FastQCFastQC Report
Mon 6 Jun 2016
ERR1211514_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211514_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15777071
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT624330.39571983925279924No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT503840.31934951677659307No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT386680.24508985222922558No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA268200.055.4719771
GTATCAA521100.039.3832131
GTACATG745800.033.8556181
TACATGG781750.031.5618062
ATCAACG625250.031.4167793
ACATGGG753500.031.3207763
CAACGCA631350.031.1914525
TCAACGC636450.030.899664
AACGCAG662200.029.9018046
TATCAAC689450.028.7006422
CATGGGG273150.027.6979244
CATGGGA420550.026.4374544
ACGCAGA747800.026.2037227
CGCAGAG751500.025.8695588
TACCTGG89750.023.7870222
ATGGGGG164300.023.1365765
GCAGAGT832350.022.7550819
ATGGGAT220750.021.1366755
GAGTACT482750.020.69839912-13
AGAGTAC792750.020.2881410-11
Produced by FastQC (version 0.11.5)