FastQCFastQC Report
Mon 6 Jun 2016
ERR1211514_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1211514_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15777071
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT677070.4291480972608921No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT585110.3708609792020331No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT447690.28375989434287263No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA292000.054.771641
GTATCAA544450.038.4183541
GTACATG749050.031.484161
ATCAACG679550.029.6388383
TACATGG787650.029.5938242
ACATGGG757050.029.4290223
CAACGCA686250.029.3698235
TCAACGC689750.029.2413274
AACGCAG715700.028.3251826
TATCAAC741500.027.3580062
CATGGGG272900.026.4322364
TACCTGG92350.025.1029912
CATGGGA426250.024.9597994
ACGCAGA804850.024.9013867
CGCAGAG806300.024.7283888
GCAGAGT886450.022.1053359
ATGGGGG159950.022.0345765
GAGTACT510450.021.0362812-13
AGAGTAC844850.020.87467810-11
ATGGGCG22900.020.52075
Produced by FastQC (version 0.11.5)