##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename ERR1211514_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 15777071 Sequences flagged as poor quality 0 Sequence length 100 %GC 41 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.153588584344966 33.0 31.0 34.0 30.0 34.0 2 32.396617471012206 34.0 31.0 34.0 31.0 34.0 3 32.41638140564874 34.0 31.0 34.0 31.0 34.0 4 36.04208271611378 37.0 35.0 37.0 35.0 37.0 5 35.9751618662298 37.0 35.0 37.0 35.0 37.0 6 35.99470117108556 37.0 35.0 37.0 35.0 37.0 7 35.92400851843793 37.0 35.0 37.0 35.0 37.0 8 35.923074251234596 37.0 35.0 37.0 35.0 37.0 9 37.594447410422376 39.0 37.0 39.0 35.0 39.0 10-11 37.56021377478747 39.0 37.0 39.0 35.0 39.0 12-13 37.49024505245619 39.0 37.0 39.0 35.0 39.0 14-15 38.71943524878604 40.0 38.0 41.0 34.5 41.0 16-17 38.667585954325745 40.0 38.0 41.0 34.0 41.0 18-19 38.68959390497767 40.0 38.0 41.0 34.0 41.0 20-21 38.643632458775144 40.0 38.0 41.0 34.0 41.0 22-23 38.53615873947706 40.0 38.0 41.0 34.0 41.0 24-25 38.438939331641464 40.0 38.0 41.0 34.0 41.0 26-27 38.268450588832366 40.0 38.0 41.0 34.0 41.0 28-29 38.309942352417636 40.0 38.0 41.0 34.0 41.0 30-31 38.23001886725362 40.0 38.0 41.0 34.0 41.0 32-33 38.05918642947097 40.0 37.0 41.0 33.5 41.0 34-35 37.961089482325335 40.0 37.0 41.0 33.0 41.0 36-37 37.89055756927252 40.0 37.0 41.0 33.0 41.0 38-39 37.80605677695182 40.0 37.0 41.0 33.0 41.0 40-41 37.66352832537801 40.0 37.0 41.0 33.0 41.0 42-43 37.525479095581176 40.0 37.0 41.0 32.0 41.0 44-45 37.34629006866991 40.0 37.0 41.0 31.5 41.0 46-47 37.09106481171315 40.0 36.0 41.0 31.0 41.0 48-49 36.866419818989215 39.0 36.0 41.0 30.0 41.0 50-51 36.685153790586355 39.0 36.0 41.0 30.0 41.0 52-53 36.52068935989449 39.0 35.5 40.5 30.0 41.0 54-55 36.347780364302096 39.0 35.0 40.0 29.0 41.0 56-57 36.16678032316645 39.0 35.0 40.0 28.5 41.0 58-59 35.930809210404135 38.5 35.0 40.0 28.0 41.0 60-61 35.64070095773798 38.0 34.0 40.0 27.5 41.0 62-63 35.34972869805809 38.0 34.0 40.0 27.0 41.0 64-65 35.06468396446971 37.5 34.0 40.0 26.0 41.0 66-67 34.74212735684589 37.0 34.0 40.0 26.0 41.0 68-69 34.356055823035845 36.5 33.0 39.0 26.0 40.5 70-71 33.97495634646 36.0 33.0 39.0 25.5 40.0 72-73 33.56559458976891 35.5 32.0 38.5 25.0 40.0 74-75 33.13476332203867 35.0 32.0 38.0 24.0 40.0 76-77 31.588932159841328 34.0 30.0 36.0 22.5 38.5 78-79 32.33853479521009 35.0 31.0 36.0 23.5 39.0 80-81 32.254422953411314 35.0 31.0 36.0 23.5 38.5 82-83 31.932322704258603 34.5 31.0 36.0 22.5 37.5 84-85 31.58722709050368 34.0 31.0 35.5 21.5 37.0 86-87 31.269970959755458 34.0 30.5 35.0 20.5 36.5 88-89 30.96040931805403 34.0 30.0 35.0 20.0 36.0 90-91 30.665434287517627 34.0 30.0 35.0 20.0 36.0 92-93 30.42860747093044 34.0 29.0 35.0 19.0 35.5 94-95 30.191288896399087 34.0 29.0 35.0 18.5 35.0 96-97 29.976211268872405 34.0 29.0 35.0 18.0 35.0 98-99 29.804059669884225 34.0 29.0 35.0 18.0 35.0 100 27.797020182009703 31.0 25.0 34.0 10.0 35.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.2728621424695348 1101 2 0.19500126304970422 1101 3 0.24584769548520313 1101 4 0.10962339459130988 1101 5 0.11593576875779377 1101 6 0.16368665687799933 1101 7 0.17564597060849252 1101 8 0.1459868762213432 1101 9 0.20233539764814168 1101 10-11 0.1799723250738552 1101 12-13 0.18494360062458526 1101 14-15 0.2568308708255742 1101 16-17 0.28655681007344214 1101 18-19 0.29478472674831124 1101 20-21 0.2792946728805461 1101 22-23 0.2719837476610891 1101 24-25 0.2545303329010835 1101 26-27 0.23263694677694247 1101 28-29 0.31875784110171423 1101 30-31 0.36269506993472334 1101 32-33 0.40744469464808475 1101 34-35 0.37260485927371434 1101 36-37 0.3976943378431912 1101 38-39 0.4258152274789708 1101 40-41 0.4180898440065519 1101 42-43 0.40799823560989523 1101 44-45 0.4293113430666793 1101 46-47 0.4804781037319259 1101 48-49 0.48057367043460175 1101 50-51 0.4500929806118279 1101 52-53 0.4129065394477891 1101 54-55 0.4096858645890151 1101 56-57 0.4150871920200956 1101 58-59 0.46634509669993207 1101 60-61 0.4076257450633136 1101 62-63 0.46375010480344514 1101 64-65 0.46117029931645703 1101 66-67 0.42492354808183563 1101 68-69 0.4357055576068163 1101 70-71 0.4361799656238716 1101 72-73 0.2935089803536215 1101 74-75 0.38737652865751926 1101 76-77 0.4160422602059519 1101 78-79 0.5507067810672197 1101 80-81 0.509856512758752 1101 82-83 0.5366817841253209 1101 84-85 0.46930303973464405 1101 86-87 0.4683338449618546 1101 88-89 0.4372781893587714 1101 90-91 0.392531850497182 1101 92-93 0.40315828691075595 1101 94-95 0.42551100898575456 1101 96-97 0.36698882823807466 1101 98-99 0.3447033590942574 1101 100 0.5461196813247575 1102 1 0.27304420081012637 1102 2 0.20244175708383239 1102 3 0.24962248925793773 1102 4 0.1621331704794855 1102 5 0.1384049431858756 1102 6 0.12028718443429653 1102 7 0.14520820945196533 1102 8 0.13349187204386226 1102 9 0.20132940630635687 1102 10-11 0.20457685290143246 1102 12-13 0.2099606905024558 1102 14-15 0.32903773919061763 1102 16-17 0.3057484787154223 1102 18-19 0.2641049226072596 1102 20-21 0.313175240834461 1102 22-23 0.29165765366988694 1102 24-25 0.2811110824520924 1102 26-27 0.287517811818212 1102 28-29 0.3334787102403851 1102 30-31 0.3509108722965095 1102 32-33 0.3924579005827127 1102 34-35 0.3749113212932045 1102 36-37 0.37823719593299643 1102 38-39 0.4149237208520944 1102 40-41 0.36190179136641376 1102 42-43 0.28475459557694904 1102 44-45 0.33998105020236125 1102 46-47 0.39883312978279406 1102 48-49 0.3794840051610251 1102 50-51 0.40330249021175035 1102 52-53 0.41030242249353677 1102 54-55 0.41862857875243975 1102 56-57 0.4189628575208957 1102 58-59 0.48657166682391306 1102 60-61 0.43700934530043156 1102 62-63 0.49764697842818606 1102 64-65 0.4494558809162186 1102 66-67 0.494589359810945 1102 68-69 0.5026722202381393 1102 70-71 0.5110950142579682 1102 72-73 0.5296216309673341 1102 74-75 0.5163841483840272 1102 76-77 0.5040883021283626 1102 78-79 0.5318338214404363 1102 80-81 0.5268782141736992 1102 82-83 0.5132739294596576 1102 84-85 0.5006077673535607 1102 86-87 0.5015764453009481 1102 88-89 0.5067948498652974 1102 90-91 0.49407830238160955 1102 92-93 0.4011670625312611 1102 94-95 0.4817727079398466 1102 96-97 0.5849757662106398 1102 98-99 0.5376201592999514 1102 100 0.6954618155554861 1103 1 0.2618415414326378 1103 2 0.19872872316254586 1103 3 0.2426979759169967 1103 4 0.1142356799817037 1103 5 0.1284243445907478 1103 6 0.14953886835329655 1103 7 0.14721274752157143 1103 8 0.11679958322811501 1103 9 0.16524986763943872 1103 10-11 0.18818348397335427 1103 12-13 0.22534885050611564 1103 14-15 0.30129057878177434 1103 16-17 0.2889653488659931 1103 18-19 0.2551196399746729 1103 20-21 0.24537102665668442 1103 22-23 0.30093018996149823 1103 24-25 0.2688167751734625 1103 26-27 0.26820194373134854 1103 28-29 0.33814031743479234 1103 30-31 0.37293190548012234 1103 32-33 0.4198826997617999 1103 34-35 0.4042950513654162 1103 36-37 0.4304512688019386 1103 38-39 0.43963621520252616 1103 40-41 0.45586036797950413 1103 42-43 0.49146957411854686 1103 44-45 0.43669536112459895 1103 46-47 0.5246824467826556 1103 48-49 0.48525138728171413 1103 50-51 0.4789863707565374 1103 52-53 0.5116261536807301 1103 54-55 0.5509099542499527 1103 56-57 0.6068044291404533 1103 58-59 0.6396345931545753 1103 60-61 0.6297621191873901 1103 62-63 0.5973350265853838 1103 64-65 0.6188782507820818 1103 66-67 0.6061666637884358 1103 68-69 0.7007051758718745 1103 70-71 0.6737922544078216 1103 72-73 0.6431887113464683 1103 74-75 0.6239554405516898 1103 76-77 0.6343420248685909 1103 78-79 0.7116069671136174 1103 80-81 0.7012497093275201 1103 82-83 0.6800373480491615 1103 84-85 0.5929085262121809 1103 86-87 0.614899328182215 1103 88-89 0.6738922336933051 1103 90-91 0.692653131003155 1103 92-93 0.6839776461587697 1103 94-95 0.7790787984204997 1103 96-97 0.7660592160196025 1103 98-99 0.7774024407190012 1103 100 0.8987787950139641 1104 1 0.17830002070237327 1104 2 0.15094284370335487 1104 3 0.21538676621037212 1104 4 0.01024554660909871 1104 5 0.08015668844211632 1104 6 0.08764035849662122 1104 7 0.10009440271896608 1104 8 0.11098629790785708 1104 9 0.1484217629751754 1104 10-11 0.18808581645162548 1104 12-13 0.2235961211637445 1104 14-15 0.2895216268671561 1104 16-17 0.22905638332954226 1104 18-19 0.25716686441358405 1104 20-21 0.12716297723599013 1104 22-23 0.2043606116314649 1104 24-25 0.21100711720194454 1104 26-27 0.23744475893406758 1104 28-29 0.3117404846978076 1104 30-31 0.2662031325100642 1104 32-33 0.28771043353201264 1104 34-35 0.36397154892164707 1104 36-37 0.3091078151937907 1104 38-39 0.38571615617788524 1104 40-41 0.44642753860767925 1104 42-43 0.4883293192041407 1104 44-45 0.43391496174309907 1104 46-47 0.4223421513708985 1104 48-49 0.505358552278075 1104 50-51 0.4802102775768091 1104 52-53 0.5352726702617545 1104 54-55 0.5469285646236415 1104 56-57 0.6148472142935475 1104 58-59 0.632557848932656 1104 60-61 0.606673436207025 1104 62-63 0.617851673782809 1104 64-65 0.7441219376231274 1104 66-67 0.645754068415684 1104 68-69 0.6842156892628637 1104 70-71 0.666913021837594 1104 72-73 0.6638119190650471 1104 74-75 0.6608782724508657 1104 76-77 0.625791561387846 1104 78-79 0.7064705758654171 1104 80-81 0.7811338130226275 1104 82-83 0.653061709146229 1104 84-85 0.6594954813387268 1104 86-87 0.7365148889163038 1104 88-89 0.7787216050191894 1104 90-91 0.6625117086804586 1104 92-93 0.6527464582729507 1104 94-95 0.6476418837415565 1104 96-97 0.664765838082797 1104 98-99 0.8122014937575983 1104 100 1.0896933336625665 1105 1 0.18121461204445666 1105 2 0.11082958584136549 1105 3 0.19559745942696338 1105 4 0.10869415549194628 1105 5 0.06405986680145048 1105 6 0.10896204318255087 1105 7 0.12908157630985073 1105 8 0.10787395447088954 1105 9 0.12854267806658015 1105 10-11 0.15456154062128036 1105 12-13 0.19640091263422477 1105 14-15 0.26860139726226606 1105 16-17 0.2953228198004467 1105 18-19 0.3103945061741058 1105 20-21 0.24173203583961111 1105 22-23 0.31573525441420003 1105 24-25 0.3111423475016011 1105 26-27 0.3114590862156845 1105 28-29 0.3554988974486264 1105 30-31 0.3851838917732806 1105 32-33 0.40308413959922973 1105 34-35 0.4316837419500317 1105 36-37 0.39286363646014877 1105 38-39 0.4221893111691699 1105 40-41 0.45205303975384226 1105 42-43 0.41622950595760955 1105 44-45 0.40374452985471265 1105 46-47 0.4920820014121787 1105 48-49 0.5194660506288287 1105 50-51 0.5866589745609261 1105 52-53 0.5383828864305755 1105 54-55 0.4900291605488576 1105 56-57 0.5803351295074322 1105 58-59 0.6017256553158958 1105 60-61 0.6684909847973373 1105 62-63 0.6394838031299415 1105 64-65 0.6075004650509825 1105 66-67 0.6357904240717644 1105 68-69 0.6738927920301876 1105 70-71 0.7388778169263404 1105 72-73 0.6821852978410377 1105 74-75 0.7569074891588343 1105 76-77 0.7170793567228522 1105 78-79 0.78911746063077 1105 80-81 0.7547291683106536 1105 82-83 0.7129571413013593 1105 84-85 0.7134749905248121 1105 86-87 0.7515086976495695 1105 88-89 0.772678747901292 1105 90-91 0.8440243418688951 1105 92-93 0.822282911061933 1105 94-95 0.886242152063577 1105 96-97 0.9446211145126959 1105 98-99 0.960544898213989 1105 100 1.197273351234152 1106 1 0.22470491697214356 1106 2 0.18336136954773252 1106 3 0.2036166448583856 1106 4 0.15536513156683895 1106 5 0.1610724552333238 1106 6 0.1253341265286565 1106 7 0.18006359543564088 1106 8 0.15269987553634934 1106 9 0.15519709167902818 1106 10-11 0.18147499589844784 1106 12-13 0.22575350844426367 1106 14-15 0.2856859447550235 1106 16-17 0.27959857389132026 1106 18-19 0.28689481895630564 1106 20-21 0.339031812578952 1106 22-23 0.3405657310595913 1106 24-25 0.37738841748796403 1106 26-27 0.3559674438891065 1106 28-29 0.36482349272584713 1106 30-31 0.440921686457024 1106 32-33 0.44285699579400273 1106 34-35 0.44039611882519836 1106 36-37 0.39626918488810503 1106 38-39 0.41152985647612894 1106 40-41 0.4881929402472167 1106 42-43 0.4654403291707325 1106 44-45 0.5176202117645445 1106 46-47 0.547088506831301 1106 48-49 0.5584823195286788 1106 50-51 0.5573687940876297 1106 52-53 0.5735460580242417 1106 54-55 0.5660572101794727 1106 56-57 0.6181612587453955 1106 58-59 0.6801336220299632 1106 60-61 0.7173275511616666 1106 62-63 0.690596598601708 1106 64-65 0.7272493372185522 1106 66-67 0.7271953330298473 1106 68-69 0.6745710690343216 1106 70-71 0.7325604119186693 1106 72-73 0.7336312387287407 1106 74-75 0.750539332573787 1106 76-77 0.6825266397432337 1106 78-79 0.7853400962090333 1106 80-81 0.7786638132361858 1106 82-83 0.7619091049182032 1106 84-85 0.7028616332846802 1106 86-87 0.691061928479435 1106 88-89 0.78644641239935 1106 90-91 0.8032629962911813 1106 92-93 0.9057020574229817 1106 94-95 0.9278495450223403 1106 96-97 0.9246252585420898 1106 98-99 0.9713256047977765 1106 100 1.0629272993810481 1107 1 0.23865317198151104 1107 2 0.2146535230828448 1107 3 0.252666348768976 1107 4 0.1554155166827229 1107 5 0.13561318421314184 1107 6 0.15384332619105834 1107 7 0.19037313836700065 1107 8 0.19052053210844377 1107 9 0.20513035222533915 1107 10-11 0.17715615847229316 1107 12-13 0.20506441049109725 1107 14-15 0.2895968838914982 1107 16-17 0.2496818308170603 1107 18-19 0.2504665792038594 1107 20-21 0.25440876279714786 1107 22-23 0.35845849378208783 1107 24-25 0.34063939925503206 1107 26-27 0.3823133068151492 1107 28-29 0.41374386685306774 1107 30-31 0.3367507837118069 1107 32-33 0.3571889063700979 1107 34-35 0.3509412119890527 1107 36-37 0.43516033166742574 1107 38-39 0.4473724457370807 1107 40-41 0.4091607622481561 1107 42-43 0.4323341824600817 1107 44-45 0.48766382220168936 1107 46-47 0.45355797119166397 1107 48-49 0.5042121426247306 1107 50-51 0.43215067574148947 1107 52-53 0.4528277304379742 1107 54-55 0.49492324003380617 1107 56-57 0.5580061408497912 1107 58-59 0.6732583426035816 1107 60-61 0.5972819791328163 1107 62-63 0.6396483286058015 1107 64-65 0.7387057718110341 1107 66-67 0.7665996874140291 1107 68-69 0.6551878006921328 1107 70-71 0.7561493870685325 1107 72-73 0.7082713078434111 1107 74-75 0.7152878543419376 1107 76-77 0.6630714205709616 1107 78-79 0.7565764885695714 1107 80-81 0.7586668269074508 1107 82-83 0.739073272990538 1107 84-85 0.7016622611208199 1107 86-87 0.6804929574885037 1107 88-89 0.6567607799912096 1107 90-91 0.5600581921326757 1107 92-93 0.684680728787896 1107 94-95 0.8408564118243831 1107 96-97 0.8828375131001387 1107 98-99 0.9560237833209229 1107 100 0.971798718415581 1108 1 0.22079498577991785 1108 2 0.1605469035871181 1108 3 0.20886880287967102 1108 4 0.12374091659754782 1108 5 0.10531370377627525 1108 6 0.07918040697592943 1108 7 0.12922292825133042 1108 8 0.1345159005197729 1108 9 0.13564834167085849 1108 10-11 0.14043350058540938 1108 12-13 0.19615893727677758 1108 14-15 0.24212897039085846 1108 16-17 0.3046568228917508 1108 18-19 0.2699679507362447 1108 20-21 0.2924115650005561 1108 22-23 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64-65 -0.6991384647338421 2316 66-67 -0.3919066085377736 2316 68-69 -0.44335597609060784 2316 70-71 -0.6688245632179459 2316 72-73 -0.7077988608793149 2316 74-75 -0.7995036453674942 2316 76-77 -0.766330826357521 2316 78-79 -0.6899994810892593 2316 80-81 -0.6235996632684362 2316 82-83 -0.43950425708563756 2316 84-85 -0.47895685209352123 2316 86-87 -0.5716888167796093 2316 88-89 -0.5326892754422374 2316 90-91 -0.4962853380490415 2316 92-93 -0.4037551892880238 2316 94-95 -0.36881127875729547 2316 96-97 -0.36989052441188264 2316 98-99 -0.5320793153721155 2316 100 -0.7755176485438575 >>END_MODULE >>Per sequence quality scores pass #Quality Count 9 1.0 10 14.0 11 36.0 12 122.0 13 333.0 14 1060.0 15 2995.0 16 7187.0 17 15728.0 18 29842.0 19 46368.0 20 67092.0 21 95245.0 22 126550.0 23 139580.0 24 140743.0 25 151528.0 26 174495.0 27 212067.0 28 260235.0 29 319008.0 30 391143.0 31 482639.0 32 599306.0 33 762463.0 34 1008795.0 35 1377900.0 36 2018265.0 37 3203876.0 38 3526263.0 39 616118.0 40 74.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.71080056243071 22.490947148614072 13.92123142992045 22.877020859034765 2 14.125862195264652 22.842051820983674 41.62727414852321 21.40481183522846 3 16.08959702735991 29.922389318291643 31.5683092653131 22.419704389035346 4 10.425797343997335 18.774677627641655 41.70951506318525 29.09000996517576 5 10.773580589548729 40.71973119481486 36.91819477220287 11.588493443433542 6 27.26703485725463 41.558634754674756 19.269075299230384 11.90525508884023 7 25.082272875618038 34.09431319666369 24.928397672799978 15.895016254918293 8 24.73722414176028 37.43115903815087 21.556614512924682 16.275002307164176 9 26.609863952498635 14.914620040498031 21.791907657337745 36.68360834966558 10-11 22.903814022260534 27.99030314308657 30.660760796474833 18.445122038178063 12-13 24.045743344883217 26.03946258465846 30.31657460373982 19.598219466718504 14-15 21.53012114859596 27.18514418804352 27.90141148505955 23.383323178300966 16-17 20.113060473329615 30.12682738842825 28.86164028483316 20.898471853408974 18-19 20.03809305165472 29.097880679041175 31.056254232059505 19.807772037244593 20-21 20.918804947225976 28.37165976343954 31.046881992672866 19.662653296661624 22-23 20.73493552764008 28.440215550782526 30.52690515242024 20.297943769157154 24-25 20.4510687445439 28.793274134735974 30.772749021791952 19.982908098928174 26-27 20.30075245157857 28.846762874263682 30.84718076030604 20.005303913851712 28-29 20.226448381727636 28.34930415883966 31.32472246265024 20.099524996782467 30-31 20.642136807614428 28.56049746425614 30.861706051091765 19.935659677037666 32-33 20.46398139269846 28.687855956312518 30.829052072523215 20.019110578465803 34-35 20.6549092442908 28.815337396500375 30.937789503979207 19.591963855229622 36-37 20.745242928795285 28.429514994290212 30.721952165943335 20.10328991097117 38-39 20.4345001087987 28.986714896002958 30.785988045897394 19.792796949300946 40-41 20.44147861904178 28.626137898309572 31.16756508555844 19.764818397090206 42-43 20.3765626087489 28.485099095323267 30.79280695729253 20.345531338635304 44-45 20.27798092228752 28.8674297857303 30.59001458678822 20.264574705193958 46-47 20.52505074779146 28.860521286182045 30.260430743907097 20.353997222119393 48-49 20.371412346712166 29.084117656623015 30.228253108274473 20.316216888390347 50-51 20.542027167916128 28.925281178693098 29.975448119751942 20.557243533638832 52-53 20.52573275740625 29.055669384024007 29.934737722633308 20.48386013593643 54-55 20.518731946459727 29.177205425423995 29.908411045058646 20.39565158305763 56-57 20.52332479361358 29.238393192293792 29.711708712605873 20.52657330148676 58-59 20.359055715433858 29.02488997892708 30.10442902106465 20.511625284574414 60-61 20.69435897669944 29.496472854979345 29.663134957731817 20.146033210589394 62-63 20.40810142640349 29.114595035969675 30.138919911027713 20.33838362659912 64-65 20.23094267066532 29.018472732444987 29.98165717621423 20.768927420675457 66-67 20.323629215291874 29.151798065278662 29.618767417574286 20.905805301855178 68-69 20.267620070351565 29.298932162474394 29.7951984695978 20.638249297576237 70-71 20.404332788083767 28.87625091418994 29.929396456524366 20.790019841201925 72-73 20.396016897373425 29.225872583337807 29.490804448266577 20.887306071022188 74-75 20.37217115198976 29.368560136158738 29.34202714522734 20.91724156662416 76-77 20.15798228500615 29.208619942257442 29.767140899177193 20.866256873559216 78-79 20.23882043603881 29.100091533412865 29.524834460174727 21.1362535703736 80-81 20.110196137829604 29.57209556201669 29.220301979532604 21.097406320621104 82-83 20.55994217725165 29.304691467312466 29.3166221795427 20.818744175893183 84-85 20.191618281093398 29.38110525137993 29.189502818820195 21.237773648706472 86-87 19.926838542816398 29.436541016683186 29.59545838789177 21.04116205260864 88-89 20.283578857793714 29.198141150971367 29.3997678261339 21.118512165101023 90-91 20.299145231413 29.343017460477565 29.383227283161915 20.974610024947523 92-93 20.35977833604303 29.246007460338518 29.205752763309956 21.188461440308494 94-95 19.964647636206955 28.94949375631895 29.341729614842414 21.74412899263168 96-97 20.260327287391476 28.954154191011742 29.253275114983968 21.532243406612807 98-99 19.899491584482085 28.950098610720577 29.382168913487394 21.768240891309944 100 18.866244225112037 29.536566112593547 29.492982650549003 22.104207011745412 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 7716.0 1 5445.0 2 4446.0 3 5824.5 4 4885.5 5 4767.0 6 7425.0 7 14662.5 8 23296.0 9 26751.5 10 23038.5 11 18378.5 12 18360.5 13 19427.5 14 20451.5 15 21582.0 16 22725.0 17 24239.0 18 26238.0 19 28752.5 20 32580.5 21 37654.0 22 44553.5 23 54721.0 24 65693.0 25 79290.5 26 101384.5 27 129225.0 28 157017.5 29 194992.5 30 247123.0 31 292684.0 32 348061.5 33 424301.5 34 505452.5 35 574268.5 36 654409.5 37 725060.0 38 746822.5 39 751912.5 40 713319.0 41 662373.5 42 633618.0 43 615529.5 44 609689.0 45 616626.5 46 598028.0 47 552416.5 48 521135.5 49 496154.0 50 473197.5 51 428104.5 52 374813.0 53 337416.5 54 305415.5 55 267584.5 56 228698.5 57 191399.0 58 157582.5 59 130259.5 60 102194.0 61 77139.0 62 58096.5 63 42688.5 64 30156.0 65 20164.5 66 13357.0 67 8872.0 68 6018.5 69 4090.5 70 2839.0 71 2017.5 72 1263.0 73 915.0 74 717.0 75 447.5 76 285.0 77 188.0 78 133.5 79 90.5 80 63.5 81 43.5 82 34.5 83 34.0 84 29.0 85 17.5 86 11.0 87 8.0 88 7.0 89 4.0 90 2.5 91 3.0 92 1.5 93 2.0 94 3.0 95 2.0 96 1.0 97 1.0 98 2.5 99 4.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.003486071654237976 2 0.06278731964887527 3 7.542591397351258E-4 4 8.176422607212708E-4 5 0.0018507871327954346 6 6.148162735656067E-4 7 0.0 8 8.049656365240417E-4 9 5.070649678891602E-5 10-11 0.0 12-13 0.0 14-15 0.0 16-17 7.352442034392823E-4 18-19 0.0011282195535533815 20-21 0.003248384950539932 22-23 0.0 24-25 5.070649678891602E-5 26-27 2.693782641911163E-4 28-29 0.0014609809387306427 30-31 0.0021518569574796235 32-33 0.0029694992182008944 34-35 0.0039646142176833704 36-37 0.001365906257251425 38-39 0.0014451351584841064 40-41 0.005305167226540338 42-43 0.006648889391446612 44-45 0.0051245253317298255 46-47 0.00643338678009372 48-49 0.008344387877825992 50-51 0.008198606699557858 52-53 0.004135748644345963 54-55 0.0024529267821638123 56-57 0.0037839723228728574 58-59 0.0041927934532334935 60-61 0.007219337480321917 62-63 0.012971355709814579 64-65 0.0038124947273166228 66-67 0.009618388609647506 68-69 0.011862151092557041 70-71 0.005127694487779132 72-73 0.007149616047237158 74-75 0.003077250523877341 76-77 0.003118449552518335 78-79 0.008943358371145063 80-81 0.007609143674386709 82-83 0.016837726089969425 84-85 0.01737331346230235 86-87 0.02196225142169925 88-89 0.019753349655332094 90-91 0.022577067695264855 92-93 0.031682053024924585 94-95 0.03527587598483901 96-97 0.02123334553035858 98-99 0.016926462459350027 100 0.01736063683810512 >>END_MODULE >>Sequence Length Distribution pass #Length Count 100 1.5777071E7 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.60152319997688 #Duplication Level Percentage of deduplicated Percentage of total 1 64.16564396353996 21.560633742823445 2 16.474651442986794 11.071467653461067 3 7.243089082037605 7.301364774887574 4 3.8739169797474737 5.206780450790763 5 2.226838234814931 3.7412578304864734 6 1.4787280669386125 2.9812509268616854 7 0.9958001691569336 2.342228174052732 8 0.7094140208676154 1.906991334445765 9 0.49958656828036124 1.5108182698022454 >10 2.1347999964246025 11.530748776449043 >50 0.07758087431878584 1.7886019371187354 >100 0.08086587283997196 6.120040672596249 >500 0.018967609937483178 4.512487947816158 >1k 0.018481220592649936 12.016785355350471 >5k 0.0013264033917944908 3.209084547357695 >10k+ 3.0949412475204783E-4 3.1994576056998425 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 67707 0.4291480972608921 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 58511 0.3708609792020331 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 44769 0.28375989434287263 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 6.338312098614502E-6 0.0 6 0.0 0.0 0.0 6.338312098614502E-6 0.0 7 0.0 0.0 0.0 6.338312098614502E-6 0.0 8 0.0 0.0 0.0 1.2676624197229003E-5 0.0 9 0.0 0.0 0.0 4.436818469030152E-5 0.0 10-11 0.0 0.0 0.0 6.021396493683777E-5 0.0 12-13 0.0 0.0 0.0 6.655227703545227E-5 0.0 14-15 0.0 0.0 0.0 7.605974518337403E-5 0.0 16-17 0.0 0.0 0.0 9.190552542991028E-5 0.0 18-19 0.0 0.0 0.0 1.0775130567644654E-4 0.0 20-21 0.0 1.2676624197229003E-5 0.0 1.8064189481051333E-4 0.0 22-23 0.0 1.2676624197229003E-5 0.0 3.486071654237976E-4 0.0 24-25 0.0 1.2676624197229003E-5 0.0 5.989704933190705E-4 0.0 26-27 0.0 1.2676624197229003E-5 0.0 9.444085026935609E-4 0.0 28-29 0.0 1.2676624197229003E-5 0.0 0.002078966368345557 0.0 30-31 0.0 2.5353248394458007E-5 0.0 0.006449232560340256 0.0 32-33 0.0 2.5353248394458007E-5 0.0 0.016276785469242043 0.0 34-35 0.0 2.5353248394458007E-5 0.0 0.03167254555677666 0.0 36-37 0.0 2.8522404443765258E-5 0.0 0.05693388842580477 0.0 38-39 0.0 3.169156049307251E-5 0.0 0.09811073297445388 0.0 40-41 0.0 3.169156049307251E-5 0.0 0.14607590978071913 0.0 42-43 0.0 3.169156049307251E-5 0.0 0.19135998056927042 0.0 44-45 0.0 3.169156049307251E-5 0.0 0.24765369947311514 0.0 46-47 0.0 3.169156049307251E-5 0.0 0.3160060571445739 0.0 48-49 0.0 3.169156049307251E-5 0.0 0.39127668247167047 0.0 50-51 0.0 3.169156049307251E-5 0.0 0.47602942269829424 0.0 52-53 0.0 3.169156049307251E-5 0.0 0.5605951827180089 0.0 54-55 0.0 3.169156049307251E-5 0.0 0.6502949755376014 0.0 56-57 0.0 3.169156049307251E-5 0.0 0.7577737337938075 0.0 58-59 0.0 5.070649678891601E-5 0.0 0.8829110295567536 0.0 60-61 0.0 5.387565283822327E-5 0.0 1.0219102138793696 0.0 62-63 0.0 5.7044808887530516E-5 0.0 1.1643732857638784 0.0 64-65 0.0 5.7044808887530516E-5 0.0 1.308503333730323 0.0 66-67 0.0 6.338312098614502E-5 0.0 1.4742597025772401 0.0 68-69 0.0 6.338312098614502E-5 0.0 1.6664214796269854 0.0 70-71 0.0 6.338312098614502E-5 0.0 1.8868964968212414 0.0 72-73 0.0 6.338312098614502E-5 0.0 2.1140774482158315 0.0 74-75 0.0 6.338312098614502E-5 0.0 2.3362194414920237 0.0 76-77 0.0 6.338312098614502E-5 0.0 2.5753956485332417 0.0 78-79 0.0 6.655227703545227E-5 0.0 2.8577928057749125 0.0 80-81 0.0 6.972143308475952E-5 0.0 3.1704807565358615 0.0 82-83 0.0 6.972143308475952E-5 0.0 3.5003075032114643 3.169156049307251E-6 84-85 0.0 6.972143308475952E-5 0.0 3.8214982996527045 6.338312098614502E-6 86-87 0.0 6.972143308475952E-5 0.0 4.152500803222601 6.338312098614502E-6 88 0.0 6.972143308475952E-5 0.0 4.422246689515437 6.338312098614502E-6 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 29200 0.0 54.77164 1 GTATCAA 54445 0.0 38.418354 1 GTACATG 74905 0.0 31.48416 1 ATCAACG 67955 0.0 29.638838 3 TACATGG 78765 0.0 29.593824 2 ACATGGG 75705 0.0 29.429022 3 CAACGCA 68625 0.0 29.369823 5 TCAACGC 68975 0.0 29.241327 4 AACGCAG 71570 0.0 28.325182 6 TATCAAC 74150 0.0 27.358006 2 CATGGGG 27290 0.0 26.432236 4 TACCTGG 9235 0.0 25.102991 2 CATGGGA 42625 0.0 24.959799 4 ACGCAGA 80485 0.0 24.901386 7 CGCAGAG 80630 0.0 24.728388 8 GCAGAGT 88645 0.0 22.105335 9 ATGGGGG 15995 0.0 22.034576 5 GAGTACT 51045 0.0 21.03628 12-13 AGAGTAC 84485 0.0 20.874678 10-11 ATGGGCG 2290 0.0 20.5207 5 >>END_MODULE