.0 37.0 41.0 32.5 41.0 38-39 37.5123877577916 40.0 37.0 41.0 32.0 41.0 40-41 37.352433834547455 40.0 36.0 41.0 31.0 41.0 42-43 37.201304514399915 40.0 36.0 41.0 31.0 41.0 44-45 37.01909958113774 39.0 36.0 41.0 30.5 41.0 46-47 36.770561473765625 39.0 35.5 41.0 30.0 41.0 48-49 36.55758766412448 39.0 35.0 41.0 30.0 41.0 50-51 36.359553368829545 39.0 35.0 40.0 29.0 41.0 52-53 36.16364976968146 39.0 35.0 40.0 28.5 41.0 54-55 35.96998070148162 38.5 35.0 40.0 28.0 41.0 56-57 35.77204256253249 38.0 34.5 40.0 28.0 41.0 58-59 35.51692431983689 38.0 34.0 40.0 27.0 41.0 60-61 35.20331586827625 38.0 34.0 40.0 26.0 41.0 62-63 34.88213239444056 37.5 33.5 40.0 26.0 41.0 64-65 34.56930644274617 37.0 33.0 40.0 26.0 41.0 66-67 34.23298757866779 36.5 33.0 39.0 25.5 41.0 68-69 33.83266411996217 36.0 32.0 39.0 25.0 40.0 70-71 33.44779061724846 35.5 32.0 38.5 24.0 40.0 72-73 33.03631974735171 35.0 31.0 38.0 23.5 40.0 74-75 32.625543150360926 35.0 31.0 37.0 22.5 39.0 76-77 31.072482211828817 33.5 29.5 35.5 21.0 38.5 78-79 31.91952938394396 34.0 31.0 36.0 22.0 39.0 80-81 31.887216234643965 34.0 31.0 36.0 22.0 38.0 82-83 31.594472555844703 34.0 31.0 35.5 21.5 37.0 84-85 31.28296248065707 34.0 30.0 35.0 20.5 37.0 86-87 30.977325470460983 34.0 30.0 35.0 20.0 36.0 88-89 30.688288628829582 34.0 30.0 35.0 20.0 36.0 90-91 30.393542830375086 34.0 29.0 35.0 19.0 35.5 92-93 30.177010633707454 34.0 29.0 35.0 18.5 35.0 94-95 29.948519468297565 34.0 29.0 35.0 18.0 35.0 96-97 29.740789291033643 34.0 29.0 35.0 18.0 35.0 98-99 29.568432152714745 33.5 29.0 35.0 17.5 35.0 100 27.689183946788404 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3366098699364599 1101 2 0.25138947071426543 1101 3 0.2979474402951041 1101 4 0.19845232955539416 1101 5 0.2258628068690669 1101 6 0.2402799330757972 1101 7 0.2564911913670542 1101 8 0.19918416822031304 1101 9 0.27464623216704354 1101 10-11 0.2219815229617339 1101 12-13 0.19086833473719622 1101 14-15 0.3153993340312411 1101 16-17 0.3260385988821355 1101 18-19 0.34415680350128497 1101 20-21 0.3437172056122648 1101 22-23 0.2966753164524505 1101 24-25 0.276122618372483 1101 26-27 0.23799069832899278 1101 28-29 0.32619759871491993 1101 30-31 0.3828787259729367 1101 32-33 0.4364830591614748 1101 34-35 0.3902616236238359 1101 36-37 0.446388675755351 1101 38-39 0.367142392814479 1101 40-41 0.42573771203465327 1101 42-43 0.42039179490480905 1101 44-45 0.40172713493163315 1101 46-47 0.38640657111073295 1101 48-49 0.3792495110804097 1101 50-51 0.4378213496293526 1101 52-53 0.43067927883426904 1101 54-55 0.36824832622173176 1101 56-57 0.3896747417774904 1101 58-59 0.3533070684737467 1101 60-61 0.36684750199415816 1101 62-63 0.3294798291657699 1101 64-65 0.38869178875112453 1101 66-67 0.29348059227442036 1101 68-69 0.29386775777027907 1101 70-71 0.3200957304121914 1101 72-73 0.20008262981922087 1101 74-75 0.28715088690209 1101 76-77 0.30923540900119306 1101 78-79 0.4028206075982155 1101 80-81 0.31138765948316305 1101 82-83 0.27584566818078926 1101 84-85 0.18735202693225972 1101 86-87 0.17457396403079528 1101 88-89 0.1666327325350636 1101 90-91 0.21521263741519547 1101 92-93 0.12146388015227672 1101 94-95 0.11280301353131961 1101 96-97 0.0850379664937222 1101 98-99 0.0849165149372304 1101 100 0.11734143125017837 1102 1 0.31152222771954996 1102 2 0.20285265244240946 1102 3 0.2980432811513367 1102 4 0.2060024295744327 1102 5 0.20491199569632101 1102 6 0.18260911725222684 1102 7 0.17964526493342703 1102 8 0.17213705363182186 1102 9 0.258719277360548 1102 10-11 0.21737197812475273 1102 12-13 0.23884539388163262 1102 14-15 0.3415328911517719 1102 16-17 0.36512362830745815 1102 18-19 0.29706886847235836 1102 20-21 0.34048860619608945 1102 22-23 0.28360275711143146 1102 24-25 0.2669295391790172 1102 26-27 0.2901188433284929 1102 28-29 0.3475237906184958 1102 30-31 0.36998825212700126 1102 32-33 0.40651949518603203 1102 34-35 0.3662760299360741 1102 36-37 0.4483217736938272 1102 38-39 0.3749787779633067 1102 40-41 0.3571631790513692 1102 42-43 0.30935395149973743 1102 44-45 0.3707313285392928 1102 46-47 0.3626921706508526 1102 48-49 0.40627520099142345 1102 50-51 0.4143106291866019 1102 52-53 0.45459931252366914 1102 54-55 0.4012790766270058 1102 56-57 0.4286504034409333 1102 58-59 0.5057263974052901 1102 60-61 0.42011826410802655 1102 62-63 0.4835677352725938 1102 64-65 0.4659911637522711 1102 66-67 0.43303520307368615 1102 68-69 0.45537757978385685 1102 70-71 0.4216442978510173 1102 72-73 0.40140721839019733 1102 74-75 0.3611645825847134 1102 76-77 0.3801455593873868 1102 78-79 0.37942296723406344 1102 80-81 0.35203610804049745 1102 82-83 0.34726834877626445 1102 84-85 0.2620382463953028 1102 86-87 0.27198002276459476 1102 88-89 0.3164865958532914 1102 90-91 0.26117582540332407 1102 92-93 0.20217941148573715 1102 94-95 0.23688175239958298 1102 96-97 0.2869906523621424 1102 98-99 0.26389846473137624 1102 100 0.25079814937392086 1103 1 0.2826127950474273 1103 2 0.258015593812992 1103 3 0.29639741139772013 1103 4 0.17472649606317248 1103 5 0.19264462210010436 1103 6 0.20310143142396697 1103 7 0.21024838299978654 1103 8 0.23480363001485216 1103 9 0.26406947417429905 1103 10-11 0.25502266393714734 1103 12-13 0.28817246642528005 1103 14-15 0.41029517883796984 1103 16-17 0.3727091762095398 1103 18-19 0.36732900217837994 1103 20-21 0.3262404967867738 1103 22-23 0.371756611607303 1103 24-25 0.3676897163286128 1103 26-27 0.3667918854942016 1103 28-29 0.432335946091456 1103 30-31 0.45343214557949807 1103 32-33 0.5157153991898582 1103 34-35 0.48940413492660184 1103 36-37 0.4976240591875083 1103 38-39 0.4941412801526468 1103 40-41 0.5608653839160738 1103 42-43 0.5518444690078184 1103 44-45 0.5566451395101666 1103 46-47 0.6532810794022978 1103 48-49 0.6180811452246999 1103 50-51 0.6667560619654864 1103 52-53 0.6473924813572225 1103 54-55 0.6578753285600811 1103 56-57 0.7018596637382686 1103 58-59 0.6336875917248577 1103 60-61 0.6770953911048991 1103 62-63 0.6965805125512148 1103 64-65 0.696778749019785 1103 66-67 0.6989808941895035 1103 68-69 0.7572954109299914 1103 70-71 0.7872159718944118 1103 72-73 0.6902839538359871 1103 74-75 0.6293432400289518 1103 76-77 0.6546800476286307 1103 78-79 0.6250697987926657 1103 80-81 0.6527009766945504 1103 82-83 0.7128531269549292 1103 84-85 0.6080540099473701 1103 86-87 0.6369368021227331 1103 88-89 0.6836198179122803 1103 90-91 0.6838464924926946 1103 92-93 0.6031146300127261 1103 94-95 0.6455764858596638 1103 96-97 0.7024681849970378 1103 98-99 0.7232641311213364 1103 100 0.7600356373275723 1104 1 0.25920254671688525 1104 2 0.23508832891653242 1104 3 0.2851531215536838 1104 4 0.10469768821484848 1104 5 0.1383058354017095 1104 6 0.18990290181605474 1104 7 0.181516988345102 1104 8 0.18003074484533954 1104 9 0.23025899221949686 1104 10-11 0.27858954934531255 1104 12-13 0.2848750127235036 1104 14-15 0.35776985528048044 1104 16-17 0.33895412135348835 1104 18-19 0.3032965325204344 1104 20-21 0.25830525688194683 1104 22-23 0.2882640158927856 1104 24-25 0.3015850794776185 1104 26-27 0.3893136828163719 1104 28-29 0.4766604610361398 1104 30-31 0.3539870345694709 1104 32-33 0.3783643326611923 1104 34-35 0.4474341853461965 1104 36-37 0.3163785446846177 1104 38-39 0.4595729967793787 1104 40-41 0.5448893676272633 1104 42-43 0.569521452670898 1104 44-45 0.5133134479873647 1104 46-47 0.5266673596165532 1104 48-49 0.5470600812614705 1104 50-51 0.4692925807830264 1104 52-53 0.5127274805935329 1104 54-55 0.5225610027142551 1104 56-57 0.5959614962098172 1104 58-59 0.5286126711134287 1104 60-61 0.52070369185369 1104 62-63 0.5446082674295099 1104 64-65 0.6460778484911742 1104 66-67 0.6482413322938925 1104 68-69 0.6376077851191084 1104 70-71 0.5574416062951002 1104 72-73 0.6803870784155563 1104 74-75 0.7623042316873452 1104 76-77 0.615777575157562 1104 78-79 0.6681918764224264 1104 80-81 0.738729555302573 1104 82-83 0.5983217904745608 1104 84-85 0.6187189224525795 1104 86-87 0.6952324704973769 1104 88-89 0.661595416035496 1104 90-91 0.48395849820265013 1104 92-93 0.5241514856295524 1104 94-95 0.6118982226417309 1104 96-97 0.5775721741765878 1104 98-99 0.6601396664569457 1104 100 0.821614202548357 1105 1 0.2473671028619222 1105 2 0.16424209795102485 1105 3 0.18252832970145505 1105 4 0.10955038198052591 1105 5 0.08302006517963889 1105 6 0.13224456966599263 1105 7 0.20596857543155522 1105 8 0.19528794449939824 1105 9 0.1492256326391086 1105 10-11 0.19131627444395605 1105 12-13 0.22373219000301958 1105 14-15 0.2905836689780017 1105 16-17 0.31779262449668266 1105 18-19 0.3235799146905052 1105 20-21 0.23455313237622732 1105 22-23 0.3050868310403487 1105 24-25 0.34361574120467253 1105 26-27 0.34210710125901755 1105 28-29 0.3908959186600498 1105 30-31 0.3973592221541793 1105 32-33 0.45900192102986637 1105 34-35 0.5193247910508347 1105 36-37 0.5223836133787643 1105 38-39 0.5109707601858204 1105 40-41 0.4868621137149489 1105 42-43 0.46121361340615863 1105 44-45 0.506831543397567 1105 46-47 0.5611807778870883 1105 48-49 0.5375615721804152 1105 50-51 0.5554747525764654 1105 52-53 0.5305369269527276 1105 54-55 0.46608628379442507 1105 56-57 0.47592819256578167 1105 58-59 0.5626675834009234 1105 60-61 0.5908998563409966 1105 62-63 0.5375335235534635 1105 64-65 0.5620390716901298 1105 66-67 0.5448390251344648 1105 68-69 0.5981022624799408 1105 70-71 0.6430401467865607 1105 72-73 0.6239281077305137 1105 74-75 0.6003377676823902 1105 76-77 0.585953602557769 1105 78-79 0.7333653866867422 1105 80-81 0.7094714864836469 1105 82-83 0.6741275966680114 1105 84-85 0.7003542432475633 1105 86-87 0.6942472161805959 1105 88-89 0.6683689641644222 1105 90-91 0.7006065337609577 1105 92-93 0.6898078557044656 1105 94-95 0.7610853813761942 1105 96-97 0.7487145235894701 1105 98-99 0.8723954074630278 1105 100 0.8888125227366466 1106 1 0.2951408291748763 1106 2 0.24011421626875773 1106 3 0.2507454483246079 1106 4 0.1805736822663775 1106 5 0.18922466539979155 1106 6 0.19559127906626372 1106 7 0.19640295479076997 1106 8 0.20038299186413866 1106 9 0.182175061631888 1106 10-11 0.20276570156682538 1106 12-13 0.25697781223945526 1106 14-15 0.3107750851083182 1106 16-17 0.35276612791673756 1106 18-19 0.35398397662680736 1106 20-21 0.35284813885233746 1106 22-23 0.3453306170282602 1106 24-25 0.3589837700361471 1106 26-27 0.32370607106501126 1106 28-29 0.4052498346812854 1106 30-31 0.3941654212693422 1106 32-33 0.4291510024692968 1106 34-35 0.39183908025561465 1106 36-37 0.4301315225112603 1106 38-39 0.3418911034009895 1106 40-41 0.382255697939911 1106 42-43 0.37005752480249754 1106 44-45 0.4607753389843481 1106 46-47 0.4972165379855227 1106 48-49 0.519259784175496 1106 50-51 0.5494424580387189 1106 52-53 0.5699724768736232 1106 54-55 0.46673282278385386 1106 56-57 0.5426471044295909 1106 58-59 0.6123401627130178 1106 60-61 0.6744940292126316 1106 62-63 0.6430830206108524 1106 64-65 0.7295361639849958 1106 66-67 0.7055920989496656 1106 68-69 0.6236220280185591 1106 70-71 0.6599077661084323 1106 72-73 0.6428601291372118 1106 74-75 0.6011927264883425 1106 76-77 0.6268657306488805 1106 78-79 0.6446502620075876 1106 80-81 0.6968071185557854 1106 82-83 0.716588693256174 1106 84-85 0.6673916625766658 1106 86-87 0.6246057271862426 1106 88-89 0.6834618050390908 1106 90-91 0.6985110662904823 1106 92-93 0.6687032768012529 1106 94-95 0.7996695627122783 1106 96-97 0.8277362132622272 1106 98-99 0.801721231250486 1106 100 0.9337633130216076 1107 1 0.1827337265318505 1107 2 0.17212505861472494 1107 3 0.22883508430862776 1107 4 0.11899772511678464 1107 5 0.1261853825557111 1107 6 0.13175315455730896 1107 7 0.17347507018613584 1107 8 0.14616963980663655 1107 9 0.20659754779969575 1107 10-11 0.1713862257954375 1107 12-13 0.183681725515477 1107 14-15 0.23289970605546273 1107 16-17 0.20902782915555917 1107 18-19 0.18725132888808105 1107 20-21 0.24846572563724578 1107 22-23 0.3036520212359264 1107 24-25 0.30998844911118795 1107 26-27 0.3220653193259295 1107 28-29 0.32867059285104006 1107 30-31 0.21758541372245332 1107 32-33 0.27220955077778797 1107 34-35 0.29874662944341424 1107 36-37 0.3414203199489876 1107 38-39 0.3360251717617828 1107 40-41 0.3615300892132538 1107 42-43 0.32799142259423064 1107 44-45 0.3774153484573759 1107 46-47 0.36548453333110587 1107 48-49 0.4460979668318501 1107 50-51 0.36829114733606616 1107 52-53 0.40512695083494776 1107 54-55 0.34404481846047474 1107 56-57 0.41591477558537093 1107 58-59 0.48341060953533344 1107 60-61 0.5016895132427948 1107 62-63 0.4851339454914907 1107 64-65 0.5674141012542933 1107 66-67 0.597119477051649 1107 68-69 0.5226990780584515 1107 70-71 0.6188933055285801 1107 72-73 0.5801879153871781 1107 74-75 0.5883775842878691 1107 76-77 0.4588558784728747 1107 78-79 0.6669999801137578 1107 80-81 0.594612906657499 1107 82-83 0.5904478868242542 1107 84-85 0.4880272916125534 1107 86-87 0.46559612610814227 1107 88-89 0.47776258529510685 1107 90-91 0.35561597453081717 1107 92-93 0.4061413548259125 1107 94-95 0.5780980875686623 1107 96-97 0.6590569894141325 1107 98-99 0.7291740591126619 1107 100 1.024205938764343 1108 1 0.2586659551613053 1108 2 0.21748785687606187 1108 3 0.2613027161011985 1108 4 0.15852153499348987 1108 5 0.15968776714590405 1108 6 0.15776608382973478 1108 7 0.14298549239167357 1108 8 0.17313778571572414 1108 9 0.177164338786298 1108 10-11 0.18662260477615433 1108 12-13 0.23742457676377882 1108 14-15 0.2681780443578745 1108 16-17 0.278648457072066 1108 18-19 0.2885146465633426 1108 20-21 0.319329218478579 1108 22-23 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76-77 -0.39985447115009265 2316 78-79 -0.32262478282154916 2316 80-81 -0.281822852913038 2316 82-83 -0.15386782652997155 2316 84-85 -0.17060007724833426 2316 86-87 -0.25480646882191493 2316 88-89 -0.1259943448790537 2316 90-91 -0.06515963799668256 2316 92-93 -0.02094854023316728 2316 94-95 0.05525921394021793 2316 96-97 0.028689543831013964 2316 98-99 -0.10616953860725076 2316 100 -0.19508387700197005 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 6.0 10 24.0 11 47.0 12 121.0 13 307.0 14 995.0 15 2724.0 16 6132.0 17 12725.0 18 22583.0 19 34191.0 20 47576.0 21 64411.0 22 82765.0 23 93557.0 24 99545.0 25 111849.0 26 131119.0 27 158794.0 28 191357.0 29 231128.0 30 277703.0 31 335324.0 32 406850.0 33 508756.0 34 662227.0 35 897496.0 36 1299853.0 37 1999229.0 38 1983978.0 39 299527.0 40 42.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 41.87103531054137 20.275019638380773 13.498779539627773 24.355165511450082 2 14.916432172154877 21.557505566953445 39.77456788881018 23.7514943720815 3 17.10827816932616 26.718554006592683 31.06287577662133 25.110292047459833 4 11.61910384673369 17.68044505943755 38.62936813339578 32.07108296043298 5 12.091726057624786 38.4186025689977 36.04681858385939 13.442852789518122 6 30.46044532415658 38.59902608238301 17.916571103363445 13.023957490096965 7 26.47141039980062 32.973064881143024 22.96593947510078 17.589585243955575 8 25.234406099948632 36.74478095498491 20.453920860702542 17.566892084363914 9 25.96368883445159 15.07341055216527 20.598526077791686 38.36437453559145 10-11 24.04318664538915 26.65286786301354 29.586374143940024 19.717571347657284 12-13 24.64766678835095 24.518718920447284 29.313738784561703 21.51987550664006 14-15 21.973822789877005 26.05789796406503 26.84593334438094 25.122345901677022 16-17 21.55732696569187 28.856537108148277 27.585563300142606 22.000572626017245 18-19 21.318304886874873 28.008265113575305 29.4960738853617 21.17735611418813 20-21 22.112648050349616 27.27907050508007 29.509393978996368 21.098887465573945 22-23 21.805263124613504 27.293878383902904 29.190055426404715 21.710803065078878 24-25 21.602815032510485 27.386860705775607 29.395298978012512 21.615025283701396 26-27 21.629591711269534 27.514093501657396 29.306047169882337 21.550267617190734 28-29 21.576137216635267 27.482407258288532 29.48700042442698 21.454455100649216 30-31 21.903034810108 27.365886725509338 29.43014458361854 21.300933880764124 32-33 21.594753047090794 27.532390074396307 29.383070793804684 21.48978608470821 34-35 21.879937863347948 27.568421684470994 29.339688662289344 21.21195178989171 36-37 21.889245007721467 27.506058656841866 29.16600692144049 21.43868941399618 38-39 21.744702461218147 27.714845123271186 29.208063327110356 21.332389088400305 40-41 21.910717949479785 27.553258101338347 29.23528782341012 21.30073612577175 42-43 21.68645613201065 27.562792559579947 29.140954280879484 21.609797027529922 44-45 21.777595805281912 27.77279266155202 28.96081062925037 21.488800903915696 46-47 21.727857268115244 27.67073464155369 28.778803501645754 21.82260458868531 48-49 21.71190965115076 27.737282622323395 28.815629881941522 21.735177844584328 50-51 21.780641405123664 27.917005554795733 28.591194889638118 21.71115815044249 52-53 21.80911871225183 28.00101560149797 28.441680052604905 21.7481856336453 54-55 21.76700105468106 27.94220759325195 28.555779529250398 21.735011822816595 56-57 21.83799601172093 28.007005736300417 28.360148204908047 21.794850047070607 58-59 21.7961308594742 27.93381483958145 28.559533400129645 21.710520900814707 60-61 21.93536963293627 28.08654315137157 28.423425860558606 21.554661355133558 62-63 21.75388102475547 28.024821171628805 28.56600057229897 21.65529723131675 64-65 21.728975034862984 27.840990475050976 28.51373334980833 21.916301140277717 66-67 21.6679184288708 27.984137854051955 28.353116596780794 21.994827120296456 68-69 21.65160466393728 27.95793032362309 28.429781949126813 21.96068306331281 70-71 21.638228510528204 27.776006954142357 28.464695908092498 22.12106862723694 72-73 21.60150914214267 27.933353382420133 28.278241507712742 22.18689596772445 74-75 21.590610019691525 27.97808457217559 28.192826375119424 22.238479033013466 76-77 21.47018109727154 27.89098831767469 28.383035770477694 22.25579481457607 78-79 21.488507633279177 27.81578505830966 28.333125631543517 22.36258167686764 80-81 21.437604700163426 28.045831833105694 28.09561914792022 22.420944318810662 82-83 21.558087670351934 27.986563837657563 28.142552098859525 22.312796393130977 84-85 21.49895371137191 27.954225016831625 28.091814393331543 22.455006878464925 86-87 21.37945518992364 27.93076651586904 28.245975270514357 22.44380302369296 88-89 21.381979314689083 27.827115724668307 28.2239699446929 22.566935015949717 90-91 21.498898757359772 27.856233270278015 28.12629824449735 22.51856972786486 92-93 21.377983288823344 27.82163512813114 28.107937879195894 22.692443703849627 94-95 21.220441230883853 27.715124279747787 28.17528241517755 22.88915207419081 96-97 21.21368889485012 27.673959893011084 28.22156427818323 22.890786933955567 98-99 21.15485584593262 27.60257653033566 28.091094520430776 23.151473103300944 100 20.365342671512444 28.07717088671006 28.305297981731766 23.252188460045733 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 3785.0 1 2701.5 2 2802.0 3 3997.0 4 3094.0 5 2558.5 6 3935.5 7 7389.5 8 11210.0 9 12999.5 10 11407.5 11 9036.5 12 9017.0 13 9687.0 14 10415.0 15 11239.5 16 12101.0 17 13039.0 18 14170.0 19 15688.0 20 17753.0 21 20824.0 22 25191.5 23 31518.5 24 39830.5 25 50016.0 26 63506.5 27 79129.0 28 94920.0 29 112941.5 30 133461.5 31 153248.5 32 175291.5 33 201005.5 34 226490.5 35 249423.0 36 277297.5 37 303234.5 38 319527.0 39 332802.5 40 339689.0 41 343273.0 42 347663.0 43 353362.5 44 360117.5 45 368089.0 46 372932.5 47 372137.5 48 373936.0 49 374669.0 50 369792.5 51 354569.0 52 332494.0 53 314147.0 54 295173.5 55 271517.5 56 243794.0 57 215206.0 58 186621.0 59 157402.5 60 128707.0 61 102703.5 62 80870.5 63 61565.0 64 44829.0 65 32328.5 66 23380.5 67 17419.0 68 12931.5 69 9485.0 70 7181.5 71 5360.5 72 3933.5 73 2897.5 74 2142.5 75 1519.5 76 1050.5 77 706.0 78 466.5 79 315.5 80 219.0 81 148.5 82 104.0 83 77.5 84 58.0 85 37.5 86 27.5 87 21.0 88 13.5 89 11.5 90 10.5 91 5.5 92 4.5 93 4.0 94 2.0 95 2.0 96 3.5 97 4.5 98 3.0 99 4.5 100 5.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0030814194322740644 2 0.0644187293691692 3 7.427525667370709E-4 4 4.71748251846518E-4 5 0.0014553935429307472 6 8.6319892891065E-4 7 0.0 8 0.0010338312753232204 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 6.875479840741806E-4 18-19 0.001018775480051523 20-21 0.0028405267079269062 22-23 0.0 24-25 1.0037196847798255E-5 26-27 1.6059514956477208E-4 28-29 9.685894958125316E-4 30-31 0.0016009328972238215 32-33 0.0028355081095030074 34-35 0.0038442463927067317 36-37 0.0013098541886376724 38-39 0.0013098541886376724 40-41 0.005149081982920505 42-43 0.006564326738460059 44-45 0.0050938774002576145 46-47 0.006564326738460059 48-49 0.008471394139541729 50-51 0.008315817588400855 52-53 0.003733837227380951 54-55 0.0021429415270049273 56-57 0.002489224818253967 58-59 0.00316171700705645 60-61 0.007226781730414743 62-63 0.013695755098820721 64-65 0.0036936884399897583 66-67 0.009886638895081281 68-69 0.012335714925944057 70-71 0.005354844518300369 72-73 0.007276967714653735 74-75 0.0028003779205357134 76-77 0.0028505639047747043 78-79 0.007442581462642406 80-81 0.005660979022158216 82-83 0.016205054310770284 84-85 0.017861191790656997 86-87 0.02248833953749199 88-89 0.021123280766191427 90-91 0.023838342513520856 92-93 0.03391568814871031 94-95 0.03769971136033025 96-97 0.022914920403523418 98-99 0.01799669394810227 100 0.018337958640927413 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 9962941.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 59.76071270482477 #Duplication Level Percentage of deduplicated Percentage of total 1 71.63507649834506 42.809632262057434 2 17.591984489785002 21.026190620035496 3 5.65253962198657 10.133993892065345 4 2.188393727750256 5.231198753964943 5 1.0432297123016332 3.1172075560997445 6 0.5557120952142383 1.9925850521216588 7 0.33824642950118183 1.4149693387797024 8 0.21388587842563747 1.0225578025770878 9 0.14336200949088926 0.7710674275774255 >10 0.5847923483240253 5.902003594485724 >50 0.028593553494007214 1.1938597127257176 >100 0.021587480285261453 2.5440478565654727 >500 0.0016256945700209987 0.6546360802178268 >1k 8.491529634509424E-4 0.812965191347548 >5k 4.852302515100901E-5 0.16461997501015863 >10k+ 7.278453772651352E-5 1.2084648843686552 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32565 0.3268613153485502 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 30081 0.30192891837861935 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 20703 0.2078000863399673 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 1.0037196847798255E-5 0.0 2 0.0 0.0 0.0 1.0037196847798255E-5 0.0 3 0.0 0.0 0.0 1.0037196847798255E-5 0.0 4 0.0 0.0 0.0 1.0037196847798255E-5 1.0037196847798255E-5 5 0.0 1.0037196847798255E-5 0.0 1.0037196847798255E-5 1.0037196847798255E-5 6 4.014878739119302E-5 1.0037196847798255E-5 0.0 1.0037196847798255E-5 2.007439369559651E-5 7 4.014878739119302E-5 2.007439369559651E-5 0.0 1.0037196847798255E-5 3.0111590543394767E-5 8 4.014878739119302E-5 2.007439369559651E-5 0.0 2.007439369559651E-5 4.014878739119302E-5 9 4.014878739119302E-5 2.007439369559651E-5 0.0 2.007439369559651E-5 4.014878739119302E-5 10-11 4.014878739119302E-5 2.007439369559651E-5 0.0 2.007439369559651E-5 4.014878739119302E-5 12-13 4.014878739119302E-5 2.5092992119495637E-5 0.0 2.007439369559651E-5 4.014878739119302E-5 14-15 4.014878739119302E-5 4.014878739119303E-5 0.0 2.007439369559651E-5 5.018598423899128E-5 16-17 8.029757478238604E-5 5.018598423899128E-5 0.0 2.5092992119495637E-5 5.018598423899128E-5 18-19 8.029757478238604E-5 6.0223181086789535E-5 0.0 4.516738581509215E-5 7.527897635848692E-5 20-21 8.029757478238604E-5 8.029757478238604E-5 0.0 6.524177951068867E-5 1.1040916532578081E-4 22-23 8.531617320628516E-5 8.029757478238604E-5 0.0 1.1542776374967993E-4 1.3550215744527645E-4 24-25 9.03347716301843E-5 8.029757478238604E-5 0.0 2.76022913314452E-4 1.6561374798867122E-4 26-27 9.03347716301843E-5 8.029757478238604E-5 0.0 5.319714329333076E-4 1.9070674010816685E-4 28-29 9.03347716301843E-5 8.029757478238604E-5 0.0 0.0017765838420602913 1.9070674010816685E-4 30-31 9.03347716301843E-5 9.03347716301843E-5 0.0 0.005791462581179593 1.9070674010816685E-4 32-33 9.03347716301843E-5 9.03347716301843E-5 0.0 0.014819921145774125 1.9070674010816685E-4 34-35 1.0037196847798256E-4 1.0037196847798255E-4 0.0 0.029218280023940724 2.0074393695596512E-4 36-37 1.0037196847798256E-4 1.1040916532578081E-4 0.0 0.05324732927756975 2.2081833065156164E-4 38-39 1.0037196847798256E-4 1.1040916532578081E-4 0.0 0.08942138671703466 2.459113227710573E-4 40-41 1.0037196847798256E-4 1.1040916532578081E-4 0.0 0.13035307546235594 2.509299211949564E-4 42-43 1.0037196847798256E-4 1.1040916532578081E-4 0.0 0.1708431275463741 2.509299211949564E-4 44-45 1.1040916532578082E-4 1.1040916532578081E-4 0.0 0.22251963551726342 2.509299211949564E-4 46-47 1.2546496059747818E-4 1.1040916532578081E-4 0.0 0.28668743496523763 2.6598571646665374E-4 48-49 1.3048355902137732E-4 1.1040916532578081E-4 0.0 0.35528163822309095 3.262088975534433E-4 50-51 1.3550215744527645E-4 1.1040916532578081E-4 0.0 0.42830726388924717 3.5130188967293893E-4 52-53 1.405207558691756E-4 1.1040916532578081E-4 0.0 0.5016942286419241 3.763948817924346E-4 54-55 1.405207558691756E-4 1.5557655114087295E-4 0.0 0.588420628005325 4.0148787391193025E-4 56-57 1.405207558691756E-4 1.6059514956477208E-4 0.0 0.6930182563562306 4.1654366918362763E-4 58-59 1.405207558691756E-4 1.7063234641257036E-4 0.0 0.8119439832073683 4.366180628792241E-4 60-61 1.5557655114087297E-4 1.7063234641257036E-4 0.0 0.9370375675214778 4.566924565748206E-4 62-63 1.7063234641257036E-4 1.756509448364695E-4 0.0 1.0661159189841634 4.817854486943163E-4 64-65 1.7063234641257036E-4 1.9070674010816685E-4 0.0 1.2002379618628676 4.817854486943163E-4 66-67 1.756509448364695E-4 2.0074393695596512E-4 0.0 1.3591970483414486 4.817854486943163E-4 68-69 1.806695432603686E-4 2.0576253537986426E-4 0.0 1.5431989409552862 4.817854486943163E-4 70-71 1.9070674010816685E-4 2.1078113380376337E-4 0.0 1.7446203886984777 5.018598423899127E-4 72-73 2.2081833065156162E-4 2.1078113380376337E-4 0.0 1.9505736308184503 5.921946140200971E-4 74-75 2.2081833065156162E-4 2.1078113380376337E-4 0.0 2.1581177686387987 5.921946140200971E-4 76-77 2.2081833065156162E-4 2.1078113380376337E-4 0.0 2.3884363061068012 5.972132124439963E-4 78-79 2.2081833065156162E-4 2.2081833065156162E-4 0.0 2.654181129849108 6.022318108678953E-4 80-81 2.2081833065156162E-4 2.3085552749935986E-4 0.0 2.9432122502783065 6.323434014112901E-4 82-83 2.3085552749935986E-4 2.3085552749935986E-4 0.0 3.244182616357961 6.624549919546849E-4 84-85 2.459113227710573E-4 2.3085552749935986E-4 0.0 3.545835511823266 6.624549919546849E-4 86-87 2.509299211949564E-4 2.3085552749935986E-4 0.0 3.8640698564811333 6.674735903785839E-4 88 2.509299211949564E-4 2.3085552749935986E-4 0.0 4.120991984194226 6.724921888024831E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 16400 0.0 47.62406 1 GTATCAA 27865 0.0 35.94357 1 TACCTGG 8970 0.0 30.508766 2 GTACCTG 9570 0.0 29.38258 1 TCAACGC 33900 0.0 28.874424 4 ATCAACG 34125 0.0 28.61519 3 CAACGCA 34410 0.0 28.473782 5 AACGCAG 35755 0.0 27.573257 6 TATCAAC 36345 0.0 27.052254 2 GTACATG 30500 0.0 24.759756 1 ACCTGGG 10645 0.0 24.191269 3 ACGCAGA 41110 0.0 23.855701 7 TACATGG 30975 0.0 23.62068 2 CGCAGAG 41715 0.0 23.295685 8 ACATGGG 30870 0.0 22.909922 3 TAACGCA 1155 0.0 21.970276 4 GCAGAGT 46435 0.0 20.543083 9 ATAACGC 1200 0.0 20.363192 3 TATAACG 1140 0.0 20.210886 2 GAGTACT 29720 0.0 19.740843 12-13 >>END_MODULE